Dietary factors associated with faecal consistency and other indicators of gastrointestinal health in the captive cheetah (Acinonyx jubatus)

Gastrointestinal diseases pose significant risks to captive cheetah survival and welfare. Multiple factors are thought to be associated with these diseases, but to date a comprehensive epidemiological survey of disease risk factors has not been conducted. A survey of diet and health parameters was completed for 184 captive cheetahs in 86 international facilities. Comparisons were made among dietary factors with respect to disease status and observed faecal consistency, incidence of vomiting and diarrhoea in the past 4 weeks. Extremely dry faeces were most common in cheetahs fed carcasses, but was still of low incidence (15%). Contrastingly, cheetahs fed commercially prepared diets had the highest prevalence of liquid faeces “ always ” or “ often ” (9%). Cheetahs fed raw meat diets had the highest prevalence of soft faeces with no shape (22%), as well as of firm and dry faeces (40%)

Preliminary investigation of the influence of long-term dietary isoflavone intake on puberty onset and oestrous cycles in domestic cats (Felis catus)

Genistein and daidzein are isoflavones which are reported to influence the reproductive system in a variety of mammalian species. This pilot study aimed to determine if dietary isoflavones could potentially influence reproductive parameters in domestic cats, when consumed during the postnatal development period. Cats (n=12) were maintained on either a treatment (150µg/g DM genistein and 150µg/g DM daidzein, n=4) or control (isoflavone free, n= 8) diet from weaning, up to 414 (±17.2) days post-weaning. Vaginal smears were taken thrice weekly and examined for oestrogen-induced cellular degradation in all cats. Behavioural indicators of oestrous were routinely scored for the presence or absence of six key behaviours. Genistein and daidzein did not alter puberty onset or oestrous cycle parameters in these cats (P>0.05). Behavioural scores were higher in cats in the treatment group than control. Incidence of apparent spontaneous ovulation (inferred from extended inter-oestrous periods) was greater in treated cats than control cats, although serum hormone profiles were not available to confirm this observation. Further testing is warranted

Preliminary investigation of the effects of long-term dietary intake of genistein and daidzein on hepatic histopathology and biochemistry in domestic cats (Felis catus)

Dietary isoflavones have been hypothesised to play a role in hepatic veno-occlusive disease in captive exotic felids, although empirical evidence is lacking. This study aimed to investigate the effect of long-term (>1 year) dietary genistein and daidzein exposure on the hepatic biochemistry and histology of domestic cats. Individual cats were assessed for hepatic enzyme and bile acid production before and after the removal of isoflavones from their diet in the treatment group (n=4), and at the same times in unexposed control animals (n=7). No significant differences were detectable in hepatic biochemistry between treatment and control groups, and all serum values were within the normal reference ranges for domestic cats. Additionally, treatment animals demonstrated slightly greater areas of fibrosis surrounding hepatic venules than control animals, but this difference was not statistically significant. On the basis of the results presented, dietary isoflavones, at the current dose and duration of exposure do not appear to modulate hepatic enzyme production or histological parameters

Preliminary investigation of the influence of long-term dietary isoflavone intake on reproductive tract histology and sex steroid receptor expression in female domestic cats (Felis catus)

Genistein and daidzein are isoflavones which are reported to influence the reproductive system in a variety of mammalian species. This pilot study aimed to determine if dietary isoflavones could potentially influence reproductive tract histology or morphology in domestic cats, when consumed during the postnatal development period. Cats were maintained on either treatment (150 µg/g DM genistein and 150 µg/g DM daidzein, n=4) or control (isoflavone free, n=8) diets from weaning, up to 414 (±17.2) days post-weaning. Reproductive tissues were collected during routine ovario-hysterectomy and examined for histology and sex steroid receptor expression. Findings indicate that these dietary isoflavones influenced the expression of oestrogen receptor α (ERα) and oestrogen receptor β (ERβ), and progesterone receptor in feline reproductive tissues. One cat in the treatment group developed suppurative endometritis, but no evidence of uterotrophic or histological changes were found in any other cats. The potential to alter expression of hormone receptors in the reproductive tract of domestic cats exposed to genistein and daidzein warrants further investigation

Feeding practices and other factors associated with faecal consistency and frequencies of vomiting and diarrhoea in captive tigers (Panthera tigris)

Gastrointestinal (GI) health is important to the welfare of captive tigers, and diet is considered a likely influencing factor. A survey was performed to collect information on GI health indicators and diet of tigers housed in zoological facilities across the globe. Completed surveys were received for one tiger from each of 32 facilities. Three (9%) tigers were reported as being diagnosed as having current GI disease; 24 (75%) had ideal (soft with shape) faeces ‘often’ to ‘always’ during the four weeks before survey completion. Potential associations between current GI disease and other variables could not be explored because of the low disease prevalence. Commercial raw meat diets were the most commonly fed diet type, and the most common food source was horse. Upon multivariate analysis, including country as a covariate, the odds and frequency of vomiting during the previous six months increased with the frequency of feeding muscle meat and chicken, and decreased as the frequency of feeding long bones increased. The odds and frequency of diarrhoea over the previous six months increased with the frequency of feeding beef and muscle meat; and the frequency of liquid faeces in the previous four weeks increased with oral antimicrobial treatment and increasing frequency of feeding beef. Although limited by the small sample size, these findings characterised the nutritional care that captive tigers currently receive and provided preliminary insight into dietary associations with indicators of GI health. The findings support the need to consider species-specific dietary adaptations and for further investigations into the health impact of diet in captive tigers

Nutritional composition of browse and diets fed to ungulates at the Breeding Centre for Endangered Arabian Wildlife

