Morphometric and molecular analysis of two distinct forms of Pseudocrossidium crinitum

Two distinct forms of Pseudocrossidium crinitum (Bryophyta) exist- a form with a white hair point on the leaf, and one with a yellow hair point on the leaf. The white and yellow forms exist in similar areas, but the yellow hair point form is found on its own in arid areas. In this paper we analyse 16 morphometric variables and chloroplast and nuclear DNA molecular information to determine whether Pseudocrossidium crinitum should be separated into two species based on morphological differences. We use ANOVA, Principal components analysis and cluster analysis to analyse the morphometric data. Four significantly different morphological differences were found between the two forms. Five haplotypes were revealed from 8 samples, with only one haplotype shared amongst them. Two samples from the Cedarburg exhibit highly different DNA to the rest of the samples. Although genetic sampling was not large enough on which to base significant conclusions, we find that the two forms - are morphologically differentiated enough to separate Pseudocrossidium crinitum into two species

Hillary L. Chute. Disaster Drawn: Visual Witness, Comics, and Documentary Form. Cambridge: Harvard UP, 2016.

Review of Hillary L. Chute. Disaster Drawn: Visual Witness, Comics, and Documentary Form. Cambridge: Harvard UP, 2016

Development of a Coding Instrument to Assess the Quality and Content of Anti-Tobacco Video Games

Previous research has shown the use of electronic video games as an effective method for increasing content knowledge about the risks of drugs and alcohol use for adolescents. Although best practice suggests that theory, health communication strategies, and game appeal are important characteristics for developing games, no instruments are currently available to examine the quality and content of tobacco prevention and cessation electronic games. This study presents the systematic development of a coding instrument to measure the quality, use of theory, and health communication strategies of tobacco cessation and prevention electronic games. Using previous research and expert review, a content analysis coding instrument measuring 67 characteristics was developed with three overarching categories: type and quality of games, theory and approach, and type and format of messages. Two trained coders applied the instrument to 88 games on four platforms (personal computer, Nintendo DS, iPhone, and Android phone) to field test the instrument. Cohen's kappa for each item ranged from 0.66 to 1.00, with an average kappa value of 0.97. Future research can adapt this coding instrument to games addressing other health issues. In addition, the instrument questions can serve as a useful guide for evidence-based game development.Food and Drug Administration (FDA) Center for Tobacco ProductsNational Cancer Institute (NCI) Office of Communication and EducationCommunication Studie

Grey and white matter differences in Chronic Fatigue Syndrome : A voxel-based morphometry study

Conflicts of interest and source of funding The authors declare no conflicts of interest. This research was funded by the Medical Research Council (MR/J002712/1). AF is supported by Research Capability Funding from the Newcastle upon Tyne Hospitals NHS Foundation Trust and the Northumberland, Tyne and Wear NHS Foundation Trust.Peer reviewedPublisher PD

Operationalising Human Security in the Contemporary Operating Environment: Proposing Population Intelligence (POPINT)

Drawing upon primary research funded by the UK Defence and Security Accelerator (DASA), this article is about using data analytics and artificial intelligence (AI) for operationalising human security in the contemporary operating environment. The idea of human security has gained much traction in the international community since its introduction in a 1994 United Nations Development Programme (UNDP) report and has more recently become a military concern. Yet, the core tenets of this idea remain contested, and the military role in support of human security remains an open question. Nonetheless, the concurrent increase in Open Data and AI does give rise to new opportunities to understand the various human security concerns. In response, DASA funded Projects SOLEBAY and HAMOC to research these concerns and the possibilities of data analytics for human security. Drawing on the research findings, we propose the idea of Population Intelligence (POPINT) as a new intelligence discipline to operationalise human security

Investigating the role of Wnt/Planar cell polarity (PCP) in Neuromesodermal Progenitors (NMPs)

Neuromesodermal progenitors (NMPs) are bipotent progenitors, located at the caudal end of the embryo and are essential for axis formation. These stem cell-like progenitors possess the ability to self-renew and differentiate to both mesodermal and neural lineages, such as skeletal muscle and spinal cord derivatives. These progenitors arise at E8.5 and are localised in the caudal lateral epiblast (CLE), a posterior region of the embryo near the primitive streak. Later in development, they reside in the tail bud until cessation of axial elongation at E13.5. Throughout these stages NMPs are characteristically marked by co-expression of T(Bra) (Brachyury) and Sox2. This characteristic is also present in in vitro NMPs, which can be derived from Epiblast Stem Cells (EpiSCs) through treatment with Wnt/β-catenin signalling agonists and Fgf2, which simulates their in vivo environment. Protein and mRNA profiling of NMPs and mutant phenotypes in vivo supports the hypothesis that a non-canonical Wnt pathway, the Wnt/Planar Cell Polarity pathway (PCP) could be involved in NMP fate decision and/or maintenance. This thesis focuses on understanding more about the role of PCP by aiming to identify the spatio-temporal profile of Wnt/PCP pathway components in NMP regions during axial elongation, as well as determining its role in NMP behaviour through manipulation of this pathway via in vivo and in vitro assays Employing in situ hybridisation and immunohistochemistry techniques, key Wnt/PCP components, including Pk1, Vangl2 and Ptk7, were confirmed to be present in in vivo and in vitro NMPs, thus, providing strong evidence that Wnt/PCP may be involved regulating NMP behaviour. Disruption of Wnt/PCP signalling through overexpression of Wnt/PCP components was tested in refined in vivo and in vitro assays. Overexpression of Vangl2 and Ptk7, but not Pk1 in NMPs regions in vivo resulted in loss of contribution to neural lineages, as well as lower contribution to NMP regions themselves. Similarly, Wnt/PCP components were disrupted in vitro through generation of dox-inducible overexpression cells lines for Wnt/PCP components. These lines were used to generate NMPs from an optimised novel alternative source Epiblast-Like Cells (EpiLCs), however no clear affect to lineage was observed. Overall this work has successfully advanced our knowledge of Wnt/PCP mediated control of NMP differentiation and maintenance, and provided a finer grained description of the relationships between them

