Mechanical strain induces growth of vascular smooth muscle cells via autocrine action of PDGF.

The effect of cyclic mechanical strain on growth of neonatal rat vascular smooth muscle (VSM) cells were examined. Cells were grown on silicone elastomer plates subjected to cyclic strain (60 cycle/min) by application of a vacuum under the plates. A 48 h exposure to mechanical strain increased the basal rate of thymidine incorporation by threefold and increased cell number by 40% compared with cells grown on stationary rubber plates. Strain also increased the rate of thymidine incorporation in response to alpha-thrombin (from 15- to 33-fold), but not to PDGF. As determined by thymidine autoradiography, strain alone induced a fourfold increase in labeled nuclei at the periphery of dishes, where strain is maximal, and a 2-3-fold increase at the center of dishes. Strain appeared to induce the production of an autocrine growth factor(s), since conditioned medium from cells subjected to strain induced a fourfold increase in DNA synthesis in control cells. Western blots of medium conditioned on the cells subjected to strain indicate that the cells secrete both AA and BB forms of PDGF in response to strain. Northern blots of total cell RNA from cells exposed to strain for 24 h show increased steady-state level of mRNA for PDGF-A. Lastly, polyclonal antibodies to the AA form of PDGF reduced by 75% the mitogenic effect of strain and polyclonal antibodies to AB-PDGF reduced mitogenicity by 50%. Antibodies to bFGF did not significantly reduce the strain-induced thymidine incorporation. Thus, the mechanism of strain-induced growth appears to involve the intermediary action of secreted PDGF

Partially reconfigurable TVWS transceiver for use in UK and US markets

With more and more countries opening up sections of unlicensed spectrum for use by TV White Space (TVWS) devices, the prospect of building a device capable of operating in more than one world region is appealing. The difficulty is that the locations of TVWS bands within the radio spectrum are not globally harmonised. With this problem in mind, the purpose of this paper is to present a TVWS transceiver design which is capable of being reconfigured to operate in both the UK and US spectrum. We present three different configurations: one covering the UK TVWS spectrum and the remaining two covering the various locations of the US TVWS bands

Inner Planetary System Gap Complexity is a Predictor of Outer Giant Planets

The connection between inner small planets and outer giant planets is crucial to our understanding of planet formation across a wide range of orbital separations. While Kepler provided a plethora of compact multi-planet systems at short separations ($\lesssim 1$ AU), relatively little is known about the occurrence of giant companions at larger separations and how they impact the architectures of the inner systems. Here, we use the catalog of systems from the Kepler Giant Planet Search (KGPS) to study how the architectures of the inner transiting planets correlate with the presence of outer giant planets. We find that for systems with at least three small transiting planets, the distribution of inner-system gap complexity ($\mathcal{C}$), a measure of the deviation from uniform spacings, appears to differ ($p \lesssim 0.02$) between those with an outer giant planet ($50 M_\oplus \leq M_p\sin{i} \leq 13 M_{\rm Jup}$) and those without any outer giants. All four inner systems (with 3+ transiting planets) with outer giant(s) have a higher gap complexity ($\mathcal{C} > 0.32$) than 79% (19/24) of the inner systems without any outer giants (median $\mathcal{C} \simeq 0.06$). This suggests that one can predict the occurrence of outer giant companions by selecting multi-transiting systems with highly irregular spacings. We do not find any correlation between outer giant occurrence and the size (similarity or ordering) patterns of the inner planets. The larger gap complexities of inner systems with an outer giant hints that massive external planets play an important role in the formation and/or disruption of the inner systems.Comment: Published in AJ. 16 pages, 6 figures, 1 tabl

Controlled Manipulation of Mode Splitting in an Optical Microcavity by Two Rayleigh Scatterers

We report controlled manipulation of mode splitting in an optical microresonator coupled to two nanoprobes. It is demonstrated that, by controlling the positions of the nanoprobes, the split modes can be tuned simultaneously or individually and experience crossing or anti-crossing in frequency and linewidth. A tunable transition between standing wave mode and travelling wave mode is also observed. Underlying physics is discussed by developing a two-scatterer model which can be extended to multiple scatterers. Observed rich dynamics and tunability of split modes in a single microresonator will find immediate applications in optical sensing, opto-mechanics, filters and will provide a platform to study strong light-matter interactions in two-mode cavities.Comment: 9 pages, 5 figures, 14 references. Major revision. Published version in Optics Expres

Heat transport by turbulent Rayleigh-B\'enard convection for $\Pra\ \simeq 0.8and and 3\times 10^{12} \alt \Ra\ \alt 10^{15}:Aspectratio: Aspect ratio \Gamma = 0.50$

