The HIV-1 Protein Vpr Targets the Endoribonuclease Dicer for Proteasomal Degradation to Boost Macrophage Infection

The HIV-1 protein Vpr enhances macrophage infection, triggers G2 cell cycle arrest, and targets cells for NK-cell killing. Vpr acts through the CRL4DCAF1 ubiquitin ligase complex to cause G2 arrest and trigger expression of NK ligands. Corresponding ubiquitination targets have not been identified. UNG2 and SMUG1 are the only known substrates for Vpr-directed depletion through CRL4DCAF1. Here we identify the endoribonuclease Dicer as a target of HIV-1 Vpr-directed proteasomal degradation through CRL4DCAF1. We show that HIV-1 Vpr inhibits short hairpin RNA function as expected upon reduction of Dicer levels. Dicer inhibits HIV-1 replication in T cells. We demonstrate that Dicer also restricts HIV-1 replication in human monocyte-derived macrophages (MDM) and that reducing Dicer expression in MDMs enhances HIV-1 infection in a Vpr-dependent manner. Our results support a model in which Vpr complexes with human Dicer to boost its interaction with the CRL4DCAF1 ubiquitin ligase complex and its subsequent degradation

Numerical study of pattern formation following a convective instability in non-Boussinesq fluids

We present a numerical study of a model of pattern formation following a convective instability in a non-Boussinesq fluid. It is shown that many of the features observed in convection experiments conducted on $CO_{2}$ gas can be reproduced by using a generalized two-dimensional Swift-Hohenberg equation. The formation of hexagonal patterns, rolls and spirals is studied, as well as the transitions and competition among them. We also study nucleation and growth of hexagonal patterns and find that the front velocity in this two dimensional model is consistent with the prediction of marginal stability theory for one dimensional fronts.Comment: 9 pages, report FSU-SCRI-92-6

Can Short-Range Interactions Mediate a Bose Metal Phase in 2D?

We show here based on a 1-loop scaling analysis that short-range interactions are strongly irrelevant perturbations near the insulator-superconductor (IST) quantum critical point. The lack of any proof that short-range interactions mediate physics which is present only in strong coupling leads us to conclude that short-range interactions are strictly irrelevant near the IST quantum critical point. Hence, we argue that no new physics, such as the formation of a uniform Bose metal phase can arise from an interplay between on-site and nearest-neighbour interactions.Comment: 3 pages, 1 .eps file. SUbmitted to Phys. Rev.

Self Duality and Oblique Confinement in Planar Gauge Theories

We investigate the non-perturbative structure of two planar $Z_p \times Z_p$ lattice gauge models and discuss their relevance to two-dimensional condensed matter systems and Josephson junction arrays. Both models involve two compact U(1) gauge fields with Chern-Simons interactions, which break the symmetry down to $Z_p \times Z_p$. By identifying the relevant topological excitations (instantons) and their interactions we determine the phase structure of the models. Our results match observed quantum phase transitions in Josephson junction arrays and suggest also the possibility of {\it oblique confining ground states} corresponding to quantum Hall regimes for either charges or vortices.Comment: 32 pages, harvma

A new displacement-based approach to calculate stress intensity factors with the boundary element method

The analysis of cracked brittle mechanical components considering linear elastic fracture mechanics is usually reduced to the evaluation of stress intensity factors (SIFs). The SIF calculation can be carried out experimentally, theoretically or numerically. Each methodology has its own advantages but the use of numerical methods has be-come very popular. Several schemes for numerical SIF calculations have been developed, the J-integral method being one of the most widely used because of its energy-like formulation. Additionally, some variations of the J-integral method, such as displacement-based methods, are also becoming popular due to their simplicity. In this work, a simple displacement-based scheme is proposed to calculate SIFs, and its performance is compared with contour integrals. These schemes are all implemented with the Boundary Element Method (BEM) in order to exploit its advantages in crack growth modelling. Some simple examples are solved with the BEM and the calculated SIF values are compared against available solutions, showing good agreement between the different schemes

Blow up criterion for compressible nematic liquid crystal flows in dimension three

In this paper, we consider the short time strong solution to a simplified hydrodynamic flow modeling the compressible, nematic liquid crystal materials in dimension three. We establish a criterion for possible breakdown of such solutions at finite time in terms of the temporal integral of both the maximum norm of the deformation tensor of velocity gradient and the square of maximum norm of gradient of liquid crystal director field.Comment: 22 page

