Development of octopus aquaculture: Rearing, handling and systems design for Octopus tetricus commercial aquaculture FRDC Project No 2009/206

The following document ‘Development of octopus aquaculture, rearing, handling and systems designs for Octopus tetricus commercial aquaculture’ contains protocols developed during the FRDC project 2009/206. These protocols encompass the most up-to-date rearing, handling and systems designs for Octopus tetricus commercial aquaculture. These protocols are the result of extensive research and development work carried out over the past four years by the Department of Fisheries, Western Australia and summarised in the final project report

Development of octopus aquaculture Final Report FRDC Project No 2009/206

During the past four years, the marine aquaculture group at the WA Fisheries and Marine Research Laboratories (WAFMRL) in collaboration with Occoculture Pty Ltd (subsidiary of Fremantle Octopus) and as a part of the FRDC project ‘Octopus Aquaculture Development’, investigated the potential of octopus aquaculture in Western Australia. Hatchery methods were developed in an attempt to close the life cycle. Advanced systems, rearing and feeding protocols were established for ranching Octopus tetricus juveniles

Investigating Red Knot Migration Ecology along the Georgia and South Carolina Coasts: Spring 2019 Season Summaries

The rufa subspecies of the Red Knot (Calidris canutus) has declined significantly in the past 35 years, leading to federal listing (US Fish and Wildlife Service Federal Register Vol. 79 No. 238, 2014a) under the Endangered Species Act in the United States (16 U.S.C. 1531 et. seq) and Canada (COSEWIC 2007, SARA 2007). Evidence for the decline is seen in long-term surveys of a major spring staging site (Dunne et al 1982, Clark et al. 1993, Niles et al. 2008) and the largest known over-wintering site (Morrison et al. 2004). In only 30 years, the estimated population has declined from 100,000-150,000 to possibly below 30,000 (Niles et al. 2007) leading some researchers to suggest the population is highly vulnerable to extinction (Baker et al. 2004). The determination of regional population estimates and identification of major stopover sites are considered to be the highest priority for the Georgia Department of Natural Resources State Wildlife Action Plan (2015), the Atlantic Flyway Shorebird Business Strategy (Winn et al. 2013), the US Shorebird Plan (Brown et al. 2001), the USFWS Red Knot Spotlight Species Action Plan (2010), and the Western Hemisphere Shorebird Reserve Network (WHSRN) Red Knot Conservation Plan for the Western Hemisphere (Niles et al. 2010a). The Georgia Department of Natural Resources State Wildlife Action Plan ranks the Red Knot as a high priority species (with state status of “Rare”) and ranks research of the Red Knot as one of the primary conservation actions needed within the state. A band resight program was initiated along the Georgia coastal barrier islands during the fall of 2011 and spring of 2013 and 2015-2016 giving baseline information for those seasons (Lyons et al. 2017, Smith et al. 2017). A trapping and tagging project was initiated in South Carolina in recent years, though there has been no systematic resight effort within the state. The patterns observed in both studies suggest that Red Knots are using the south-Atlantic through Delaware Bay in spring as an open network of staging areas. Recent tagging results in South Carolina have documented direct spring flights from South Carolina to James Bay, suggesting that the south-Atlantic stopovers are more important in the life-cycle of shorebirds than is currently thought. It is currently unknown what percentage of Red Knots use the direct flight strategy vs. stopping in Delaware Bay on their northbound migration. Winter resighting work conducted in South Carolina suggests that virtually no Red Knots wintering along the south-Atlantic use Delaware Bay in spring migration. Although movement and settlement “decisions” are likely influenced by foraging conditions throughout the network, investigations into the factors driving movements during migration is necessary to better understand Red Knot migration ecology. This is critical in developing appropriate stopover models and adaptive management tools for land managers. The Georgia Department of Natural Resources, the South Carolina Department of Natural Resources, the US Fish and Wildlife Service, and The Center for Conservation Biology will initiate a Red Knot tagging and resighting program along the Atlantic Coast of Georgia during the spring of 2019 to be paired with the ongoing programs within Delaware Bay and elsewhere along the flyway. This project will provide critical data that will be used to analyze the ongoing questions regarding Red Knot habitat choice decision making in the south Atlantic Coast in spring. A large percentage (3-6%) of Red Knots have been previously captured and tagged with unique 2 to 3 digits alpha-numeric bands. This marked population allows for mark-resight studies of migratory populations of Red Knots with no capturing involved. A total of 27,356 Red Knots were detected during daily surveys in spring 2019 along the Georgia and South Carolina Coasts; of those, 4,917 were scanned for flags, and 315 individually banded Red Knots were resighted a total of 523 times from within the spring migrant population. A total of 71 marked to unmarked ratios were recorded during the field season, with an average of 3.6% of Red Knots individually marked over the course of the spring. The rough estimate for the total number of knots cycling through during the spring season is estimated superpopulation size for the spring 2019 season is 8,750 (3.6% of birds tagged and 315 individuals recorded). The Georgia Coast is a major stopover area annually for rufa Red Knots in spring migration and in certain years in fall migration. The superpopulation utilizing the coast in fall migration can exceed 23,000 birds (Lyons et al. 2017) and the rough estimate of spring migration superpopulation from this study is approximately 8,750 birds. The total estimated population of rufa Red Knots is 42,000 birds (Andres et al. 2012), suggesting that a high percentage of rufa knots are using the Georgia Coast in spring and in some years fall migration. There appears to be less variation in spring migration superpopulations between years than in fall migration, suggesting a more stable (but less abundant) food source for spring migrants

