183 research outputs found
Molecular properties of (U)LIRGs: CO, HCN, HNC and HCO+
The observed molecular properties of a sample of FIR-luminous and OH
megamaser (OH-MM) galaxies have been investigated. The ratio of high and
low-density tracer lines is found to be determined by the progression of the
star formation in the system. The HCO+/HCN and HCO+/HNC line ratios are good
proxies for the density of the gas, and PDR and XDR sources can be
distinguished using the HNC/HCN line ratio. The properties of the OH-MM sources
in the sample can be explained by PDR chemistry in gas with densities higher
than 10^5.5 cm^-3, confirming the classical OH-MM model of IR pumped
amplification with (variable) low gains.Comment: 5 pages, 2 figures, to appear in: IAU Symposium 242 Astrophysical
Masers and their Environment
Evolution of the ISM in Luminous IR Galaxies
Molecules that trace the high-density regions of the interstellar medium may
be used to evaluate the changing physical and chemical environment during the
ongoing nuclear activity in (Ultra-)Luminous Infrared Galaxies. The changing
ratios of the HCN(1-0), HNC(1-0), HCO+(1-0), CN(1-0) and CN(2-1), and CS(3-2)
transitions were compared with the HCN(1-0)/CO(1-0) ratio, which is proposed to
represent the progression time scale of the starburst. These diagnostic
diagrams were interpreted using the results of theoretical modeling using a
large physical and chemical network to describe the state of the nuclear ISM in
the evolving galaxies. Systematic changes are seen in the line ratios as the
sources evolve from early stage for the nuclear starburst (ULIRGs) to later
stages. These changes result from changing environmental conditions and
particularly from the lowering of the average density of the medium. A
temperature rise due to mechanical heating of the medium by feedback explains
the lowering of the ratios at later evolutionary stages. Infrared pumping may
affect the CN and HNC line ratios during early evolutionary stages. Molecular
transitions display a behavior that relates to changes of the environment
during an evolving nuclear starburst. Molecular properties may be used to
designate the evolutionary stage of the nuclear starburst. The HCN(1-0)/CO(1-0)
and HCO+(1-0)/HCN(1-0) ratios serve as indicators of the time evolution of the
outburst.Comment: To be published in Astronomy and Astrophysics - 11 pages, 9 figures,
1 tabl
The irradiated ISM of ULIRGs
The nuclei of ULIRGs harbor massive young stars, an accreting central black
hole, or both. Results are presented for molecular gas that is exposed to
X-rays (1-100 keV, XDRs) and far-ultraviolet radiation (6-13.6 eV, PDRs).
Attention is paid to species like HCO+, HCN, HNC, OH, H2O and CO. Line ratios
of HCN/HCO+ and HNC/HCN discriminate between PDRs and XDRs. Very high J (>10)
CO lines, observable with HIFI/Herschel, discriminate very well between XDRs
and PDRs. In XDRs, it is easy to produce large abundances of warm (T>100 K) H2O
and OH. In PDRs, only OH is produced similarly well.Comment: 5 pages, 6 figures, to appear in: IAU Symposium 242 Astrophysical
Masers and their Environment
Mechanical feedback in the molecular ISM of luminous IR galaxies
Aims: Molecular emission lines originating in the nuclei of luminous
infra-red galaxies are used to determine the physical properties of the nuclear
ISM in these systems.
Methods: A large observational database of molecular emission lines is
compared with model predictions that include heating by UV and X-ray radiation,
mechanical heating, and the effects of cosmic rays.
Results: The observed line ratios and model predictions imply a separation of
the observedsystems into three groups: XDRs, UV-dominated high-density (n>=10^5
cm-3) PDRs, and lower-density (n=10^4.5 cm-3) PDRs that are dominated by
mechanical feedback.
