310 research outputs found
Plasmid encoding matrix protein of vesicular stomatitis viruses as an antitumor agent inhibiting rat glioma growth in situ
Aim: Oncolytic effect of vesicular stomatitis virus (VSV) has been proved previously. Aim of the study is to investigate glioma inhibition effect of Matrix (M) protein of VSV in situ. Materials and Methods: A recombinant plasmid encoding VSV M protein (PM) was genetically engineered, and then transfected into cultured C6 gliomas cells in vitro. C6 transfected with Liposome-encapsulated PM (LEPM) was implanted intracranially for tumorigenicity study. In treatment experiment, rats were sequentially established intracranial gliomas with wild-typed C6 cells, and accepted LEPM injection intravenously. Possible mechanism of M protein was studied by using Hoechst staining, PI-stained flow cytometric analysis, TUNEL staining and CD31 staining. Results: M protein can induce generous gliomas lysis in vitro. None of the rats implanted with LEPM-treated cells developed any significant tumors, whereas all rats in control group developed tumors. In treatment experiment, smaller tumor volume and prolonged survival time was found in the LEPM-treated group. Histological studies revealed that possible mechanism were apoptosis and anti-angiogenesis. Conclusion: VSV-M protein can inhibit gliomas growth in vitro and in situ, which indicates such a potential novel biotherapeutic strategy for glioma treatment.Π¦Π΅Π»Ρ: ΠΈΠ·ΡΡΠΈΡΡ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ ΠΌΠ°ΡΡΠΈΠΊΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΠΈΠ½Π° (Π ΠΏΡΠΎΡΠ΅ΠΈΠ½Π°) Π²ΠΈΡΡΡΠ° Π²Π΅Π·ΠΈΠΊΡΠ»ΡΡΠ½ΠΎΠ³ΠΎ ΡΡΠΎΠΌΠ°ΡΠΈΡΠ° (ΠΠΠ‘) ΡΠ³Π½Π΅ΡΠ°ΡΡ ΡΠΎΡΡ Π³Π»ΠΈΠΎΠΌΡ
in situ. ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ: ΡΠΊΠΎΠ½ΡΡΡΡΠΈΡΠΎΠ²Π°Π½Π° ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½Π°Ρ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Π°, ΠΊΠΎΠ΄ΠΈΡΡΡΡΠ°Ρ Π ΠΏΡΠΎΡΠ΅ΠΈΠ½ ΠΠΠ‘, ΠΊΠΎΡΠΎΡΠ°Ρ Π·Π°ΡΠ΅ΠΌ Π±ΡΠ»Π°
ΡΡΠ°Π½ΡΡΠ΅ΡΠΈΡΠΎΠ²Π°Π½Π° Π² ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ Π³Π»ΠΈΠΎΠΌΡ Π‘6 in. ΠΠ»Π΅ΡΠΊΠΈ Π³Π»ΠΈΠΎΠΌΡ Π‘6, ΡΡΠ°Π½ΡΡΠ΅ΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΈΠ½ΠΊΠ°ΠΏΡΡΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌ
Π² Π»ΠΈΠΏΠΎΡΠΎΠΌΡ Π ΠΏΡΠΎΡΠ΅ΠΈΠ½ΠΎΠΌ (ΠΠΠΠ), ΠΈΠΌΠΏΠ»Π°Π½ΡΠΈΡΠΎΠ²Π°Π»ΠΈ ΠΈΠ½ΡΡΠ°ΠΊΡΠ°Π½ΠΈΠ°Π»ΡΠ½ΠΎ Π΄Π»Ρ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΡΡΠΌΠΎΡΠΎΠ³Π΅Π½Π½ΠΎΡΡΠΈ. Π ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ΅ ΠΊΡΡΡΠ°ΠΌ
