235 research outputs found
The use of shape-selective stationary phases in GC. Part 1: Cyclodextrin phases
ABSTRACT -Cyclodextrin phases are one of the most interesting geometry selective stationary phase in GC. 'beyare outstanding in their ability to separate isomeric and enantiomeric compounds. Two examples of the separation of cyclic compounds on cyclodextrins are given. The combination of chiral and achiral phases in series coupled columns is a useful tool for complicate separation and identification problems
Alteration of the in vivo nicotinic receptor density in ADNFLE patients: a PET study
Nicotinic acetylcholine receptors (nAChRs) are involved in a familial form of frontal lobe epilepsy, autosomal dominant nocturnal frontal lobe epilepsy (ADNFLE). In several ADNFLE families, mutations were identified in the nAChR α4 or ÎČ2 subunit, which together compose the main cerebral nAChR. Electrophysiological assessment using in vitro expression systems indicated a gain of function of the mutant receptors. However the precise mechanisms by which they contribute to the pathogenesis of a focal epilepsy remain obscure, especially since α4ÎČ2 nAChRs are known to be widely distributed within the entire brain. PET study using [18F]-F-A-85380, a high affinity agonist at the α4ÎČ2 nAChRs, allows the determination of the regional distribution and density of the nAChRs in healthy volunteers and in ADNFLE patients, thus offering a unique opportunity to investigate some in vivo consequences of the molecular defect. We have assessed nAChR distribution in eight non-smoking ADNFLE patients (from five families) bearing an identified mutation in nAChRs and in seven age-matched non-smoking healthy volunteers using PET and [18F]-F-A-85380. Parametric images of volume of distribution (Vd) were generated as the ratio of tissue to plasma radioactivities. The images showed a clear difference in the pattern of the nAChR density in the brains of the patients compared to the healthy volunteers. Vd values revealed a significant increase (between 12 and 21%, P < 0.05) in the ADNFLE patients in the mesencephalon, the pons and the cerebellum when compared to control subjects. Statistical parametric mapping (SPM) was then used to better analyse subtle regional differences. This analysis confirmed clear regional differences between patients and controls: patients had increased nAChR density in the epithalamus, ventral mesencephalon and cerebellum, but decreased nAChR density in the right dorsolateral prefrontal region. In five patients who underwent an additional [18F]-fluorodeoxyglucose (FDG) PET experiment, hypometabolism was observed in the neighbouring area of the right orbitofrontal cortex. The demonstration of a regional nAChR density decrease in the prefrontal cortex, despite the known distribution of these receptors throughout the cerebral cortex, is consistent with a focal epilepsy involving the frontal lobe. We also propose that the nAChR density increase in mesencephalon is involved in the pathophysiology of ADNFLE through the role of brainstem ascending cholinergic systems in arousa
Evaluation of Hylleraas-CI atomic integrals by integration over the coordinates of one electron. IV. An improved algorithm for three-electron kinetic energy integrals
An improved algorithm to evaluate the nonrelativistic three-electron
Hylleraas-Configuration Interaction (Hy-CI) kinetic energy integrals over
Slater orbitals and the Hamiltonian in Hylleraas coordinates is shown. The
resulting analytical expressions are general for all quantum numbers of the
orbitals. From there, the restriction of employing orbitals with quantum
numbers of the above algorithm presented in paper I of this series
has been removed. With the new algorithm it is possible, in the direct
integration method described in this series, to carry out Hy-CI atomic
structure calculations including -, -, , and higher
angular-momentum Slater orbitals and to determine , , , and
states of higher order symmetry.Comment: 32 pages, 14 references, 1 figure and 1 table, Journal of
Mathematical Chemistry, January 201
A semiconductor laser system for the production of antihydrogen
Laser-controlled charge exchange is a promising method for producing cold antihydrogen. Caesium atoms in Rydberg states collide with positrons and create positronium. These positronium atoms then interact with antiprotons, forming antihydrogen. Las er excitation of the caesium atoms is essential to increase the cross section of the charge-exchange collisions. This method was demonstrated in 2004 by the ATRAP collaboration by using an available copper vapour laser. For a second generation of charge-e xchange experiments we have designed a new semiconductor laser system that features several improvements compared to the copper vapour laser. We describe this new laser system and show the results from the excitation of caesium atoms to Rydberg states wit hin the strong magnetic fields in the ATRAP apparatus
Discovery of 3-Formyl-Tyrosine Metabolites from Pseudoalteromonas tunicata through Heterologous Expression
Genome mining and identification of natural product gene clusters typically relies on the presence of canonical nonribosomal polypeptide synthetase (NRPS) or polyketide synthase (PKS) domains. Recently, other condensation enzymes, such as the ATP-grasp ligases, have been recognized as important players in natural product biosynthesis. In this study, sequence based searching for homologues of DdaF, the ATP-grasp amide ligase from dapdiamide biosynthesis, led to the identification of a previously unannotated biosynthetic gene cluster in Pseudoalteromonas tunicata. Heterologous expression of the cluster in Escherichia coli allowed for the production and structure determination of two new 3-formyl tyrosine metabolites.Molecular and Cellular Biolog