Conclusions: The division of the two types of PDRs follows naturally from the
evolution of the star formation cycle of these sources, which evolves from
deeply embedded young stars, resulting in high-density (n>=10^5 cm-3) PDRs, to
a stage where the gas density has decreased (n=10^4.5 cm-3) and mechanical
feedback from supernova shocks dominates the heating budget.Comment: 4 pages, 3 figures, published as Letter to the Editor in A&A (see
http://www.aanda.org/articles/aa/abs/2008/34/aa10327-08/aa10327-08.html
Molecules as tracers of galaxy evolution: an EMIR survey. I. Presentation of the data and first results
We investigate the molecular gas properties of a sample of 23 galaxies in
order to find and test chemical signatures of galaxy evolution and to compare
them to IR evolutionary tracers. Observation at 3 mm wavelengths were obtained
with the EMIR broadband receiver, mounted on the IRAM 30 m telescope on Pico
Veleta, Spain. We compare the emission of the main molecular species with
existing models of chemical evolution by means of line intensity ratios
diagrams and principal component analysis. We detect molecular emission in 19
galaxies in two 8 GHz-wide bands centred at 88 and 112 GHz. The main detected
transitions are the J=1-0 lines of CO, 13CO, HCN, HNC, HCO+, CN, and C2H. We
also detect HC3N J=10-9 in the galaxies IRAS 17208, IC 860, NGC 4418, NGC 7771,
and NGC 1068. The only HC3N detections are in objects with HCO+/HCN<1 and warm
IRAS colours. Galaxies with the highest HC3N/HCN ratios have warm IRAS colours
(60/100 {\mu}m>0.8). The brightest HC3N emission is found in IC 860, where we
also detect the molecule in its vibrationally excited state.We find low HNC/HCN
line ratios (<0.5), that cannot be explained by existing PDR or XDR chemical
models. Bright HC3N emission in HCO+-faint objects may imply that these are not
dominated by X-ray chemistry. Thus the HCN/HCO+ line ratio is not, by itself, a
reliable tracer of XDRs. Bright HC3N and faint HCO+ could be signatures of
embedded starformation, instead of AGN activity
Insights into the regulation of DMSP synthesis in the diatom Thalassiosira pseudonana through APR activity, proteomics and gene expression analyses on cells acclimating to changes in salinity, light and nitrogen
Despite the importance of dimethylsulphoniopropionate (DMSP) in the global sulphur cycle and climate regulation, the biological pathways underpinning its synthesis in marine phytoplankton remain poorly understood. The intracellular concentration of DMSP increases with increased salinity, increased light intensity and nitrogen starvation in the diatom Thalassiosira pseudonana. We used these conditions to investigate DMSP synthesis at the cellular level via analysis of enzyme activity, gene expression and proteome comparison. The activity of the key sulphur assimilatory enzyme, adenosine 5′- phosphosulphate reductase was not coordinated with increasing intracellular DMSP concentration. Under all three treatments coordination in the expression of sulphur assimilation genes was limited to increases in sulphite reductase transcripts. Similarly, proteomic 2D gel analysis only revealed an increase in phosphoenolpyruvate carboxylase following increases in DMSP concentration. Our findings suggest that increased sulphur assimilation might not be required for increased DMSP synthesis, instead the availability of carbon and nitrogen substrates may be important in the regulation of this pathway. This contrasts with the regulation of sulphur metabolism in higher plants, which generally involves upregulation of several sulphur assimilatory enzymes. In T. pseudonana changes relating to sulphur metabolism were specific to the individual treatments and, given that little coordination was seen in transcript and protein responses across the three growth conditions, different patterns of regulation might be responsible for the increase in DMSP concentration seen under each treatment
Gravitational lensing reveals extreme dust-obscured star formation in quasar host galaxies
We have observed 104 gravitationally-lensed quasars at with
Herschel/SPIRE, the largest such sample ever studied. By targeting
gravitational lenses, we probe intrinsic far-infrared (FIR) luminosities and
star formation rates (SFRs) more typical of the population than the extremely
luminous sources that are otherwise accessible. We detect 72 objects with
Herschel/SPIRE and find 66 percent (69 sources) of the sample have spectral
energy distributions (SEDs) characteristic of dust emission. For 53 objects
with sufficiently constrained SEDs, we find a median effective dust temperature
of K. By applying the radio-infrared correlation, we find no
evidence for an FIR excess which is consistent with star-formation-heated dust.
We derive a median magnification-corrected FIR luminosity of
and median SFR of
for 94 quasars with redshifts. We
find percent of our sample have FIR properties similar to typical
dusty star-forming galaxies at and a range of SFRs for our sample as a whole. These results are in line with
current models of quasar evolution and suggests a coexistence of dust-obscured
star formation and AGN activity is typical of most quasars. We do not find a
statistically-significant difference in the FIR luminosities of quasars in our
sample with a radio excess relative to the radio-infrared correlation.
