DNA double strand breaks but not interstrand crosslinks prevent progress through meiosis in fully grown mouse oocytes

There is some interest in how mammalian oocytes respond to different types of DNA damage because of the increasing expectation of fertility preservation in women undergoing chemotherapy. Double strand breaks (DSBs) induced by ionizing radiation and agents such as neocarzinostatin (NCS), and interstrand crosslinks (ICLs) induced by alkylating agents such as mitomycin C (MMC), are toxic DNA lesions that need to be repaired for cell survival. Here we examined the effects of NCS and MMC treatment on oocytes collected from antral follicles in mice, because potentially such oocytes are readily collected from ovaries and do not need to be in vitro grown to achieve meiotic competency. We found that oocytes were sensitive to NCS, such that this ionizing radiation mimetic blocked meiosis I and caused fragmented DNA. In contrast, MMC had no impact on the completion of either meiosis I or II, even at extremely high doses. However, oocytes treated with MMC did show ?-H2AX foci and following their in vitro maturation and parthenogenetic activation the development of the subsequent embryos was severely compromised. Addition of MMC to 1-cell embryos caused a similarly poor level of development, demonstrating oocytes have eventual sensitivity to this ICL-inducing agent but this does not occur during their meiotic division. In oocytes, the association of Fanconi Anemia protein, FANCD2, with sites of ICL lesions was not apparent until entry into the embryonic cell cycle. In conclusion, meiotic maturation of oocytes is sensitive to DSBs but not ICLs. The ability of oocytes to tolerate severe ICL damage and yet complete meiosis, means that this type of DNA lesion goes unrepaired in oocytes but impacts on subsequent embryo quality

A New Class of Solutions to the Strong CP Problem with a Small Two-Loop theta

We present a new class of models which produce zero theta (QCD} angle at the tree and one-loop level due to hermiticity of sub-blocks in the extended quark mass matrices. The structure can be maintained typically by non-abelian generation symmetry. Two examples are given for this class of solutions.Comment: 4 pages, 2 figure

Corporate Brand Image: Antecedents, Mediating Role and Impact on Stakeholder Expectations

Corporate identity and image are shaped by the entirety of perceptions of a variety of stakeholders, both existing and potential ones, such as customers, suppliers, employees, general public, opinion makers, and government officials. Our study is an empirical investigation of corporate brand image and impact on one such stakeholder group made up of prospective employees. A large multinational, a technical and scientific research firm, a major recruiter of graduates from campuses of reputable universities across the nation was chosen as the subject. This paper develops and tests a path model of the antecedent factors affecting corporate brand image and specific expectations that stakeholders may have of a corporate brand. Based on a thorough literature review, a corporate brand image model that treated as antecedents the four constructs, awareness of organisation’s products, perceptions about culture, personality of the focal firm, and general expectations was proposed. Specific expectations that respondents may have about working with the firm was modeled to be influenced by the corporate brand image of the firm surveyed and also by the mentioned four antecedent constructs. The model is fitted to empirical data obtained from a national sample of 368 respondents using LISREL 8.5 methodology, and strong support was found for five, partial support for two of the nine hypotheses tested. Substantial evidence can be seen for the mediating role of corporate brand image in shaping specific expectations that stakeholders have from the corporate brand. Practical significance and managerial implications for marketing investments and organisational performance are detailed.

Enhancing the Critical Current of a Superconducting Film in a Wide Range of Magnetic Fields with a Conformal Array of Nanoscale Holes

The maximum current (critical current) a type-II superconductor can transmit without energy loss is limited by the motion of the quantized magnetic flux penetrating into a superconductor. Introducing nanoscale holes into a superconducting film has been long pursued as a promising way to increase the critical current. So far the critical current enhancement was found to be mostly limited to low magnetic fields. Here we experimentally investigate the critical currents of superconducting films with a conformal array of nanoscale holes that have non-uniform density while preserving the local ordering. We find that the conformal array of nanoscle holes provides a more significant critical current enhancement at high magnetic fields. The better performance can be attributed to its arching effect that not only gives rise to the gradient in hole-density for pinning vortices with a wide range of densities but also prevent vortex channeling occurring in samples with a regular lattice of holes.Comment: 5 pages, 3 figure

Topological Phases in Neuberger-Dirac operator

The response of the Neuberger-Dirac fermion operator D=\Id + V in the topologically nontrivial background gauge field depends on the negative mass parameter $m_0$ in the Wilson-Dirac fermion operator $D_w$ which enters $D$ through the unitary operator $V = D_w (D_w^{\dagger} D_w)^{-1/2}$. We classify the topological phases of $D$ by comparing its index to the topological charge of the smooth background gauge field. An exact discrete symmetry in the topological phase diagram is proved for any gauge configurations. A formula for the index of D in each topological phase is derived by obtaining the total chiral charge of the zero modes in the exact solution of the free fermion propagator.Comment: 27 pages, Latex, 3 figures, appendix A has been revise

Antitumor activity of the tea polyphenol epigallocatechin-3-gallate encapsulated in targeted vesicles after intravenous administration

The therapeutic potential of epigallocatechin gallate, a green tea polyphenol with anti-cancer properties, is limited by its inability to specifically reach tumors following intravenous administration. The purpose of this study is to determine whether a tumor-targeted vesicular formulation of epigallocatechin gallate would suppress the growth of A431 epidermoid carcinoma and B16-F10 melanoma in vitro and in vivo. Transferrin-bearing vesicles encapsulating epigallocatechin gallate were intravenously administered to mice bearing subcutaneous A431 and B16-F10 tumors. The intravenous administration of epigallocatechin gallate encapsulated in transferrin-bearing vesicles resulted in tumor suppression for 40% of A431 and B16-F10 tumors. Animal survival was improved by more than 20 days compared to controls. Encapsulation of epigallocatechin gallate in transferrin-bearing vesicles is a promising therapeutic strategy

Suppression and Enhancement of Soliton Switching During Interaction in Periodically Twisted Birefringent Fiber

Soliton interaction in periodically twisted birefringent optical fibers has been analysed analytically with refernce to soliton switching. For this purpose we construct the exact general two-soliton solution of the associated coupled system and investigate its asymptotic behaviour. Using the results of our analytical approach we point out that the interaction can be used as a switch to suppress or to enhance soliton switching dynamics, if one injects multi-soliton as an input pulse in the periodically twisted birefringent fiber.Comment: 10 pages, 4 figures, Latex, submitted to Phys. Rev.