Dimer-dimer stacking interactions are important for nucleic acid binding by the archaeal chromatin protein Alba

Archaea use a variety of small basic proteins to package their DNA. One of the most widespread and highly conserved is the Alba (Sso10b) protein. Alba interacts with both DNA and RNA in vitro, and we show in the present study that it binds more tightly to dsDNA (double-stranded DNA) than to either ssDNA (single-stranded DNA) or RNA. The Alba protein is dimeric in solution, and forms distinct ordered complexes with DNA that have been visualized by electron microscopy studies; these studies suggest that, on binding dsDNA, the protein forms extended helical protein fibres. An end-to-end association of consecutive Alba dimers is suggested by the presence of a dimer-dimer interface in crystal structures of Alba from several species, and by the strong conservation of the interface residues, centred on Are and Phe(60). In the present study we map perturbation of the polypeptide backbone of Alba upon binding to DNA and RNA by NMR, and demonstrate the central role of Phe(60) in forming the dimer dimer interface. Site-directed spin labelling and pulsed ESR are used to confirm that an end-to-end, dimer dimer interaction forms in the presence of dsDNA.Publisher PDFPeer reviewe

White\u27s Mount Vernon Directory, and City Guide (1876-1877)

To which is added biographical sketches of a few of our aged and venerable citizens, under the head of \u27three score and ten\u27 .https://digital.kenyon.edu/mtvernondirectories/1032/thumbnail.jp

Choosing terrace systems (1993)

Terraces are one way to control soil erosion. Crop rotation and tillage practices also control erosion. but they do not provide control of runoff water after heavy rains, Terraces provide this control and should often be a part of your water management plan for continuous row crops on slopes of 5 percent or more. Terraces do require high capital investments, however. Costs may range from $100 to$250 per acre, depending on the type of terrace system

Laying the Foundation: Digital Humanities in Academic Libraries

Laying the Foundation: Digital Humanities in Academic Libraries examines the library’s role in the development, implementation, and instruction of successful digital humanities projects. It pays special attention to the critical role of librarians in building sustainable programs. It also examines how libraries can support the use of digital scholarship tools and techniques in undergraduate education. Academic libraries are nexuses of research and technology; as such, they provide fertile ground for cultivating and curating digital scholarship. However, adding digital humanities to library service models requires a clear understanding of the resources and skills required. Integrating digital scholarship into existing models calls for a reimagining of the roles of libraries and librarians. In many cases, these reimagined roles call for expanded responsibilities, often in the areas of collaborative instruction and digital asset management, and in turn these expanded responsibilities can strain already stretched resources. Laying the Foundation provides practical solutions to the challenges of successfully incorporating digital humanities programs into existing library services. Collectively, its authors argue that librarians are critical resources for teaching digital humanities to undergraduate students and that libraries are essential for publishing, preserving, and making accessible digital scholarship.https://docs.lib.purdue.edu/purduepress_ebooks/1032/thumbnail.jp

Coastal wetland area change for two freshwater diversions in the Mississippi River Delta

Coastal systems around the globe are being re-integrated with adjacent river systems to restore the natural hydrologic connection to riparian wetlands. The Mississippi River sediment diversions or river reconnections are one such tool to combat high rates of wetland loss in coastal Louisiana, USA by providing freshwater, sediment, and nutrients. There has been some disagreement in the published literature whether re-establishing river reconnection is slowing or contributing to coastal wetland loss. This issue is due to the difficulties in the application of remote sensing in low-relief environments where water level changes could indicate either land loss or simply temporary submergence. We analyzed land change at the receiving areas of two existing freshwater river diversions, Davis Pond and Caernarvon, which have been intermittently receiving river water for up to 2+ decades. This study provides a robust analysis of wetland land change rates in proximity these river diversions including years before river reconnection. Our analyses indicate a net land gain since river reconnection operations began at Davis Pond Diversion (+3.42 km2; range: +2.02–4.81 km2) and no statistically significant change at the Caernarvon Diversion. The Davis Pond wetland results are corroborated with data from a decadal field study documenting increased inorganic sedimentation in the soil. It is clear from this study and others, that river reconnection can increase or, in the case of Caernarvon, have no statistical effect on the land change in these systems due to differences in vegetation, hydroperiod, sediment delivery and external factors including hurricane impacts. Our remote sensing analysis was compared with a global water area change analysis mapping tool which also supported our findings

The Value of a Chest CT in the Evaluation of a Newly Detected Brain Tumor

OBJECTIVE: To create a care pathway for patients with a newly detected brain tumor, by examining common diagnostic pathways for patients diagnosed with a primary brain tumor or a central nervous system metastasis. MATERIALS AND METHODS: All patients diagnosed atMGH between 1/1/95 and 12/31/97 with a primary brain tumor or a central nervous system metastasis were studied. Only patients who displayed one or two brain lesions and presented to the MGH emergency ward or transferred in from another emergency ward were included in the study. Clinical characteristics, use and results of radiological testing, and final diagnostic procedures were evaluated. RESULTS: Forty-eight patients were selected for the study. The most common ancillary test performed was a chest x-ray; 97.9% of the patients received a chest x-ray, while only 18.8% received a chest CT. None of the chest CT examinations showed a diagnostically significant different from the chest x-ray. Other ancillary scanning was done infrequently. DISCUSSION: A relatively small proportion of patients with a newly detected brain lesion received any type of ancillary testing beyond a chest x-ray. A chest x-ray appeared to change the biopsy site from the brain to another site in about 50% of the cases. Not enough chest CT examinations were done to determine their effectiveness. However, the resuhs seem to indicate that the preliminary diagnosis of the admitting physician is not necessarily based upon the results of a chest x-ray or other ancillary testing

Sustainability Reporting In The Accounting Curriculum

Seven class sections of undergraduate students in principles of managerial accounting courses participated in a study to examine the impact of sustainability accounting lectures with Internet assignments or lectures alone influence students' knowledge, skills, and attitudes toward sustainability reporting. The results of this study show that lectures with Internet assignments and lectures alone were equally effective in causing changes in the dependent measures compared to control groups who received no instruction on sustainability