Augmented Atherogenesis in LDL Receptor Deficient Mice Lacking Both Macrophage ABCA1 and ApoE

ABCA1 protects against atherosclerosis by facilitating cholesterol efflux from macrophage foam cells in the arterial wall to extracellular apolipoprotein (apo) A-I. In contrast to apoA-I, apoE is secreted by macrophages and can, like apoA-I, induce ABCA1-mediated cholesterol efflux. Yet, the combined effect of macrophage ABCA1 and apoE on lesion development is unexplored.LDL receptor knockout (KO) mice were transplanted with bone marrow from ABCA1/apoE double KO (dKO) mice, their respective single KO's, and wild-type (WT) controls and were challenged with a high-fat/high-cholesterol diet for 9 weeks. In vitro cholesterol efflux experiments showed no differences between ABCA1 KO and dKO macrophages. The serum non-HDL/HDL ratio in dKO transplanted mice was 1.7-fold and 2.4-fold (p<0.01) increased compared to WT and ABCA1 KO transplanted mice, respectively. The atherosclerotic lesion area in dKO transplanted animals (650±94×10(3) µm(2)), however, was 1.9-fold (p<0.01) and 1.6-fold (p<0.01) increased compared to single knockouts (ABCA1 KO: 341±20×10(3) µm(2); apoE KO: 402±78×10(3) µm(2), respectively) and 3.1-fold increased (p<0.001) compared to WT (211±20×10(3) µm(2)). When normalized for serum cholesterol exposure, macrophage ABCA1 and apoE independently protected against atherosclerotic lesion development (p<0.001). Moreover, hepatic expression levels of TNFα and IL-6 were highly induced in dKO transplanted animals (3.0-fold; p<0.05, and 4.3-fold; p<0.001, respectively). In agreement, serum IL-6 levels were also enhanced in ABCA1 KO transplanted mice (p<0.05) and even further enhanced in dKO transplanted animals (3.1-fold as compared to ABCA1 KO transplanted animals; p<0.05).Combined deletion of macrophage ABCA1 and apoE results in a defect in cholesterol efflux and, compared to ABCA1 KO transplanted mice, elevated serum total cholesterol levels. Importantly, these mice also suffer from enhanced systemic and hepatic inflammation, together resulting in the observed augmented atherosclerotic lesion development

Dental calculus evidence of Taï Forest Chimpanzee plant consumption and life history transitions

Dental calculus (calcified dental plaque) is a source of multiple types of data on life history. Recent research has targeted the plant microremains preserved in this mineralised deposit as a source of dietary and health information for recent and past populations. However, it is unclear to what extent we can interpret behaviour from microremains. Few studies to date have directly compared the microremain record from dental calculus to dietary records, and none with long-term observation dietary records, thus limiting how we can interpret diet, food acquisition and behaviour. Here we present a high-resolution analysis of calculus microremains from wild chimpanzees (Pan troglodytes verus) of Taï National Park, Côte d"Ivoire. We test microremain assemblages against more tan two decades of field behavioural observations to establish the ability of calculus to capture the composition of diet. Our results show that some microremain classes accumulate as long-lived dietary markers. Phytolith abundance in calculus can reflect the proportions of plants in the diet, yet this pattern is not true for starches. We also report microremains can record information about other dietary behaviours, such as the age of weaning and learned food processing techniques like nutcracking

Plant exploitation in Neolithic Sudan: A review in the light of new data from the cemeteries R12 and Ghaba

Little is known about the introduction of domesticated crops in Sudan. Substantial early evidence of the cereals wheat and barley has, until recently, been mainly restricted to the post-Neolithic, third millennium BC pre-Kerma site on Sai Island, and prehistoric finds in general are scarce. Interestingly, an analysis of phytoliths from plant depositions within burials and phytoliths and starch from dental calculus from the Nubian Middle Neolithic cemetery R12 and the Early Neolithic cemetery of Ghaba in Central Sudan has recently set back the date of domesticated cereal introduction in Sudan and Egypt by 500 years to around 7000 years ago. This paper presents new plant identifications from R12 and Ghaba that confirm the earlier data and give new information on the use of plants in burial contexts, including indications of processing of panicoid grasses at Ghaba. In addition, the paper presents an overview of the archaeobotanical data from Mesolithic and Neolithic Sudan and provides information about grass exploitation of mid-Holocene Egyptian sites that enables further interpretation of the R12 and Ghaba data. The grave goods from R12 and Ghaba, supported by comparable finds from other sites, show that the commonly attested mid-Holocene cemeteries offer a valuable archive that can substantially improve the understanding of the importance of both wild and domesticated plants in Sudan at the time of Neolithisation. In addition, the unexpected early presence of the domesticated cereals wheat and barley in Nubia, supported by finds from later periods, raises the hypothesis that cereal cultivation was practiced in this region from at least the Middle Neolithic onwards

Plant exploitation in Neolithic Sudan: A review in the light of new data from the cemeteries R12 and Ghaba

Little is known about the introduction of domesticated crops in Sudan. Substantial early evidence of the cereals wheat and barley has, until recently, been mainly restricted to the post-Neolithic, third millennium BC pre-Kerma site on Sai Island, and prehistoric finds in general are scarce. Interestingly, an analysis of phytoliths from plant depositions within burials and phytoliths and starch from dental calculus from the Nubian Middle Neolithic cemetery R12 and the Early Neolithic cemetery of Ghaba in Central Sudan has recently set back the date of domesticated cereal introduction in Sudan and Egypt by 500 years to around 7000 years ago. This paper presents new plant identifications from R12 and Ghaba that confirm the earlier data and give new information on the use of plants in burial contexts, including indications of processing of panicoid grasses at Ghaba. In addition, the paper presents an overview of the archaeobotanical data from Mesolithic and Neolithic Sudan and provides information about grass exploitation of mid-Holocene Egyptian sites that enables further interpretation of the R12 and Ghaba data. The grave goods from R12 and Ghaba, supported by comparable finds from other sites, show that the commonly attested mid-Holocene cemeteries offer a valuable archive that can substantially improve the understanding of the importance of both wild and domesticated plants in Sudan at the time of Neolithisation. In addition, the unexpected early presence of the domesticated cereals wheat and barley in Nubia, supported by finds from later periods, raises the hypothesis that cereal cultivation was practiced in this region from at least the Middle Neolithic onwards