Distinguishing offshore bird hunting from beach scavenging in archaeological contexts: the value of modern beach surveys

Determining whether seabirds recovered from coastal shell middens were obtained via active hunting or scavenging of beached carcasses is a challenge for archaeologists. Traditional methods have included analyzing skeletal part frequencies, abundance, age profiles, and contextual evidence. The assumption has been made, based on limited biological data, that an assemblage of carcasses scavenged from the beach will have more wing elements, and fewer legs and heads. Few studies, however, have embraced modern beaching data to verify this assumption and assess the potential faunal resources available for scavenging. We analyze the skeletal part representation of modern beached birds observed by the Coastal Observation and Seabird Survey Team (COASST), comparing the COASST dataset to two idealized hypotheses used by archaeologists: the human scavenging hypothesis (wings only are recovered, while heads and legs are absent) and the human hunting hypothesis (all body parts are found in equal proportions). Finally, we apply these results to analysis of the bird remains from the Minard site (45-GH-15), a late Holocene coastal site in Grays Harbor, Washington. We find that contemporary beached bird data are closer to replicating the human hunting hypothesis as compared to the human scavenging hypothesis, as \u3e75% of the 19,599 carcasses in the COASST dataset had a combination of head, wings and legs. This result, and the similarity in taxonomic distribution between our contemporary beached bird data and Minard assemblage, suggests that indigenous peoples may have used scavenging as a viable means of resource acquisition in the past. Use of contemporaneous beached bird data may provide zooarchaeology with a statistically defensible baseline of information on the phenology, abundance and condition of bird carcasses

Low prevalence search for cancers in mammograms : evidence using laboratory experiments and computer aided detection

People miss a large proportion of targets when they only appear rarely. This Low Prevalence (LP) Effect could lead to serious consequences if it occurred in the real world task of searching for cancers in mammograms. Using a novel mammogram search task, we asked participants to search for a pre-specified cancer (Experiments 1-2) or a range of masses (Experiments 3-5) under high or low prevalence conditions. Experiment 1 showed that an LP Effect occurred using these stimuli. Experiment 2 tested an over-reliance hypothesis and showed that the use of Computer Aided Detection (CAD) led to fewer missed cancers with a valid CAD prompt yet, a large proportion of cancers were missed when CAD was incorrect. Experiment 3 - 5 showed that false alarms also increased when searching for a range of masses and that CAD reduced miss errors when it correctly cued the target but increased miss errors and false alarms when it did not. Furthermore, when a mass fell outside the CAD prompt it was more likely to be misidentified. No LP Effect was observed with the addition of CAD when people were asked to search for a range of targets. Theories and implications for mammogram search are discussed

Ultraviolet Emission Lines in Young Low Mass Galaxies at z~2: Physical Properties and Implications for Studies at z>7

We present deep spectroscopy of 17 very low mass (M* ~ 2.0x10^6 Msun to 1.4x10^9 Msun) and low luminosity (M_UV ~ -13.7 to -19.9) gravitationally lensed galaxies in the redshift range z~1.5-3.0. Deep rest-frame ultraviolet spectra reveal large equivalent width emission from numerous lines (NIV], OIII], CIV, Si III], CIII]) which are rarely seen in individual spectra of more massive star forming galaxies. CIII] is detected in 16 of 17 low mass star forming systems with rest-frame equivalent widths as large as 13.5 Angstroms. Nebular CIV emission is present in the most extreme CIII] emitters, requiring an ionizing source capable of producing a substantial component of photons with energies in excess of 47.9 eV. Photoionization models support a picture whereby the large equivalent widths are driven by the increased electron temperature and enhanced ionizing output arising from metal poor gas and stars, young stellar populations, and large ionization parameters. The young ages implied by the emission lines and continuum SEDs indicate that the extreme line emitters in our sample are in the midst of a significant upturn in their star formation activity. The low stellar masses, blue UV colors, and large sSFRs of our sample are similar to those of typical z>6 galaxies. Given the strong attenuation of Ly-alpha in z>6 galaxies we suggest that CIII] is likely to provide our best probe of early star forming galaxies with ground-based spectrographs and one of the most efficient means of confirming z>10 galaxies with the James Webb Space Telescope.Comment: 22 pages, 8 figures, accepted for publication in MNRA