A standardized G‐banded karyotype for the raccoon (Procyon lotor) compared with the domestic cat

We propose a standardized karyotype for the raccoon (Procyon lotor; 2n = 38, FN 74) and compare it with that of the domestic cat (2n = 38, FN 72). Numerous chromosomes (12) have similar and sometimes identical G-banding and 14 chromosome pairs have remained intact. Other chromosomes apparently differ by Robertsonian translocations and inversions. The conservation of these karyotypes is remarkable considering that the divergence of procyonids and felids predates 50 million years B.P. However, the common diploid number of 38 is not a primitive retention, as sometimes hypothesized. Instead, cats and raccoons converged on this chromosome number by a different series of translocations. The unsolved problems of carnivore phylogeny could benefit from a combination of high resolution banding and molecular cytogenetic analyses

On the realization of Symmetries in Quantum Mechanics

The aim of this paper is to give a simple, geometric proof of Wigner's theorem on the realization of symmetries in quantum mechanics that clarifies its relation to projective geometry. Although several proofs exist already, it seems that the relevance of Wigner's theorem is not fully appreciated in general. It is Wigner's theorem which allows the use of linear realizations of symmetries and therefore guarantees that, in the end, quantum theory stays a linear theory. In the present paper, we take a strictly geometrical point of view in order to prove this theorem. It becomes apparent that Wigner's theorem is nothing else but a corollary of the fundamental theorem of projective geometry. In this sense, the proof presented here is simple, transparent and therefore accessible even to elementary treatments in quantum mechanics.Comment: 8 page

Anomaly-matching and Higgs-less effective theories

We reconsider the low-energy effective theory for Higgs-less electroweak symmetry breaking: we study the anomaly-matching in the situation where all Goldstone fields disappear from the spectrum as a result of the Higgs mechanism. We find that the global SU(2)_L x SU(2)_R x U(1)_{B-L} symmetry of the underlying theory, which is spontaneously broken to SU(2)_{L+R} x U(1)_{B-L} has to be anomaly-free. For the sake of generality, we include the possibility of light spin-1/2 bound states resulting from the dynamics of the strongly-interacting symmetry-breaking sector, in addition to the Goldstone bosons. Such composite fermions may have non-standard couplings at the leading order, and an arbitrary total B-L charge. In order to perform the anomaly-matching in that case, we generalize the construction of the Wess-Zumino effective lagrangian. Composite fermions beyond the three known generations are theoretically allowed, and there are no restrictions from the anomaly-matching on their couplings nor on their U(1)_{B-L} charge. Absence of global anomalies for the composite sector as a whole does not preclude anomalous triple gauge boson couplings arising from composite fermion triangular diagrams. On the other hand, the trace of B-L over elementary fermions must vanish if all Goldstone modes are to disappear from the spectrum.Comment: Keywords: Anomalies in Field and String Theories, Spontaneous Symmetry Breaking, Beyond the Standard Model, Chiral Lagrangians. 33 pages, 7 figure

Interaction of the Tyrosine Kinase Pyk2 with the N -Methyl-d-aspartate Receptor Complex via the Src Homology 3 Domains of PSD-95 and SAP102

The protein-tyrosine kinase Pyk2/CAKbeta/CADTK is a key activator of Src in many cells. At hippocampal synapses, induction of long term potentiation requires the Pyk2/Src signaling pathway, which up-regulates the activity of N-methyl-d-aspartate-type glutamate receptors. Because localization of protein kinases close to their substrates is crucial for effective phosphorylation, we investigated how Pyk2 might be recruited to the N-methyl-d-aspartate receptor complex. This interaction is mediated by PSD-95 and its homolog SAP102. Both proteins colocalize with Pyk2 at postsynaptic dendritic spines in the cerebral cortex. The proline-rich regions in the C-terminal half of Pyk2 bind to the SH3 domain of PSD-95 and SAP102. The SH3 and guanylate kinase homology (GK) domain of PSD-95 and SAP102 interact intramolecularly, but the physiological significance of this interaction has been unclear. We show that Pyk2 effectively binds to the Src homology 3 (SH3) domain of SAP102 only when the GK domain is removed from the SH3 domain. Characterization of PSD-95 and SAP102 as adaptor proteins for Pyk2 fills a critical gap in the understanding of the spatial organization of the Pyk2-Src signaling pathway at the postsynaptic site and reveals a physiological function of the intramolecular SH3-GK domain interaction in SAP102

3D nanoscale analysis of bone healing around degrading Mg implants evaluated by X-ray scattering tensor tomography

