Fruit thinning in organic apple growing with optimized timing and combination strategies including (new) natural spray products and mechanical rope-devices

The aim of our study was to find new thinning methods for organic apple production able to fulfil the standards of Bio-Suisse, the main label organisation of the organic food sector in Switzerland. The 14 trials reported were carried out during flowering period in 2003 and 2004 on the cultivars Pinova, Gala, Maigold, Elstar, Golden Delicious and Otava at different sites. The results confirm the good efficiency of mechanical thinning with the rope thinner and the thinning potential of vinasse, a by-product of molasses. Different vinasse products were tested (K-Vinasse, light-vinasse, N-Vinasse) and it was observed that those containing less nitrogen had a minor thinning effect. To avoid phytotoxicity on leaves, the concentration of N-Vinasse should not exceed 10 %. It seems that for Gala higher concentrations are needed, 7.5-10%, whereas with Maigold the efficacy of N-Vinasse was the same with the 2.5 % concentration as with 5 % and 10 %. On the other hand, partly good results were obtained when N-Vinasse was over-concentrated to 12 %, applied at warm weather and rinsed with clear water 4-6 hours later to avoid the phyto-toxic effect (“burn & rinse” method). Corn Oil, a commercial product from New Zealand, gave encouraging results. Lime sul-phur didn’t effectuate a significantly better thinning effect. The best method tested (fruit set reduction by 44 % and 26 % higher flower bud set next year) was achieved with a combination of rope thinner and N-Vinasse: rope thinner to thin the peripheral branches and to exert a physiological stress; and N-Vinasse to thin the inner parts of the canopy that the ropes can’t reach. Other tested products did not show satisfying effects. Trials also confirmed the importance of applying these thinning agents at least two times, at the appropriate phenological stage of flowering period (F,) F2 (full bloom) and G (= F2 of the one-year old shoots). Hitting also the flowers of the one-year old shoots is important to break bi-annual bearing. Pollen tube growth was assessed by fluorescence microscopy. Almost complete inhibition of pollen tube germina-tion was observed with Lime Sulphur and over-concentrated N-Vinasse (12 %) whereas with N-Vinasse at 5 % inhibition was 50 % less compared to untreated styles. Some contradictory results between different trials we ascribe to the air temperature during and after the treatments. It seems that treatments with N-Vinasse should be carried out at warm and sunny days above 16, better 18-20 deg. C. to maximize phytotoxicity on the styles; whereas we recommend to apply the rope thinner during periods of cold and clouded weather to maximize the thinning effect due to physiological stress and shortage of assimilates to the fruitlets. Concrete practical recommendations are given

The Role of Chemotherapy in Upper Tract Urothelial Carcinoma

Locally advanced upper tract urothelial carcinoma has a poor prognosis. While surgery represents the only potentially curable therapeutic intervention, recurrences are common and typically systemic in nature. It is thus reasonable to consider perioperative chemotherapy in an effort to decrease the risk of recurrence. There are very little direct data providing clinical guidance in this scenario. For urothelial cancer of the bladder, there are randomized phase III data demonstrating a survival advantage with neoadjuvant cisplatin-based combination chemotherapy. Although arguments favoring adjuvant chemotherapy could be made for upper tract urothelial cancer, the loss of renal function that occurs with nephrectomy can complicate administration of appropriate perioperative treatment. Therefore, by analogy to urothelial carcinoma of the lower tract, it is argued that cisplatin-based neoadjuvant chemotherapy should be the standard of care for patients with locally advanced upper tract urothelial cancer

Résumé des recommandations pratiques actuelles pour l’éclaircissage biologique des pommiers

Recommandations pratiques résultant des essais d’éclaircissage réalisés de 2003 à 2005 I), II)

Advantages of genomic complexity: bioinformatics opportunities in microRNA cancer signatures

MicroRNAs, small non-coding RNAs, may act as tumor suppressors or oncogenes, and each regulate their own transcription and that of hundreds of genes, often in a tissue-dependent manner. This creates a tightly interwoven network regulating and underlying oncogenesis and cancer biology. Although protein-coding gene signatures and single protein pathway markers have proliferated over the past decade, routine adoption of the former has been hampered by interpretability, reproducibility, and dimensionality, whereas the single molecule–phenotype reductionism of the latter is often overly simplistic to account for complex phenotypes. MicroRNA-derived biomarkers offer a powerful alternative; they have both the flexibility of gene expression signature classifiers and the desirable mechanistic transparency of single protein biomarkers. Furthermore, several advances have recently demonstrated the robust detection of microRNAs from various biofluids, thus providing an additional opportunity for obtaining bioinformatically derived biomarkers to accelerate the identification of individual patients for personalized therapy

