Fetal-derived trophoblast use the apoptotic cytokine tumor necrosis factor-alpha-related apoptosis-inducing ligand to induce smooth muscle cell death.

Remodeling of the uterine spiral arteries during pregnancy transforms them from high to low resistance vessels that lack vasoconstrictive properties. This process is essential to meet the demand for increased blood flow imposed by the growing fetus. Loss of endothelial and smooth muscle cells (SMC) is evident in remodeled arteries but the mechanisms underlying this transformation remain unknown. This study investigated the hypothesis that fetal trophoblast invading from the placenta instigate remodeling by triggering cell death in vascular SMC. Specifically, a role for trophoblast-derived death inducing cytokine tumor necrosis factor-α–related apoptosis-inducing ligand (TRAIL) was investigated. Expression of the activating TRAIL receptors R1 and R2 was detected by flow cytometry on human aortic SMC and by immunohistochemistry on spiral artery SMC. Recombinant human TRAIL induced human aortic SMC apoptosis, which was inhibited by antibodies against TRAIL-R1 or -R2. Perfusion of denuded spiral artery segments with recombinant human TRAIL also induced SMC apoptosis. Trophoblasts isolated from first trimester placenta expressed membrane-associated TRAIL and induced apoptosis of human aortic SMC; apoptosis was significantly inhibited by a recombinant human TRAIL-R1:Fc construct. Trophoblast within the first trimester placental bed also expressed TRAIL. These data show that: 1) TRAIL causes SMC death; 2) trophoblast produce the apoptotic cytokine TRAIL; and 3) trophoblast induce SMC apoptosis via a TRAIL-dependent mechanism. We conclude that TRAIL produced by trophoblast causes apoptosis of SMC and thus may contribute to SMC loss during spiral artery remodeling in pregnancy

Adaptive mutation using statistics mechanism for genetic algorithms

Copyright @ 2004 Springer-Verla

The PIKfyve Inhibitor YM201636 Blocks the Continuous Recycling of the Tight Junction Proteins Claudin-1 and Claudin-2 in MDCK cells

Tight junctions mediate the intercellular diffusion barrier found in epithelial tissues but they are not static complexes; instead there is rapid movement of individual proteins within the junctions. In addition some tight junction proteins are continuously being endocytosed and recycled back to the plasma membrane. Understanding the dynamic behaviour of tight junctions is important as they are altered in a range of pathological conditions including cancer and inflammatory bowel disease. In this study we investigate the effect of treating epithelial cells with a small molecule inhibitor (YM201636) of the lipid kinase PIKfyve, a protein which is involved in endocytic trafficking. We show that MDCK cells treated with YM201636 accumulate the tight junction protein claudin-1 intracellularly. In contrast YM201636 did not alter the localization of other junction proteins including ZO-1, occludin and E-cadherin. A biochemical trafficking assay was used to show that YM201636 inhibited the endocytic recycling of claudin-1, providing an explanation for the intracellular accumulation. Claudin-2 was also found to constantly recycle in confluent MDCK cells and treatment with YM201636 blocked this recycling and caused accumulation of intracellular claudin-2. However, claudin-4 showed negligible endocytosis and no detectable intracellular accumulation occurred following treatment with YM201636, suggesting that not all claudins show the same rate of endocytic trafficking. Finally, we show that, consistent with the defects in claudin trafficking, incubation with YM201636 delayed formation of the epithelial permeability barrier. Therefore, YM201636 treatment blocks the continuous recycling of claudin-1/claudin-2 and delays epithelial barrier formation

