Analysis of Plant Growth-Promoting Effects of Fluorescent Pseudomonas Strains Isolated from Mentha piperita Rhizosphere and Effects of Their Volatile Organic Compounds on Essential Oil Composition

Many species or strains of the genus Pseudomonas have been characterized as plant growth promoting rhizobacteria (PGPR). We used a combination of phenotypic and genotypic techniques to analyze the community of fluorescent Pseudomonas strains in the rhizosphere of commercially grown Mentha piperita (peppermint). Biochemical techniques, Amplified rDNA Restriction Analysis (ARDRA), and 16S rRNA gene sequence analysis revealed that the majority of the isolated native fluorescent strains were P. putida. Use of two Repetitive Sequence-based PCR (rep-PCR) techniques, BOX-PCR and ERIC-PCR, allowed us to evaluate diversity among the native strains and to more effectively distinguish among them. PGPR activity was tested for the native strains and reference strain P. fluorescens WCS417r. Micropropagated M. piperita plantlets were exposed to microbial volatile organic compounds (mVOCs) emitted by the bacterial strains, and plant biomass parameters and production of essential oils (EOs) were measured. mVOCs from 11 of the native strains caused an increase in shoot fresh weight. mVOCs from three native strains (SJ04, SJ25,SJ48) induced changes in M. pierita EO composition. The mVOCs caused a reduction of metabolites in the monoterpene pathway, for example menthofuran, and an increase in menthol production. Menthol production is the primary indicator of EO quality. The mVOCs produced by native strains SJ04, SJ25,SJ48 and strain WCS417r were analyzed. The obtained mVOC chromatographic profiles were unique for each of the three native strains analyzed, containing varying hydrocarbon, aromatic, and alogenic compounds. The differential effects of the strains were most likely due to the specific mixtures of mVOCs emitted by each strain, suggesting a synergistic effect occurs among the compounds present

Análisis Estructural de Tanque Cisterna Semirremolque Fabricado en Aleación de Aluminio para Transporte de Combustibles Líquidos

En este trabajo se estudia el comportamiento estructural de un tanque cisterna semirremolque de aleación de aluminio, fabricado por la firma Tanques Fangio S.A. de la ciudad de Pergamino, provincia de Buenos Aires. Mediante modelización y análisis a través del método de elementos finitos (FEM), se estudia el diseño ‘A’ (modelo clásico), para compararlo luego con un diseño ‘B’ (modelo americano). En ambos casos se estiman los esfuerzos estáticos internos a los cuales se encuentran sometidos los distintos componentes de la estructura del tanque semirremolque, respetando las cargas impuestas por las especificaciones DOT. La modelización, discretización tridimensional y cálculo FEM permiten abordar a los valores de tensiones y deformaciones presentes en ambos modelos. Se concluye que el modelo ‘B’ presenta mejor comportamiento estructural lográndose una mejor relación costo-beneficio

Estudio preliminar del comportamiento dinámico de un semirremolque cisterna ante perturbaciones impulsivas sobre sus ejes, en base a métodos numéricos