A case of AML characterized by a novel t(4;15)(q31;q22) translocation that confers a growth-stimulatory response to retinoid-based therapy

Here we report the case of a 30-year-old woman with relapsed acute myeloid leukemia (AML) who was treated with all-transretinoic acid (ATRA) as part of investigational therapy (NCT02273102). The patient died from rapid disease progression following eight days of continuous treatment with ATRA. Karyotype analysis and RNA-Seq revealed the presence of a novel t(4;15)(q31;q22) reciprocal translocation involving theTMEM154andRASGRF1genes. Analysis of primary cells from the patient revealed the expression ofTMEM154-RASGRF1mRNA and the resulting fusion protein, but no expression of the reciprocalRASGRF1-TMEM154fusion. Consistent with the response of the patient to ATRA therapy, we observed a rapid proliferation of t(4;15) primary cells following ATRA treatment ex vivo. Preliminary characterization of the retinoid response of t(4;15) AML revealed that in stark contrast to non-t(4;15) AML, these cells proliferate in response to specific agonists of RARα and RARγ. Furthermore, we observed an increase in the levels of nuclear RARγ upon ATRA treatment. In summary, the identification of the novel t(4;15)(q31;q22) reciprocal translocation opens new avenues in the study of retinoid resistance and provides potential for a new biomarker for therapy of AML

Coupled land use and ecological models reveal emergence and feedbacks in socio-ecological systems

Acknowledgements: This work was supported by an EPSRC Doctoral Training Centre grant (EP/G03690X/1). Supplementary material (Appendix ECOG‐04039 at ). Appendix 1.Peer reviewedPublisher PD

Bulk flows in Virasoro minimal models with boundaries

The behaviour of boundary conditions under relevant bulk perturbations is studied for the Virasoro minimal models. In particular, we consider the bulk deformation by the least relevant bulk field which interpolates between the mth and (m-1)st unitary minimal model. In the presence of a boundary this bulk flow induces an RG flow on the boundary, which ensures that the resulting boundary condition is conformal in the (m-1)st model. By combining perturbative RG techniques with insights from defects and results about non-perturbative boundary flows, we determine the endpoint of the flow, i.e. the boundary condition to which an arbitrary boundary condition of the mth theory flows to.Comment: 34 pages, 6 figures. v4: Typo in fig. 2 correcte

Emerging Opportunities for Landscape Ecological Modelling

Landscape ecological modelling provides a vital means for understanding the interactions between geographical, climatic, and socio-economic drivers of land-use and the dynamics of ecological systems. This growing field is playing an increasing role in informing landscape spatial planning and management. Here, we review the key modelling approaches that are used in landscape modelling and in ecological modelling. We identify an emerging theme of increasingly detailed representation of process in both landscape and ecological modelling, with complementary suites of modelling approaches ranging from correlative, through aggregated process based approaches to models with much greater structural realism that often represent behaviours at the level of agents or individuals. We provide examples of the considerable progress that has been made at the intersection of landscape modelling and ecological modelling, while also highlighting that the majority of this work has to date exploited a relatively small number of the possible combinations of model types from each discipline. We use this review to identify key gaps in existing landscape ecological modelling effort and highlight emerging opportunities, in particular for future work to progress in novel directions by combining classes of landscape models and ecological models that have rarely been used together

SHAPE-directed Discovery of Potent shRNA Inhibitors of HIV-1

The RNA interference (RNAi) pathway can be exploited using short hairpin RNAs (shRNAs) to durably inactivate pathogenic genes. Prediction of optimal target sites is notoriously inaccurate and current approaches applied to HIV-1 show weak correlations with virus inhibition. In contrast, using a high-content model for disrupting pre-existing intramolecular structure in the HIV-1 RNA, as achievable using high-resolution SHAPE (selective 2′-hydroxyl acylation analyzed by primer extension) chemical probing information, we discovered strong correlations between inhibition of HIV-1 production in a quantitative cell-based assay and very simple thermodynamic features in the target RNA. Strongest inhibition occurs at RNA target sites that both have an accessible “seed region” and, unexpectedly, are structurally accessible in a newly identified downstream flanking sequence. We then used these simple rules to create a new set of shRNAs and achieved inhibition of HIV-1 production of 90% or greater for up to 82% of designed shRNAs. These shRNAs inhibit HIV-1 replication in therapy-relevant T cells and show no or low cytotoxicity. The remarkable success of this straightforward SHAPE-based approach emphasizes that RNAi is governed, in significant part, by very simple, predictable rules reflecting the underlying RNA structure and illustrates principles likely to prove broadly useful in understanding transcriptome-scale biological recognition and therapeutics involving RNA

Actors of the common interest? The Brussels offices of the regions.

The absence of a formal place in representative democracy at EU level casts sub-national authorities more as actors of EU participatory democracy. Where they have specific interests to pursue their Brussels offices act in the same way as lobbyists, but public authorities are also capable of acting on broader interest sets. This analysis is geared to understanding variation in the extent to which the diversely constituted Brussels offices of the regions can act on a broad spectrum of civil society interests, and thus have potential as actors of European integration in connecting civil society with EU institutions. Differences in the orientation of offices towards either highly defined or broad agendas can be conceived in qualified principal-agent terms, in which the autonomy of offices to develop activities is the critical explanatory factor. This autonomy can be derived more from the structure of principals and from degrees of purpose they have than from asymmetries of power between principals and agents, which in turn can be drawn from typologies of degrees of devolved authority present in different member states. It predicts that territorial offices from member states with medium degrees of devolved authority have the greatest potential to act on a broad range of civil society oriented interests