Photovoltaic restoration of sight with high visual acuity

Patients with retinal degeneration lose sight due to the gradual demise of photoreceptors. Electrical stimulation of surviving retinal neurons provides an alternative route for the delivery of visual information. We demonstrate that subretinal implants with 70-μm-wide photovoltaic pixels provide highly localized stimulation of retinal neurons in rats. The electrical receptive fields recorded in retinal ganglion cells were similar in size to the natural visual receptive fields. Similarly to normal vision, the retinal response to prosthetic stimulation exhibited flicker fusion at high frequencies, adaptation to static images and nonlinear spatial summation. In rats with retinal degeneration, these photovoltaic arrays elicited retinal responses with a spatial resolution of 64 ± 11 μm, corresponding to half of the normal visual acuity in healthy rats. The ease of implantation of these wireless and modular arrays, combined with their high resolution, opens the door to the functional restoration of sight in patients blinded by retinal degeneration

Management of venous thrombosis in fibular free osseomusculocutaneous flaps used for mandibular reconstruction: clinical techniques and treatment considerations

<p>Abstract</p> <p>Background</p> <p>Mandibular reconstruction by means of fibula transplants is the standard therapy for severe bone loss after subtotal mandibulectomy. Venous failure still represents the most common complication in free flap surgery. We present the injection of heparine into the arterial pedicle as modification of the revising both anastomoses in these cases and illustrate the application with a clinical case example.</p> <p>Methods</p> <p>Methods consist of immediate revision surgery with clot removal, heparin perfusion by direct injection in the arterial vessel of the pedicle, subsequent high dose low-molecular weight heparin therapy, and leeches. After 6 hours postoperatively, images of early flap recovery show first sings of recovery by fading livid skin color.</p> <p>Results</p> <p>The application of this technique in a patient with venous thrombosis resulted in the complete recovery of the flap 60 hours postoperatively. Other cases achieved similar success without additional lysis Therapy or revision of the arterial anastomosis.</p> <p>Conclusion</p> <p>Rescue of fibular flaps is possible even in patients with massive thrombosis if surgical revision is done quickly.</p

PARP16 is a tail-anchored endoplasmic reticulum protein required for the PERK- and IRE1α-mediated unfolded protein response

Poly(ADP-ribose) polymerases (PARPs; also known as ADP-ribosyl transferase D proteins) modify acceptor proteins with ADP-ribose modifications of varying length (reviewed in refs 1, 2, 3). PARPs regulate key stress response pathways, including DNA damage repair and the cytoplasmic stress response. Here, we show that PARPs also regulate the unfolded protein response (UPR) of the endoplasmic reticulum (ER). Human PARP16 (also known as ARTD15) is a tail-anchored ER transmembrane protein required for activation of the functionally related ER stress sensors PERK and IRE1α during the UPR. The third identified ER stress sensor, ATF6, is not regulated by PARP16. As is the case for other PARPs that function during stress, the enzymatic activity of PARP16 is upregulated during ER stress when it ADP-ribosylates itself, PERK and IRE1α. ADP-ribosylation by PARP16 is sufficient for activating PERK and IRE1α in the absence of ER stress, and is required for PERK and IRE1α activation during the UPR. Modification of PERK and IRE1α by PARP16 increases their kinase activities and the endonuclease activity of IRE1α. Interestingly, the carboxy-terminal luminal tail of PARP16 is required for PARP16 function during ER stress, suggesting that it transduces stress signals to the cytoplasmic PARP catalytic domain.National Cancer Institute (U.S.) (Cancer Center Support Core Grant P30-CA14051)National Institutes of Health (U.S.) (Grant 5R01 GM087465-02)Kathy and Curt Marble Cancer Research FundJeptha H. and Emily V. Wade FundVirginia and D.K. Ludwig Fund for Cancer Researc

Data envelopment analysis in financial services: a citations network analysis of banks, insurance companies and money market funds

Development and application of the data envelopment analysis (DEA) method, have been the subject of numerous reviews. In this paper, we consider the papers that apply DEA methods specifically to financial services, or which use financial services data to experiment with a newly introduced DEA model. We examine 620 papers published in journals indexed in the Web of Science database, from 1985 to April 2016. We analyse the sample applying citations network analysis. This paper investigates the DEA method and its applications in financial services. We analyse the diffusion of DEA in three sub-samples: (1) banking groups, (2) money market funds, and (3) insurance groups by identifying the main paths, that is, the main flows of the ideas underlying each area of research. This allows us to highlight the main approaches, models and efficiency types used in each research areas. No unique methodological preference emerges within these areas. Innovations in the DEA methodologies (network models, slacks based models, directional distance models and Nash bargaining game) clearly dominate recent research. For each subsample, we describe the geographical distribution of these studies, and provide some basic statistics related to the most active journals and scholars

