In-stream habitat predicts salamander occupancy and abundance better than landscape-scale factors within exurban watersheds in a global diversity hotspot

The southern Appalachian Mountains have experienced rapid human population growth rates since the 1980s. Land used practices are shifting from rural to residential. The majority of development has been low density, and is often near biologically diverse areas such as National Forests and National Parks. The long-term effects of urbanization in the southeastern Appalachian Mountains are not clearly understood and even less is known with respect to stream salamander response to urbanization. In order to determine the temporal influence of exurban housing on southern Appalachian streams we sampled 27 first- and second-order streams in watersheds containing exurban developments ranging in age from 4 to 44 years, along with eight forested streams, over the course of two summers. We sought to determine if the relative age of an exurban development related to occupancy and abundance of southern Appalachian stream salamanders. Age of exurban development and other watershed scale variables were not top predictors of salamander assemblages, while local site variables such as salinity and undercut banks predicted the abundance of several species of salamander. Our results suggest local habitat improvements can be used to better conserve salamanders and stream ecosystems in an increasingly urbanized region

The influence of exurban landscapes and local site characteristics on riparian vegetation

The southern Appalachian Mountains have experienced large population growth and a change in land use in the past 30 years. The majority of development has been low density, suburban land, known as exurban development. The long-term effects of exurbanization on riparian vegetative communities in the southeastern Appalachian Mountains are not well known. We sought to determine if vegetative community composition and structure change as a function of watershed–level variables such as time since neighborhood development or percent impervious surface within the watershed. We also assessed local–scale measures of disturbance such as canopy cover and basal area. Over two years we sampled a total of 27 streams in exurban watersheds ranging in age from four to forty-four years, along with eight forested streams. Watershed–scale variables such as neighborhood age and impervious surface cover did not influence the aspects of riparian vegetation community we measured. Canopy cover, a measure of local habitat disturbance, offered better predictions of vegetation community metrics. Exurban neighborhoods and their landowners may have the potential to manage for riparian vegetation through the use of maintained stream buffer zones along the entire length of the stream

The Future of Natural History Transcription: Navigating AI advancements with VoucherVision and the Specimen Label Transcription Project (SLTP)

Natural history collections are critical reservoirs of biodiversity information but collections staff are constantly grappling with substantial backlogs and limited resources. The task of transcribing specimen label text into searchable databases requires a significant amount of time, manual labor, and funding. To address this challenge, we introduce VoucherVision, a tool harnessing the capabilities of several Large Language Models (LLMs; Naveed et al. 2023) to augment specimen label transcription. The VoucherVision tool automates laborious components of the transcription process, leveraging an Optical Character Recognition (OCR) system and LLMs to convert unstructured label text into appropriate data formats compatible with database ingestion. VoucherVision uses a combination of structured output parsers and recursive re-prompting strategies to ensure consistency and quality of the LLM-formatted text, significantly reducing errors.Integration of VoucherVision with the University of Michigan Herbarium's transcription workflow resulted in a significant reduction in per-image transcription time, suggesting significant potential advantages for collections workflows. VoucherVision offers promising strides towards efficient digitization, with curatorial staff playing critical roles in data quality assurance and process oversight. Emphasizing the importance of knowledge sharing, the University of Michigan Herbarium is backing the Specimen Label Transcription Project (SLTP), which will provide open access to benchmarking datasets, fine-tuned models, and validation tools to rank the performance of different methodologies, LLMs, and prompting strategies. In the rapidly evolving landscape of Artificial Intelligence (AI) development, we recognize the profound potential of diverse contributions and innovative methodologies to redefine and advance the transformation of curatorial practices, catalyzing an era of accelerated digitization in natural history collections.An early, public version of VoucherVision is available to try here: https://vouchervision.azurewebsites.net

Basic Features of a Cell Electroporation Model: Illustrative Behavior for Two Very Different Pulses

Science increasingly involves complex modeling. Here we describe a model for cell electroporation in which membrane properties are dynamically modified by poration. Spatial scales range from cell membrane thickness (5 nm) to a typical mammalian cell radius (10 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}\upmu\end{document}m), and can be used with idealized and experimental pulse waveforms. The model consists of traditional passive components and additional active components representing nonequilibrium processes. Model responses include measurable quantities: transmembrane voltage, membrane electrical conductance, and solute transport rates and amounts for the representative “long” and “short” pulses. The long pulse—1.5 kV/cm, 100 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}\upmu\end{document}s—evolves two pore subpopulations with a valley at \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\sim}$$\end{document}5 nm, which separates the subpopulations that have peaks at \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\sim}$$\end{document}1.5 and \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\sim}$$\end{document}12 nm radius. Such pulses are widely used in biological research, biotechnology, and medicine, including cancer therapy by drug delivery and nonthermal physical tumor ablation by causing necrosis. The short pulse—40 kV/cm, 10 ns—creates 80-fold more pores, all small (\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$<$$\end{document}3 nm; \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\sim$$\end{document}1 nm peak). These nanosecond pulses ablate tumors by apoptosis. We demonstrate the model’s responses by illustrative electrical and poration behavior, and transport of calcein and propidium. We then identify extensions for expanding modeling capability. Structure-function results from MD can allow extrapolations that bring response specificity to cell membranes based on their lipid composition. After a pulse, changes in pore energy landscape can be included over seconds to minutes, by mechanisms such as cell swelling and pulse-induced chemical reactions that slowly alter pore behavior. Electronic supplementary material The online version of this article (doi:10.1007/s00232-014-9699-z) contains supplementary material, which is available to authorized users

