1,146 research outputs found

    Antibody validation of immunohistochemistry for biomarker discovery: Recommendations of a consortium of academic and pharmaceutical based histopathology researchers

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    As biomarker discovery takes centre-stage, the role of immunohistochemistry within that process is increasing. At the same time, the number of antibodies being produced for ‘‘research use’’ continues to rise and it is important that antibodies to be used as biomarkers are validated for specificity and sensitivity before use. This guideline seeks to provide a stepwise approach for the validation of an antibody for immunohistochemical assays, reflecting the views of a consortium of academic and pharmaceutical based histopathology researchers. We propose that antibodies are placed into a tier system, level 1–3, based on evidence of their usage in immunohistochemistry, and that the degree of validation required is proportionate to their place on that tier

    Applications of a new fluorimetric enzyme assay for the diagnosis of aspartylglucosaminuria

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    L-Aspartic acid-β-7-amido-4-methylcoumarin is a sensitive and specific fluorogenic substrate for lysosomal glycoasparaginase (aspartylgluco-saminidase). Fibroblasts and leukocytes from 8 patients with aspartylglucosaminuria, showed 1-7% of the mean normal glycoasparaginase activity. Heterozygotes showed intermediate activities. Glycoasparaginase activity in chorionic villi, cultured trophoblasts, cultured amniotic fluid cells and amniotic fluid was readily detectable, indicating that prenatal analysis of aspartylglucosaminuria should be possible with this assay. β-Aspartyl-4-methylumbelliferone was synthesized but this potential substrate can not be used to assay glycoasparaginase since it hydrolyses spontaneously

    Neuropsychological constraints to human data production on a global scale

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    Which are the factors underlying human information production on a global level? In order to gain an insight into this question we study a corpus of 252-633 Million publicly available data files on the Internet corresponding to an overall storage volume of 284-675 Terabytes. Analyzing the file size distribution for several distinct data types we find indications that the neuropsychological capacity of the human brain to process and record information may constitute the dominant limiting factor for the overall growth of globally stored information, with real-world economic constraints having only a negligible influence. This supposition draws support from the observation that the files size distributions follow a power law for data without a time component, like images, and a log-normal distribution for multimedia files, for which time is a defining qualia.Comment: to be published in: European Physical Journal

    Novel Approach to Confront Electroweak Data and Theory

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    A novel approach to study electroweak physics at one-loop level in generic SU(2)L×U(1)Y{\rm SU(2)_L \times U(1)_Y} theories is introduced. It separates the 1-loop corrections into two pieces: process specific ones from vertex and box contributions, and universal ones from contributions to the gauge boson propagators. The latter are parametrized in terms of four effective form factors eˉ2(q2)\bar{e}^2(q^2), sˉ2(q2)\bar{s}^2(q^2), gˉZ2(q2)\bar{g}_Z^2(q^2) and gˉW2(q2)\bar{g}_W^2 (q^2) corresponding to the γγ\gamma\gamma, γZ\gamma Z, ZZZZ and WWWW propagators. Under the assumption that only the Standard Model contributes to the process specific corrections, the magnitudes of the four form factors are determined at q2=0q^2=0 and at q^2=\mmz by fitting to all available precision experiments. These values are then compared systematically with predictions of SU(2)L×U(1)Y{\rm SU(2)_L \times U(1)_Y} theories. In all fits \alpha_s(\mz) and \bar{\alpha}(\mmz) are treated as external parameters in order to keep the interpretation as flexible as possible. The treatment of the electroweak data is presented in detail together with the relevant theoretical formulae used to interpret the data. No deviation from the Standard Model has been identified. Ranges of the top quark and Higgs boson masses are derived as functions of \alpha_s(\mz) and \bar{\alpha}(\mmz). Also discussed are consequences of the recent precision measurement of the left-right asymmetry at SLC as well as the impact of a top quark mass and an improved WW mass measurement.Comment: 123 pages, LaTeX (33 figures available via anonymous ftp), KEK-TH-375, KEK preprint 93-159, KANAZAWA-94-19, DESY 94-002, YUMS 94-22, SNUTP 94-82, to be published in Z.Phys.

    Two-Loop O(αsGFmt2){\cal O}(\alpha_sG_Fm_t^2) Corrections to the Fermionic Decay Rates of the Standard-Model Higgs Boson

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    Low- and intermediate mass Higgs bosons decay preferably into fermion pairs. The one-loop electroweak corrections to the respective decay rates are dominated by a flavour-independent term of O(GFmt2){\cal O}(G_Fm_t^2). We calculate the two-loop gluon correction to this term. It turns out that this correction screens the leading high-mtm_t behaviour of the one-loop result by roughly 10\%. We also present the two-loop QCD correction to the contribution induced by a pair of fourth-generation quarks with arbitrary masses. As expected, the inclusion of the QCD correction considerably reduces the renormalization-scheme dependence of the prediction.Comment: 14 pages, latex, figures 2-5 appended, DESY 94-08
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