Nanotechnology researchers' collaboration relationships: A gender analysis of access to scientific information

Women are underrepresented in science, technology, engineering, and mathematics fields, particularly at higher levels of organizations. This article investigates the impact of this underrepresentation on the processes of interpersonal collaboration in nanotechnology. Analyses are conducted to assess: (1) the comparative tie strength of women's and men's collaborations, (2) whether women and men gain equal access to scientific information through collaborators, (3) which tie characteristics are associated with access to information for women and men, and (4) whether women and men acquire equivalent amounts of information by strengthening ties. Our results show that the overall tie strength is less for women's collaborations and that women acquire less strategic information through collaborators. Women and men rely on different tie characteristics in accessing information, but are equally effective in acquiring additional information resources by strengthening ties. This article demonstrates that the underrepresentation of women in science, technology, engineering, and mathematics has an impact on the interpersonal processes of scientific collaboration, to the disadvantage of women scientists.Villanueva-Felez, Á.; Woolley, RD.; Cañibano Sánchez, C. (2015). Nanotechnology researchers' collaboration relationships: A gender analysis of access to scientific information. Social Studies of Science. 45(1):100-129. doi:10.1177/0306312714552347S100129451ACKER, J. (1990). HIERARCHIES, JOBS, BODIES: Gender & Society, 4(2), 139-158. doi:10.1177/089124390004002002Aitken, C., Power, R., & Dwyer, R. (2008). A very low response rate in an on-line survey of medical practitioners. Australian and New Zealand Journal of Public Health, 32(3), 288-289. doi:10.1111/j.1753-6405.2008.00232.xAngrist, J. D., & Pischke, J.-S. (2009). Mostly Harmless Econometrics. doi:10.1515/9781400829828Baruch, Y., & Holtom, B. C. (2008). Survey response rate levels and trends in organizational research. Human Relations, 61(8), 1139-1160. doi:10.1177/0018726708094863Beaver, D. D. (2001). Scientometrics, 52(3), 365-377. doi:10.1023/a:1014254214337Boardman, P. C., & Corley, E. A. (2008). University research centers and the composition of research collaborations. Research Policy, 37(5), 900-913. doi:10.1016/j.respol.2008.01.012Bourdieu, P. (1975). The specificity of the scientific field and the social conditions of the progress of reason. Social Science Information, 14(6), 19-47. doi:10.1177/053901847501400602Bourdieu, P. (1977). Outline of a Theory of Practice. doi:10.1017/cbo9780511812507Bourdieu, P. (1989). Social Space and Symbolic Power. Sociological Theory, 7(1), 14. doi:10.2307/202060Bouty, I. (2000). INTERPERSONAL AND INTERACTION INFLUENCES ON INFORMAL RESOURCE EXCHANGES BETWEEN R&D RESEARCHERS ACROSS ORGANIZATIONAL BOUNDARIES. Academy of Management Journal, 43(1), 50-65. doi:10.2307/1556385Bozeman, B., & Corley, E. (2004). Scientists’ collaboration strategies: implications for scientific and technical human capital. Research Policy, 33(4), 599-616. doi:10.1016/j.respol.2004.01.008Bozeman, B., & Gaughan, M. (2011). How do men and women differ in research collaborations? An analysis of the collaborative motives and strategies of academic researchers. Research Policy, 40(10), 1393-1402. doi:10.1016/j.respol.2011.07.002Bozeman, B., & Rogers, J. D. (2002). A churn model of scientific knowledge value: Internet researchers as a knowledge value collective. Research Policy, 31(5), 769-794. doi:10.1016/s0048-7333(01)00146-9Bozeman, B., Dietz, J. S., & Gaughan, M. (2001). Scientific and technical human capital: an alternative model for research evaluation. International Journal of Technology Management, 22(7/8), 