66 research outputs found
The evolutionary dynamics of protein-protein interaction networks inferred from the reconstruction of ancient networks
Cellular functions are based on the complex interplay of proteins, therefore
the structure and dynamics of these protein-protein interaction (PPI) networks
are the key to the functional understanding of cells. In the last years,
large-scale PPI networks of several model organisms were investigated.
Methodological improvements now allow the analysis of PPI networks of multiple
organisms simultaneously as well as the direct modeling of ancestral networks.
This provides the opportunity to challenge existing assumptions on network
evolution. We utilized present-day PPI networks from integrated datasets of
seven model organisms and developed a theoretical and bioinformatic framework
for studying the evolutionary dynamics of PPI networks. A novel filtering
approach using percolation analysis was developed to remove low confidence
interactions based on topological constraints. We then reconstructed the
ancient PPI networks of different ancestors, for which the ancestral proteomes,
as well as the ancestral interactions, were inferred. Ancestral proteins were
reconstructed using orthologous groups on different evolutionary levels. A
stochastic approach, using the duplication-divergence model, was developed for
estimating the probabilities of ancient interactions from today's PPI networks.
The growth rates for nodes, edges, sizes and modularities of the networks
indicate multiplicative growth and are consistent with the results from
independent static analysis. Our results support the duplication-divergence
model of evolution and indicate fractality and multiplicative growth as general
properties of the PPI network structure and dynamics
Piphillin predicts metagenomic composition and dynamics from DADA2-corrected 16S rDNA sequences
Shotgun metagenomic sequencing reveals the potential in microbial communities. However, lower-cost 16S ribosomal RNA (rRNA) gene sequencing provides taxonomic, not functional, observations. To remedy this, we previously introduced Piphillin, a software package that predicts functional metagenomic content based on the frequency of detected 16S rRNA gene sequences corresponding to genomes in regularly updated, functionally annotated genome databases. Piphillin (and similar tools) have previously been evaluated on 16S rRNA data processed by the clustering of sequences into operational taxonomic units (OTUs). New techniques such as amplicon sequence variant error correction are in increased use, but it is unknown if these techniques perform better in metagenomic content prediction pipelines, or if they should be treated the same as OTU data in respect to optimal pipeline parameters
Biology of a widespread uncultivated archaeon that contributes to carbon fixation in the subsurface
Subsurface microbial life contributes significantly to biogeochemical cycling, yet it remains largely uncharacterized, especially its archaeal members. This 'microbial dark matter' has been explored by recent studies that were, however, mostly based on DNA sequence information only. Here, we use diverse techniques including ultrastuctural analyses to link genomics to biology for the SM1 Euryarchaeon lineage, an uncultivated group of subsurface archaea. Phylogenomic analyses reveal this lineage to belong to a widespread group of archaea that we propose to classify as a new euryarchaeal order ('Candidatus Altiarchaeales'). The representative, double-membraned species 'Candidatus Altiarchaeum hamiconexum' has an autotrophic metabolism that uses a not-yet-reported Factor(420)-free reductive acetyl-CoA pathway, confirmed by stable carbon isotopic measurements of archaeal lipids. Our results indicate that this lineage has evolved specific metabolic and structural features like nano-grappling hooks empowering this widely distributed archaeon to predominate anaerobic groundwater, where it may represent an important carbon dioxide sink
A viability-linked metagenomic analysis of cleanroom environments: eukarya, prokaryotes, and viruses.