The nanostructural adaptation of bone is crucial for its biocompatibility with orthopedic implants. The bone nanostructure also determines its mechanical properties and performance. However, the bone\u27s temporal and spatial nanoadaptation around degrading implants remains largely unknown. Here, we present insights into this important bone adaptation by applying scanning electron microscopy, elemental analysis, and small-angle X-ray scattering tensor tomography (SASTT). We extend the novel SASTT reconstruction method and provide a 3D scattering reciprocal space map per voxel of the sample\u27s volume. From this reconstruction, parameters such as the thickness of the bone mineral particles are quantified, which provide additional information on nanostructural adaptation of bone during healing. We selected a rat femoral bone and a degrading ZX10 magnesium implant as model system, and investigated it over the course of 18 months, using a sham as control. We observe that the bone\u27s nanostructural adaptation starts with an initially fast interfacial bone growth close to the implant, which spreads by a re-orientation of the nanostructure in the bone volume around the implant, and is consolidated in the later degradation stages. These observations reveal the complex bulk bone-implant interactions and enable future research on the related biomechanical bone responses. Statement of significance: Traumatic bone injuries are among the most frequent causes of surgical treatment, and often require the placement of an implant. The ideal implant supports and induces bone formation, while being mechanically and chemically adapted to the bone structure, ensuring a gradual load transfer. While magnesium implants fulfill these requirements, the nanostructural changes during bone healing and implant degradation remain not completely elucidated. Here, we unveil these processes in rat femoral bones with ZX10 magnesium implants and show different stages of bone healing in such a model system

Greenstone burial–exhumation cycles at the late Archean transition to plate tectonics

Converging lines of evidence suggest that, during the late Archean, Earth completed its transition from a stagnant-lid to a plate tectonics regime,although how and when this transition occurred is debated. The geological record indicates that some form of subduction, a key component of plate tectonics—has operated since the Mesoarchean, even though the tectonic style and timescales of burial and exhumation cycles within ancient convergent margins are poorly constrained.Here, we present a Neoarchean pressure–temperature–time (P–T–t) path from supracrustal rocks of the transpressional Yilgarn orogen (Western Australia), which documents how sea-floor-altered rocks underwent deep burial then exhumation during shortening that was unrelated to the episode of burial. Archean subduction, even if generally short-lived, was capable of producing eclogites along converging lithosphere boundaries, although exhumation processes in those environments were likely less efficient than today, such that return of high-pressure rocks to the surface was rare

Increased Error‐Related Brain Activity In Youth With Obsessive‐Compulsive Disorder And Unaffected Siblings

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/96293/1/da22035.pd

Competition between α-actinin and Ca2+-Calmodulin Controls Surface Retention of the L-type Ca2+ Channel CaV1.2

Regulation of neuronal excitability and cardiac excitation-contraction coupling requires proper localization of L-type Ca2+ channels. We show that the actin-binding protein α-actinin binds to the C-terminal surface targeting motif of α11.2, the central pore-forming CaV1.2 subunit, to foster its surface expression. Disruption of α-actinin function by dominant negative or shRNA constructs reduces CaV1.2 surface localization in HEK293 and neuronal cultures, and dendritic spine localization in neurons. We demonstrate that calmodulin displaces α-actinin from their shared binding site on α11.2 upon Ca2+ influx through L-type channels but not through NMDAR, thereby triggering loss of CaV1.2 from spines. Coexpression of a Ca2+-binding deficient calmodulin mutant does not affect basal CaV1.2 surface expression, but inhibits its internalization upon Ca2+ influx. We conclude that α-actinin stabilizes CaV1.2 at the plasma membrane, and that its displacement by Ca2+-calmodulin induces Ca2+-induced endocytosis of CaV1.2, thus providing an important negative feedback mechanism for Ca2+ influx

The role of leadership in salespeople’s price negotiation behavior

Salespeople assume a key role in defending firms’ price levels in price negotiations with customers. The degree to which salespeople defend prices should critically depend upon their leaders’ influence. However, the influence of leadership on salespeople’s price defense behavior is barely understood, conceptually or empirically. Therefore, building on social learning theory, the authors propose that salespeople might adopt their leaders’ price defense behavior given a transformational leadership style. Furthermore, drawing on the contingency leadership perspective, the authors argue that this adoption fundamentally depends on three variables deduced from the motivation–ability–opportunity (MAO) framework, that is, salespeople’s learning motivation, negotiation efficacy, and perceived customer lenience. Results of a multi-level model using data from 92 salespeople and 264 salesperson–customer interactions confirm these predictions. The first to explore contingencies of salespeople’s adoption of their transformational leaders’ price negotiation behaviors, this study extends marketing theory and provides actionable guidance to practitioners