New live screening of plant-nematode interactions in the rhizosphere

Abstract Free living nematodes (FLN) are microscopic worms found in all soils. While many FLN species are beneficial to crops, some species cause significant damage by feeding on roots and vectoring viruses. With the planned legislative removal of traditionally used chemical treatments, identification of new ways to manage FLN populations has become a high priority. For this, more powerful screening systems are required to rapidly assess threats to crops and identify treatments efficiently. Here, we have developed new live assays for testing nematode responses to treatment by combining transparent soil microcosms, a new light sheet imaging technique termed Biospeckle Selective Plane Illumination Microscopy (BSPIM) for fast nematode detection, and Confocal Laser Scanning Microscopy for high resolution imaging. We show that BSPIM increased signal to noise ratios by up to 60 fold and allowed the automatic detection of FLN in transparent soil samples of 1.5 mL. Growing plant root systems were rapidly scanned for nematode abundance and activity, and FLN feeding behaviour and responses to chemical compounds observed in soil-like conditions. This approach could be used for direct monitoring of FLN activity either to develop new compounds that target economically damaging herbivorous nematodes or ensuring that beneficial species are not negatively impacted

ICAR: endoscopic skull‐base surgery

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Chenier-type ridges in Giralia Bay (Exmouth Gulf, Western Australia) - processes, chronostratigraphy, and significance for recording past tropical cyclones

Past coastal flooding events may be inferred from geomorphic and sedimentary archives, including particular landforms (e.g., beach ridges, washover fans), deposits (e.g., washover sediments in lagoons) or erosional features (e.g., erosional scarps within strandplains). In Giralia Bay, southern Exmouth Gulf (Western Australia), sandy ridge sequences in supratidal elevations form the landward margin of extensive mudflats. The formation of these ridges is assumed to be mainly driven by tropical cyclones (TCs), although their depositional processes need to be clarified. We investigated the supratidal sandy ridge sequence in Giralia Bay by carrying out process monitoring, geomorphological mapping by means of an unmanned aerial vehicle survey, as well as sedimentological and geochronological investigations and multivariate statistics. Based on the resulting data, this study aims at (i) identifying the most important driving processes to form the sandy ridges; (ii) establishing their chronostratigraphy; and (iii) understanding their significance for recording past TC activity. Trench excavations revealed sandy units that are interbedded with mud layers at the base, similar to the present distal mudflat sediments. On top, mud intercalations recede, and sand layers of varying grain size distribution dominate. In the upper part of the trenches, younger sediment layers onlap older ones documenting the stepwise seaward accretion of the ridges onto the mudflat. While our data suggests that tidal processes have only limited effects on ridge activity, sediment transport, erosion and deposition seems to be driven by both TC-induced storm surges and high magnitude precipitation events causing surface discharge. Most accretionary sand units are thus assumed to represent events of morphodynamic activity during TC-induced flooding since the mid-Holocene. Ridge activity is recorded in a roughly decadal resolution and over historical as well as prehistorical/Holocene time scales. While the ridges do not represent beach or chenier ridges sensu stricto, they may be described as chenier-type ridges due to their stratigraphical architecture. Ridge evolution, however, over a millennial time scale seems to be indicated by the landward rise of the sequence possibly corresponding to the mid-Holocene sea-level highstand of Western Australia of at least 1–2 m above present mean sea level