716. doi:10.1504/ijtm.2001.002988Brass, D. J. (1985). MEN’S AND WOMEN’S NETWORKS: A STUDY OF INTERACTION PATTERNS AND INFLUENCE IN AN ORGANIZATION. Academy of Management Journal, 28(2), 327-343. doi:10.2307/256204Chompalov, I., Genuth, J., & Shrum, W. (2002). The organization of scientific collaborations. Research Policy, 31(5), 749-767. doi:10.1016/s0048-7333(01)00145-7Cook, C., Heath, F., & Thompson, R. L. (2000). A Meta-Analysis of Response Rates in Web- or Internet-Based Surveys. Educational and Psychological Measurement, 60(6), 821-836. doi:10.1177/00131640021970934Durbin, S. (2010). Creating Knowledge through Networks: a Gender Perspective. Gender, Work & Organization, 18(1), 90-112. doi:10.1111/j.1468-0432.2010.00536.xEcklund, E. H., Lincoln, A. E., & Tansey, C. (2012). Gender Segregation in Elite Academic Science. Gender & Society, 26(5), 693-717. doi:10.1177/0891243212451904Ensign, P. C. (2009). Knowledge Sharing among Scientists. doi:10.1057/9780230617131Etzkowitz, H., Kemelgor, C., & Uzzi, B. (2000). Athena Unbound. doi:10.1017/cbo9780511541414Flyvbjerg, B. (2001). Making Social Science Matter. doi:10.1017/cbo9780511810503FOX, M. F. (2001). WOMEN, SCIENCE, AND ACADEMIA. Gender & Society, 15(5), 654-666. doi:10.1177/089124301015005002Fox, M. F. (2010). Women and Men Faculty in Academic Science and Engineering: Social-Organizational Indicators and Implications. American Behavioral Scientist, 53(7), 997-1012. doi:10.1177/0002764209356234Fox, M. F., & Stephan, P. E. (2001). Careers of Young Scientists: Social Studies of Science, 31(1), 109-122. doi:10.1177/030631201031001006Fox, M. F., Sonnert, G., & Nikiforova, I. (2009). Successful Programs for Undergraduate Women in Science and Engineering: Adapting versus Adopting the Institutional Environment. Research in Higher Education, 50(4), 333-353. doi:10.1007/s11162-009-9120-4Friedkin, N. (1980). A test of structural features of granovetter’s strength of weak ties theory. Social Networks, 2(4), 411-422. doi:10.1016/0378-8733(80)90006-4Gaughan, M. (2005). Introduction to the Symposium: Women in Science. The Journal of Technology Transfer, 30(4), 339-342. doi:10.1007/s10961-005-2579-zGaughan, M., & Corley, E. A. (2010). Science faculty at US research universities: The impacts of university research center-affiliation and gender on industrial activities. Technovation, 30(3), 215-222. doi:10.1016/j.technovation.2009.12.001Granovetter, M. S. (1973). The Strength of Weak Ties. American Journal of Sociology, 78(6), 1360-1380. doi:10.1086/225469Hansen, M. T. (1999). The Search-Transfer Problem: The Role of Weak Ties in Sharing Knowledge across Organization Subunits. Administrative Science Quarterly, 44(1), 82. doi:10.2307/2667032Ibarra, H. (1992). Homophily and Differential Returns: Sex Differences in Network Structure and Access in an Advertising Firm. Administrative Science Quarterly, 37(3), 422. doi:10.2307/2393451Islam, N., & Miyazaki, K. (2009). Nanotechnology innovation system: Understanding hidden dynamics of nanoscience fusion trajectories. Technological Forecasting and Social Change, 76(1), 128-140. doi:10.1016/j.techfore.2008.03.021Katz, J. S., & Martin, B. R. (1997). What is research collaboration? Research Policy, 26(1), 1-18. doi:10.1016/s0048-7333(96)00917-1Koch, N. S., & Emrey, J. A. . (2001). The Internet and Opinion Measurement: Surveying Marginalized Populations. Social Science Quarterly, 82(1), 131-138. doi:10.1111/0038-4941.00012Kyvik, S., & Teigen, M. (1996). Child Care, Research Collaboration, and Gender Differences in Scientific Productivity. Science, Technology, & Human Values, 21(1), 54-71. doi:10.1177/016224399602100103Lee, S., & Bozeman, B. (2005). The Impact of Research Collaboration on Scientific Productivity. Social Studies of Science, 35(5), 673-702. doi:10.1177/0306312705052359Levin, D. Z., & Cross, R. (2004). The Strength of Weak Ties You