Article 4: Bank Deposits and Collections

Montana Law and the Uniform Commercial Code: Article 4: Bank Deposits and Collection

Montana Bar Association Activities

Montana Bar Association Activitie

James v. V.K.V. Lumber Co., 145 Mont. 466, 401 P.2d 282 (1965)

James v. V.K.V. Lumber Co

James v. V.K.V. Lumber Co., 145 Mont. 466, 401 P.2d 282 (1965)

James v. V.K.V. Lumber Co

Article 4: Bank Deposits and Collections

Montana Law and the Uniform Commercial Code: Article 4: Bank Deposits and Collection

Requirements for Human Respiratory Syncytial Virus Glycoproteins in Assembly and Egress from Infected Cells

Human respiratory syncytial virus (HRSV) is an enveloped RNA virus that assembles and buds from the plasma membrane of infected cells. The ribonucleoprotein complex (RNP) must associate with the viral matrix protein and glycoproteins to form newly infectious particles prior to budding. The viral proteins involved in HRSV assembly and egress are mostly unexplored. We investigated whether the glycoproteins of HRSV were involved in the late stages of viral replication by utilizing recombinant viruses where each individual glycoprotein gene was deleted and replaced with a reporter gene to maintain wild-type levels of gene expression. These engineered viruses allowed us to study the roles of the glycoproteins in assembly and budding in the context of infectious virus. Microscopy data showed that the F glycoprotein was involved in the localization of the glycoproteins with the other viral proteins at the plasma membrane. Biochemical analyses showed that deletion of the F and G proteins affected incorporation of the other viral proteins into budded virions. However, efficient viral release was unaffected by the deletion of any of the glycoproteins individually or in concert. These studies attribute a novel role to the F and G proteins in viral protein localization and assembly

Transcriptional Termination Modulated by Nucleotides Outside the Characterized Gene End Sequence of Respiratory Syncytial Virus

AbstractThe genes of respiratory syncytial (RS) virus are transcribed sequentially by the viral RNA polymerase from a single 3′-proximal promoter. Polyadenylation and termination are directed by a sequence at the end of each gene, after which the polymerase crosses an intergenic region and reinitiates at the start sequence of the next gene. The 10 viral genes have different gene end sequences and different termination efficiencies, which allow for regulation of gene expression, since termination of each gene is required for initiation of the downstream gene. RNA sequences within the previously characterized 13 nucleotide gene end, including a conserved sequence 3′-UCAAU-5′ and a tract of U residues, are important for termination. In this study, two additional sequence elements outside of the 13 nucleotide gene end were found to modulate termination efficiency: the A residue upstream of the 3′-UCAAU-5′ sequence, and the first nucleotide of the intergenic region when it follows a U4 tract

Cells that express all five proteins of vesicular stomatitis virus from cloned cDNAs support replication, assembly, and budding of defective interfering particles

An alternative approach to structurefunction analysis of vesicular stomatitis virus (VSV) gene products and their interactions with one another during each phase of the viral life cycle is described. We showed previously by using the vaccinia viruslT7 RNA polymerase expression system that when cells expressing the nucleocapsid protein (N), the phosphoprotein (NS), and the large polymerase protein (L) of VSV were superinfected with defective interfering (DI) particles, rapid and efflicient replication and amplification of DI particle RNA occurred. Here, we demonstrate that all five VSV proteins can be expressed simultaneously when cells are cotransfected with plasmids containing the matrix protein (M) gene and the glycoprotein (G) gene of VSV in addition to plasmids containing the genes for the N, NS, and L proteins. When cells coexpressing all five VSV proteins were superinfected with DI particles, which because of their defectiveness are unable to express any viral proteins or to replicate, DI particle replication, assembly, and budding were observed and infectious DI particles were released into the culture fluids. Omission of either the M or G protein expression resulted in no DI particle budding. The vector-supported DI particles were similar in size and morphology to the authentic DI particles generated from cells coinfected with DI particles and helper VSV and their infectivity could be blocked by anti-VSV or anti-G antiserum. The successful replication, assembly, and budding of DI particles from cells expressing all five VSV proteins from cloned cDNAs provide a powerful approach for detailed structure-function analysis of the VSV gene products in each step of the replicative cycle of the virus

Remote sensing study of land use and sedimentation in the Ross Barnett Reservoir, Jackson, Mississippi area

This multi-year study is aimed at focusing on the recognition of sediment and other affluents in a selected area of the Ross Barnett Reservoir. The principle objectives are the determination of land use types, effect of land use on erosion, and the correlation of sediment with land use in the area. The I2S multi-band imagery was employed in conjunction with ground truth data for both water and land use studies. The selected test site contains approximately forty square miles including forest, open land, and water in addition to residential and recreational areas