Reform of the Swiss Fiscal Equalisation System

Finanzausgleich, Reform, Schweiz, Fiscal transfer, Reform, Switzerland

First-principles investigation of spin polarized conductance in atomic carbon wire

We analyze spin-dependent energetics and conductance for one dimensional (1D) atomic carbon wires consisting of terminal magnetic (Co) and interior nonmagnetic (C) atoms sandwiched between gold electrodes, obtained employing first-principles gradient corrected density functional theory and Landauer's formalism for conductance. Wires containing an even number of interior carbon atoms are found to be acetylenic with sigma-pi bonding patterns, while cumulene structures are seen in wires containing odd number of interior carbon atoms, as a result of strong pi-conjugation. Ground states of carbon wires containing up to 13 C atoms are found to have anti-parallel spin configurations of the two terminal Co atoms, while the 14 C wire has a parallel Co spin configuration in the ground state. The stability of the anti-ferromagnetic state in the wires is ascribed to a super-exchange effect. For the cumulenic wires this effect is constant for all wire lengths. For the acetylenic wires, the super-exchange effect diminishes as the wire length increases, going to zero for the atomic wire containing 14 carbon atoms. Conductance calculations at the zero bias limit show spin-valve behavior, with the parallel Co spin configuration state giving higher conductance than the corresponding anti-parallel state, and a non-monotonic variation of conductance with the length of the wires for both spin configurations.Comment: revtex, 6 pages, 5 figure

Midlatitude atmospheric circulation responses under 1.5C and 2.0C warming and implications for regional impacts

This study investigates the global response of the midlatitude atmospheric circulation to 1.5◦C and 5 2.0◦C of warming using the HAPPI “Half a degree Additional warming, Prognosis and Projected Im- pacts” ensemble, with a focus on the winter season. Characterizing and understanding this response is critical for accurately assessing the near-term regional impacts of climate change and the benefits of limiting warming to 1.5◦C above pre-industrial levels, as advocated by the Paris Agreement of the United Nations Framework Convention on Climate Change (UNFCCC). The HAPPI experimental 10 design allows an assessment of uncertainty in the circulation response due to model dependence and internal variability. Internal variability is found to dominate the multi-model mean response of the jet streams, storm tracks and stationary waves across most of the midlatitudes; larger signals in these features are mostly consistent with those seen in more strongly forced warming scenarios. Signals that emerge in the 1.5◦C experiment are a weakening of storm activity over North America, an inland 15 shift of the North American stationary ridge, an equatorward shift of the North Pacific jet exit, and an equatorward intensification of the South Pacific jet. Signals that emerge under an additional 0.5◦C of warming include a poleward shift of the North Atlantic jet exit, an eastward extension of the North Atlantic storm track, and an intensification on the flanks of the Southern Hemisphere storm track. Case studies explore the implications of these circulation responses for precipitation impacts in the 20 Mediterranean, western Europe and on the North American west coast, paying particular attention to possible outcomes at the tails of the response distributions. For example, the projected weakening of the Mediterranean storm track emerges in the 2◦C warmer world, with exceptionally dry decades becoming five times more likely

Life history evolution, species differences and phenotypic plasticity in hemiparasitic eyebrights (Euphrasia)

Premise of the study: Species delimitation in parasitic organisms is challenging as traits used in the identification of species are often plastic and vary depending on the host. Here, we use species from a recent radiation of generalist hemiparasitic Euphrasia to investigate trait variation and trait plasticity. We test whether Euphrasia species show reliable trait differences, investigate whether these differences correspond to life history trade-offs between growth and reproduction, and quantify plasticity in response to host species. Methods: We perform common garden experiments to evaluate trait differences between eleven Euphrasia taxa grown on a common host, document phenotypic plasticity when a single Euphrasia species is grown on eight different hosts, and relate our observations to trait differences recorded in the wild. Key results: Euphrasia exhibit variation in life history strategies; some individuals transition rapidly to flower at the expense of early season growth, while others invest in vegetative growth and delay flowering. Life history differences are present between some species, though many related taxa lack clear-cut trait differences. Species differences are further blurred by phenotypic plasticity—many traits are plastic and change with host type or between environments. Conclusions: Phenotypic plasticity in response to host and environment confounds species delimitation in Euphrasia. When grown in a common garden environment it is possible to identify some morphologically distinct taxa, though others represent morphologically similar shallow segregates. Trait differences present between some species and populations demonstrates the rapid evolution of distinct life history strategies in response to local ecological conditions."Manyhosts.csv" contains morphological measurements from one Euphrasia arctica population from North Berwick, Scotland, grown with eight hosts. "Manyspecies.csv" contains morphological measurements of five Euphrasia species and six natural Euphrasia hybrids grown on a single host, Trifolium repens. "Earlylate.csv" contains repeated growth measurements at different times of year, used in correlations of height at end of season. "Wildcommon.csv" contains Euphrasia species grown in the common garden experiment and wild collected plants for trait comparisons.Data collection is detailed in the associated manuscript. Post collection data processing can be viewed at: https://github.com/Euphrasiologist/phenotypic_plasticity_euphrasi

