Brettanomyces bruxellensis yeasts: impact on wine and winemaking

Yeasts belonging to the Brettanomyces/Dekkera genus are non-conventional yeasts, which affect winemaking by causing wine spoilage all over the world. This mini-review focuses on recent results concerning the presence of Brettanomyces bruxellensis throughout the wine processing chain. Here, culture-dependent and independent methods to detect this yeast on grapes and at the very early stage of wine production are encompassed. Chemical, physical and biological tools, devised for the prevention and control of such a detrimental species during winemaking are also presented. Finally, the mini-review identifies future research areas relevant to the improvement of wine safety and sensory profiles

P-31 NUCLEAR MAGNETIC-RESONANCE STUDIES OF INTRACELLULAR PH, PHOSPHATE COMPARTMENTATION AND PHOSPHATE-TRANSPORT IN YEASTS

31p NMR spectra were obtained from suspensions of Candida utilis, Saccharomyces cerevisiae and Zygosaccharomyces bailii grown aerobically on glucose. Direct introduction of substrate into the cell suspension, without interruption of the measurements, revealed rapid changes in pH upon addition of the energy source. All 3 ~p NMR spectra of the yeasts studied indicated the presence of two major intracellular inorganic phosphate pools at different pH environments. The pool at the higher pH was assigned to cytoplasmic phosphate from its response to glucose addition and iodoacetate inhibition of glycolysis. After addition of substrate the pH in the compartment containing the second phosphate pool decreased. A parallel response was observed for a significant fraction of the terminal and penultimate phosphates of the polyphosphate observed by 31 PNMR. This suggested that the inorganic phosphate fraction at the lower pH and the polyphosphates originated from the same intracellular compartment, most probably the vacuole. In this vacuolar compartment, pH is sensitive to metabolic conditions. In the presence of energy source a pH gradient as large as 0.8 to 1.5 units could be generated across the vacuolar membrane. Under certain conditions net transport of inorganic phosphate across the vacuolar membrane was observed during glycolysis: to the cytoplasm when the cytoplasmic phosphate concentration had become very low due to sugar phosphorylation, and into the vacuole when the former concentration had become high again after glucose exhaustion.Applied Science

Chenier-type ridges in Giralia Bay (Exmouth Gulf, Western Australia) - processes, chronostratigraphy, and significance for recording past tropical cyclones

Past coastal flooding events may be inferred from geomorphic and sedimentary archives, including particular landforms (e.g., beach ridges, washover fans), deposits (e.g., washover sediments in lagoons) or erosional features (e.g., erosional scarps within strandplains). In Giralia Bay, southern Exmouth Gulf (Western Australia), sandy ridge sequences in supratidal elevations form the landward margin of extensive mudflats. The formation of these ridges is assumed to be mainly driven by tropical cyclones (TCs), although their depositional processes need to be clarified. We investigated the supratidal sandy ridge sequence in Giralia Bay by carrying out process monitoring, geomorphological mapping by means of an unmanned aerial vehicle survey, as well as sedimentological and geochronological investigations and multivariate statistics. Based on the resulting data, this study aims at (i) identifying the most important driving processes to form the sandy ridges; (ii) establishing their chronostratigraphy; and (iii) understanding their significance for recording past TC activity. Trench excavations revealed sandy units that are interbedded with mud layers at the base, similar to the present distal mudflat sediments. On top, mud intercalations recede, and sand layers of varying grain size distribution dominate. In the upper part of the trenches, younger sediment layers onlap older ones documenting the stepwise seaward accretion of the ridges onto the mudflat. While our data suggests that tidal processes have only limited effects on ridge activity, sediment transport, erosion and deposition seems to be driven by both TC-induced storm surges and high magnitude precipitation events causing surface discharge. Most accretionary sand units are thus assumed to represent events of morphodynamic activity during TC-induced flooding since the mid-Holocene. Ridge activity is recorded in a roughly decadal resolution and over historical as well as prehistorical/Holocene time scales. While the ridges do not represent beach or chenier ridges sensu stricto, they may be described as chenier-type ridges due to their stratigraphical architecture. Ridge evolution, however, over a millennial time scale seems to be indicated by the landward rise of the sequence possibly corresponding to the mid-Holocene sea-level highstand of Western Australia of at least 1–2 m above present mean sea level