Can Trust: The Mediating Role of Trust in Effective Knowledge Transfer. Management Science, 50(11), 1477-1490. doi:10.1287/mnsc.1030.0136Lin, N. (2001). Social Capital. doi:10.1017/cbo9780511815447McFadyen, M. A., & Cannella, A. A. (2004). SOCIAL CAPITAL AND KNOWLEDGE CREATION: DIMINISHING RETURNS OF THE NUMBER AND STRENGTH OF EXCHANGE RELATIONSHIPS. Academy of Management Journal, 47(5), 735-746. doi:10.2307/20159615McFadyen, M. A., Semadeni, M., & Cannella, A. A. (2009). Value of Strong Ties to Disconnected Others: Examining Knowledge Creation in Biomedicine. Organization Science, 20(3), 552-564. doi:10.1287/orsc.1080.0388Manfreda, K. L., Bosnjak, M., Berzelak, J., Haas, I., & Vehovar, V. (2008). Web Surveys versus other Survey Modes: A Meta-Analysis Comparing Response Rates. International Journal of Market Research, 50(1), 79-104. doi:10.1177/147078530805000107Marsden, P. V., & Campbell, K. E. (1984). Measuring Tie Strength. Social Forces, 63(2), 482. doi:10.2307/2579058Mason, M. A., & Ekman, E. M. (2007). Mothers on the Fast TrackHow a New Generation Can Balance Family and Careers. doi:10.1093/acprof:oso/9780195182675.001.0001Mayer, R. C., Davis, J. H., & Schoorman, F. D. (1995). An Integrative Model Of Organizational Trust. Academy of Management Review, 20(3), 709-734. doi:10.5465/amr.1995.9508080335Nahapiet, J., & Ghoshal, S. (1998). Social Capital, Intellectual Capital, and the Organizational Advantage. Academy of Management Review, 23(2), 242-266. doi:10.5465/amr.1998.533225Oliver, A. L., & Liebeskind, J. P. (1997). Three Levels of Networking for Sourcing Intellectual Capital in Biotechnology. International Studies of Management & Organization, 27(4), 76-103. doi:10.1080/00208825.1997.11656719Podolny, J. M., & Baron, J. N. (1997). Resources and Relationships: Social Networks and Mobility in the Workplace. American Sociological Review, 62(5), 673. doi:10.2307/2657354Rhoton, L. A. (2011). Distancing as a Gendered Barrier. Gender & Society, 25(6), 696-716. doi:10.1177/0891243211422717Rothstein, M. G., & Davey, L. M. (1995). Gender differences in network relationships in academia. Women in Management Review, 10(6), 20-25. doi:10.1108/09649429510095999Rowley, T., Behrens, D., & Krackhardt, D. (2000). Redundant governance structures: an analysis of structural and relational embeddedness in the steel and semiconductor industries. Strategic Management Journal, 21(3), 369-386. doi:10.1002/(sici)1097-0266(200003)21:33.0.co;2-mSCOTT, D. B. (1996). SHATTERING THE INSTRUMENTAL-EXPRESSIVE MYTH. Gender & Society, 10(3), 232-247. doi:10.1177/089124396010003003Shapin, S. (1994). A Social History of Truth. doi:10.7208/chicago/9780226148847.001.0001Shrum, W., Chompalov, I., & Genuth, J. (2001). Trust, Conflict and Performance in Scientific Collaborations. Social Studies of Science, 31(5), 681-730. doi:10.1177/030631201031005002Smith-Doerr, L. (2004). Flexibility and Fairness: Effects of the Network Form of Organization on Gender Equity in Life Science Careers. Sociological Perspectives, 47(1), 25-54. doi:10.1525/sop.2004.47.1.25Stix, G. (2001). Little Big Science. Scientific American, 285(3), 32-37. doi:10.1038/scientificamerican0901-32Uzzi, B. (1996). The Sources and Consequences of Embeddedness for the Economic Performance of Organizations: The Network Effect. American Sociological Review, 61(4), 674. doi:10.2307/2096399Uzzi, B. (1997). Social Structure and Competition in Interfirm Networks: The Paradox of Embeddedness. Administrative Science Quarterly, 42(1), 35. doi:10.2307/2393808Vinck, D. (2010). The Sociology of Scientific Work. doi:10.4337/9781849807197Fan, W., & Yan, Z. (2010). Factors affecting response rates of the web survey: A systematic review. Computers in Human Behavior, 26(2), 132-139. doi:10.1016/j.chb.2009.10.015Ziman, J. M. (Ed.). (1994). Prometheus Bound. doi:10.1017/cbo978051158506

