Intergenomic and epistatic interactions control free radical mediated pancreatic β-cell damage.

Alloxan (AL)-generated Reactive Oxygen Species (ROS) selectively destroy insulin-producing pancreatic β-cells. A previous genome-wide scan (GWS) using a cohort of 296 F2 hybrids between NOD (AL-sensitive) and ALR (AL-resistant) mice identified linkages contributing to β-cell susceptibility or resistance to AL-induced diabetes on Chromosomes (Chr) 2, 3, 8, and a single nucleotide polymorphism i

Proceedings of the Tenth Annual Beef Cattle Short Course.

91 p

Journal ofOrgonomy major articles WilhelmReich and Orgonomy '. Functional Cosmology, Part III • A Childhood Experience of the Emotional Plague • Orgonomic FirstAid asTreatmentfor a Ticof Recent Onset • Reliefof severeSymptomsin an Adolescent • Notesfrom t

Introduction Reich left an astounding legacy to medical science. The following partial list of his accomplishments is to orient us functionally and historically with regard to the biopathies. To those acquainted with his work, the overwhelming logic of his discoveries speaks for itself

Proceedings of the Tenth Annual Beef Cattle Short Course.

91 p

SARS-CoV-2 infects human pancreatic β cells and elicits β cell impairment.

Emerging evidence points toward an intricate relationship between the pandemic of coronavirus disease 2019 (COVID-19) and diabetes. While preexisting diabetes is associated with severe COVID-19, it is unclear whether COVID-19 severity is a cause or consequence of diabetes. To mechanistically link COVID-19 to diabetes, we tested whether insulin-producing pancreatic β cells can be infected by SARS-CoV-2 and cause β cell depletion. We found that the SARS-CoV-2 receptor, ACE2, and related entry factors (TMPRSS2, NRP1, and TRFC) are expressed in β cells, with selectively high expression of NRP1. We discovered that SARS-CoV-2 infects human pancreatic β cells in patients who succumbed to COVID-19 and selectively infects human islet β cells in vitro. We demonstrated that SARS-CoV-2 infection attenuates pancreatic insulin levels and secretion and induces β cell apoptosis, each rescued by NRP1 inhibition. Phosphoproteomic pathway analysis of infected islets indicates apoptotic β cell signaling, similar to that observed in type 1 diabetes (T1D). In summary, our study shows SARS-CoV-2 can directly induce β cell killing