Fluorescence studies of the binding of amphiphilic amines with phospholipids.

The binding characteristics of several amine drugs with dispersed phospholipids (phosphatidylcholine, phosphatidylserine, and phosphatidylglycerol) have been studied using the fluorometric method and 1-anilino-8-naphthalene sulfonate and 1,6 diphenyl-1,3,5-hexatriene as fluorescence probes. The results show that amphiphilic amines, such as chlorphentermine, interact with phospholipids via both ionic and hydrophobic forces. The ionic interaction, which occurs between the protonated amine group of the drug and the phosphate oxygen of the lipid, changes the amphiphilic characteristics of the lipid by reducing the number of negative charges on the lipid vesicles, and inhibits the Ca2+-dependent lipid hydrolysis by blocking the Ca2+ binding sites on the lipid vesicles. The hydrophobic interaction, which involves the nonpolar moieties of the drug and the lipid, is of primary importance to the overall drug-lipid binding stability. Drugs without a strong hydrophobic moiety, such as dopamine, do not interact with phospholipids

Sivers function in light-cone quark model and azimuthal spin asymmetries in pion electroproduction

We perform a calculation of Sivers function in a light-cone SU(6) quark-diquark model with both scalar diquark and vector diquark spectators. We derive the transverse momentum dependent light-cone wave function of the proton by taking into account the Melosh-Wigner rotation. By adopting one-gluon exchange, we obtain a non-vanishing Sivers function of $down$ quark from interference of proton spin amplitudes. We analyze the $\frac{|P_{h\perp}|}{M}$ weighted Sivers asymmetries in $\pi^+$, $\pi^-$ and $\pi^0$ electroproduction off transverse polarized proton target, averaged and not averaged by the kinematics of HERMES experiment.Comment: 17 LaTex pages, 2 figures. Final version for journal publicatio

Providing Integrated Life Cycle Support in Process-Aware Information Systems

The need for more flexibility of process-aware information systems (PAISs) has been discussed for several years and different approaches for adaptive process management have emerged. However, only few of them provide support for both changes of individual process instances and the propagation of process type changes to a collection of related process instances. Furthermore, knowledge about process changes has not yet been exploited by any of these systems. This paper presents the ProCycle approach which overcomes this practical limitation by capturing the whole process life cycle and all kinds of changes in an integrated way. Users are not only allowed to deviate from the predefined process in exceptional situations, but are also assisted in retrieving and reusing knowledge about previously performed changes in this context. If similar instance deviations occur frequently, process engineers will be supported in deriving improved process models from them. This, in turn, allows engineers to evolve the PAIS (including the knowledge about the changes) over time. Feasability of the ProCycle approach is demonstrated by a proof-of-concept prototype which combines adaptive process management technology with concepts and methods provided by case-based reasoning (CBR) technology

Pion-photon and photon-pion transition form factors in light-cone formalism

We derive the minimal Fock-state expansions of the pion and the photon wave functions in light-cone formalism, then we calculate the pion-photon and the photon-pion transition form factors of $\gamma ^{\ast}\pi ^{0}\to \gamma$ and $\gamma ^{\ast}\gamma \to \pi ^{0}$ processes by employing these quark-antiquark wave functions of the pion and the photon. We find that our calculation for the $\gamma ^{\ast}\gamma \to \pi ^{0}$ transition form factor agrees with the experimental data at low and moderately high energy scale. Moreover, the physical differences and inherent connections between the transition form factors of $\gamma ^{\ast}\pi ^{0}\to \gamma$ and $\gamma ^{\ast}\gamma \to \pi ^{0}$ have been illustrated, which indicate that these two physical processes are intrinsically related. In addition, we also discuss the $\pi ^{0}\to \gamma \gamma$ form factor and the decay width $\mathit{\Gamma}(\pi \to \gamma \gamma)$ at $Q^{2}=0$.Comment: 20 pages, 2 figure

Combination antiretroviral therapy improves cognitive performance and functional connectivity in treatment-naïve HIV-infected individuals.

Our study aimed to investigate the short-term effect of combination antiretroviral therapy (cART) on cognitive performance and functional and structural connectivity and their relationship to plasma levels of antiretroviral (ARV) drugs. Seventeen ARV treatment-naïve HIV-infected individuals (baseline mean CD4 cell count, 479 ± 48 cells/mm3) were age matched with 17 HIV-uninfected individuals. All subjects underwent a detailed neurocognitive and functional assessment and magnetic resonance imaging. HIV-infected subjects were scanned before starting cART and 12 weeks after initiation of treatment. Uninfected subjects were assessed once at baseline. Functional connectivity (FC) was assessed within the default mode network while structural connectivity was assessed by voxel-wise analysis using tract-based spatial statistics (TBSS) and probabilistic tractography within the DMN. Tenofovir and emtricitabine blood concentration were measured at week 12 of cART. Prior to cART, HIV-infected individuals had significantly lower cognitive performance than control subjects as measured by the total Z-score from the neuropsychological tests assessing six cognitive domains (p = 0.020). After 12 weeks of cART treatment, there remained only a weak cognitive difference between HIV-infected and HIV-uninfected subjects (p = 0.057). Mean FC was lower in HIV-infected individuals compared with those uninfected (p = 0.008), but FC differences became non-significant after treatment (p = 0.197). There were no differences in DTI metrics between HIV-infected and HIV-uninfected individuals using the TBSS approach and limited evidence of decreased structural connectivity within the DMN in HIV-infected individuals. Tenofovir and emtricitabine plasma concentrations did not correlate with either cognitive performance or imaging metrics.ConclusionsTwelve weeks of cART improves cognitive performance and functional connectivity in ARV treatment-naïve HIV-infected individuals with relatively preserved immune function. Longer periods of observation are necessary to assess whether this effect is maintained

Intercomparisons of airborne measurements of aerosol ionic chemical composition during TRACE-P and ACE-Asia

As part of the two field studies, Transport and Chemical Evolution over the Pacific (TRACE-P) and the Asian Aerosol Characterization Experiment (ACE-Asia), the inorganic chemical composition of tropospheric aerosols was measured over the western Pacific from three separate aircraft using various methods. Comparisons are made between the rapid online techniques of the particle into liquid sampler (PILS) for measurement of a suite of fine particle a mist chamber/ion chromatograph (MC/IC) measurement of fine sulfate, and the longer time-integrated filter and micro-orifice impactor (MOI) measurements. Comparisons between identical PILS on two separate aircraft flying in formation showed that they were highly correlated (e.g., sulfate r2 of 0.95), but were systematically different by 10 ± 5% (linear regression slope and 95% confidence bounds), and had generally higher concentrations on the aircraft with a low-turbulence inlet and shorter inlet-to-instrument transmission tubing. Comparisons of PILS and mist chamber measurements of fine sulfate on two different aircraft during formation flying had an r 2 of 0.78 and a relative difference of 39% ± 5%. MOI ionic data integrated to the PILS upper measurement size of 1.3 mm sampling from separate inlets on the same aircraft showed that for sulfate, PILS and MOI were within 14% ± 6% and correlated with an r 2 of 0.87. Most ionic compounds were within ±30%, which is in the range of differences reported between PILS and integrated samplers from ground-based comparisons. In many cases, direct intercomparison between the various instruments is difficult due to differences in upper-size detection limits. However, for this study, the results suggest that the fine particle mass composition measured from aircraft agree to within 30–40%