Ρ ΡΡΠ°Π½ΡΠΏΠ»Π°Π½ΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΈΠ½ΡΡΠ°ΠΊΡΠ°Π½ΠΈΠ°Π»ΡΠ½ΠΎ Π³Π»ΠΈΠΎΠΌΠΎΠΉ Π‘6 (ΠΈΡΡ
ΠΎΠ΄Π½ΡΠΉ ΡΡΠ°ΠΌΠΌ) Π²Π½ΡΡΡΠΈΠ²Π΅Π½Π½ΠΎ Π²Π²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΠΠΠ. ΠΠΏΠΎΠΏΡΠΎΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅
Π ΠΏΡΠΎΡΠ΅ΠΈΠ½Π° Π½Π° ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ ΠΈΠ·ΡΡΠ°Π»ΠΈ Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠ΅Π½ΡΠ½ΠΎΠΉ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠΈΠΈ (ΠΎΠΊΡΠ°ΡΠΈΠ²Π°Π½ΠΈΠ΅ ΠΏΠΎ Π₯Π΅Ρ
ΡΡΡ),
ΠΏΡΠΎΡΠΎΡΠ½ΠΎΠΉ ΡΠΈΡΠΎΠΌΠ΅ΡΡΠΈΠΈ (ΠΎΠΊΡΠ°ΡΠΈΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠΎΠΏΠΈΠ΄ΠΈΡΠΌΠΎΠΌ ΠΉΠΎΠ΄ΠΈΠ΄ΠΎΠΌ), TUNEL Π²Π°ΡΠΊΡΠ»ΡΡΠΈΠ·Π°ΡΠΈΡ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ ΠΈ Π²Π°ΡΠΊΡΠ»ΡΡΠΈΠ·Π°ΡΠΈΡ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ ΠΈ
ΠΈΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈ Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ Π°Π½ΡΠΈ-CD31 ΠΌΠΎΠ½ΠΎΠΊΠ»ΠΎΠ½Π°Π»ΡΠ½ΡΡ
Π°Π½ΡΠΈΡΠ΅Π». 31 ΠΌΠΎΠ½ΠΎΠΊΠ»ΠΎΠ½Π°Π»ΡΠ½ΡΡ
Π°Π½ΡΠΈΡΠ΅Π». 31 ΠΌΠΎΠ½ΠΎΠΊΠ»ΠΎΠ½Π°Π»ΡΠ½ΡΡ
Π°Π½ΡΠΈΡΠ΅Π». Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: Π ΠΏΡΠΎΡΠ΅ΠΈΠ½ ΠΌΠΎΠΆΠ΅Ρ ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°ΡΡ
Π»ΠΈΠ·ΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ Π³Π»ΠΈΠΎΠΌΡ in. ΠΠΈ Ρ ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΆΠΈΠ²ΠΎΡΠ½ΠΎΠ³ΠΎ Ρ ΡΡΠ°Π½ΡΠΏΠ»Π°Π½ΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ Π³Π»ΠΈΠΎΠΌΡ, ΠΎΠ±ΡΠ°Π±ΠΎΡΠ°Π½Π½ΡΠΌΠΈ ΠΠΠΠ,
Π½Π΅ Π²ΠΎΠ·Π½ΠΈΠΊΠ°Π»ΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ°Π·ΠΌΠ΅ΡΠ°, ΡΠΎΠ³Π΄Π° ΠΊΠ°ΠΊ Ρ Π²ΡΠ΅Ρ
ΠΊΡΡΡ ΠΈΠ· ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΡ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΡΠ°Π·Π²ΠΈΠ²Π°Π»ΠΈΡΡ. Π Π³ΡΡΠΏΠΏΠ΅
ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
, ΠΊΠΎΡΠΎΡΡΠΌ Π²Π²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΠΠΠ, ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ Π±ΡΠ»ΠΈ ΠΌΠ΅Π½ΡΡΠ΅Π³ΠΎ ΠΎΠ±ΡΠ΅ΠΌΠ° ΠΈ ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΈ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΠΏΡΠΎΠ΄ΠΎΠ»ΠΆΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΠΆΠΈΠ·Π½ΠΈ
ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π ΠΏΡΠΎΡΠ΅ΠΈΠ½ ΠΏΡΠΎΡΠ²Π»ΡΠ΅Ρ Π°Π½ΡΠΈΠ°Π½Π³ΠΈΠΎΠ³Π΅Π½Π½ΡΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° ΠΈ ΠΎΠ±Π»Π°Π΄Π°Π΅Ρ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡΡ ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°ΡΡ Π°ΠΏΠΎΠΏΡΠΎΠ·.
