\u3cem\u3eShort Communication\u3c/em\u3e: Effect of Storage Temperature on the Solubility of Milk Protein Concentrate 80 (MPC80) Treated with NaCL or KCL

A previous study in our laboratory showed that addition of 150 mM NaCl or KCl into diafiltration water improved the solubility of freshly made milk protein concentrate 80 (MPC80). In the present study, the objectives were (1) to evaluate the solubility of NaCl- or KCl-treated MPC80 samples kept at varying temperatures and then stored for extensive periods at room temperature (21°C ± 1°C); and (2) to determine if MPC80 samples stored at different temperatures and protein conformation can be grouped or categorized together. Freshly manufactured MPC80 samples were untreated (control), processed with NaCl, or processed with KCl. One set of sample bags was stored at 4°C; second and third sets of bags were kept at 25°C and 55°C for 1 mo (31 d) and then transferred to room temperature (21°C ± 1°C) storage conditions for 1 yr (365 d). Samples were tested for nitrogen solubility index (NSI) and for protein changes by Fourier-transform infrared (FTIR) spectroscopy. Analysis of variance results for NSI showed 2 significantly different groupings of MPC80 samples. The more soluble group contained samples treated with NaCl or KCl and stored at either 4°C or 25°C. These samples had mean NSI \u3e97.5%. The less soluble groups contained all control samples, regardless of storage temperature, and NaCl- or KCl-treated samples stored at 55°C. These samples had mean NSI from 39.5 to 58%. Within each of these groups (more soluble and less soluble), no significant differences in solubility were detected. Pattern recognition analysis by soft independent modeling of class analogy (SIMCA) was used to assess protein changes during storage by monitoring the amide I and amide II (1,700−1 to 1,300 cm−1) regions. Dominant bands were observed at 1,385 cm−1 for control, 1,551 cm−1 for KCl-treated samples, and 1,694 cm−1 for NaCl-treated samples. Moreover, SIMCA clustered the MPC80 samples stored at 4°C separately from samples stored at 25°C and 55°C. This study demonstrates that (1) the addition of NaCl or KCl during MPC80 manufacture reduces the deleterious changes in solubility upon prolonged storage at 4°C or 25°C, and (2) the solubility of samples stored at 55°C is poor irrespective of salt treatment

Diencephalic syndrome in child with NF-1 and hypothalamic tumour

We describe a 20 month old boy with neuro-fibromatosis type 1 (NF-1) who presented with diencephalic syndrome due to a large hypothalamic tumour and developed massive necrosis after chemotherapy associated with severe encephalopathy. We report this case because of rapid progression of presenting symptoms, the rare association with diencephalic syndrome in NF-1, chemotherapy induction of “tumour lysis” associated with encephalopathy, reduced toxicity and sustained improvement with vinblastine, the therapeutic benefit of tumour drainage signs of resolution of diencephalic syndrome and then restoration of visual movements and function associated with developmental recovery. The presentation of tumour in this case highlights the importance for parents and doctor to known and recognize the precocious symptoms, and justifies sharing these features as an indicator with parents and GP’s to justify early / urgent specialist review, particularly in the first two years of life. Early recognition could offer a reduced risk of brain injury

Slow, fast and in between: habitat distribution and behaviour of larvae in nine species of libellulid dragonfly

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72093/1/j.1365-2427.2007.01889.x.pd

A dedicated haem lyase is required for the maturation of a novel bacterial cytochrome c with unconventional covalent haem binding

In bacterial c-type cytochromes, the haem cofactor is covalently attached via two cysteine residues organized in a haem c-binding motif. Here, a novel octa-haem c protein, MccA, is described that contains only seven conventional haem c-binding motifs (CXXCH), in addition to several single cysteine residues and a conserved CH signature. Mass spectrometric analysis of purified MccA from Wolinella succinogenes suggests that two of the single cysteine residues are actually part of an unprecedented CX15CH sequence involved in haem c binding. Spectroscopic characterization of MccA identified an unusual high-potential haem c with a red-shifted absorption maximum, not unlike that of certain eukaryotic cytochromes c that exceptionally bind haem via only one thioether bridge. A haem lyase gene was found to be specifically required for the maturation of MccA in W. succinogenes. Equivalent haem lyase-encoding genes belonging to either the bacterial cytochrome c biogenesis system I or II are present in the vicinity of every known mccA gene suggesting a dedicated cytochrome c maturation pathway. The results necessitate reconsideration of computer-based prediction of putative haem c-binding motifs in bacterial proteomes