DRhigh+CD45RAâ-Tregs Potentially Affect the Suppressive Activity of the Total Treg Pool in Renal Transplant Patients
Recent studies show that regulatory T cells (Tregs) play an essential role in tolerance induction after organ transplantation. In order to examine whether there are differences in the composition of the total CD4+CD127low+/âFoxP3+- Treg cell pool between stable transplant patients and patients with biopsy proven rejection (BPR), we compared the percentages and the functional activity of the different Treg cell subsets (DRhigh+CD45RAâ-Tregs, DRlow+CD45RAâ-Tregs, DRâCD45RAâ-Tregs, DRâCD45RA+-Tregs). All parameters were determined during the three different periods of time after transplantation (0â30 days, 31â1,000 days, >1,000 days). Among 156 transplant patients, 37 patients suffered from BPR. The most prominent differences between rejecting and non-rejecting patients were observed regarding the DRhigh+CD45RAâ-Treg cell subset. Our data demonstrate that the suppressive activity of the total Treg pool strongly depends on the presence of these Treg cells. Their percentage within the total Treg pool strongly decreased after transplantation and remained relatively low during the first year after transplantation in all patients. Subsequently, the proportion of this Treg subset increased again in patients who accepted the transplant and reached a value of healthy non-transplanted subjects. By contrast, in patients with acute kidney rejection, the DRhigh+CD45RAâ-Treg subset disappeared excessively, causing a reduction in the suppressive activity of the total Treg pool. Therefore, both the monitoring of its percentage within the total Treg pool and the monitoring of the HLA-DR MFI of the DR+CD45RAâ-Treg subset may be useful tools for the prediction of graft rejection
Mathematical Properties of a New Levin-Type Sequence Transformation Introduced by \v{C}\'{\i}\v{z}ek, Zamastil, and Sk\'{a}la. I. Algebraic Theory
\v{C}\'{\i}\v{z}ek, Zamastil, and Sk\'{a}la [J. Math. Phys. \textbf{44}, 962
- 968 (2003)] introduced in connection with the summation of the divergent
perturbation expansion of the hydrogen atom in an external magnetic field a new
sequence transformation which uses as input data not only the elements of a
sequence of partial sums, but also explicit estimates
for the truncation errors. The explicit
incorporation of the information contained in the truncation error estimates
makes this and related transformations potentially much more powerful than for
instance Pad\'{e} approximants. Special cases of the new transformation are
sequence transformations introduced by Levin [Int. J. Comput. Math. B
\textbf{3}, 371 - 388 (1973)] and Weniger [Comput. Phys. Rep. \textbf{10}, 189
- 371 (1989), Sections 7 -9; Numer. Algor. \textbf{3}, 477 - 486 (1992)] and
also a variant of Richardson extrapolation [Phil. Trans. Roy. Soc. London A
\textbf{226}, 299 - 349 (1927)]. The algebraic theory of these transformations
- explicit expressions, recurrence formulas, explicit expressions in the case
of special remainder estimates, and asymptotic order estimates satisfied by
rational approximants to power series - is formulated in terms of hitherto
unknown mathematical properties of the new transformation introduced by
\v{C}\'{\i}\v{z}ek, Zamastil, and Sk\'{a}la. This leads to a considerable
formal simplification and unification.Comment: 41 + ii pages, LaTeX2e, 0 figures. Submitted to Journal of
Mathematical Physic
Context specificity of post-error and post-conflict cognitive control adjustments
There has been accumulating evidence that cognitive control can be adaptively regulated by monitoring for processing conflict as an index of online control demands. However, it is not yet known whether top-down control mechanisms respond to processing conflict in a manner specific to the operative task context or confer a more generalized benefit. While previous studies have examined the taskset-specificity of conflict adaptation effects, yielding inconsistent results, controlrelated performance adjustments following errors have been largely overlooked. This gap in the literature underscores recent debate as to whether post-error performance represents a strategic, control-mediated mechanism or a nonstrategic consequence of attentional orienting. In the present study, evidence of generalized control following both high conflict correct trials and errors was explored in a task-switching paradigm. Conflict adaptation effects were not found to generalize across tasksets, despite a shared response set. In contrast, post-error slowing effects were found to extend to the inactive taskset and were predictive of enhanced post-error accuracy. In addition, post-error performance adjustments were found to persist for several trials and across multiple task switches, a finding inconsistent with attentional orienting accounts of post-error slowing. These findings indicate that error-related control adjustments confer a generalized performance benefit and suggest dissociable mechanisms of post-conflict and post-error control. © 2014 Forster, Cho
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