Rapid and high capacity methane storage in clathrate hydrates using surfactant dry solution

Surfactant dry solution (DS) was prepared by mixing sodium dodecyl sulfate (SDS) solution, hydrophobic silica nanoparticles and air in a high speed blender. Flour-like SDS-DS combines the advantages of dispersed dry water and active SDS solution. Methane storage in clathrate hydrates using SDS-DS was investigated in a stainless steel vessel without stirring under the condition of 5.0MPa and 273.2K. The results demonstrated that highly dispersed SDS-DS could significantly enhance formation kinetics and storage capacity of methane hydrate. SDS-DS exhibited about the same methane storage capacity (172.96m3 m-3) as dry water, but faster storage rates than dry water. Compared to SDS solution, SDS-DS had similar storage rates (7.44m3 m-3 min-1) and higher methane storage capacity under the relative low pressure. However, the aggregation of partial SDS-DS powders destroyed its original dispersive property after hydrate dissociation

Eriodictyol modulates glioma cell autophagy and apoptosis by inhibition of PI3K/Akt/mTOR signaling pathway

Purpose: To investigate the effects of eriodictyol (ERD) on U251 human glioma cell cycle and viability, autophagy and apoptosis by modulation of PI3/Akt/mTOR signaling cascade. Methods: 740 Y-P was used to activate U251 human glioma cells. For exploring ERD effects, the U251 cells were treated with ERD and 740 Y-P together. MTT assay was used to elucidate cell viability and apoptosis. The expression of autophagic proteins (LC3B and Beclin-1), and apoptotic proteins (Bcl-2 and Bax) were quantified using Western blotting. To explore the role of PI3K/Akt/mTOR signaling pathway, their expression was measured in comparison to their respective phosphorylated derivatives by Western blotting. Results: ERD exposure downregulated p-PI3K and p-Akt protein expression. The results also indicate that ERD reduced cell viability and stimulated apoptosis in U251 cells (p < 0.05). Consequently, Bax expression was upregulated and the expression of Bcl-2 was downregulated. ERD enhanced the autophagy of glioma cells U251 by enhancing LC3B and Beclin-1 expression (p < 0.05). These effects were opposite to that revealed by 740 Y-P exposure alone. Conclusion: ERD reduces U251 human glioma cell viability, and triggers cell autophagy and apoptosis, which is significantly correlated to downregulation of PI3K/Akt/mTOR signalling cascade. Thus, the compound can potentially be used for the treatment of glioma

Universal Global Imprints of Genome Growth and Evolution – Equivalent Length and Cumulative Mutation Density

BACKGROUND: Segmental duplication is widely held to be an important mode of genome growth and evolution. Yet how this would affect the global structure of genomes has been little discussed. METHODS/PRINCIPAL FINDINGS: Here, we show that equivalent length, or L(e), a quantity determined by the variance of fluctuating part of the distribution of the k-mer frequencies in a genome, characterizes the latter's global structure. We computed the L(e)s of 865 complete chromosomes and found that they have nearly universal but (k-dependent) values. The differences among the L(e) of a chromosome and those of its coding and non-coding parts were found to be slight. CONCLUSIONS: We verified that these non-trivial results are natural consequences of a genome growth model characterized by random segmental duplication and random point mutation, but not of any model whose dominant growth mechanism is not segmental duplication. Our study also indicates that genomes have a nearly universal cumulative "point" mutation density of about 0.73 mutations per site that is compatible with the relatively low mutation rates of (1-5) x 10(-3)/site/Mya previously determined by sequence comparison for the human and E. coli genomes

Inverse Symmetry in Complete Genomes and Whole-Genome Inverse Duplication

The cause of symmetry is usually subtle, and its study often leads to a deeper understanding of the bearer of the symmetry. To gain insight into the dynamics driving the growth and evolution of genomes, we conducted a comprehensive study of textual symmetries in 786 complete chromosomes. We focused on symmetry based on our belief that, in spite of their extreme diversity, genomes must share common dynamical principles and mechanisms that drive their growth and evolution, and that the most robust footprints of such dynamics are symmetry related. We found that while complement and reverse symmetries are essentially absent in genomic sequences, inverse–complement plus reverse–symmetry is prevalent in complex patterns in most chromosomes, a vast majority of which have near maximum global inverse symmetry. We also discovered relations that can quantitatively account for the long observed but unexplained phenomenon of -mer skews in genomes. Our results suggest segmental and whole-genome inverse duplications are important mechanisms in genome growth and evolution, probably because they are efficient means by which the genome can exploit its double-stranded structure to enrich its code-inventory

Revisiting the Long/Soft-Short/Hard Classification of Gamma-Ray Bursts in the Fermi Era

We perform a statistical analysis of the temporal and spectral properties of the latest Fermi gamma-ray bursts (GRBs) to revisit the classification of GRBs. We find that the bimodalities of duration and the energy ratio ($E_{\mathrm{peak}}$/Fluence) and the anti-correlation between spectral hardness (hardness ratio ($HR$), peak energy and spectral index) and duration ($T_{90}$) support the long/soft $-$ short/hard classification scheme for Fermi GRBs. The $HR - T_{90}$ anti-correlation strongly depends upon the spectral shape of GRBs and energy bands, and the bursts with the curved spectra in the typical BATSE energy bands show a tighter anti-correlation than those with the power-law spectra in the typical BAT energy bands. This might explain why the $HR - T_{90}$ correlation is not evident for those GRB samples detected by instruments like {\it Swift} with a narrower/softer energy bandpass. We also analyze the intrinsic energy correlation for the GRBs with measured redshifts and well defined peak energies. The current sample suggests $E_{\mathrm{p,rest}}=2455\times (E_{\mathrm{iso}}/10^{52})^{0.59}$ for short GRBs, significantly different from that for long GRBs. However, both the long and short GRBs comply with the same $E_{\mathrm{p,rest}}-L_{\mathrm{iso}}$ correlation.Comment: 17 pages, 9 figures, 4 tables. Accepted for publication in Ap

Transcriptomic analyses of regenerating adult feathers in chicken

Transcriptome Expression Data. Table of mapped reads to Galgal4 transcripts for all 15 data sets. FPKM (Fragments per kilobase of exon per million fragments mapped): normalized transcript abundance values for each gene in the indicated tissues. (CSV 1314 kb