High expression of Cathepsin E in tissues but not blood of patients with Barrett’s esophagus and adenocarcinoma

Background Cathepsin E (CTSE), an aspartic proteinase, is differentially expressed in the metaplasia–dysplasia–neoplasia sequence of gastric and colon cancer. We evaluated CTSE in Barrett’s esophagus (BE) and cancer because increased CTSE levels are linked to improved survival in several cancers, and other cathepsins are up-regulated in BE and esophageal adenocarcinoma (EAC). Methods A total of 273 pretreatment tissues from 199 patients were analyzed [31 normal squamous esophagus (NE), 29 BE intestinal metaplasia, 31 BE with dysplasia (BE/D), 108 EAC]. CTSE relative mRNA expression was measured by real-time polymerase chain reaction, and protein expression was measured by immunohistochemistry. CTSE serum levels were determined by enzyme-linked immunosorbent assay. Results Median CTSE mRNA expression levels were ≥1,000-fold higher in BE/intestinal metaplasia and BE/D compared to NE. CTSE levels were significantly lower in EAC compared to BE/intestinal metaplasia and BE/D, but significantly higher than NE levels. A similar expression pattern was present in immunohistochemistry, with absent staining in NE, intense staining in intestinal metaplasia and dysplasia, and less intense EAC staining. CTSE serum analysis did not discriminate patient groups. In a uni- and multivariable Cox proportional hazards model, CTSE expression was not significantly associated with survival in patients with EAC, although CTSE expression above the 25th percentile was associated with a 41 % relative risk reduction for death (hazard ratio 0.59, 95 % confidence interval 0.27–1.26, p = 0.17). Conclusions CTSE mRNA expression is up-regulated more than any known gene in Barrett intestinal metaplasia and dysplasia tissues. Protein expression is similarly highly intense in intestinal metaplasia and dysplasia tissues

CRISPR-Cas adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonized by 5' ssDNA exonucleases

© The Author(s) 2018. Prokaryotic adaptive immunity is established against mobile genetic elements (MGEs) by 'naïve adaptation' when DNA fragments from a newly encountered MGE are integrated into CRISPR-Cas systems. In Escherichia coli, DNA integration catalyzed by Cas1- Cas2 integrase is well understood in mechanistic and structural detail butmuch less is known about events prior to integration that generate DNA for capture by Cas1-Cas2. Naïve adaptation in E. coli is thought to depend on the DNA helicase-nuclease RecBCD for generating DNA fragments for capture by Cas1- Cas2. The genetics presented here show that naïve adaptation does not require RecBCD nuclease activity but that helicase activity may be important. RecA loading by RecBCD inhibits adaptation explaining previously observed adaptation phenotypes that implicated RecBCD nuclease activity. Genetic analysis of other E. coli nucleases and naïve adaptation revealed that 5' ssDNA tailed DNA molecules promote new spacer acquisition. We show that purified E. coli Cas1-Cas2 complex binds to and nicks 5' ssDNA tailed duplexes and propose that E. coli Cas1-Cas2 nuclease activity on such DNA structures supports naïve adaptation

The compositional and evolutionary logic of metabolism

Metabolism displays striking and robust regularities in the forms of modularity and hierarchy, whose composition may be compactly described. This renders metabolic architecture comprehensible as a system, and suggests the order in which layers of that system emerged. Metabolism also serves as the foundation in other hierarchies, at least up to cellular integration including bioenergetics and molecular replication, and trophic ecology. The recapitulation of patterns first seen in metabolism, in these higher levels, suggests metabolism as a source of causation or constraint on many forms of organization in the biosphere. We identify as modules widely reused subsets of chemicals, reactions, or functions, each with a conserved internal structure. At the small molecule substrate level, module boundaries are generally associated with the most complex reaction mechanisms and the most conserved enzymes. Cofactors form a structurally and functionally distinctive control layer over the small-molecule substrate. Complex cofactors are often used at module boundaries of the substrate level, while simpler ones participate in widely used reactions. Cofactor functions thus act as "keys" that incorporate classes of organic reactions within biochemistry. The same modules that organize the compositional diversity of metabolism are argued to have governed long-term evolution. Early evolution of core metabolism, especially carbon-fixation, appears to have required few innovations among a small number of conserved modules, to produce adaptations to simple biogeochemical changes of environment. We demonstrate these features of metabolism at several levels of hierarchy, beginning with the small-molecule substrate and network architecture, continuing with cofactors and key conserved reactions, and culminating in the aggregation of multiple diverse physical and biochemical processes in cells.Comment: 56 pages, 28 figure