ΠΡΠ²ΠΎΠ΄Ρ: Π ΠΏΡΠΎΡΠ΅ΠΈΠ½ ΠΠΠ‘ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΠ΅Ρ ΡΠΎΡΡ Π³Π»ΠΈΠΎΠΌΡ in ΠΈ in. ΠΠ° ΡΡΠΎΠΉ ΠΎΡΠ½ΠΎΠ²Π΅ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π° ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΠΎ Π½ΠΎΠ²Π°Ρ
Π±ΠΈΠΎΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΡΡΠ°ΡΠ΅Π³ΠΈΡ Π΄Π»Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π³Π»ΠΈΠΎΠΌΠ°ΠΌΠΈ
Surface Properties of SiCp/Al Composite by Powder-Mixed EDM
AbstractThis paper uses a kind of moderate volume fraction (40%) of SiC particle reinforced Al matrix composites (SiCp/Al) to research how the surface properties are affected in conventional EDM (EDM) and powder-mixed EDM (PMEDM). By means of environment scanning electron microscope (ESEM) and HIT friction and wear tester, surface micro-topography, elements and wear resistance were analyzed. Experiments and researches indicate that compared with EDM, the surface properties machined by using PMEDM are improved greatly. The PMEDM surface roughness decreases about 31.5%; corrosion resistance is better too; and wear resistance is twice of EDM. Powder-mixed EDM has promising applications in metal matrix composites machining field
Synthesis and crystal structure of <i>N</i>-(3-benzylamino-2- cyano-3-methylthioacrylyl)-<i>N'</i>-(substituted phenyl)ureas
Phenylurea groups were introduced into the frame of traditional cyanoacrylate and a series of N-(3-benzylamino-2-cyano-3-methylthioacrylyl)-N'-(substituted phenyl)ureas were synthesized. All compounds are new and their structures were confirmed by 1H NMR, 13C NMR and mass spectral analyses
The Conformal Willmore Functional: a Perturbative Approach
The conformal Willmore functional (which is conformal invariant in general
Riemannian manifold ) is studied with a perturbative method: the
Lyapunov-Schmidt reduction. Existence of critical points is shown in ambient
manifolds -where is a metric close
and asymptotic to the euclidean one. With the same technique a non existence
result is proved in general Riemannian manifolds of dimension three.Comment: 34 pages; Journal of Geometric Analysis, on line first 23 September
201
Serotonin accumulation in transgenic rice by over-expressing tryptophan decarboxlyase results in a dark brown phenotype and stunted growth
A mutant M47286 with a stunted growth, low fertility and dark-brown phenotype was identified from a T-DNA-tagged rice mutant library. This mutant contained a copy of the T-DNA tag inserted at the location where the expression of two putative tryptophan decarboxlyase genes, TDC-1 and TDC-3, were activated. Enzymatic assays of both recombinant proteins showed tryptophan decarboxlyase activities that converted tryptophan to tryptamine, which could be converted to serotonin by a constitutively expressed tryptamine 5' hydroxylase (T5H) in rice plants. Over-expression of TDC-1 and TDC-3 in transgenic rice recapitulated the stunted growth, dark-brown phenotype and resulted in a low fertility similar to M47286. The degree of stunted growth and dark-brown color was proportional to the expression levels of TDC-1 and TDC-3. The levels of tryptamine and serotonin accumulation in these transgenic rice lines were also directly correlated with the expression levels of TDC-1 and TDC-3. A mass spectrometry assay demonstrated that the dark-brown leaves and hulls in the TDC-overexpressing transgenic rice were caused by the accumulation of serotonin dimer and that the stunted growth and low fertility were also caused by the accumulation of serotonin and serotonin dimer, but not tryptamine. These results represent the first evidence that over-expression of TDC results in stunted growth, low fertility and the accumulation of serotonin, which when converted to serotonin dimer, leads to a dark brown plant color
