Human Trafficking and Foreign Policy: An Introduction

[Excerpt] Human trafficking (also known as trafficking in persons) refers to the subjection of men, women, or children to exploitative conditions that may be tantamount to modern-day slavery. From a foreign policy perspective, human trafficking can be viewed as a human rights problem, a manifestation of transnational organized crime, and a violation of core international labor standards. Human trafficking also raises economic development, international migration, and global governance and security issues, and disproportionately victimizes vulnerable populations. The Trafficking Victims Protection Act of 2000 (TVPA, Division A of P.L. 106-386; 22 U.S.C. 7101 et seq.) defined “severe forms of trafficking in persons” to include sex trafficking induced by force, fraud, or coercion, child sex trafficking (under 18 years of age), and forced labor trafficking. The latter involves the recruitment, harboring, transportation, provision, or obtaining of a person—induced by force, fraud, or coercion—for the purpose of subjecting that person, including a child, to involuntary servitude, peonage, debt bondage, or slavery

A 45-year-old Female with an Atypical Presentation of Pharyngitis

Introduction: Emergency physicians are trained to treat a variety of ailments in the emergency department (ED), some of which are emergent, while others are not. A common complaint seen in the ED is a sore throat. While most sore throats are easily diagnosed and treated, less common causes are often not considered in the differential diagnoses. Therefore, the purpose of this case study was to present an atypical case of sore throat and discuss differential diagnoses.Case Presentation: The patient was a 45-year-old female who presented to the ED with a three-day history of sore throat that was exacerbated by eating and drinking. The patient was not on any prescription medications, but tried over-the-counter medications for the sore throat without any improvement in symptoms. Review of systems was positive for sore throat, fevers, and chills. Physical examination of her oropharynx revealed mildly dry mucous membranes with confluent plaques and white patchy ulcerative appearance involving the tongue, tonsils, hard palate, and soft palate. Rapid streptococcal antigen, mononucleosis spot test, and KOH test were performed and found to be negative.Discussion: After initial testing was negative, a follow-up complete blood count with differential and complete metabolic profile were ordered. The patient was found to have decreased lymphocytes and platelets. Based upon those results, a diagnosis was made in the ED, the patient was started on medication, and further laboratory workup was ordered to confirm the diagnosis. ED providers should consider non-infectious as well as infectious causes for a sore throat, as this might lead to a diagnosis of an underlying condition

Stellar rotation, binarity, and lithium in the open cluster IC4756

An important aspect in the evolutionary scenario of cool stars is their rotation and the rotationally induced magnetic activity and interior mixing. Stars in open clusters are particularly useful tracers for these aspects because of their known ages. We aim to characterize the open cluster IC4756 and measure stellar rotation periods and surface differential rotation for a sample of its member stars. Thirty-seven cluster stars were observed continuously with the CoRoT satellite for 78 days in 2010. Follow-up high-resolution spectroscopy of the CoRoT targets and deep Str\"omgren $uvby\beta$ and H$\alpha$ photometry of the entire cluster were obtained with our robotic STELLA facility and its echelle spectrograph and wide-field imager, respectively. We determined high-precision photometric periods for 27 of the 37 CoRoT targets and found values between 0.155 and 11.4 days. Twenty of these are rotation periods. Twelve targets are spectroscopic binaries of which 11 were previously unknown; orbits are given for six of them. Six targets were found that show evidence of differential rotation with $\Delta\Omega/\Omega$ in the range 0.04-0.15. Five stars are non-radially pulsating stars with fundamental periods of below 1d, two stars are semi-contact binaries, and one target is a micro-flaring star that also shows rotational modulation. Nine stars in total were not considered members because of much redder color(s) and deviant radial velocities with respect to the cluster mean. H$\alpha$ photometry indicates that the cluster ensemble does not contain magnetically over-active stars. The cluster average metallicity is -0.08$\pm$0.06 (rms) and its logarithmic lithium abundance for 12 G-dwarf stars is 2.39$\pm$0.17 (rms). [...]Comment: A&A, in pres

Sr-isotope analysis of speleothems by LA-MC-ICP-MS: High temporal resolution and fast data acquisition

Speleothems are well established climate archives. A wide array of geochemical proxies, including stable isotopes and trace elements are present within speleothems to reconstruct past climate variability. However, each proxy is influenced by multiple factors, often hampering robust interpretation. Sr isotope ratios (87Sr/86Sr) can provide useful information about water residence time and water mixing in the host rock, as they are not fractionated during calcite precipitation. Laser ablation multi-collector-inductively coupled plasma mass spectrometry (LA-MC-ICP-MS) has rarely been used for determination of Sr isotope signatures in speleothems, as speleothems often do not possess appropriately high concentrations of Sr to facilitate this analysis. Yet the advantages of this approach include rapid data acquisition, higher spatial resolution, larger sample throughput and the absence of chemical treatment prior to analysis. We present LA-MC-ICP-MS Sr isotope data from two speleothems from Morocco (Grotte de Piste) and India (Mawmluh Cave), and we compare linescan and spot analysis ablation techniques along speleothem growth axes. The analytical uncertainty of our LA-MC-ICP-MS Sr data is comparable to studies conducted on other carbonate materials. The results of both ablation techniques are reproducible within analytical error, implying that this technique yields robust results when applied to speleothems. In addition, several comparative measurements of different carbonate reference materials (i.e. MACS-3, JCt-1, JCp-1), including tests with standard bracketing and comparison of the 87Sr/86Sr ratios with a nanosecond laser ablation system and a state-of-the-art femtosecond laser ablation system, highlight the robustness of the method