We report experimental results for heat-transport measurements, in the form of the Nusselt number \Nu, by turbulent Rayleigh-B\'enard convection in a cylindrical sample of aspect ratio $\Gamma \equiv D/L = 0.50$ ($D = 1.12$ m is the diameter and $L = 2.24$ m the height). The measurements were made using sulfur hexafluoride at pressures up to 19 bars as the fluid. They are for the Rayleigh-number range 3\times 10^{12} \alt \Ra \alt 10^{15} and for Prandtl numbers \Pra\ between 0.79 and 0.86. For \Ra < \Ra^*_1 \simeq 1.4\times 10^{13} we find \Nu = N_0 \Ra^{\gamma_{eff}} with $\gamma_{eff} = 0.312 \pm 0.002$, consistent with classical turbulent Rayleigh-B\'enard convection in a system with laminar boundary layers below the top and above the bottom plate. For \Ra^*_1 < \Ra < \Ra^*_2 (with \Ra^*_2 \simeq 5\times 10^{14}) $\gamma_{eff}$ gradually increases up to $0.37\pm 0.01$. We argue that above \Ra^*_2 the system is in the ultimate state of convection where the boundary layers, both thermal and kinetic, are also turbulent. Several previous measurements for $\Gamma = 0.50$ are re-examined and compared with the present results.Comment: 44 pages, 18 figures, submitted to NJ

Achieving highly efficient gene transfer to the bladder by increasing the molecular weight of polymer-based nanoparticles

Short dwell-time and poor penetration of the bladder permeability barrier (BPB) are the main obstacles to intravesical treatments for bladder diseases, and is evidenced by the lack of such therapeutic options on the market. Herein, we demonstrate that by finely tuning the molecular weight of our cationic polymer mucoadhesive nanoparticles, we enhanced our gene transfer, leading to improved adherence and penetrance through the BPB in a safe and efficient manner. Specifically, increasing the polymer molecular weight from 45 kDa to 83 kDa enhanced luciferase plasmid transfer to the healthy murine bladder, leading to 1.35 ng/g luciferase protein expression in the urothelium and lamina propria regions. The relatively higher molecular weight polymer (83 kDa) did not induce morphologic changes or inflammatory responses in the bladder. This approach of altering polymer molecular weight for prolonging gene transfer residence time and deeper penetration through the BPB could be the basis for the design of future gene therapies for bladder diseases

Down-regulation of GP130 signaling sensitizes bladder cancer to cisplatin by impairing Ku70 DNA repair signaling and promoting apoptosis

Chemoresistance is one of the barriers for the development of bladder cancer treatments. Previously, we showed that glycoprotein-130 (GP130) is overexpressed in chemoresistant bladder cancer cells and that knocking down GP130 expression reduced cell viability. In our current work, we showed that down-regulation of GP130 sensitized bladder cancer cells to cisplatin-based chemotherapy by activating DNA repair signaling. We performed immunohistochemistry and demonstrated a positive correlation between the levels of Ku70, an initiator of canonical non-homologous end joining repair (c-NHEJ) and suppressor of apoptosis, and GP130 in human bladder cancer specimens. GP130 knockdown by SC144, a small molecule inhibitor, in combination with cisplatin, increased the number of DNA lesions, specifically DNA double-stranded breaks, with a subsequent increase in apoptosis and reduced cell viability. Furthermore, GP130 inhibition attenuated Ku70 expression in bladder and breast cancer cells as well as in transformed kidney cells. In addition, we fabricated a novel polymer-lipid hybrid delivery system to facilitate GP130 siRNA delivery that had a similar efficiency when compared with Lipofectamine, but induced less toxicity

Numerical renormalization group of vortex aggregation in 2D decaying turbulence: the role of three-body interactions

In this paper, we introduce a numerical renormalization group procedure which permits long-time simulations of vortex dynamics and coalescence in a 2D turbulent decaying fluid. The number of vortices decreases as $N\sim t^{-\xi}$, with $\xi\approx 1$ instead of the value $\xi=4/3$ predicted by a na\"{\i}ve kinetic theory. For short time, we find an effective exponent $\xi\approx 0.7$ consistent with previous simulations and experiments. We show that the mean square displacement of surviving vortices grows as $\sim t^{1+\xi/2}$. Introducing effective dynamics for two-body and three-body collisions, we justify that only the latter become relevant at small vortex area coverage. A kinetic theory consistent with this mechanism leads to $\xi=1$. We find that the theoretical relations between kinetic parameters are all in good agreement with experiments.Comment: 23 RevTex pages including 7 EPS figures. Submitted to Phys. Rev. E (Some typos corrected; see also cond-mat/9911032