En un contexto de creciente necesidad de fortalecimiento de las relaciones entre los sectores productivo y académico para favorecer el desarrollo socio-económico del país, y con la finalidad de aportar soluciones relativas a la optimización de diseños mecánicos sobre la base de la utilización de herramientas computacionales, el presente trabajo se centra en el desarrollo general de un modelo representativo de un semirremolque cisterna para el transporte de combustibles líquidos, en términos de su respuesta dinámica ante perturbaciones de corta duración sobre sus ejes. Para ello, se recurre al software Matlab Simulink, idóneo para el desarrollo de un modelo numérico del comportamiento dinámico del objeto de estudio nombrado. A través del vínculo de cooperación mutua existente entre la empresa Tanques Fangio SA, de la ciudad de Pergamino, y la UTN-FRSN, se relevan y determinan los datos de entrada para dicho modelo, y se simula, a través de un análisis por elementos finitos (FEA), el campo global de tensiones y deformaciones resultantes de tales perturbaciones. Este estudio preliminar pretende sentar las bases de un modelo representativo del semirremolque cisterna, cuyo diseño fue provisto por la empresa Tanques Fangio SA, factible de aplicarse a vehículos similares y capaz de proveer datos significativos para posteriores análisis de diseños de componentes vehiculares. Se verificará la obtención de un modelo representativo del semirremolque cisterna, con información de análisis y diseño relevante. Si bien su rigurosa validación se tratará en un futuro trabajo de investigación, se detallará aquí un análisis de su representatividad, a través de datos de referencia pertinentes.In a context of growing need to strengthen relations between productive and academic sectors to improve the socioeconomic development of our country, and with the aim of providing solutions related to the optimization of mechanical designs, based on the use of potent computer software, this paper focuses on the general development of a representative model of a road tanker for the transport of liquid fuels, in terms of its dynamic response to short-term perturbation on its axes. For this, Matlab Simulink software is used, which is suitable for the development of a numerical model of the dynamic behaviour of the named object of study. Through the bond of mutual cooperation between the company Tanques Fangio SA, a tanker manufacturer located in Pergamino, Bs. As., and the UTN-FRSN, input data needed to perform the study is capable to be determined, and it is feasible to make a finite-element analysis (FEA) to obtain the global field of stresses and deformations of the structural part of the tanker, resulting from these disturbances. This preliminary study aims to lay the foundations of a representative model of the tanker, whose design was provided by the company Tanques Fangio SA, feasible to be applied to similar vehicles and capable of providing significant data for subsequent analysis of vehicle components designs. The obtaining of a representative model of the tanker will be verified, with relevant analysis and design information. Although its rigorous validation will be done in a future research work, an analysis of its representativeness will be performed here, through relevant reference data.Fil: Inzeo, Facundo Pascual. Universidad Tecnológica Nacional. Facultad Regional San Nicolás; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Modelado e Innovación Tecnológica. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Modelado e Innovación Tecnológica; ArgentinaFil: Sosa, Facundo. Universidad Tecnológica Nacional. Facultad Regional San Nicolás; ArgentinaFil: García, Bruno E.. Universidad Tecnológica Nacional. Facultad Regional San Nicolás; ArgentinaFil: Flores, Giuliano T.. Universidad Tecnológica Nacional. Facultad Regional San Nicolás; ArgentinaFil: Domínguez, Cristian P.. Universidad Tecnológica Nacional. Facultad Regional San Nicolás; ArgentinaFil: Franco, Neoren G.. Universidad Tecnológica Nacional. Facultad Regional San Nicolás; ArgentinaFil: Arrien, Patricio G.. No especifíca;Fil: Giordano, Walter F.. Universidad Tecnológica Nacional. Facultad Regional San Nicolás; Argentina2do Congreso sobre Medios de Transporte y sus Tecnologías AsociadasGeneral Pacheco, Buenos AiresArgentinaUniversidad Tecnológica Nacional. Facultad Regional General PachecoCentro de Investigación e Innovación Vehicula

Hacia una biblioteca virtual en la UNLu

Este proyecto se propone estudiar los problemas y posibles soluciones que conlleva la construcción de una Biblioteca Virtual. Abarca desde el diseño de la arquitectura del software, que permita administrar los recursos y protegerlos, hasta el estudio de los posibles modelos de negocios, que posibiliten la concesión de derechos de las editoriales (que poseen la propiedad intelectual de las obras) a éstas bibliotecas.Eje: Innovación en Sistemas de SoftwareRed de Universidades con Carreras en Informática (RedUNCI

Hacia una biblioteca virtual en la UNLu

Este proyecto se propone estudiar los problemas y posibles soluciones que conlleva la construcción de una Biblioteca Virtual. Abarca desde el diseño de la arquitectura del software, que permita administrar los recursos y protegerlos, hasta el estudio de los posibles modelos de negocios, que posibiliten la concesión de derechos de las editoriales (que poseen la propiedad intelectual de las obras) a éstas bibliotecas.Eje: Innovación en Sistemas de SoftwareRed de Universidades con Carreras en Informática (RedUNCI