Assessment of a Novel Pediatric Resident Simulation Curriculum

Aim: To assess the efficacy of a newly implemented resident simulation curriculum at a medium sized pediatric residency program. Background: Many pediatric residency programs incorporate high-fidelity simulation into their curriculum, but there is limited data discussing the utility/educational impacts of a longitudinal/standardized/multimodal simulation curriculum. Several studies of simulation-based training have employed “self-efficacy” as a barometer for trainee education and performance 1,2,. The level of a person’s self-efficacy can influence their behavior and may be a pivotal factor in performance. We have implemented a newly devised standardized, multimodal resident simulation curriculum and used resident self-efficacy to assess its effectiveness. Methods: Participants were UMass Pediatric and Med/Peds residents. Implementation of our curriculum occurred at the start of the 2016-2017 academic year. Surveys were administered to all residents prior to curriculum implementation and at 6 months post-implementation. They assessed resident self-efficacy with regards to specific technical/procedural skills (i.e. running a code, performing intubation, etc.) and resident confidence in their ability to identify/manage specific pediatric disease presentations (i.e. respiratory failure, tachyarrhythmia, etc.). Data was pooled and averaged for each resident class separately. We predetermined a 10% change in self-efficacy to be a clinically significant difference. Results: 36 of 40 residents completed the initial survey and 31 completed the 6-month follow-up. PGY1 residents reported improved self-efficacy for 4 PALS-related skills and 8 pediatric case presentations. Similarly, PGY2 residents reported improved self-efficacy for 3 PALS-related skills and 6 pediatric case presentations. Conversely, PGY3/4 residents reported no significant change in self-efficacy for any survey question. Conclusions: These results suggest that our newly implemented longitudinal, standardized, multidisciplinary, multi-modal simulation curriculum has significantly improved resident self-efficacy related to core Pediatric Advanced Life-Support (PALS) skills/topics, with the greatest impact affecting our PGY1 class. Further study and curriculum development will attempt to address this issue

Perceptions of Dry Eye Disease Management in Current Clinical Practice

To assess the perceptions of eye care providers regarding the clinical management of dry eye

Dynamic evolution in the key honey bee pathogen deformed wing virus: Novel insights into virulence and competition using reverse genetics

The impacts of invertebrate RNA virus population dynamics on virulence and infection out- comes are poorly understood. Deformed wing virus (DWV), the main viral pathogen of honey bees, negatively impacts bee health, which can lead to colony death. Despite previ- ous reports on the reduction of DWV diversity following the arrival of the parasitic mite Var- roa destructor, the key DWV vector, we found high genetic diversity of DWV in infested United States honey bee colonies. Phylogenetic analysis showed that divergent US DWV genotypes are of monophyletic origin and were likely generated as a result of diversification after a genetic bottleneck. To investigate the population dynamics of this divergent DWV, we designed a series of novel infectious cDNA clones corresponding to coexisting DWV genotypes, thereby devising a reverse-genetics system for an invertebrate RNA virus qua- sispecies. Equal replication rates were observed for all clone-derived DWV variants in single infections. Surprisingly, individual clones replicated to the same high levels as their mixtures and even the parental highly diverse natural DWV population, suggesting that complemen- tation between genotypes was not required to replicate to high levels. Mixed clone–derived infections showed a lack of strong competitive exclusion, suggesting that the DWV geno- types were adapted to coexist. Mutational and recombination events were observed across clone progeny, providing new insights into the forces that drive and constrain virus diversifi- cation. Accordingly, our results suggest that Varroa influences DWV dynamics by causing an initial selective sweep, which is followed by virus diversification fueled by negative fre- quency-dependent selection for new genotypes. We suggest that this selection might reflect the ability of rare lineages to evade host defenses, specifically antiviral RNA interference (RNAi). In support of this hypothesis, we show that RNAi induced against one DWV strain is less effective against an alternate strain from the same population

Serologic Evidence of Various Arboviruses Detected in White Tailed Deer (\u3ci\u3eOdocoileus virginianus\u3c/i\u3e) in the United States

White-tailed deer (Odocoileus virginianus) are an abundant mammal with a wide geographic distribution in the United States, which make them good sentinels for monitoring arboviral activity across the country. Exposure to various arboviruses has been detected in white-tailed deer, typically in conjunction with another diagnostic finding. To better assess the exposure of white-tailed deer to seven arboviruses, wetested 1,508 sera collected from 2010 to 2016 for antibodies to eastern equine encephalitis (2.5%), Powassan (4.2%), St. Louis encephalitis, (3.7%), West Nile (6.0%), Maguari (19.4%), La Crosse (30.3%), and bluetongue (7.8%) viruses. At least one arbovirus was detected in 51.3%, and exposure to more than one arbovirus was identified in 17.6% of the white-tailed deer sampled