Seminal fluid-mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano

Weber M, Giannakara A, Ramm SA. Seminal fluid-mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano. Ecology and evolution. 2019;9(24):13889-13901.As a class, seminal fluid proteins are expected to exert strong effects on mating partners due to the selection pressures of sperm competition and sexual conflict. But because of the complexity of this secretion, linking specific proteins to downstream effects on own fitness-via manipulating the reproductive behavior, physiology, and ultimately the sperm utilization of mating partners-is not straightforward. Here, we adopted a systematic gene knockdown approach to screen for seminal fluid-mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano. We focused on 18 transcripts in M. lignano seminal fluid, testing how their RNA interference-induced knockdown impacted on three aspects of donor (male) reproductive success: (a) fertility (offspring production of the partner); (b) defensive sperm competitive ability, P-1; and (c) offensive sperm competitive ability, P-2. In general, the knockdown of most individual transcripts appeared to have only a minor impact on male reproductive success, though we found evidence that the knockdown of up to five different transcripts impacted on fertility; the knockdown of two other transcripts resulted in reduced P-2; and knockdown of a further transcript actually increased P-2. We thus identify a number of candidate seminal fluid transcripts that appear to modulate offspring production and sperm competitiveness in M. lignano. That only a minority of transcripts exhibit such a pattern likely reflects both the difficulty of accurately estimating sperm competitiveness and the functional redundancy of seminal fluid

Effects of two seminal fluid transcripts on post‐mating behavior in the simultaneously hermaphroditic flatworm Macrostomum lignano

Weber M, Patlar B, Ramm SA. Effects of two seminal fluid transcripts on post‐mating behavior in the simultaneously hermaphroditic flatworm Macrostomum lignano. Journal of Evolutionary Biology. 2020;22(5): jeb.13606.The seminal fluid proteins (SFPs) transferred to mating partners along with sperm often play crucial roles in mediating post-mating sexual selection. One way in which sperm donors can maximize their own reproductive success is by modifying the partners' (sperm recipient's) post-copulatory behavior to prevent or delay re-mating, thereby decreasing the likelihood or intensity of sperm competition. Here, we adopted a quantitative genetic approach combining gene expression and behavioral data to identify candidates that could mediate such a response in the simultaneously hermaphroditic flatworm Macrostomum lignano. We identified two putative SFPs - Mlig-pro46 and Mlig-pro63 - linked to both mating frequency and 'suck' frequency, a distinctive behavior, in which, upon ejaculate receipt, the worm places its pharynx over its female genital opening and apparently attempts to remove the received ejaculate. We, therefore, performed a manipulative experiment using RNA interference-induced knockdown to ask how the loss of Mlig-pro46 and Mlig-pro63 expression, singly and in combination, affects mating frequency, partner suck propensity and sperm competitive ability. None of the knock-down treatments impacted strongly on the mating frequency or sperm competitive ability, but knock-down of Mlig-pro63 resulted in a significantly decreased 'suck' propensity of mating partners. This suggests that Mlig-pro63 may normally act as a cue in the ejaculate to trigger recipient suck behavior, and - given that other proteins in the ejaculate have the opposite effect - could be one component of an on-going arms race between donors and recipients over the control of ejaculate fate. However, the adaptive significance of Mlig-pro46 and Mlig-pro63 from a donor perspective remains enigmatic. This article is protected by copyright. All rights reserved

CGM2, a Member of the Carcinoembryonic Antigen Gene Family is Down- Regulated in Colorectal Carcinomas

We have determined the precise chromosomal location, the exon structure, and the expression pattern of CGM2, a member of the carcinoembryonic antigen (CEA) gene family. CGM2 cDNA was amplified by reverse transcription-polymerase chain reaction (RT/PCR) from the colon adenocarcinoma cell line, LS174T. A defective exon is missing from this cDNA clone, leading to a novel domain organization for the human CEA family with two immunoglobulin-like domains. The derived C-terminal domain predicts that the CGM2 protein is membrane-bound through a glycosyl phosphatidylinositol anchor. RT/PCR analyses identified CGM2 transcripts in mucinous ovarian and colonic adenocarcinomas as well as in adjacent colonic tissue, but not in other tumors including leukocytes from six chronic myeloid leukemia patients. Thus, unlike several other family members, CGM2 is not expressed in granulocytes but reveals a more CEA-like expression pattern. Northern blot analyses identified a 2.5-kilobase CGM2 mRNA that is strongly down-regulated in colonic adenocarcinomas compared with adjacent colonic mucosa, suggesting a possible tumor suppressor function. In addition, a 3.2- kilobase transcript was observed in a number of colon tumors that is not detectable in normal colonic tissue. This mRNA species could represent a tumor-specific CGM2 splice variant