Application of the indirect fluorescent antibody assay in the study of malaria infection in the Yangtze River Three Gorges Reservoir, China

<p>Abstract</p> <p>Background</p> <p>China Yangtze Three Gorges Project (TGP) is one of the biggest construction projects in the world. The areas around the Three Gorge Dam has a history of tertian malaria and subtertian malaria epidemic, but there are no overall data about malaria epidemics before the completion of the project. The objective of this study was to get a reliable baseline on malaria infection in the Yangtze River Three Gorges reservoir area and to provide reference data for future studies about the impact of the project on malaria epidemics.</p> <p>Methods</p> <p>Two surveys of malaria infection were carried out in area, at six-month intervals in May and October 2008. About 3,600 dual specimens blood film samples for parasite diagnosis and filter paper blood spots for serology (using the immunofluorescence antibody test) were collected from the general population, including school populations, whenever possible.</p> <p>Results</p> <p>The overall percentage of positive response of the same population during post-transmission periods was about twice (1.40/0.72) of that in pre-transmission. Positive individuals under 15 years of age were detected in all the localities.</p> <p>Conclusion</p> <p>A certain extent of malaria infection existed in this area. Additional studies are needed to determine the length of malaria experience, and chemotherapeutic intervention as well as the distribution of main vectors for transmission in this area.</p

Comparative Evaluation of Light-Trap Catches, Electric Motor Mosquito Catches and Human Biting Catches of Anopheles in the Three Gorges Reservoir

The mosquito sampling efficiency of light-trap catches and electric motor mosquito catches were compared with that of human biting catches in the Three Gorges Reservoir. There was consistency in the sampling efficiency between light-trap catches and human biting catches for Anopheles sinensis (r = 0.82, P<0.01) and light-trap catches were 1.52 (1.35–1.71) times that of human biting catches regardless of mosquito density (r = 0.33, P>0.01), while the correlation between electric motor mosquito catches and human biting catches was found to be not statistically significant (r = 0.43, P>0.01) and its sampling efficiency was below that of human biting catches. It is concluded that light-traps can be used as an alternative to human biting catches of Anopheles sinensis in the study area and is a promising tool for sampling malaria vector populations

China–Africa cooperation initiatives in malaria control and elimination

Malaria has affected human health globally with a significant burden of disease, and also has impeded social and economic development in the areas where it is present. In Africa, many countries have faced serious challenges in controlling malaria, in part due to major limitations in public health systems and primary health care infrastructure. Although China is a developing country, a set of control strategies and measures in different local settings have been implemented successfully by the National Malaria Control Programme over the last 60 years, with a low cost of investment. It is expected that Chinese experience may benefit malaria control in Africa. This review will address the importance and possibility of China–Africa collaboration in control of malaria in targeted African countries, as well as how to proceed toward the goal of elimination where this is technically feasible.China UK Global Health Support Programme (grant no.GHSP-CS-OP1, OP2, OP3).http://www.elsevier.com/books/book-series/advances-in-parasitologyhb201

Cell transcriptomic atlas of the non-human primate Macaca fascicularis.

Studying tissue composition and function in non-human primates (NHPs) is crucial to understand the nature of our own species. Here we present a large-scale cell transcriptomic atlas that encompasses over 1 million cells from 45 tissues of the adult NHP Macaca fascicularis. This dataset provides a vast annotated resource to study a species phylogenetically close to humans. To demonstrate the utility of the atlas, we have reconstructed the cell-cell interaction networks that drive Wnt signalling across the body, mapped the distribution of receptors and co-receptors for viruses causing human infectious diseases, and intersected our data with human genetic disease orthologues to establish potential clinical associations. Our M. fascicularis cell atlas constitutes an essential reference for future studies in humans and NHPs.We thank W. Liu and L. Xu from the Huazhen Laboratory Animal Breeding Centre for helping in the collection of monkey tissues, D. Zhu and H. Li from the Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory) for technical help, G. Guo and H. Sun from Zhejiang University for providing HCL and MCA gene expression data matrices, G. Dong and C. Liu from BGI Research, and X. Zhang, P. Li and C. Qi from the Guangzhou Institutes of Biomedicine and Health for experimental advice or providing reagents. This work was supported by the Shenzhen Basic Research Project for Excellent Young Scholars (RCYX20200714114644191), Shenzhen Key Laboratory of Single-Cell Omics (ZDSYS20190902093613831), Shenzhen Bay Laboratory (SZBL2019062801012) and Guangdong Provincial Key Laboratory of Genome Read and Write (2017B030301011). In addition, L.L. was supported by the National Natural Science Foundation of China (31900466), Y. Hou was supported by the Natural Science Foundation of Guangdong Province (2018A030313379) and M.A.E. was supported by a Changbai Mountain Scholar award (419020201252), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16030502), a Chinese Academy of Sciences–Japan Society for the Promotion of Science joint research project (GJHZ2093), the National Natural Science Foundation of China (92068106, U20A2015) and the Guangdong Basic and Applied Basic Research Foundation (2021B1515120075). M.L. was supported by the National Key Research and Development Program of China (2021YFC2600200).S

