Exobiology site selection for future Mars missions: Martian paleolake sediments and terrestrial analogs

This research was conducted to establish the scientific framework for the exobiological study of sediments on Mars and to encourage the selection of these sedimentary deposits as sampling sites for future Mars missions. A study was completed on the Antarctic Dry Valley Lakes (terrestrial analogs of the purported Martian paleolakes) and their sediments that allowed the development of quantitative models relating environmental factors to the nature of the biological community and sediment forming processes. The publications presented include: (1) Diversity of micro-fungi isolated in an Antarctic dry valley; (2) Lake Hoare, Antarctica--sedimentation through a thick perennial ice cover; (3) The possibility of life on Mars during a water-rich past; (4) An Antarctic research outpost as a model for planetary exploration; (5) Early Martian environments--the Antarctic and other terrestrial analogs; (6) Lipophilic pigments from the benthos of a perennially ice-covered Antarctic lake; and (7) Perennially ice-covered Lake Hoare, Antarctica--physical environment, biology, and sedimentation

Paleolakes and life on early Mars

Two distinct directions have begun to elucidate key parameters in the search for extinct life on Mars. Carbonate sediments, deposited about 10,000 years ago in association with biological activity, have been sampled from the paleolake beds of Lake Vanda and Meirs in the McMurdo Dry Valleys in Antarctica. These samples are being analyzed for simple biological signatures that remain in cold and dry paleolake sediments, namely microfossils, percent carbonate, and total organic carbon. Our second initiative is the study of Colour Lake, in the Canadian Arctic, that periodically maintains a perennial ice cover. Physical measurements started this year will be used to determine one end point for ice covered lake environments and will be compared to continuous measurements from Antarctic lakes started in November 1985. Interestingly, Colour Lake also supports benthic mat communities, but the low pH precludes carbonate deposition. This research will broaden our knowledge base for what conditions are necessary for ice covered lake formation and what biological signatures will remain in paleolake deposits

Operation of an experimental algal gas exchanger for use in a CELSS

Concepts of a Closed Ecological Life Support System (CELSS) anticipate the use of photosynthetic organisms (higher plants and algae) for air revitalization. The rates of production and uptake of carbon dioxide and oxygen between the crew and the photosynthetic organisms are mismatched. An algal system used for gas exchange only will have the difficulty of an accumulation or depletion of these gases beyond physiologically tolerable limits (in a closed system the mismatch between assimilatory quotient (AQ) and respiratory quotient (RQ) is balanced by the operation of the waste processor). The results are given of a study designed to test the feasibility of using environmental manipulations to maintain physiologically appropriate atmospheres for algae and mice in a gas closed system. Specifically, the atmosphere behavior of this system is considered with algae grown on nitrate or urea and at different light intensities and optical densities. Manipulation of both allow operation of the system in a gas stable manner. Operation of such a system in a CELSS may be useful for reduction of buffer sizes, as a backup system for higher plant air revitalization and to supply extra oxygen to the waste processor or during crew changes

Fourth Symposium on Chemical Evolution and the Origin and Evolution of Life

This symposium was held at the NASA Ames Research Center, Moffett Field, California, July 24-27, 1990. The NASA exobiology investigators reported their recent research findings. Scientific papers were presented in the following areas: cosmic evolution of biogenic compounds, prebiotic evolution (planetary and molecular), early evolution of life (biological and geochemical), evolution of advanced life, solar system exploration, and the Search for Extraterrestrial Intelligence (SETI)

Life on ice, Antarctica and Mars

The study of the origin of life and the prospects for human exploration of Mars are two themes developed in a new 57-minute film, Life on Ice, Antarctica, and Mars, produced by the InnerSpace Foundation and WHRO Television for broadcast by the Public Broadcasting System (PBS). A brief explanation of the film and how it relates to the future human exploration of space is presented

Systematic Review of Potential Health Risks Posed by Pharmaceutical, Occupational and Consumer Exposures to Metallic and Nanoscale Aluminum, Aluminum Oxides, Aluminum Hydroxide and Its Soluble Salts

