FGF2 is expressed in human and murine embryonic choroid plexus and affects choroid plexus cell behaviour

<p>Abstract</p> <p>Background</p> <p>Although fibroblast growth factor (Fgf) signalling plays crucial roles in several developing and mature tissues, little information is currently available on expression of Fgf2 during early choroid plexus development and whether Fgf2 directly affects the behaviour of the choroid plexus epithelium (CPe). The purpose of this study was to investigate expression of Fgf2 in rodent and human developing CPe and possible function of Fgf2, using <it>in vitro </it>models. The application of Fgf2 to brain <it>in vivo </it>can affect the whole tissue, making it difficult to assess specific responses of the CPe.</p> <p>Methods</p> <p>Expression of Fgf2 was studied by immunohistochemistry in rodent and human embryonic choroid plexus. Effects of Fgf2 on growth, secretion, aggregation and gene expression was investigated using rodent CPe vesicles, a three-dimensional polarized culture model that closely mimics CPe properties <it>in vivo</it>, and rodent CPe monolayer cultures.</p> <p>Results</p> <p>Fgf2 was present early in development of the choroid plexus both in mouse and human, suggesting the importance of this ligand in Fgf signalling in the developing choroid plexus. Parallel analysis of Fgf2 expression and cell proliferation during CP development suggests that Fgf2 is not involved in CPe proliferation <it>in vivo</it>. Consistent with this observation is the failure of Fgf2 to increase proliferation in the tri-dimensional vesicle culture model. The CPe however, can respond to Fgf2 treatment, as the diameter of CPe vesicles is significantly increased by treatment with this growth factor. We show that this is due to an increase in cell aggregation during vesicle formation rather than increased secretion into the vesicle lumen. Finally, Fgf2 regulates expression of the CPe-associated transcription factors, <it>Foxj1 </it>and <it>E2f5</it>, whereas transthyretin, a marker of secretory activity, is not affected by Fgf2 treatment.</p> <p>Conclusion</p> <p>Fgf2 expression early in the development of both human and rodent choroid plexus, and its ability to modulate behaviour and gene expression in CPe, supports the view that Fgf signalling plays a role in the maintenance of integrity and function of this specialized epithelium, and that this role is conserved between rodents and humans.</p

Effort and catch estimates for northern and central California marine recreational fisheries, 1981-1986

Nearly 200 species of finfish are taken by the marine recreational fishery along the northern and central California coast. This data report provides estimates of total effort, total catch, and fishery demographics for the years 1981 through 1986 for that fishery. Catch estimate data are presented by number and weight of species, by disposition of the fish caught (e.g. kept or thrown back), by type of access and fishing gear used, and by geographic zone. (311pp.

Regularity estimates up to the boundary for elliptic systems of difference equations

Regularity estimates up to the boundary for solutions of elliptic systems of finite difference equations were proved. The regularity estimates, obtained for boundary fitted coordinate systems on domains with smooth boundary, involve discrete Sobolev norms and are proved using pseudo-difference operators to treat systems with variable coefficients. The elliptic systems of difference equations and the boundary conditions which are considered are very general in form. The regularity of a regular elliptic system of difference equations was proved equivalent to the nonexistence of eigensolutions. The regularity estimates obtained are analogous to those in the theory of elliptic systems of partial differential equations, and to the results of Gustafsson, Kreiss, and Sundstrom (1972) and others for hyperbolic difference equations

Investigating the origin of cyclical wind variability in hot, massive stars - II. Hydrodynamical simulations of co-rotating interaction regions using realistic spot parameters for the O giant ξ\xi Persei

OB stars exhibit various types of spectral variability historically associated with wind structures, including the apparently ubiquitous discrete absorption components (DACs). These features have been proposed to be caused either by magnetic fields or non-radial pulsations. In this second paper of this series, we revisit the canonical phenomenological hydrodynamical modelling used to explain the formation of DACs by taking into account modern observations and more realistic theoretical predictions. Using constraints on putative bright spots located on the surface of the O giant $\xi$ Persei derived from high precision space-based broadband optical photometry obtained with the Microvariability and Oscillations of STars (MOST) space telescope, we generate two-dimensional hydrodynamical simulations of co-rotating interaction regions in its wind. We then compute synthetic ultraviolet (UV) resonance line profiles using Sobolev Exact Integration and compare them with historical timeseries obtained by the International Ultraviolet Explorer (IUE) to evaluate if the observed behaviour of $\xi$ Persei's DACs is reproduced. Testing three different models of spot size and strength, we find that the classical pattern of variability can be successfully reproduced for two of them: the model with the smallest spots yields absorption features that are incompatible with observations. Furthermore, we test the effect of the radial dependence of ionization levels on line driving, but cannot conclusively assess the importance of this factor. In conclusion, this study self-consistently links optical photometry and UV spectroscopy, paving the way to a better understanding of cyclical wind variability in massive stars in the context of the bright spot paradigm.Comment: 16 pages, 10 figures, accepted for publication by MNRA

Orbital parameters, chemical composition, and magnetic field of the Ap binary HD 98088

HD 98088 is a synchronised, double-lined spectroscopic binary system with a magnetic Ap primary component and an Am secondary component. We study this rare system using high-resolution MuSiCoS spectropolarimetric data, to gain insight into the effect of binarity on the origin of stellar magnetism and the formation of chemical peculiarities in A-type stars. Using a new collection of 29 high-resolution Stokes VQU spectra we re-derive the orbital and stellar physical parameters and conduct the first disentangling of spectroscopic observations of the system to conduct spectral analysis of the individual stellar components. From this analysis we determine the projected rotational velocities of the stars and conduct a detailed chemical abundance analysis of each component using both the SYNTH3 and ZEEMAN spectrum synthesis codes. The surface abundances of the primary component are typical of a cool Ap star, while those of the secondary component are typical of an Am star. We present the first magnetic analysis of both components using modern data. Using Least-Squares Deconvolution, we extract the longitudinal magnetic field strength of the primary component, which is observed to vary between +1170 and -920 G with a period consistent with the orbital period. There is no field detected in the secondary component. The magnetic field in the primary is predominantly dipolar, with the positive pole oriented approximately towards the secondary.Comment: Accepted for publication by MNRAS, 17 pages, 12 figure