28,891 research outputs found
Kinematical and mechanical aspects of wafer slicing
Some recently achieved results concerning the technological fundamentals of slurry sawing are presented. The specific material removal process and the related kinematic and geometric contact conditions between workpiece and saw blade are described. The result of a functional description of the slurry sawing process is presented, expressing the main process criteria, such as infeed per stroke, specific removal rate, specific tool wear, and vertical stroke intensity, in terms of the dominating process parameters, such as stroke length, width of workpiece, stroke frequency, specific cutting force and slurry specification
Characterization of monoclonal and polyclonal antibodies to bovine enteric coronavirus: Establishment of an efficient ELISA for antigen detection in feces
Monoclonal antibodies to bovine enteric coronavirus (BEC) were produced. Additionally, polyclonal antibodies were made in rabbits and guinea pigs and extracted from the yolk of immunized hens. The antibodies were characterized by neutralization test, hemagglutination inhibition test, enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Neutralizing antibody titers of polyclonal antisera ranged from 1:1280 to 1:40 000. Only one out of 908 hybridoma colonies tested secreted antibodies with neutralizing activity. By ELISA, polyclonal sera exhibited high background reactions that could be significantly reduced by treatment with kaolin in the case of rabbit sera.
Attempts to establish an ELISA for BEC antigen detection based on polyclonal sera failed due to low sensitivity and specificity. Optimal results were achieved when a mixture of two monoclonal antibodies was coated onto microplates for antigen capture, while rabbit hyperimmune serum served as detecting antibodies in an indirect assay.
The combination of the two monoclonal antibodies did not increase sensitivity synergistically, but in a compensatory fashion, probably because of epitope differences between BEC field strain
Abstract Ces\`aro spaces: Integral representations
The Ces\`aro function spaces , , have
received renewed attention in recent years. Many properties of are
known. Less is known about when the Ces\`aro operator takes its values
in a rearrangement invariant (r.i.) space other than . In this paper
we study the spaces via the methods of vector measures and vector
integration. These techniques allow us to identify the absolutely continuous
part of and the Fatou completion of ; to show that is
never reflexive and never r.i.; to identify when is weakly sequentially
complete, when it is isomorphic to an AL-space, and when it has the
Dunford-Pettis property. The same techniques are used to analyze the operator
; it is never compact but, it can be completely continuous.Comment: 21 page
Density Functional Theory of the Hubbard-Holstein Model
We present a density functional theory (DFT) for lattice models with local
electron-electron (e-e) and electron-phonon (e-ph) interactions.
Exchange-correlation potentials are derived via dynamical mean field theory for
the infinite-dimensional Bethe lattice, and analytically for an isolated
Hubbard-Holstein site. These potentials exhibit discontinuities as a function
of the density, which depend on the relative strength of the e-e and e-ph
interactions. By comparing to exact benchmarks, we show that the DFT formalism
gives a good description of the linear conductance and real-time dynamics.Comment: 5 pages, 3 figures, supplemental material provided as pd
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