Adjustment to Cancer among Patients with Breast Cancer Hospitalized in the Day Care Oncology Ward

Introduction: Breast cancer is one of the most common malignant tumours affecting women. The treatment often involves the surgical removal of the neoplastic lesion or even the resection of the whole breast. The disease affects virtually every sphere of a woman's life. The aim of the work was to learn strategies for coping with pain on the example of patients of the Neurological Hospital of the City Hospital named after John Paul II in Bielsko-Biała. Materials and methods: The study involved 100 patients with breast cancer following oncological surgery who were hospitalized in the oncology ward of the Beskid Oncology Centre of the Town Hospital in Bielsko-Biała. The research was carried out from March to December 2017. The following research tools were used: self-inquiry questionnaire and the Mini-Mac Mental Adjustment Scale for cancer. Findings: While analysing the ways of coping with cancer, it was shown that the constructive style definitely dominates along with the high intensity of the positive re-evaluation strategy, the fighting spirit. The destructive style, however, scored low among the respondents. Conclusions: The patients showed an attitude leaning towards cancer adjustment. Most women had a positive attitude towards their disease

Developmnet of Q+T heat treatment technology of 41CrAlMo7S steel

Tato bakalářská práce se zabývá technologií zušlechťování oceli 41CrAlMo7S na zařízení s linkou a vybavením, které je nedílnou součástí komplexního elektrotermického systému určeného k zušlechťování tyčí. V práci jsou v hlavních bodech popsány technologické postupy zpracování a výroby ocelových tyčí, jejich tepelné zpracování, zušlechťovací procesy, vyhodnocení mechanických a strukturálních vlastností, popisy vzorků a návrhy na optimální řešení zušlechtění oceli v provozních podmínkách. Teoretické i praktické poznatky a zkušenosti byly následně využity v pracovním procesu.This bachelor thesis deals with quenching and tempering of steel 41CrAlMo7S on the device to which belongs line with equipment, which is an integral part of complex electro-thermic system intended for finishing bars. In the main points in bachelor thesis are described technological processes and production of steel bars/rods, heat treatment, refining processes, evaluation of mechanical and structural properties, description of samples, proposals for optimal solutions of steel in operating conditions. Theoretical and practical knowledge and experience were subsequently used in the work process.636 - Katedra materiálového inženýrstvívelmi dobř

Mitotane Inhibits Proliferation and Affects Mitochondrial Metabolism in Human Breast, Lung and Colon Cancer Cell Lines

<p>Mitotane acts specifically on adrenocortical tissue and is used as a standard treatment in adrenocortical cancer. The exact mechanism of its action remains unknown, despite its clinical use for more than 60 years. Recently it has been suggested that in human adrenocortical cancer cells, mitotane alters mitochondrial respiratory chain activity by inducing cytochrome c oxidase defect. The aim of the study was to assess the effect of Mitotane on proliferation, apoptosis and mitochondrial metabolism in human breast (MCF7), lung (H1975) and colon (HKe-3) cancer cell lines and compare it with our previous results in human adrenocortical cancer (H295R). The proliferation rate of cells was assessed by the resazurin assay. The apoptosis induction was determined by the caspase-3-like activity assay. Changes in expression of the 84 genes involved in mitochondrial metabolism (Complex I: NADH-Coenzyme Q Reductase; Complex II: Succinate-Coenzyme Q Reductase; Complex III: Coenzyme Q-Cytochrome c Reductase; Complex IV: Cytochrome c Oxidase; Complex V: ATP Synthase) were assessed using Mitochondrial Energy Metabolism Plus PCR Arrays, Qiagen. Optimum cytotoxic effects of Mitotane were observed after 24 and 48 hours incubation for H295R cells and HKe-3, MCF7, H1975; respectively. In the H295R cell line a 10uM concentration of Mitotane resulted in 27.0±0.9% cytotoxicity, whereas in HKe-3, MCF7 and H1975 cell lines concentration of 40uM caused cytotoxicity of 48.5±5.2%; 40.2±2.5% and 28.6±2.1%, respectively. 100% cytotoxicity was achieved at a concentration of 20uM for H295R and 100uM for HKe-3, MCF7 and H1975. Cytotoxic effects were negatively influenced by the number of cells/well in H295R, HKe-3, and H1975 but not in MCF7. Although Mitotane had a strong cytotoxic effect on all tested cell lines, caspase-3 activity was induced only in H295R cell line. Expression levels of several genes involved in mitochondrial metabolism was changed by Mitotane; however different genes were affected in different cell lines. Of 84 genes investigated, mRNA levels were decreased/increased by >2 folds in 18/4; 10/29; 6/7 and 28/2 in H295R, HKe-3, MCF7 and H1975, respectively. Mitotane exhibits anti-proliferative activity in breast, colon and lung cancer cell lines. It affects mitochondrial metabolism regardless of the type of cancer and hence his cytotoxic effect is not specific to the adrenal cortex only. <br><em>Mitotane Inhibits Proliferation and Affects Mitochondrial Metabolism in Human Breast, Lung and Colon Cancer Cell Lines</em>. Available from: <a href="https://www.researchgate.net/publication/274961069_Mitotane_Inhibits_Proliferation_and_Affects_Mitochondrial_Metabolism_in_Human_Breast_Lung_and_Colon_Cancer_Cell_Lines">https://www.researchgate.net/publication/274961069_Mitotane_Inhibits_Proliferation_and_Affects_Mitochondrial_Metabolism_in_Human_Breast_Lung_and_Colon_Cancer_Cell_Lines</a> [accessed Apr 14, 2015].</p> <p> </p

