Additive Fertigung frei geformter Betonbauteile durch selektives Binden mit calciumsilikatbasierten Zementen

Die additive Fertigung erlaubt ein bisher nicht gekanntes Maß an geometrischer Freiheit bei der Gestaltung von Bauteilen. In der Medizin- und Dentaltechnik wird die additive Fertigung beispielsweise eingesetzt, um an die individuelle Anatomie des Patienten angepasste Prothesen und Implantate herzustellen. Im Maschinenwesen ermöglicht sie den Bau von multifunktionalen und formoptimierten Bauteilen und damit eine Senkung des Materialeinsatzes bei gleichzeitiger Steigerung der Leistungsfähigkeit. In der Luft- und Raumfahrttechnik wird sie genutzt, um mittels Topologieoptimierung oder durch das Zusammenfassen von ganzen Baugruppen zu einem einzelnen Bauteil Gewicht zu sparen [1]. (Aus: Motivation]Additive manufacturing allows a previously unknown degree of geometric freedom in the design of components. In medical and dental technology for example, additive manufacturing is used to produce prostheses and implants adapted to the individual anatomy of the patient. In mechanical engineering, it enables the construction of multifunctional and shape-optimized components thus reducing the amount of material used while simultaneously increasing performance. In aerospace engineering, it is used to save weight by means of topology optimization or by combining entire assemblies into a single component [1]. [Off: Motivation

Industrieller 3D-Betondruck durch selektive Zementaktivierung - Verfahren, Material, Anwendungen

Die selektive Zementaktivierung (SCA) ist ein additives Fertigungsverfahren zur Herstellung von Bauteilen aus Beton. Die SCA gehört zu den Verfahren des selektiven Bindens, bei denen schüttfähiges Material (hier eine Trockenmischung aus Zement und Gesteinskörnung) in dünnen Schichten ausgebracht und durch Einbringen einer flüssigen Komponente (hier Wasser) selektiv gebunden wird. Verglichen mit anderen Verfahren der additiven Fertigung mit Beton sind bei der SCA sowohl die Auflösung als auch die geometrische Freiheit besonders hoch. ...Selective cement activation (SCA) is an additive manufacturing process for the fabrication of concrete elements. SCA belongs to the group of the selective binding processes in which bulk material (here a dry mixture of cement and aggregate) is spread in thin layers and selectively bound by applying a liquid component (here water). Compared to other additive manufacturing processes using concrete, both resolution and geometric freedom are particularly high with SCA. ..

Comprehensive Small RNA-Seq of Adeno-Associated Virus (AAV)-Infected Human Cells Detects Patterns of Novel, Non-Coding AAV RNAs in the Absence of Cellular miRNA Regulation

Most DNA viruses express small regulatory RNAs, which interfere with viral or cellular gene expression. For adeno-associated virus (AAV), a small ssDNA virus with a complex biphasic life cycle miRNAs or other small regulatory RNAs have not yet been described. This is the first comprehensive Illumina-based RNA-Seq analysis of small RNAs expressed by AAV alone or upon co-infection with helper adenovirus or HSV. Several hotspots of AAV-specific small RNAs were detected mostly close to or within the AAV-ITR and apparently transcribed from the newly identified anti-p5 promoter. An additional small RNA hotspot was located downstream of the p40 promoter, from where transcription of non- coding RNAs associated with the inhibition of adenovirus replication were recently described. Parallel detection of known Ad and HSV miRNAs indirectly validated the newly identified small AAV RNA species. The predominant small RNAs were analyzed on Northern blots and by human argonaute protein-mediated co-immunoprecipitation. None of the small AAV RNAs showed characteristics of bona fide miRNAs, but characteristics of alternative RNA processing indicative of differentially regulated AAV promoter-associated small RNAs. Furthermore, the AAV-induced regulation of cellular miRNA levels was analyzed at different time points post infection. In contrast to other virus groups AAV infection had virtually no effect on the expression of cellular miRNA, which underscores the long-established concept that wild-type AAV infection is apathogenic

Oxidized Phospholipids Predict the Presence and Progression of Carotid and Femoral Atherosclerosis and Symptomatic Cardiovascular Disease Five-Year Prospective Results From the Bruneck Study

ObjectivesThe purpose of this work was to determine the predictive value of oxidized phospholipids (OxPLs) present on apolipoprotein B-100 particles (apoB) in carotid and femoral atherosclerosis.BackgroundThe OxPLs are pro-inflammatory and pro-atherogenic and may be detected using the antibody E06 (OxPL/apoB).MethodsThe Bruneck study is a prospective population-based survey of 40- to 79-year-old men and women initiated in 1990. Plasma levels of OxPL/apoB and lipoprotein (a) [Lp(a)] were measured in 765 of 826 (92.6%) and 671 of 684 (98.1%) subjects alive in 1995 and 2000, respectively, and correlated with ultrasound measures of carotid and femoral atherosclerosis.ResultsThe distribution of the OxPL/apoB levels was skewed to lower levels and nearly identical to Lp(a) levels. The OxPL/apoB and Lp(a) levels were highly correlated (r = 0.87, p < 0.001), and displayed long-term stability and lacked correlations with most cardiovascular risk factors and lifestyle variables. The number of apolipoprotein (a) kringle IV-2 repeats was inversely related to Lp(a) mass (r = −0.48, p < 0.001) and OxPL/apoB levels (r = −0.46, p < 0.001). In multivariable analysis, OxPL/apoB levels were strongly and significantly associated with the presence, extent, and development (1995 to 2000) of carotid and femoral atherosclerosis and predicted the presence of symptomatic cardiovascular disease. Both OxPL/apoB and Lp(a) levels showed similar associations with atherosclerosis severity and progression, suggesting a common biological influence on atherogenesis.ConclusionsThis study suggests that pro-inflammatory oxidized phospholipids, present primarily on Lp(a), are significant predictors of the presence and extent of carotid and femoral atherosclerosis, development of new lesions, and increased risk of cardiovascular events. The OxPL biomarkers may provide valuable insights into diagnosing and monitoring cardiovascular disease