Aggregation-Induced Emission (AIE), Life and Health

Light has profoundly impacted modern medicine and healthcare, with numerous luminescent agents and imaging techniques currently being used to assess health and treat diseases. As an emerging concept in luminescence, aggregation-induced emission (AIE) has shown great potential in biological applications due to its advantages in terms of brightness, biocompatibility, photostability, and positive correlation with concentration. This review provides a comprehensive summary of AIE luminogens applied in imaging of biological structure and dynamic physiological processes, disease diagnosis and treatment, and detection and monitoring of specific analytes, followed by representative works. Discussions on critical issues and perspectives on future directions are also included. This review aims to stimulate the interest of researchers from different fields, including chemistry, biology, materials science, medicine, etc., thus promoting the development of AIE in the fields of life and health

Optimization Model of Close Supply Chain of Green Agricultural Products

Close supply chain of green agricultural products is defined, consisting of the supplier, manufacturer, distributor, retailer and consumer (customer). Cost structure of distributor is analyzed, mainly including the order activity-based cost, the inventory cost and the supply transportation cost of agricultural products in upstream and downstream enterprises. Based on this, conceptual model of the close supply chain of green agricultural products is established. According to the idea of dynamic planning, optimization model of close supply chain of green agricultural products at four stages is also set up. In other words, under the constraint condition of supply chain and the uncertainty of sales volume, optimization of the sum of inventory cost, supply transportation cost and order activity-based cost in supply chain at retailer, distributor, manufacturer and suppliers stages is studied, which provides references for the analysis and decision-making of supply chain management

Operating Analysis of the Closed Supply Chain of Green Agricultural Products Based on Logistics Center

This thesis gives the overview of concept and constitution of the closed supply chain of green agricultural products on logistics center, and the necessity of regarding logistics center as core enterprises. Meanwhile, it analyzes the function of logistic center of agricultural products, namely functions of exchange, collection, distribution, storage and transportation. It also poses the prerequisites of logistics center. The logistics center of agricultural products must have stable profit expectancy, prominent scale, strong appealing power, capacity of bearing market risk and basic thoughts of supply chain management. The functional design of the closed supply chain of green agricultural products has been discussed in 5 aspects, namely logistics center, production base, provides for means of production, retailer and consumer. The operating thoughts of the closed supply chain of green agricultural products based on logistics center are put forward on the basis of the research of operating objective and service target of supply chain as follows: first, based on local principal agricultural products, the logistics center mainly distributes the massed agricultural products; second, it is necessary to choose appropriate strategic cooperative partners to sign contract; third, the operating procedures of supply chain entails bringing in professional managerial talents of supply chain; finally, the relationships of supply chain should be maintained

Aptamer‐functionalized field‐effect transistor biosensors for disease diagnosis and environmental monitoring

Abstract Nano‐biosensors that are composed of recognition molecules and nanomaterials have been extensively utilized in disease diagnosis, health management, and environmental monitoring. As a type of nano‐biosensors, molecular specificity field‐effect transistor (FET) biosensors with signal amplification capability exhibit prominent advantages including fast response speed, ease of miniaturization, and integration, promising their high sensitivity for molecules detection and identification. With intrinsic characteristics of high stability and structural tunability, aptamer has become one of the most commonly applied biological recognition units in the FET sensing fields. This review summarizes the recent progress of FET biosensors based on aptamer functionalized nanomaterials in medical diagnosis and environmental monitoring. The structure, sensing principles, preparation methods, and functionalization strategies of aptamer modified FET biosensors were comprehensively summarized. The relationship between structure and sensing performance of FET biosensors was reviewed. Furthermore, the challenges and future perspectives of FET biosensors were also discussed, so as to provide support for the future development of efficient healthcare management and environmental monitoring devices

Operating Analysis of the Closed Supply Chain of Green Agricultural Products Based on Logistics Center

This thesis gives the overview of concept and constitution of the closed supply chain of green agricultural products on logistics center, and the necessity of regarding logistics center as core enterprises. Meanwhile, it analyzes the function of logistic center of agricultural products, namely functions of exchange, collection, distribution, storage and transportation. It also poses the prerequisites of logistics center. The logistics center of agricultural products must have stable profit expectancy, prominent scale, strong appealing power, capacity of bearing market risk and basic thoughts of supply chain management. The functional design of the closed supply chain of green agricultural products has been discussed in 5 aspects, namely logistics center, production base, provides for means of production, retailer and consumer. The operating thoughts of the closed supply chain of green agricultural products based on logistics center are put forward on the basis of the research of operating objective and service target of supply chain as follows: first, based on local principal agricultural products, the logistics center mainly distributes the massed agricultural products; second, it is necessary to choose appropriate strategic cooperative partners to sign contract; third, the operating procedures of supply chain entails bringing in professional managerial talents of supply chain; finally, the relationships of supply chain should be maintained.Closed supply chain, Operating mode, Logistics center, China, Agribusiness,

Optimization Model of Close Supply Chain of Green Agricultural Products

Close supply chain of green agricultural products is defined, consisting of the supplier, manufacturer, distributor, retailer and consumer (customer). Cost structure of distributor is analyzed, mainly including the order activity-based cost, the inventory cost and the supply transportation cost of agricultural products in upstream and downstream enterprises. Based on this, conceptual model of the close supply chain of green agricultural products is established. According to the idea of dynamic planning, optimization model of close supply chain of green agricultural products at four stages is also set up. In other words, under the constraint condition of supply chain and the uncertainty of sales volume, optimization of the sum of inventory cost, supply transportation cost and order activity-based cost in supply chain at retailer, distributor, manufacturer and suppliers stages is studied, which provides references for the analysis and decision-making of supply chain management.Close supply chain, Operation optimization, Green agricultural products, China, Industrial Organization, Marketing,