Captive browsing ruminants are particularly susceptible to gastrointestinal disorders, and inappropriate diets are an underlying factor. This study investigated the nutritional composition of browse and pelleted feedstuffs used in an Arabian facility and compared nutrient intake against current recommendations for exotic ungulates. Additionally, retrospective evaluation of post-mortem findings with regard to gastrointestinal pathologies was conducted (n = 497). Samples of browse from seven species of locally cultivated plants, as well as three brands of pelleted feeds, were submitted for laboratory analysis. Following a five-day feed intake study, nutrient intake was calculated. Only moderate variation was seen among browse species’ composition compared to previous reports. However, significant variation occurred between plant fractions (stem, leaves and seed pods) for neutral detergent fibre and several minerals. Browse comprised over half of the metabolisable energy (ME) intake of Arabian tahr (rabitragus jayakari), but only 11% of ME for Arabian gazelle (Gazella gazella cora) and Dorcas gazelle (G. gazella dorcas). However, no relationship could be detected between gastrointestinal disease and browse provision in these species. No nutrient deficiencies were identified, but the Arabian tahr diet exceeded the recommended amount of crude protein, and both gazelle diets provided excess iron, manganese, copper and zinc. These mineral excesses are the subject of on-going investigations in order to better balance the diets offered. Moreover, the high starch content of pelleted diets evaluated in the current study (22-29%, on a dry matter basis) indicates that a reduction in the proportional provision of pellets would improve the diet suitability, particularly for the gazelles. This study highlights a number of areas for potential improvement, although further research is required to fully understand the implications of these findings

Conservation education: are zoo animals effective ambassadors and is there any cost to their welfare?

Animal ambassador encounters (AAE), where visitors come into close-contact with animals, are popular in zoos and are advocated as promoting connection to wild species. However, educational and animal-welfare implications are relatively unknown. We conducted a systematic literature review (PRISMA) to investigate visitor and animal outcomes of AAE. We identified 19 peer reviewed articles and 13 other records focused on AAEs. Although we found net positive or neutral impacts overall, several studies indicated that high-intensity visitor contact and long-term exposure may be detrimental to animal welfare. Most studies lacked rigour and claims were based on an absence of negative impacts rather than evidence of benefits. Multiple publications were derived from the same datasets and there were no standardised measures for either welfare or education impacts. Of the peer-reviewed articles, just two considered both education and welfare. Education studies often used perceived learning or only post-experience testing. Welfare studies used small samples (median n = 4; range 1–59), and limited measures of welfare. In order to justify the continued use of AAEs in modern zoos, animal welfare costs must be proven to be minimal whilst having demonstrable and substantial visitor educational value. Large-scale, standardised impact assessments of both education and welfare impacts are needed

Perceived Efficacy of Livestock-Guarding Dogs in South Africa: Implications for Cheetah Conservation

Large wild carnivore predation on domestic livestock and the associated financial losses may increase efforts toward lethal control of carnivore populations. Livestock-guarding dogs could provide an effective alternative to such lethal control by mitigating depredation losses. Although this information is available in North America, the cost-effectiveness of guarding dogs has not been studied in other areas experiencing large carnivore depredation such as South Africa, where the socio-economic context is very different from that of North America. We assessed the costs and benefits of 97 livestock-guarding dogs working on 94 farms in South Africa between 2005 and 2011 by reviewing data collected from questionnaires on perceived depredation losses prior to and during guarding dog placement, rates of guarding dog behavioral problems, removals, and pre-senile mortality. Perceived livestock depredation ceased in 91% of guarding dog placements, with gross mean annual financial savings US$3,189/farm. Estimated annual program costs per year of the livestock-guarding dog program were US$ 2,780. However, 16% of guarding dogs had reported behavioral problems, with inattentiveness cited as the most common problem. Twelve percent of guarding dogs were removed from the program because of behavioral problems. Premature death was observed in 22% of guarding dogs, most often due to snake bites. Participating farmer tolerance toward cheetahs (Acinonyx jubatus), as well as cheetah-sighting frequency, appeared to increase during participation in the livestockguarding dog program. If further corrective behavioral and snake-aversion training were implemented, guarding dogs may offer a cost-effective method of non-lethal predator control and could potentially contribute to the long-term mitigation of human–carnivore conflict in South Africa

Suspected adverse reactions to oral administration of a praziquantel-pyrantel combination in captive cheetahs (Acinonyx jubatus)

Objective: To characterize adverse reactions to oral administration of a combination of praziquantel and pyrantel embonate or pyrantel pamoate, with or without oxantel embonate, in captive cheetahs (Acinonyx jubatus). Design: Retrospective case series and case-control study. Animals: 16 captive cheetahs with signs of adverse reaction to oral administration of praziquantel and pyrantel, with or without oxantel embonate (affected group), and 27 cheetahs without such reactions (unaffected group), all from 3 independent facilities. Procedures: Medical records and postmortem findings for affected cheetahs were reviewed and compared with those of unaffected animals. Anthelmintic doses administered, age, and sex of cheetahs were compared between groups. Results: 3 reactions in affected cheetahs were fatal, whereas the remainder ranged from mild to severe. Postmortem examination failed to reveal any disease processes or conditions to explain the deaths. No differences in anthelmintic dose were identified between affected and unaffected cheetahs for all facilities combined, and no correlation existed between dose and reaction severity. No association with sex was detected, but affected cheetahs were significantly younger than unaffected cheetahs. This difference was not significant after controlling for facility. Conclusions and clinical relevance: Cheetahs were concluded to have had an adverse reaction to the praziquantel-pyrantel combination because of temporal proximity of onset of clinical signs to dose administration, similarity of signs to those reported for toxicosis in other species for these drugs, and a lack of other disease process or environmental explanatory factors. A highly cautious approach to the use of this drug combination is recommended for cheetahs