Hacia una biblioteca virtual en la UNLu

Este proyecto se propone estudiar los problemas y posibles soluciones que conlleva la construcción de una Biblioteca Virtual. Abarca desde el diseño de la arquitectura del software, que permita administrar los recursos y protegerlos, hasta el estudio de los posibles modelos de negocios, que posibiliten la concesión de derechos de las editoriales (que poseen la propiedad intelectual de las obras) a éstas bibliotecas.Eje: Innovación en Sistemas de SoftwareRed de Universidades con Carreras en Informática (RedUNCI

Effectiveness of service screening: a case–control study to assess breast cancer mortality reduction

The aim of this study was the evaluation of the impact of service screening programmes on breast cancer mortality in five regions of Italy. We conducted a matched case–control study with four controls for each case. Cases were defined as breast cancer deaths occurred not later than 31 December 2002. Controls were sampled from the local municipality list and matched by date of birth. Screening histories were assessed by the local, computerised, screening database and subjects were classified as either invited or not-yet-invited and as either screened or unscreened. There were a total of 1750 breast cancer deaths within the 50 to 74-year-old breast cancer cases and a total of 7000 controls. The logistic conditional estimate of the cumulative odds ratios comparing invited with not-yet-invited women was 0.75 (95% CI: 0.62–0.92). Restricting the analyses to invited women, the odds ratio of screened to never-respondent women corrected for self-selection bias was 0.55 (95% CI: 0.36–0.85). The introduction of breast cancer screening programmes in Italy is associated with a reduction in breast cancer mortality attributable to the additional impact of service screening over and above the background access to mammography

Genomic, Pathway Network, and Immunologic Features Distinguishing Squamous Carcinomas

This integrated, multiplatform PanCancer Atlas study co-mapped and identified distinguishing molecular features of squamous cell carcinomas (SCCs) from five sites associated with smokin

Pan-Cancer Analysis of lncRNA Regulation Supports Their Targeting of Cancer Genes in Each Tumor Context

Long noncoding RNAs (lncRNAs) are commonly dys-regulated in tumors, but only a handful are known toplay pathophysiological roles in cancer. We inferredlncRNAs that dysregulate cancer pathways, onco-genes, and tumor suppressors (cancer genes) bymodeling their effects on the activity of transcriptionfactors, RNA-binding proteins, and microRNAs in5,185 TCGA tumors and 1,019 ENCODE assays.Our predictions included hundreds of candidateonco- and tumor-suppressor lncRNAs (cancerlncRNAs) whose somatic alterations account for thedysregulation of dozens of cancer genes and path-ways in each of 14 tumor contexts. To demonstrateproof of concept, we showed that perturbations tar-geting OIP5-AS1 (an inferred tumor suppressor) andTUG1 and WT1-AS (inferred onco-lncRNAs) dysre-gulated cancer genes and altered proliferation ofbreast and gynecologic cancer cells. Our analysis in-dicates that, although most lncRNAs are dysregu-lated in a tumor-specific manner, some, includingOIP5-AS1, TUG1, NEAT1, MEG3, and TSIX, synergis-tically dysregulate cancer pathways in multiple tumorcontexts

Pan-cancer Alterations of the MYC Oncogene and Its Proximal Network across the Cancer Genome Atlas

Although theMYConcogene has been implicated incancer, a systematic assessment of alterations ofMYC, related transcription factors, and co-regulatoryproteins, forming the proximal MYC network (PMN),across human cancers is lacking. Using computa-tional approaches, we define genomic and proteo-mic features associated with MYC and the PMNacross the 33 cancers of The Cancer Genome Atlas.Pan-cancer, 28% of all samples had at least one ofthe MYC paralogs amplified. In contrast, the MYCantagonists MGA and MNT were the most frequentlymutated or deleted members, proposing a roleas tumor suppressors.MYCalterations were mutu-ally exclusive withPIK3CA,PTEN,APC,orBRAFalterations, suggesting that MYC is a distinct onco-genic driver. Expression analysis revealed MYC-associated pathways in tumor subtypes, such asimmune response and growth factor signaling; chro-matin, translation, and DNA replication/repair wereconserved pan-cancer. This analysis reveals insightsinto MYC biology and is a reference for biomarkersand therapeutics for cancers with alterations ofMYC or the PMN