The sintering, sintered microstructure and mechanical properties of Ti-Fe-Si Alloys
A systematic study has been conducted of the sintering, sintered microstructure and tensile properties of a range of lower cost Ti-Fe-Si alloys, including Ti-3Fe-(0-4)Si, Ti-(3-6)Fe-0.5Si, and Ti-(3-6)Fe-1Si (in wt pct throughout). Small additions of Si (β€1 pct) noticeably improve the as-sintered tensile properties of Ti-3Fe alloy, including the ductility, with fine titanium silicides (TiSi) being dispersed in both the Ξ± and Ξ² phases. Conversely, additions of >1 pct Si produce coarse and/or networked TiSi silicides along the grain boundaries leading to predominantly intergranular fracture and, hence, poor ductility, although the tensile strength continues to increase because of the reinforcement by TiSi. Increasing the Fe content in the Ti-xFe-0.5/ 1.0Si alloys above 3 pct markedly increases the average grain size and changes the morphology of the Ξ±-phase phase to much thinner and more acicular laths. Consequently, the ductility drops t
BCS and BEC p-wave pairing in Bose-Fermi gases
The pairing of fermionic atoms in a mixture of atomic fermion and boson gases
at zero temperature is investigated. The attractive interaction between
fermions, that can be induced by density fluctuations of the bosonic
background, can give rise to a superfluid phase in the Fermi component of the
mixture. The atoms of both species are assumed to be in only one internal
state, so that the pairing of fermions is effective only in odd-l channels. No
assumption about the value of the ratio between the Fermi velocity and the
sound velocity in the Bose gas is made in the derivation of the energy gap
equation. The gap equation is solved without any particular "ansatz" for the
pairing field or the effective interaction. The p-wave superfluidity is studied
in detail. By increasing the strength and/or decreasing the range of the
effective interaction a transition of the fermion pairing regime, from the
Bardeen-Cooper-Schrieffer state to a system of tightly bound couples can be
realized. These composite bosons behave as a weakly-interacting Bose-Einstein
condensate.Comment: 14 pages, 6 eps-figures. To be published in European Physical Journal
Measurements of the observed cross sections for exclusive light hadrons containing at , 3.650 and 3.6648 GeV
By analyzing the data sets of 17.3, 6.5 and 1.0 pb taken,
respectively, at , 3.650 and 3.6648 GeV with the BES-II
detector at the BEPC collider, we measure the observed cross sections for
, , ,
and at the three energy
points. Based on these cross sections we set the upper limits on the observed
cross sections and the branching fractions for decay into these
final states at 90% C.L..Comment: 7 pages, 2 figure
Partial wave analysis of J/\psi \to \gamma \phi \phi
Using events collected in the BESII detector, the
radiative decay is
studied. The invariant mass distribution exhibits a near-threshold
enhancement that peaks around 2.24 GeV/.
A partial wave analysis shows that the structure is dominated by a
state () with a mass of
GeV/ and a width of GeV/. The
product branching fraction is: .Comment: 11 pages, 4 figures. corrected proof for journa
Direct Measurements of Absolute Branching Fractions for D0 and D+ Inclusive Semimuonic Decays
By analyzing about 33 data sample collected at and around 3.773
GeV with the BES-II detector at the BEPC collider, we directly measure the
branching fractions for the neutral and charged inclusive semimuonic decays
to be and , and determine the ratio of the two branching
fractions to be
- β¦