Graphical prediction of quantum interference-induced transmission nodes in functionalized organic molecules

Quantum interference (QI) in molecular transport junctions can lead to dramatic reductions of the electron transmission at certain energies. In a recent work [Markussen et al., Nano Lett. 2010, 10, 4260] we showed how the presence of such transmission nodes near the Fermi energy can be predicted solely from the structure of a conjugated molecule when the energies of the atomic p_z orbitals do not vary too much. Here we relax the assumption of equal on-site energies and generalize the graphical scheme to molecules containing different atomic species. We use this diagrammatic scheme together with tight-binding and density functional theory calculations to investigate QI in linear molecular chains and aromatic molecules with different side groups. For the molecular chains we find a linear relation between the position of the transmission nodes and the side group pi orbital energy. In contrast, the transmission functions of functionalized aromatic molecules generally display a rather complex nodal structure due to the interplay between molecular topology and the energy of the side group orbital.Comment: 7 page

La Situació de la innovació a Catalunya

Els autors examinen l'estat i l'evolució de la capacitat innovadora del nostre país i del conjunt dels seus agents econòmics i socials, així com l'estat de les institucions que incideixen en aquesta capacitat innovadora, i identifiquen els possibles obstacles per tal de plantejar les polítiques que poden ser necessàries i efectives per millorar aquesta capacitat. A més, en determinen la influència en l'evolució de la productivitat del país i de les seves empreses. L'anàlisi quantitativa estudia la despesa en R+D, la despesa en innovació, els recursos humans dedicats a la innovació, l'evolució de les sol·licituds de patents i les exportacions catalanes segons la seva intensitat tecnològica.The authors examine the current state of and trends in the capacity to innovate of Catalonia and its economic and social agents, as well as the state of the institutions influencing this innovative capacity. They also identify the potential obstacles to propose those policies that may be necessary and effective to improve its innovative capacity. The authors also determine what influences the development of the productivity of Catalonia and its companies. This quantitative analysis studies R&D expenditure, expenditure on innovation, the human resources dedicated to innovation, trends in patent applications and Catalan exports according to technological intensity

La Situació de la innovació a Catalunya

Els autors examinen l'estat i l'evolució de la capacitat innovadora del nostre país i del conjunt dels seus agents econòmics i socials, així com l'estat de les institucions que incideixen en aquesta capacitat innovadora, i identifiquen els possibles obstacles per tal de plantejar les polítiques que poden ser necessàries i efectives per millorar aquesta capacitat. A més, en determinen la influència en l'evolució de la productivitat del país i de les seves empreses. L'anàlisi quantitativa estudia la despesa en R+D, la despesa en innovació, els recursos humans dedicats a la innovació, l'evolució de les sol·licituds de patents i les exportacions catalanes segons la seva intensitat tecnològica.The authors examine the current state of and trends in the capacity to innovate of Catalonia and its economic and social agents, as well as the state of the institutions influencing this innovative capacity. They also identify the potential obstacles to propose those policies that may be necessary and effective to improve its innovative capacity. The authors also determine what influences the development of the productivity of Catalonia and its companies. This quantitative analysis studies R&D expenditure, expenditure on innovation, the human resources dedicated to innovation, trends in patent applications and Catalan exports according to technological intensity

Temporal ordering of substitutions in RNA evolution : uncovering the structural evolution of the human accelerated region 1

The Human Accelerated Region 1 (HAR1) is the most rapidly evolving region in the human genome. It is part of two overlapping long non-coding RNAs, has a length of only 118 nucleotides and features 18 human specific changes compared to an ancestral sequence that is extremely well conserved across non-human primates. The human HAR1 forms a stable secondary structure that is strikingly different from the one in chimpanzee as well as other closely related species, again emphasizing its human-specific evolutionary history. This suggests that positive selection has acted to stabilize human-specific features in the ensemble of HAR1 secondary structures. To investigate the evolutionary history of the human HAR1 structure, we developed a computational model that evaluates the relative likelihood of evolutionary trajectories as a probabilistic version of a Hamiltonian path problem. The model predicts that the most likely last step in turning the ancestral primate HAR1 into the human HAR1 was exactly the substitution that distinguishes the modern human HAR1 sequence from that of Denisovan, an archaic human, providing independent support for our model. The MutationOrder software is available for download and can be applied to other instances of RNA structure evolution