FuLeeca: A Lee-based Signature Scheme

In this work we introduce a new code-based signature scheme, called \textsf{FuLeeca}, based on the NP-hard problem of finding codewords of given Lee-weight. The scheme follows the Hash-and-Sign approach applied to quasi-cyclic codes. Similar approaches in the Hamming metric have suffered statistical attacks, which revealed the small support of the secret basis. Using the Lee metric, we are able to thwart such attacks. We use existing hardness results on the underlying problem and study adapted statistical attacks. We propose parameters for \textsf{FuLeeca}~and compare them to an extensive list of proposed post-quantum secure signature schemes including the ones already standardized by NIST. This comparison reveals that \textsf{FuLeeca}~is competitive. For example, for NIST category I, i.e., 160 bit of classical security, we obtain an average signature size of 1100 bytes and public key sizes of 1318 bytes. Comparing the total communication cost, i.e., the sum of the signature and public key size, we see that \textsf{FuLeeca} is only outperformed by Falcon while the other standardized schemes Dilithium and SPHINCS+ show larger communication costs than \textsf{FuLeeca}

Lipoprotein(a) and incident type-2 diabetes: results from the prospective Bruneck study and a meta-analysis of published literature.

AIMS: We aimed to (1) assess the association between lipoprotein(a) [Lp(a)] concentration and incident type-2 diabetes in the Bruneck study, a prospective population-based study, and (2) combine findings with evidence from published studies in a literature-based meta-analysis. METHODS: We used Cox proportional hazards models to calculate hazard ratios (HR) for incident type-2 diabetes over 20 years of follow-up in 815 participants of the Bruneck study according to their long-term average Lp(a) concentration. For the meta-analysis, we searched Medline, Embase and Web of Science for relevant prospective cohort studies published up to October 2016. RESULTS: In the Bruneck study, there was a 12% higher risk of type-2 diabetes for a one standard deviation lower concentration of log Lp(a) (HR = 1.12 [95% CI 0.95-1.32]; P = 0.171), after adjustment for age, sex, alcohol consumption, body mass index, smoking status, socioeconomic status, physical activity, systolic blood pressure, HDL cholesterol, log high-sensitivity C-reactive protein and waist-hip ratio. In a meta-analysis involving four prospective cohorts with a total of 74,575 participants and 4514 incident events, the risk of type-2 diabetes was higher in the lowest two quintiles of Lp(a) concentrations (weighted mean Lp(a) = 3.3 and 7.0 mg/dL, respectively) compared to the highest quintile (62.9 mg/dL), with the highest risk of type-2 diabetes seen in quintile 1 (HR = 1.28 [1.14-1.43]; P < 0.001). CONCLUSIONS: The current available evidence from prospective studies suggests that there is an inverse association between Lp(a) concentration and risk of type-2 diabetes, with a higher risk of type-2 diabetes at low Lp(a) concentrations (approximately <7 mg/dL)

Lipoprotein(a) and incident type-2 diabetes: results from the prospective Bruneck study and a meta-analysis of published literature

AIMS: We aimed to (1) assess the association between lipoprotein(a) [Lp(a)] concentration and incident type-2 diabetes in the Bruneck study, a prospective population-based study, and (2) combine findings with evidence from published studies in a literature-based meta-analysis. METHODS: We used Cox proportional hazards models to calculate hazard ratios (HR) for incident type-2 diabetes over 20 years of follow-up in 815 participants of the Bruneck study according to their long-term average Lp(a) concentration. For the meta-analysis, we searched Medline, Embase and Web of Science for relevant prospective cohort studies published up to October 2016. RESULTS: In the Bruneck study, there was a 12% higher risk of type-2 diabetes for a one standard deviation lower concentration of log Lp(a) (HR = 1.12 [95% CI 0.95-1.32]; P = 0.171), after adjustment for age, sex, alcohol consumption, body mass index, smoking status, socioeconomic status, physical activity, systolic blood pressure, HDL cholesterol, log high-sensitivity C-reactive protein and waist-hip ratio. In a meta-analysis involving four prospective cohorts with a total of 74,575 participants and 4514 incident events, the risk of type-2 diabetes was higher in the lowest two quintiles of Lp(a) concentrations (weighted mean Lp(a) = 3.3 and 7.0 mg/dL, respectively) compared to the highest quintile (62.9 mg/dL), with the highest risk of type-2 diabetes seen in quintile 1 (HR = 1.28 [1.14-1.43]; P < 0.001). CONCLUSIONS: The current available evidence from prospective studies suggests that there is an inverse association between Lp(a) concentration and risk of type-2 diabetes, with a higher risk of type-2 diabetes at low Lp(a) concentrations (approximately <7 mg/dL).The Bruneck Study was supported by the ‘Pustertaler Verein zur Prävention von Herz- und Hirngefaesserkrankungen, Gesundheitsbezirk Bruneck’ and the ‘Assessorat für Gesundheit’, Province of Bolzano, Italy, and an excellence initiative (Competence Centers for Excellent Technologies-COMET) of the Austrian Research Promotion Agency FFG: “Research Center of Excellence in Vascular Ageing—Tyrol, VASCage” (K-Project Number 843536). Peter Willeit was supported by an Erwin-Schrödinger-Fellowship sponsored by the Austrian Science Fund (J-3679-B13)