OGO-E cosmic radiation - Nuclear abundance experiment

OGO-E cosmic radiation detector

A mechanistic-empirical based overlay design method for reflective cracking

This paper describes a new and innovative mechanistically based pavement overlay design method that considers the most predominant type of overlay distress observed in the field: Reflective cracking above old cracks in the underlying pavement surface. Both dense-graded hot mix asphalt and gap-graded asphalt rubber (wet process) mixes were studied, in the laboratory and in the field, to derive the necessary mechanistic relationships and statistically based equations. The models proposed are based on a finite element model that closely approximates actual field phenomena. Many field test sections, in Arizona, California and Portugal, were studied during the course of the research. Other HMA mixes used for overlays may also be calibrated and used through the proposed method. However, the relevant mix properties of any additional materials or environmental zones must first be determined. The two mix types studied are mainly used in the desert southwest region of Arizona and California. The overlay design program is available from the Rubber Pavements Association or Arizona Department of Transportation in the form of an Excel spreadsheet with an easy-to-use visual basic computer program (macro)

Lysine-Cysteine-Lysine (KCK) tag changes ParB action in vitro but not in vivo

Due to the enhanced labeling capability of maleimide-based fluorescent probes in in vitro experiments, lysine-cysteine-lysine (KCK) tags are frequently added to proteins for visualization. Here we show that, although no noticeable changes were detected from in vivo fluorescence imaging and chromatin immunoprecipitation (ChIP) assays, the KCK-tag substantially altered DNA compaction rates by Bacillus subtilis ParB protein in in vitro single-molecule DNA flow-stretching experiments. Furthermore, our measurements and statistical analyses demonstrate that the KCK-tags also altered the ParB protein’s response to nucleotide (cytidine triphosphate CTP or its nonhydrolyzable analog CTPγS) binding and the presence of the specific DNA binding sequence (parS). Remarkably, the appended KCK-tags are capable of even reversing the trends of DNA compaction rates upon different experimental conditions. DNA flow-stretching experiments for both fluorescently-labeled ParB proteins and ParB proteins with an N-terminal glutamic acid-cysteine-glutamic acid (ECE) tag support the notion that electrostatic interactions between charges on the tags and the DNA backbone are an underlying cause of the protein’s property changes. While it is typically assumed that the short KCK-tag minimally perturbs protein function, our results demonstrate that this assumption must be carefully tested when using tags for protein labeling

The association between cardiorespiratory fitness, liver fat and insulin resistance in adults with or without type 2 diabetes : a cross-sectional analysis

Background: Exercise-induced improvements in cardiorespiratory fitness (CRF) often coincide with improvements in insulin sensitivity and reductions in liver fat content. However, there are limited data concerning the relationship between CRF and liver fat content in adults with varying degrees of metabolic dysfunction. Methods: The aim of this study was to examine the association between CRF, liver fat content, and insulin resistance in inactive adults with obesity and with or without type 2 diabetes (T2D), via cross-sectional analysis. CRF was determined via a graded exercise test. Liver fat content was assessed via proton magnetic resonance spectroscopy and insulin resistance was assessed via homeostatic model of insulin resistance (HOMA-IR). A partial correlation analysis, controlling for age and gender, was performed to determine the association between CRF, demographic, cardiometabolic, and anthropometric variables. Independent t tests were performed to compare cardiometabolic outcomes between participants with T2D and participants without T2D. Results: Seventy-two adults (46% male) with a mean age of 49.28 ± 10.8 years, BMI of 34.69 ± 4.87 kg/m2 , liver fat content of 8.37 ± 6.90%, HOMA-IR of 3.07 ± 2.33 and CRF of 21.52 ± 3.77 mL/kg/min participated in this study. CRF was inversely associated with liver fat content (r = − 0.28, p = 0.019) and HOMA-IR (r = − 0.40, p < 0.001). Participants with T2D had significantly higher liver fat content (+ 3.66%, p = 0.024) and HOMA-IR (+ 2.44, p < 0.001) than participants without T2D. Participants with T2D tended to have lower CRF than participants without T2D (− 1.5 ml/kg/min, p = 0.094). Conclusion: CRF was inversely associated with liver fat content and insulin resistance. Participants with T2D had lower CRF than those without T2D, however, the difference was not statistically significant. Further longitudinal studies are required to elucidate the relationship between CRF and the progression of obesity-related diseases such as T2D. Registration: ACTRN12614001220651 (retrospectively registered on the 19th November 2014) and ACTRN12614000723684 (prospectively registered on the 8th July 2014)

Components of Antineutrino Emission in Nuclear Reactor

New ${\bar{\nu}_e},e$ scattering experiments aimed for sensitive searches of the ${\nu}_e$ magnetic moment and projects to explore small mixing angle oscillations at reactors call for a better understanding of the reactor antineutrino spectrum. Here we consider six components, which contribute to the total ${\bar{\nu}_e}$ spectrum generated in nuclear reactor. They are: beta decay of the fission fragments of $^{235}$U, $^{239}$Pu, $^{238}$U and $^{241}$Pu, decay of beta-emitters produced as a result of neutron capture in $^{238}$U and also due to neutron capture in accumulated fission fragments which perturbs the spectrum. For antineutrino energies less than 3.5 MeV we tabulate evolution of ${\bar{\nu}_e}$ spectra corresponding to each of the four fissile isotopes vs fuel irradiation time and their decay after the irradiation is stopped and also estimate relevant uncertainties. Small corrections to the ILL spectra are considered.Comment: LaTex 8 pages, 2 ps figure

The efficacy of exercise training for cutaneous microvascular reactivity in the foot in people with diabetes and obesity : secondary analyses from a randomized controlled trial

It is unclear if cutaneous microvascular dysfunction associated with diabetes and obesity can be ameliorated with exercise. We investigated the effect of 12-weeks of exercise training on cutaneous microvascular reactivity in the foot. Thirty-three inactive adults with type 2 diabetes and obesity (55% male, 56.1 +/- 7.9 years, BMI: 35.8 +/- 5, diabetes duration: 7.9 +/- 6.3 years) were randomly allocated to 12-weeks of either (i) moderate-intensity continuous training [50-60% peak oxygen consumption (VO2peak), 30-45 min, 3 d/week], (ii) low-volume high-intensity interval training (90% VO2peak, 1-4 min, 3 d/week) or (iii) sham exercise placebo. Post-occlusive reactive hyperaemia at the hallux was determined by laser-Doppler fluxmetry. Though time to peak flux post-occlusion almost halved following moderate intensity exercise, no outcome measure reached statistical significance (p > 0.05). These secondary findings from a randomised controlled trial are the first data reporting the effect of exercise interventions on cutaneous microvascular reactivity in the foot in people with diabetes. A period of 12 weeks of moderate-intensity or low-volume high-intensity exercise may not be enough to elicit functional improvements in foot microvascular reactivity in adults with type 2 diabetes and obesity. Larger, sufficiently powered, prospective studies are necessary to determine if additional weight loss and/or higher exercise volume is required