A systematic review of neuroprotective strategies after cardiac arrest: from bench to bedside (Part I - Protection via specific pathways).

Neurocognitive deficits are a major source of morbidity in survivors of cardiac arrest. Treatment options that could be implemented either during cardiopulmonary resuscitation or after return of spontaneous circulation to improve these neurological deficits are limited. We conducted a literature review of treatment protocols designed to evaluate neurologic outcome and survival following cardiac arrest with associated global cerebral ischemia. The search was limited to investigational therapies that were utilized to treat global cerebral ischemia associated with cardiac arrest. In this review we discuss potential mechanisms of neurologic protection following cardiac arrest including actions of several medical gases such as xenon, argon, and nitric oxide. The 3 included mechanisms are: 1. Modulation of neuronal cell death; 2. Alteration of oxygen free radicals; and 3. Improving cerebral hemodynamics. Only a few approaches have been evaluated in limited fashion in cardiac arrest patients and results show inconclusive neuroprotective effects. Future research focusing on combined neuroprotective strategies that target multiple pathways are compelling in the setting of global brain ischemia resulting from cardiac arrest

Comparison of web-based and face-to-face interviews for application to an anesthesiology training program: a pilot study.

ObjectiveThis study compared admission rates to a United States anesthesiology residency program for applicants completing face-to-face versus web-based interviews during the admissions process. We also explored factors driving applicants to select each interview type.MethodsThe 211 applicants invited to interview for admission to our anesthesiology residency program during the 2014-2015 application cycle were participants in this pilot observational study. Of these, 141 applicants selected face-to-face interviews, 53 applicants selected web-based interviews, and 17 applicants declined to interview. Data regarding applicants' reasons for selecting a particular interview type were gathered using an anonymous online survey after interview completion. Residency program admission rates and survey answers were compared between applicants completing face-to-face versus web-based interviews.ResultsOne hundred twenty-seven (75.1%) applicants completed face-to-face and 42 (24.9%) completed web-based interviews. The admission rate to our residency program was not significantly different between applicants completing face-to-face versus web-based interviews. One hundred eleven applicants completed post-interview surveys. The most common reasons for selecting web-based interviews were conflict of interview dates between programs, travel concerns, or financial limitations. Applicants selected face-to-face interviews due to a desire to interact with current residents, or geographic proximity to the residency program.ConclusionsThese results suggest that completion of web-based interviews is a viable alternative to completion of face-to-face interviews, and that choice of interview type does not affect the rate of applicant admission to the residency program. Web-based interviews may be of particular interest to applicants applying to a large number of programs, or with financial limitations

Cosmology and Astrophysics from Relaxed Galaxy Clusters II: Cosmological Constraints

We present cosmological constraints from measurements of the gas mass fraction, $f_{gas}$, for massive, dynamically relaxed galaxy clusters. Our data set consists of Chandra observations of 40 such clusters, identified in a comprehensive search of the Chandra archive, as well as high-quality weak gravitational lensing data for a subset of these clusters. Incorporating a robust gravitational lensing calibration of the X-ray mass estimates, and restricting our measurements to the most self-similar and accurately measured regions of clusters, significantly reduces systematic uncertainties compared to previous work. Our data for the first time constrain the intrinsic scatter in $f_{gas}$, $(7.4\pm2.3)$% in a spherical shell at radii 0.8-1.2 $r_{2500}$, consistent with the expected variation in gas depletion and non-thermal pressure for relaxed clusters. From the lowest-redshift data in our sample we obtain a constraint on a combination of the Hubble parameter and cosmic baryon fraction, $h^{3/2}\Omega_b/\Omega_m=0.089\pm0.012$, that is insensitive to the nature of dark energy. Combined with standard priors on $h$ and $\Omega_b h^2$, this provides a tight constraint on the cosmic matter density, $\Omega_m=0.27\pm0.04$, which is similarly insensitive to dark energy. Using the entire cluster sample, extending to $z>1$, we obtain consistent results for $\Omega_m$ and interesting constraints on dark energy: $\Omega_\Lambda=0.65^{+0.17}_{-0.22}$ for non-flat $\Lambda$CDM models, and $w=-0.98\pm0.26$ for flat constant-$w$ models. Our results are both competitive and consistent with those from recent CMB, SNIa and BAO data. We present constraints on models of evolving dark energy from the combination of $f_{gas}$ data with these external data sets, and comment on the possibilities for improved $f_{gas}$ constraints using current and next-generation X-ray observatories and lensing data. (Abridged)Comment: 25 pages, 14 figures, 8 tables. Accepted by MNRAS. Code and data can be downloaded from http://www.slac.stanford.edu/~amantz/work/fgas14/ . v2: minor fix to table 1, updated bibliograph

Cosmology and astrophysics from relaxed galaxy clusters - IV: Robustly calibrating hydrostatic masses with weak lensing

This is the fourth in a series of papers studying the astrophysics and cosmology of massive, dynamically relaxed galaxy clusters. Here, we use measurements of weak gravitational lensing from the Weighing the Giants project to calibrate Chandra X-ray measurements of total mass that rely on the assumption of hydrostatic equilibrium. This comparison of X-ray and lensing masses provides a measurement of the combined bias of X-ray hydrostatic masses due to both astrophysical and instrumental sources. Assuming a fixed cosmology, and within a characteristic radius (r_2500) determined from the X-ray data, we measure a lensing to X-ray mass ratio of 0.96 +/- 9% (stat) +/- 9% (sys). We find no significant trends of this ratio with mass, redshift or the morphological indicators used to select the sample. In accordance with predictions from hydro simulations for the most massive, relaxed clusters, our results disfavor strong, tens-of-percent departures from hydrostatic equilibrium at these radii. In addition, we find a mean concentration of the sample measured from lensing data of c_200 = $3.0_{-1.8}^{+4.4}$. Anticipated short-term improvements in lensing systematics, and a modest expansion of the relaxed lensing sample, can easily increase the measurement precision by 30--50%, leading to similar improvements in cosmological constraints that employ X-ray hydrostatic mass estimates, such as on Omega_m from the cluster gas mass fraction.Comment: 13 pages. Submitted to MNRAS. Comments welcom

ERTS and aircraft multispectral scanner digital data users manual

There are no author-identified significant results in this report

Automated Screening of Microtubule Growth Dynamics Identifies MARK2 as a Regulator of Leading Edge Microtubules Downstream of Rac1 in Migrating Cells

Polarized microtubule (MT) growth in the leading edge is critical to directed cell migration, and is mediated by Rac1 GTPase. To find downstream targets of Rac1 that affect MT assembly dynamics, we performed an RNAi screen of 23 MT binding and regulatory factors and identified RNAi treatments that suppressed changes in MT dynamics induced by constitutively activated Rac1. By analyzing fluorescent EB3 dynamics with automated tracking, we found that RNAi treatments targeting p150glued, APC2, spastin, EB1, Op18, or MARK2 blocked Rac1-mediated MT growth in lamellipodia. MARK2 was the only protein whose RNAi targeting additionally suppressed Rac1 effects on MT orientation in lamellipodia, and thus became the focus of further study. We show that GFP-MARK2 rescued effects of MARK2 depletion on MT growth lifetime and orientation, and GFP-MARK2 localized in lamellipodia in a Rac1-activity-dependent manner. In a wound-edge motility assay, MARK2-depleted cells failed to polarize their centrosomes or exhibit oriented MT growth in the leading edge, and displayed defects in directional cell migration. Thus, automated image analysis of MT assembly dynamics identified MARK2 as a target regulated downstream of Rac1 that promotes oriented MT growth in the leading edge to mediate directed cell migration