International variation in the definition of ‘main condition' in ICD-coded health data

Hospital-based medical records are abstracted to create International Classification of Disease (ICD) coded discharge health data in many countries. The ‘main condition' is not defined in a consistent manner internationally. Some countries employ a ‘reason for admission' rule as the basis for the main condition, while other countries employ a ‘resource use' rule. A few countries have recently transitioned from one of these approaches to the other. The definition of ‘main condition' in such ICD data matters when it is used to define a disease cohort to assign diagnosis-related groups and to perform risk adjustment. We propose a method of harmonizing the international definition to enable researchers and international organizations using ICD-coded health data to aggregate or compare hospital care and outcomes across countries in a consistent manner. Inter-observer reliability of alternative harmonization approaches should be evaluated before finalizing the definition and adopting it worldwid

Diencephalic syndrome in child with NF-1 and hypothalamic tumour

We describe a 20 month old boy with neuro-fibromatosis type 1 (NF-1) who presented with diencephalic syndrome due to a large hypothalamic tumour and developed massive necrosis after chemotherapy associated with severe encephalopathy.We report this case because of rapid progression of presenting symptoms, the rare association with diencephalic syndrome in NF-1, chemotherapy induction of “tumour lysis” associated with encephalopathy, reduced toxicity and sustained improvement with vinblastine, the therapeutic benefit of tumour drainage signs of resolution of diencephalic syndrome and then restoration of visual movements and function associated with developmental recovery. The presentation of tumour in this case highlights the importance for parents and doctor to known and recognize the precocious symptoms, and justifies sharing these features as an indicator with parents and GP’s to justify early / urgent specialist review, particularly in the first two years of life. Early recognition could offer a reduced risk of brain injury

Dietary trends inteh United States [1] (multiple letters)

The authors reply: To the Editor: Nusbaum and Eshleman suggest that dietary trends may reflect respondents’ adherence to the dietary guidelines of the time. From 1916 to 1976, nutritional recommendations relative to the basic food groups were fairly stable. The basic five food groups of 1916 were reduced to the basic four (two daily servings of milk and of meat, poultry, or eggs and four daily servings of fruits and vegetables and of breads and grains) in the 1960s. Not until the introduction of the U.S. Dietary Goals in 1977 did dietary guidelines explicitly recommend nutrient limitations for fats, sugars, and cholesterol. Neither our group nor others have attempted to quantify the dietary guidelines of the 1960s or to ascertain who followed the guidelines most closely

Public Health Liberation – An Emerging Transdiscipline to Elucidate and Transform the Public Health Economy

Public Health Liberation (PHL) is an innovative general theory of public health aimed at accelerating health equity. This paper provides a rich synthesis of philosophical traditions, novel theories, and approaches to establish the basis for a new public health transdiscipline. The authors argue that the public health economy as a single analytic lens elucidates the contradictions and tensions that reproduce vast health inequity. Authored by a majority of Black women, community experiences and perspectives are a major strength of this paper because they draw upon leadership experiences with contemporary issues. The authors begin by describing their background in public health advocacy and by demonstrating the need for PHL using lead-contaminated water crises from Flint, Michigan and Washington, DC. They discuss the benefits of horizontal and vertical integration that broaden public health discourse to include affected populations and that seek opportunities throughout the public health economy. Their philosophical and theoretical reasoning reinterprets and adopts disciplinary concepts in political theory, sociology, women\u27s studies, African American emancipatory writing, anti-racism, and community psychology to form a culturally relevant worldview and cogent thesis. Several new constructs emerge that do not appear elsewhere in the literature - Gaze of the Enslaved, Morality Principle, liberation, illiberation, liberation safe spaces, public health realism, and hegemony. The authors use their ethical and theoretical assumptions to guide practice and community self-help. Public Health Liberation presents a major challenge to assumptions about public health effectiveness in addressing vast health inequity