Ca2+ transients are not required as signals for long-term neurite outgrowth from cultured sympathetic neurons

A method for clamping cytosolic free Ca2+ ([Ca2+]i) in cultures of rat sympathetic neurons at or below resting levels for several days was devised to determine whether Ca2+ signals are required for neurite outgrowth from neurons that depend on Nerve Growth Factor (NGF) for their growth and survival. To control [Ca2+]i, normal Ca2+ influx was eliminated by titration of extracellular Ca2+ with EGTA and reinstated through voltage-sensitive Ca2+ channels. The rate of neurite outgrowth and the number of neurites thus became dependent on the extent of depolarization by KCl, and withdrawal of KCl caused an immediate cessation of growth. Neurite outgrowth was completely blocked by the L type Ca2+ channel antagonists nifedipine, nitrendipine, D600, or diltiazem at sub- or micromolar concentrations. Measurement of [Ca2+]i in cell bodies using the fluorescent Ca2+ indicator fura-2 established that optimal growth, similar to that seen in normal medium, was obtained when [Ca2+]i was clamped at resting levels. These levels of [Ca2+]i were set by serum, which elevated [Ca2+]i by integral of 30 nM, whereas the addition of NGF had no effect on [Ca2+]i. The reduction of [Ca2+]o prevented neurite fasciculation but this had no effect on the rate of neurite elongation or on the number of extending neurites. These results show that neurite outgrowth from NGF-dependent neurons occurs over long periods in the complete absence of Ca2+ signals, suggesting that Ca2+ signals are not necessary for operating the basic machinery of neurite outgrowth

Evidence for biquadratic exchange in the quasi-two-dimensional antiferromagnet FePS3_3

FePS$_3$ is a van der Waals compound with a honeycomb lattice that is a good example of a two-dimensional antiferromagnet with Ising-like anisotropy. Neutron spectroscopy data from FePS3 were previously analysed using a straight-forward Heisenberg Hamiltonian with a single-ion anisotropy. The analysis captured most of the elements of the data, however some significant discrepancies remained. The discrepancies were most obvious at the Brillouin zone boundaries. The data are subsequently reanalysed allowing for unequal exchange between nominally equivalent nearest-neighbours, which resolves the discrepancies. The source of the unequal exchange is attributed to a biquadratic exchange term in the Hamiltonian which most probably arises from a strong magnetolattice coupling. The new parameters show that there are features consistent with Dirac magnon nodal lines along certain Brillouin zone boundaries.Comment: 8 pages, 4 figures. The following article has been accepted by the Journal of Applied Physics. After it is published, it will be found at (https://publishing.aip.org/resources/librarians/products/journals/). The article was submitted as part of a special topic edition (https://publishing.aip.org/publications/journals/special-topics/jap/2d-quantum-materials-magnetism-and-superconductivity/

The Nitrogen, Phosphorus and Organic Carbon Content of Iowa Soils

During the past 20 years an investigation has been underway to determine the nitrogen, phosphorus and organic carbon content of the various soils of Iowa. Samples were taken of the various soil types mapped in each county and they were then analyzed. A statistical analysis of the results has been made to determine the average nitrogen, phosphorus and organic carbon content of each soil type, and also to determine the significance of the differences between types

The effects of lime on the Hydrogen-Ion concentration and base exchange complex of Grundy silt loam

In a study of the effects of various kinds and amounts of limestone on the chemical and bacteriological properties of certain southern Iowa soils, it was found by the authors (14) that the Grundy silt loam has a rather high absorptive capacity for lime and other basic materials. Although the lime requirement of this soil was approximately 3 tons of limestone per acre, the experiments indicated that considerably more than 3 tons of limestone were necessary to bring the pH to 7.0 or to completely saturate the exchange complex with bases. Quarry-run limestone applied in sufficient amounts to meet the lime requirement increased the pH from 5.19 to only 5.80 in 3 months\u27 time, whereas, three times that amount increased the pH to only 6.6. It was estimated by extrapolation that approximately 12.6 tons of limestone would be required to bring the reaction of this soil up to pH 7.0. Similarly, limestone sufficient to meet the lime requirement increased the degree of saturation of the base exchange complex with bases from 60.13 to 71.16 percent in 3 months, and three times that amount of limestone, or 9.87 tons per acre of the quarry-run material, increased the degree of saturation to only 82.9 percent

