Creation of a Vector for Regulated Expression of Antisense RNA in Escherichia Coli

A plasmid (pGEM-SD) was constructed in which the Shine-Dalgarno sequence had been deleted from pGEM-7zf(+) plasmid to generate a vector for regulated expression of antisense RNA. The binding of antisense RNA to mRNA provides a potent mechanism by which specific transcripts can be translationally inactivated. Although part of the lac operator sequence was deleted in pGEM-SD plasmid, it was proven that mRNA still can be induced under the control of lac promoter. Recombinant plasmids were generated by ligating bacterial genornic DNA into pGEM-SD plasmid, but the orientation of the inserted gene with respect to the lac promoter has not been determined. These preliminary results show that gene expression can be turned on and off via the pGEM-SD vector. Future experiments will screen antisense recombinant clones for those which would inhibit specific gene products by plating on different selective indicator media both with and without the inducer IPTG. This will allow investigation of the biological functions associated with genomic DNA sequences by means of gene mapping with antisense RNA

Creation of a Vector for Regulated Expression of Antisense RNA in Escherichia Coli

A plasmid (pGEM-SD) was constructed in which the Shine-Dalgarno sequence had been deleted from pGEM-7zf(+) plasmid to generate a vector for regulated expression of antisense RNA. The binding of antisense RNA to mRNA provides a potent mechanism by which specific transcripts can be translationally inactivated. Although part of the lac operator sequence was deleted in pGEM-SD plasmid, it was proven that mRNA still can be induced under the control of lac promoter. Recombinant plasmids were generated by ligating bacterial genornic DNA into pGEM-SD plasmid, but the orientation of the inserted gene with respect to the lac promoter has not been determined. These preliminary results show that gene expression can be turned on and off via the pGEM-SD vector. Future experiments will screen antisense recombinant clones for those which would inhibit specific gene products by plating on different selective indicator media both with and without the inducer IPTG. This will allow investigation of the biological functions associated with genomic DNA sequences by means of gene mapping with antisense RNA

DEFINITION AND CONSTRUCTION OF THE CORE COMPETENCE IN REAL ESTATE INDUSTRY

The real estate is a very high risk profession. Its funds devotion is big, the operation period is long and the market change quickly. At the same time, the real estate has to face the high level competition that brought by international capital investment. The key to deal with these problems is how to set up and enhance the core competence of the real estate industry while integrating the strategic resources. In this paper we will analyze the features of core competence, thus define the core competence in real estate Industry. The main competition factors of real estate industry include land resources, capital scale and market management. Accordingly, we put forward the solution to establish the core competence of real estate industry. Key words: Core competence, Real estate, Investmen

How special is special interest tourism – and how special are special interest tourists? A perspective article in a Chinese context

Special interest tourism has emerged as a valuable niche market for tourism destinations in the past decade. However, tourism scholars have generally struggled to answer McKercher and Chan’s [2005. How special is special interest tourism? Journal of Travel Research, 44(1), 21–31] question, ‘How special is special interest tourism?’ Such ambiguity extends to the related enquiry, ‘How special are special interest tourists?’, in attempting to define special interest tourists. This perspective research letter discusses these questions in terms of Chinese outbound tourism. Based upon the reflection of previous research, the authors’ thoughts and ongoing research in this area, knowledge gaps are identified and research directions for scholars who are also interested special interest tourism are offered