Mitotane Inhibits Proliferation and Affects Mitochondrial Metabolism in Human Breast, Lung and Colon Cancer Cell Lines _ Tumorigenesis, Metastasis, and Therapies for Cancer.pdf

Mitotane acts specifically on adrenocortical tissue and is used as a standard treatment in adrenocortical cancer. The exact mechanism of its action remains unknown, despite its clinical use for more than 60 years. Recently it has been suggested that in human adrenocortical cancer cells, mitotane alters mitochondrial respiratory chain activity by inducing cytochrome c oxidase defect.<br><br>The aim of the study was to assess the effect of Mitotane on proliferation, apoptosis and mitochondrial metabolism in human breast (MCF7), lung (H1975) and colon (HKe-3) cancer cell lines and compare it with our previous results in human adrenocortical cancer (H295R).<br><br>The proliferation rate of cells was assessed by the resazurin assay. The apoptosis induction was determined by the caspase-3-like activity assay. Changes in expression of the 84 genes involved in mitochondrial metabolism (Complex I: NADH-Coenzyme Q Reductase; Complex II: Succinate-Coenzyme Q Reductase; Complex III: Coenzyme Q-Cytochrome c Reductase; Complex IV: Cytochrome c Oxidase; Complex V: ATP Synthase) were assessed using Mitochondrial Energy Metabolism Plus PCR Arrays, Qiagen.<br><br>Optimum cytotoxic effects of Mitotane were observed after 24 and 48 hours incubation for H295R cells and HKe-3, MCF7, H1975; respectively. In the H295R cell line a 10uM concentration of Mitotane resulted in 27.0±0.9% cytotoxicity, whereas in HKe-3, MCF7 and H1975 cell lines concentration of 40uM caused cytotoxicity of 48.5±5.2%; 40.2±2.5% and 28.6±2.1%, respectively. 100% cytotoxicity was achieved at a concentration of 20uM for H295R and 100uM for HKe-3, MCF7, and H1975. Cytotoxic effects were negatively influenced by the number of cells/well in H295R, HKe-3, and H1975 but not in MCF7. Although Mitotane had a strong cytotoxic effect on all tested cell lines, caspase-3 activity was induced only in H295R cell line. Expression levels of several genes involved in mitochondrial metabolism were changed by Mitotane; however, different genes were affected in different cell lines. Of 84 genes investigated, mRNA levels were decreased/increased by >2 folds in 18/4; 10/29; 6/7 and 28/2 in H295R, HKe-3, MCF7, and H1975, respectively.<br><br>Mitotane exhibits antiproliferative activity in breast, colon and lung cancer cell lines. It affects mitochondrial metabolism regardless of the type of cancer and hence his cytotoxic effect is not only accurate to the adrenal cortex