Spin Ordering and Quasiparticles in Spin Triplet Superconducting Liquids

Spin ordering and its effect on low energy quasiparticles in a p-wave superconducting liquid are investigated. We show that there is a new 2D p-wave superconducting liquid where the ground state is rotation invariant. In quantum spin disordered liquids, the low energy quasiparticles are bound states of the bare Bogolubov- De Gennes ({\em BdeG}) quasiparticles and zero energy skyrmions, which are charge neutral bosons at the low energy limit. Further more, spin collective excitations are fractionalized ones carrying a half spin and obeying fermionic statistics. In thermally spin disordered limits, the quasi-particles are bound states of bare {\em BdeG} quasi-particles. The latter situation can be realized in some layered p-wave superconductors where the spin-orbit coupling is weak.Comment: 5 pages, no figures; published versio

Nondissipative Drag Conductance as a Topological Quantum Number

We show in this paper that the boundary condition averaged nondissipative drag conductance of two coupled mesoscopic rings with no tunneling, evaluated in a particular many-particle eigenstate, is a topological invariant characterized by a Chern integer. Physical implications of this observation are discussed.Comment: 4 pages, no figure. Title modified and significant revision made to the text. Final version appeared in PR

Well-Posedness of Nematic Liquid Crystal Flow in Luloc3(R3)L^3_{\hbox{uloc}}(\R^3)

In this paper, we establish the local well-posedness for the Cauchy problem of the simplified version of hydrodynamic flow of nematic liquid crystals (\ref{LLF}) in $\mathbb R^3$ for any initial data $(u_0,d_0)$ having small $L^3_{\hbox{uloc}}$-norm of $(u_0,\nabla d_0)$. Here $L^3_{\hbox{uloc}}(\mathbb R^3)$ is the space of uniformly locally $L^3$-integrable functions. For any initial data $(u_0, d_0)$ with small $\displaystyle |(u_0,\nabla d_0)|_{L^3(\mathbb R^3)}$, we show that there exists a unique, global solution to (\ref{LLF}) which is smooth for $t>0$ and has monotone deceasing $L^3$-energy for $t\ge 0$.Comment: 29 page

A reduction of unilateral ureteral obstruction-induced renal fibrosis by a therapy combining valsartan with aliskiren

Wu WP, Chang CH, Chiu YT, Ku CL, Wen MC, Shu KH, Wu MJ. A reduction of unilateral ureteral obstruction-induced renal fibrosis by a therapy combining valsartan with aliskiren. Am J Physiol Renal Physiol 299: F929-F941, 2010. First published August 4, 2010; doi:10.1152/ajprenal.00192.2010.-The protective effect of combination therapy with valsartan and aliskiren against renal fibrosis remains to be defined. This study was undertaken to examine the protective effects of the combination of valsartan and aliskiren against renal fibrosis induced by unilateral ureteral obstruction (UUO). Combination therapy with valsartan (15 mg . kg(-1) . day(-1)) and aliskiren (10 mg . kg(-1) . day(-1)), valsartan monotherapy (30 mg . kg(-1) . day(-1)), and aliskiren monotherapy (20 mg . kg(-1) . day(-1)) all significantly ameliorated the increase in blood urea nitrogen and the degree of hydronephrosis determined by the increase in weight and length of the obstructed kidney. The dose titration study and blood pressure measurement confirmed that the combination therapy provided a greater benefit independent of the vasodilatory effect. There were no significant changes in serum levels of creatinine, sodium, and potassium in UUO rats and any treatment groups. Combination therapy also attenuated UUO-related increases in the scores of tubular dilatation, interstitial volume, interstitial collagen deposition, alpha-smooth muscle actin, the activation of ERK 1/2, the infiltration of monocytes/macrophages, the mRNA expression of snail-1, and transforming growth factor-beta 1 to a greater extent compared with aliskiren or valsartan used alone. The mRNA expression of renin and the (pro) renin receptor significantly increased after UUO. Combination therapy and monotherapy of valsartan and aliskiren had a comparable enhancing effect on the mRNA expression of renin, whereas all these treatments did not affect the expression of the (pro) renin receptor. In conclusion, a direct renin inhibitor in conjunction with an angiotensin II receptor blocker exerts increased renal protection against renal fibrosis and inflammation during obstruction over either agent alone