A Comparative Analysis of Detecting Symmetries in Toroidal Topology

In late 1940s and with the introduction of cellular automata, various types of problems in computer science and other multidisciplinary fields have started utilising this new technique. The generative capabilities of cellular automata have been used for simulating various natural, physical and chemical phenomena. Aside from these applications, the lattice grid of cellular automata has been providing a by-product interface to generate graphical patterns for digital art creation. One notable aspect of cellular automata is symmetry, detecting of which is often a difficult task and computationally expensive. This paper uses a swarm intelligence algorithm—Stochastic Diffusion Search—to extend and generalise previous works and detect partial symmetries in cellular automata generated patterns. The newly proposed technique tailored to address the spatially-independent symmetry problem is also capable of identifying the absolute point of symmetry (where symmetry holds from all perspectives) in a given pattern. Therefore, along with partially symmetric areas, the centre of symmetry is highlighted through the convergence of the agents of the swarm intelligence algorithm. Additionally this paper proposes the use of entropy and information gain measure as a complementary tool in order to offer insight into the structure of the input cellular automata generated images. It is shown that using these technique provides a comprehensive picture about both the structure of the images as well as the presence of any complete or spatially-independent symmetries. These technique are potentially applicable in the domain of aesthetic evaluation where symmetry is one of the measures

Epstein-Barr Virus Stimulates Torque Teno Virus Replication: A Possible Relationship to Multiple Sclerosis

Viral infections have been implicated in the pathogenesis of multiple sclerosis. Epstein-Barr virus (EBV) has frequently been investigated as a possible candidate and torque teno virus (TTV) has also been discussed in this context. Nevertheless, mechanistic aspects remain unresolved. We report viral replication, as measured by genome amplification, as well as quantitative PCR of two TTV-HD14 isolates isolated from multiple sclerosis brain in a series of EBV-positive and -negative lymphoblastoid and Burkitt's lymphoma cell lines. Our results demonstrate the replication of both transfected TTV genomes up to day 21 post transfection in all the evaluated cell lines. Quantitative amplification indicates statistically significant enhanced TTV replication in the EBV-positive cell lines, including the EBV-converted BJAB line, in comparison to the EBV-negative Burkitt's lymphoma cell line BJAB. This suggests a helper effect of EBV infections in the replication of TTV. The present study provides information on a possible interaction of EBV and TTV in the etiology and progression of multiple sclerosis