Aluminum (Al) is a ubiquitous substance encountered both naturally (as the third most abundant element) and intentionally (used in water, foods, pharmaceuticals, and vaccines); it is also present in ambient and occupational airborne particulates. Existing data underscore the importance of Al physical and chemical forms in relation to its uptake, accumulation, and systemic bioavailability. The present review represents a systematic examination of the peer-reviewed literature on the adverse health effects of Al materials published since a previous critical evaluation compiled by Krewski et al. (2007). Challenges encountered in carrying out the present review reflected the experimental use of different physical and chemical Al forms, different routes of administration, and different target organs in relation to the magnitude, frequency, and duration of exposure. Wide variations in diet can result in Al intakes that are often higher than the World Health Organization provisional tolerable weekly intake (PTWI), which is based on studies with Al citrate. Comparing daily dietary Al exposures on the basis of “total Al”assumes that gastrointestinal bioavailability for all dietary Al forms is equivalent to that for Al citrate, an approach that requires validation. Current occupational exposure limits (OELs) for identical Al substances vary as much as 15-fold. The toxicity of different Al forms depends in large measure on their physical behavior and relative solubility in water. The toxicity of soluble Al forms depends upon the delivered dose of Al+ 3 to target tissues. Trivalent Al reacts with water to produce bidentate superoxide coordination spheres [Al(O2)(H2O4)+ 2 and Al(H2O)6 + 3] that after complexation with O2•−, generate Al superoxides [Al(O2•)](H2O5)]+ 2. Semireduced AlO2• radicals deplete mitochondrial Fe and promote generation of H2O2, O2 • − and OH•. Thus, it is the Al+ 3-induced formation of oxygen radicals that accounts for the oxidative damage that leads to intrinsic apoptosis. In contrast, the toxicity of the insoluble Al oxides depends primarily on their behavior as particulates. Aluminum has been held responsible for human morbidity and mortality, but there is no consistent and convincing evidence to associate the Al found in food and drinking water at the doses and chemical forms presently consumed by people living in North America and Western Europe with increased risk for Alzheimer\u27s disease (AD). Neither is there clear evidence to show use of Al-containing underarm antiperspirants or cosmetics increases the risk of AD or breast cancer. Metallic Al, its oxides, and common Al salts have not been shown to be either genotoxic or carcinogenic. Aluminum exposures during neonatal and pediatric parenteral nutrition (PN) can impair bone mineralization and delay neurological development. Adverse effects to vaccines with Al adjuvants have occurred; however, recent controlled trials found that the immunologic response to certain vaccines with Al adjuvants was no greater, and in some cases less than, that after identical vaccination without Al adjuvants. The scientific literature on the adverse health effects of Al is extensive. Health risk assessments for Al must take into account individual co-factors (e.g., age, renal function, diet, gastric pH). Conclusions from the current review point to the need for refinement of the PTWI, reduction of Al contamination in PN solutions, justification for routine addition of Al to vaccines, and harmonization of OELs for Al substances

Germline selection shapes human mitochondrial DNA diversity.

Approximately 2.4% of the human mitochondrial DNA (mtDNA) genome exhibits common homoplasmic genetic variation. We analyzed 12,975 whole-genome sequences to show that 45.1% of individuals from 1526 mother-offspring pairs harbor a mixed population of mtDNA (heteroplasmy), but the propensity for maternal transmission differs across the mitochondrial genome. Over one generation, we observed selection both for and against variants in specific genomic regions; known variants were more likely to be transmitted than previously unknown variants. However, new heteroplasmies were more likely to match the nuclear genetic ancestry as opposed to the ancestry of the mitochondrial genome on which the mutations occurred, validating our findings in 40,325 individuals. Thus, human mtDNA at the population level is shaped by selective forces within the female germ line under nuclear genetic control, which ensures consistency between the two independent genetic lineages.NIHR, Wellcome Trust, MRC, Genomics Englan