Effects of inoculation and liming on soybeans grown on the Grundy silt loam

Soybeans are grown extensively on the Grundy silt loam in southern Iowa. This soil usually requires from 2 to 3 tons of limestone to correct the acidity. Furthermore, it does not naturally contain the nitrogen-fixing soybean root-nodule bacteria. Hence, experiments were conducted to determine the effects of liming and inoculation on the yield and quality of soybeans grown on typical Grundy silt loam. The experiments were conducted near Osceola in Clarke County. Quarry-run limestone was applied to different plots in varying amounts, ranging from 1 to 6 tons per acre. On other plots limestone of different degrees of fineness was applied. Two plots were left untreated to serve as checks. At planting time inoculated soybeans were sown on half of each plot and seed without inoculation on the other half

Soil erosion in Iowa

In the late summer of 1934 the Soil Conservation Service began a reconnaissance erosion survey of the United States. The Soils Subsection of the Iowa Agricultural Experiment Station cooperated in Iowa, and the findings are reported in this bulletin.https://lib.dr.iastate.edu/specialreports/1000/thumbnail.jp

Preliminary submillimeter spectroscopic measurements using a submillimeter heterodyne radiometer

A submillimeter heterodyne radiometer uses a submillimeter laser, pumped by a CO2 laser, as a local oscillator and a room temperature Schottky barrier diode as the first IF mixer. The radiometer can resolve spectral lines in the submillimeter region of the spectrum (arising from pure rotational molecular transitions) to within 0.3 MHz, using acousto-optic spectrum analyzer which measures the power spectrum by simultaneously sampling 0.3 MHz wide channels over a 100 MHz bandwidth spanning the line. Preliminary observations of eight spectral lines of H2O2, CO, NH3 and H2O, all lying in the 434-524 micrometer wavelength range are described. All eight lines were observed using two local oscillator frequencies obtained by operating the submillimeter laser with either methyl fluoride (CH3F) or formic acid (HCOOH) as the lasing gas. Sample calculations of line parameters from the observed data show good agreement with established values. One development goal is the size and weight reduction of the package to make it suitable for balloon or shuttle experiments to detect trace gases in the upper atmosphere

The distance to the LMC cluster NGC 1866 and the surrounding field

We use the Main Sequence stars in the LMC cluster NGC 1866 and of Red Clump stars in the local field to obtain two independent estimates of the LMC distance. We apply an empirical Main Sequence-fitting technique based on a large sample of subdwarfs with accurate {\sl Hipparcos} parallaxes in order to estimate the cluster distance modulus, and the multicolor Red Clump method to derive distance and reddening of the LMC field. We find that the Main Sequence-fitting and the Red Clump distance moduli are in significant disagreement; NGC 1866 distance is equal to $\rm (m-M)_{0,NGC 1866}=18.33\pm$0.08 (consistent with a previous estimate using the same data and theoretical Main Sequence isochrones), while the field stars provide $\rm (m-M)_{0,field}=18.53\pm$0.07. This difference reflects the more general dichotomy in the LMC distance estimates found in the literature. Various possible causes for this disagreement are explored, with particular attention paid to the still uncertain metallicity of the cluster and the star formation history of the field stars.Comment: 5 pages, incl. 1 figure, uses emulateapj.sty, ApJ accepte

Higher Resolution VLBI Imaging with Fast Frequency Switching

Millimetre-VLBI is an important tool in AGN astrophysics, but it is limited by short atmospheric coherence times and poor receiver and antenna performance. We demonstrate a new kind of phase referencing for the VLBA, enabling us to increase the sensitivity in mm-VLBI by an order of magnitude. If a source is observed in short cycles between the target frequency, nu_t, and a reference frequency, nu_ref, the nu_t data can be calibrated using scaled-up phase solutions from self-calibration at nu_ref. We have demonstrated the phase transfer on 3C 279, where we were able to make an 86 GHz image with 90 % coherence compared to self-calibration at nu_t. We have detected M81, our science target in this project, at 86 GHz using the same technique. We describe scheduling strategy and data reduction. The main impacts of fast frequency switching are the ability to image some of the nearest, but relatively weak AGN cores with unprecedented high angular resolution and to phase-reference the nu_t data to the nu_ref core position, enabling the detection of possible core shifts in jets due to optical depth effects. This ability will yield important constraints on jet properties and might be able to discriminate between the two competing emission models of Blandford-Konigl jets and spherical advection-dominated accretion flows (ADAFs) in low-luminosity AGNs.Comment: 4 pages, 6 figures, appears in: Proceedings of the 6th European VLBI Network Symposium held on June 25th-28th in Bonn, Germany. Edited by: E. Ros, R.W. Porcas, A.P. Lobanov, and J.A. Zensu