Recombination drives evolution of the Clostridium difficile 16S-23S rRNA intergenic spacer region
PCR-ribotyping, a typing method based on size variation in 16S-23S rRNA intergenic spacer region (ISR), has been used widely for molecular epidemiological investigations of C. difficile infections. In the present study, we describe the sequence diversity of ISRs from 43 C. difficile strains, representing different PCR-ribotypes and suggest homologous recombination as a possible mechanism driving the evolution of 16S-23S rRNA ISRs. ISRs of 45 different lengths (ranging from 185 bp to 564 bp) were found among 458 ISRs. All ISRs could be described with one of the 22 different structural groups defined by the presence or absence of different sequence modulestRNAAla genes and different combinations of spacers of different lengths (33 bp, 53 bp or 20 bp) and 9 bp direct repeats separating the spacers. The ISR structural group, in most cases, coincided with the sequence length. ISRs that were of the same lengths had also very similar nucleotide sequence, suggesting that ISRs were not suitable for discriminating between different strains based only on the ISR sequence. Despite large variations in the length, the alignment of ISR sequences, based on the primary sequence and secondary structure information, revealed many conserved regions which were mainly involved in maturation of pre-rRNA. Phylogenetic analysis of the ISR alignment yielded strong evidence for intra- and inter-homologous recombination which could be one of the mechanisms driving the evolution of C. difficile 16S-23S ISRs. The modular structure of the ISR, the high sequence similarities of ISRs of the same sizes and the presence of homologous recombination also suggest that different copies of C. difficile 16S-23S rRNA ISR are evolving in concer
Complete Genome Sequence of Listeria monocytogenes Lm60, a Strain with an Enhanced Cold Adaptation Capacity
The complete genome sequence of Listeria monocytogenes Lm60, a fast cold-adapting serotype 1/2a human isolate, is presented.ISSN:2169-828
Input data overview.
<p>The numbers of proteins (nodes) and interactions extracted from STRING at each filter step before construction of the protein-protein interaction networks. Numbers are show on log-scale. (A) Number of nodes. (B) Number of interactions. Violet: STRING experimental score , green: conserved on all evolutionary levels, red: after filtering at , orange bars: after filtering at considering only largest (connected) component (LC); the largest component is necessary for the topological analysis.</p
Indication of homologous recombination in <i>C. difficile</i> 16S-23S rRNA intergenic spacer region.
<p>(A) Phylogenetic network constructed for 95 representative ISR sequences from 43 different <i>C. difficile</i> strains. (B) Phylogenetic network of 29 ISR sequences (279–282 bp) from 29 different strains of <i>C. difficile</i>. Only one ISR per strain was included in the analysis. Box-like branches seen on both figures indicate relative support for alternative relationships among ISRs, probably resulting from homologous recombination that was subsequently confirmed by statistical analysis. In groups I, II and III the ISRs without a gene for tRNA<sup>Ala</sup> are clustered and in group IV the ISRs with a tRNA gene.</p
Recombination Drives Evolution of the <i>Clostridium difficile</i> 16S-23S rRNA Intergenic Spacer Region
<div><p>PCR-ribotyping, a typing method based on size variation in 16S-23S rRNA intergenic spacer region (ISR), has been used widely for molecular epidemiological investigations of <i>C. difficile</i> infections. In the present study, we describe the sequence diversity of ISRs from 43 <i>C. difficile</i> strains, representing different PCR-ribotypes and suggest homologous recombination as a possible mechanism driving the evolution of 16S-23S rRNA ISRs. ISRs of 45 different lengths (ranging from 185 bp to 564 bp) were found among 458 ISRs. All ISRs could be described with one of the 22 different structural groups defined by the presence or absence of different sequence modules; tRNA<sup>Ala</sup> genes and different combinations of spacers of different lengths (33 bp, 53 bp or 20 bp) and 9 bp direct repeats separating the spacers. The ISR structural group, in most cases, coincided with the sequence length. ISRs that were of the same lengths had also very similar nucleotide sequence, suggesting that ISRs were not suitable for discriminating between different strains based only on the ISR sequence. Despite large variations in the length, the alignment of ISR sequences, based on the primary sequence and secondary structure information, revealed many conserved regions which were mainly involved in maturation of pre-rRNA. Phylogenetic analysis of the ISR alignment yielded strong evidence for intra- and inter-homologous recombination which could be one of the mechanisms driving the evolution of <i>C. difficile</i> 16S-23S ISRs. The modular structure of the ISR, the high sequence similarities of ISRs of the same sizes and the presence of homologous recombination also suggest that different copies of <i>C. difficile</i> 16S-23S rRNA ISR are evolving in concert.</p></div
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