Dating Quaternary lacustrine sediments in the McMurdo Dry Valleys, Antarctica

Reports of erroneously old 14C dates for modern Antarctic materials have thrown doubt into 14C chronologies. The carbon reservoir effect purported to exist in Quaternary lacustrine sediments of the McMurdo Dry Valleys was investigated by studying 14C distribution, and testing alternate dating techniques. Our results show that the carbon reservoir effect is not pervasive. Stream and near-shore microbial mats and dissolved inorganic carbon (DIC) in the surface waters of Lake Fryxell are in equilibrium with modern 14CO2. The surface waters of Lake Hoare and Lake Bonney, however, have DIC14C ages of 1650 and 2080 yr B.P., respectively. These older age estimates are suggested to be due to the direct input of large amounts of glacial melt with relict DIC. On the other hand, Lake Fryxell receives only a minor component of its inflow directly from a glacier, while a large component must travel long distances in numerous shallow ephemeral streams after leaving local valley glaciers. This mode of melt-water input allows the water to equilibrate with modern CO2 before entering the lake. Bottom-water14C ages for Lake Hoare closely match surface sediment ages, supporting the widely published period ∼1200 yr B.P. (after a 1650 yr reservoir correction) when most dry valley lakes apparently evaporated to small brine ponds and/or disappeared completely. Lake Bonney bottom-water is ∼8000 yr B.P. Carbon dating is shown to be a viable technique for lake edge deposits, and possibly lake bottom deposits where a correction to the sediment surface age can be obtained. However, we conclude that deep-water paleolake deposits can not be reliably dated using 14C alone because of an inability to determine the age of the reservoir correction (i.e. accounting for the initial carbon reservoir, plus the age of the bottom water). A suite of alternative and complimentary dating techniques were tested on modern and late Holocene lacustrine deposits. These include thermoluminescence (TL), 210Pb, 137Cs, and paleomagnetism. Of these techniques, TL (or the more sensitive optically stimulated luminescence) holds the most promise for correcting lake sediment 14C ages. TL dating of the modern Lake Hoare sediments showed an ∼1000 yr relict signal. This signal is unaffected by the age of the lake water. Both 210Pb and 137Cs occurred in very low levels in the sediments and do not appear to be viable dating techniques for the perennially ice-covered lakes of the McMurdo Dry Valleys. Paleomagnetism was not suited to the coarse-grained nature of Lake Hoare bottom sediments, but could be a useful alternative given a finer sediment type

Quantification of JCV DNA and VP1 capsid protein.

<p>Log-log plot of the concentration of JCV genomes vs capsids per microliter tissue for all 9 PML blocks (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0155897#pone.0155897.t002" target="_blank">Table 2</a>). Data are means ± standard deviation based on triplicate measurements. Blue dashed line indicates theoretical relationship for one capsid per viral genome. Linear regression reveals a positive relationship with <i>r</i>² = 0.96, <i>p</i><0.0001 (regression line not shown). Asterisk designates the uncertainty of the protein measurement of VP1 in NL3, which was below the assay’s lower limit of quantitation (not shown).</p

VP1 colocalizes with nearby myelin in recently lysed cells.

<p>Representative 5-μm confocal Z-axis stack images of regions of recent virus-induced cell lysis in otherwise intact white matter (A, B, D, E) or gray matter (C) of human PML, or white matter in an SIV-positive rhesus macaque with SV40 PML-like CNS disease (F), stained for VP1 (green in A-F, A″–F″, and A*-F*) and MBP (red in A-C, F, A′–C′, F′, and A*-C*, F*), GFAP (red in D, D′, D*), or IBA1 (red in E, E′, E*). A*–F* show representative colocalization (coloc) images from a single image from each Z-stack, with magenta pixels designating VP1/MBP colocalization (magenta arrows); Image quantitation of these representative Z-stack images revealed 89% and 86% of VP1 colocalized with MBP in white matter (A* and B*, respectively); 34% colocalized in gray matter (C*); and 13% and 2.9% of VP1 colocalized with GFAP or IBA1 (D* and E*, respectively). In SV40 PML-like disease (F), a subset of VP1 was dispersed in a linear pattern and colocalized with partially demyelinated MBP-positive axons (magenta arrow in F*). Occasional cytoplasmic VP1 viral aggregates were seen in cytoplasm of microglia (green arrow in E) or astrocytes, but nuclei of these cell types did not show VP1 positivity that would be indicative of productive infection. Scale bar in panel A = 10 μm.</p