Synchrotron emission is found to dominate at FIR wavelengths for percent
of those sources classified as powerful radio galaxies.Comment: 47 pages, 89 figures, accepted for publication in MNRA
HSV Neutralization by the Microbicidal Candidate C5A
Genital herpes is a major risk factor in acquiring human immunodeficiency virus type-1 (HIV-1) infection and is caused by both Herpes Simplex virus type 1 (HSV-1) and HSV-2. The amphipathic peptide C5A, derived from the non-structural hepatitis C virus (HCV) protein 5A, was shown to prevent HIV-1 infection but neither influenza nor vesicular stomatitis virus infections. Here we investigated the antiviral function of C5A on HSV infections. C5A efficiently inhibited both HSV-1 and HSV-2 infection in epithelial cells in vitro as well as in an ex vivo epidermal infection model. C5A destabilized the integrity of the viral HSV membrane. Furthermore, drug resistant HSV strains were inhibited by this peptide. Notably, C5A-mediated neutralization of HSV-1 prevented HIV-1 transmission. An in vitro HIV-1 transmigration assay was developed using primary genital epithelial cells and HSV infection increased HIV-1 transmigration. Treatment with C5A abolished HIV-1 transmigration by preventing HSV infection and by preserving the integrity of the genital epithelium that was severely compromised by HSV infection. In conclusion, this study demonstrates that C5A represents a multipurpose microbicide candidate, which neutralizes both HIV-1 and HSV, and which may interfere with HIV-1 transmission through the genital epithelium
Signal Peptide-Dependent Inhibition of MHC Class I Heavy Chain Translation by Rhesus Cytomegalovirus
The US2-11 region of human and rhesus cytomegalovirus encodes a conserved family of glycoproteins that inhibit MHC-I assembly with viral peptides, thus preventing cytotoxic T cell recognition. Since HCMV lacking US2-11 is no longer able to block assembly and transport of MHC-I, we examined whether this is also observed for RhCMV lacking the corresponding region. Unexpectedly, recombinant RhCMV lacking US2-11 was still able to inhibit MHC-I expression in infected fibroblasts, suggesting the presence of an additional MHC-I evasion mechanism. Progressive deletion analysis of RhCMV-specific genomic regions revealed that MHC-I expression is fully restored upon additional deletion of rh178. The protein encoded by this RhCMV-specific open reading frame is anchored in the endoplasmic reticulum membrane. In the presence of rh178, RhCMV prevented MHC-I heavy chain (HC) expression, but did not inhibit mRNA transcription or association of HC mRNA with translating ribosomes. Proteasome inhibitors stabilized a HC degradation intermediate in the absence of rh178, but not in its presence, suggesting that rh178 prevents completion of HC translation. This interference was signal sequence-dependent since replacing the signal peptide with that of CD4 or murine HC rendered human HCs resistant to rh178. We have identified an inhibitor of antigen presentation encoded by rhesus cytomegalovirus unique in both its lack of homology to any other known protein and in its mechanism of action. By preventing signal sequence-dependent HC translocation, rh178 acts prior to US2, US3 and US11 which attack MHC-I proteins after protein synthesis is completed. Rh178 is the first viral protein known to interfere at this step of the MHC-I pathway, thus taking advantage of the conserved nature of HC leader peptides, and represents a new mechanism of translational interference
Nuclear localised more sulphur accumulation1 epigenetically regulates sulphur homeostasis in Arabidopsis thaliana
Sulphur (S) is an essential element for all living organisms. The uptake, assimilation and metabolism of S in plants are well studied. However, the regulation of S homeostasis remains largely unknown. Here, we report on the identification and characterisation of the more sulphur accumulation1 (msa1-1) mutant. The MSA1 protein is localized to the nucleus and is required for both S adenosylmethionine (SAM) production and DNA methylation. Loss of function of the nuclear localised MSA1 leads to a reduction in SAM in roots and a strong S-deficiency response even at ample S supply, causing an over- accumulation of sulphate, sulphite, cysteine and glutathione. Supplementation with SAM suppresses this high S phenotype. Furthermore, mutation of MSA1 affects genome-wide DNA methylation, including the methylation of S-deficiency responsive genes. Elevated S accumulation in msa1-1 requires the increased expression of the sulphate transporter genes SULTR1;1 and SULTR1;2 which are also differentially methylated in msa1-1. Our results suggest a novel function for MSA1 in the nucleus in regulating SAM biosynthesis and maintaining S homeostasis epigenetically via DNA methylation
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