Magnetic fields around evolved stars: further observations of H2_2O maser polarization

We aim to detect the magnetic field and infer its properties around four AGB stars using H$_2$O maser observations. The sample we observed consists of the following sources: the semi-regular variable RT Vir and the Mira variables AP Lyn, IK Tau, and IRC+60370. We observed the 6$_{1,6}-5_{2,3}$ H$_2$O maser rotational transition, in full-polarization mode, to determine its linear and circular polarization. Based on the Zeeman effect, one can infer the properties of the magnetic field from the maser polarization analysis. We detected a total of 238 maser features, in three of the four observed sources. No masers were found toward AP Lyn. The observed masers are all located between 2.4 and 53.0 AU from the stars. Linear and circular polarization was found in 18 and 11 maser features, respectively. We more than doubled the number of AGB stars in which magnetic field has been detected from H$_2$O maser polarization, as our results confirm the presence of fields around IK Tau, RT Vir and IRC+60370. The strength of the field along the line of sight is found to be between 47 and 331 mG in the H$_2$O maser region. Extrapolating this result to the surface of the stars, assuming a toroidal field ($\propto$ r$^{-1}$), we find magnetic fields of 0.3-6.9 G on the stellar surfaces. If, instead of a toroidal field, we assume a poloidal field ($\propto$ r$^{-2}$), then the extrapolated magnetic field strength on the stellar surfaces are in the range between 2.2 and $\sim$115 G. Finally, if a dipole field ($\propto$ r$^{-3}$) is assumed, the field strength on the surface of the star is found to be between 15.8 and $\sim$1945 G. The magnetic energy of our sources is higher than the thermal and kinetic energy in the H$_2$O maser region of this class of objects. This leads us to conclude that, indeed, magnetic fields probably play an important role in shaping the outflows of evolved stars. (abridged)Comment: 15 pages, 5 figures, 7 tables. Accepted for publication in A&

Feeding and foraging ecology of Trindade petrels Pterodroma arminjoniana during the breeding period in the South Atlantic Ocean

Seabirds breeding in tropical environments experience high energetic demands, when foraging in an oligotrophic environment. The globally threatened Trindade petrel Pterodroma arminjoniana has its largest colony in Trindade Island (20°30′S–29°19′W) inside the oligotrophic South Atlantic Subtropical Gyre. Diet sampling methods, geolocator tracking and stable isotope analysis were used to describe its diet, compare foraging trips and distributions, and assess temporal variations in the trophic niche throughout the breeding period. Diet consisted mainly of squid and fish. The high species diversity and wide range of prey sizes consumed suggests the use of multiple foraging techniques. Stable isotope mixing models confirm that Trindade petrels rely mainly on squid throughout the breeding period. Its broad isotopic niche seems to reflect both a diverse diet and foraging range, since birds can reach up to 3335 km from the colony. Isotopic niche showed limited variation even in an 8-year interval, apparently due to oceanographic stability, although changes in the isotopic niche have demonstrated an adjustment to different conditions in different seasons. Petrels change foraging areas and prey during the breeding period: pre-incubating birds use more productive areas west of Trindade Island and obtain low trophic position prey; incubating petrels perform longer trips southward to consume prey of high trophic position; and chick-rearing petrels use areas around the island. These results demonstrate that to deal with high demand breeding in a colony surrounded by oligotrophic waters, Trindade petrels need to explore wide foraging areas and utilize a diverse diet, besides adjusting trophic niche according to breeding stage

The creeping motion of immiscible drops through a converging/diverging tube

Results of experiments on the low-Reynolds-number flow of liquid drops through a horizontal circular tube with a diameter that varies sinusoidally with axial position are reported. Measurements of the contribution of the drop to the local pressure gradient and the relative velocity of the drop are correlated with the time-dependent drop shape. Both Newtonian and viscoelastic suspending fluids are considered. The viscosity ratio, volumetric flow rate and drop size are varied in the experiment, and both neutrally buoyant and non-neutrally buoyant drops are studied. Comparison with previous results for a straight-wall tube shows that the influence of the tube boundary geometry on the drop shape is substantial, but the qualitative effect of the tube shape depends strongly on the relative importance of viscous forces compared to interfacial tension for the particular experiment. For Newtonian fluids, two modes of drop breakup, which are distinguished by the magnitude of the viscosity ratio, are observed. When the suspending fluid is viscoelastic, both shear-thinning and time-dependent rheological effects are present

Odorant-Binding Proteins of the Malaria Mosquito Anopheles funestus sensu stricto

Background The mosquito Anopheles funestus is one of the major malaria vector species in sub-Saharan Africa. Olfaction is essential in guiding mosquito behaviors. Odorant-binding proteins (OBPs) are highly expressed in insect olfactory tissues and involved in the first step of odorant reception. An improved understanding of the function of malaria mosquito OBPs may contribute to identifying new attractants/repellents and assist in the development of more efficient and environmentally friendly mosquito controlling strategies. Methodology In this study, a large screening of over 50 ecologically significant odorant compounds led to the identification of 12 ligands that elicit significant electroantennographic (EAG) responses from An. funestus female antennae. To compare the absolute efficiency/potency of these chemicals, corrections were made for differences in volatility by determining the exact amount in a stimulus puff. Fourteen AfunOBP genes were cloned and their expression patterns were analyzed. AfunOBP1, 3, 7, 20 and 66 showed olfactory tissue specificity by reverse transcriptase PCR (RT-PCR). Quantitative real-time PCR (qRT-PCR) analysis showed that among olfactory-specific OBPs, AfunOBP1 and 3 are the most enriched OBPs in female antennae. Binding assay experiments showed that at pH 7, AfunOBP1 significantly binds to 2-undecanone, nonyl acetate, octyl acetate and 1-octen-3-ol but AfunOBP3, which shares 68% identify with AfunOBP1 at amino acid level, showed nearly no binding activity to the selected 12 EAG-active odorant compounds. Conclusion This work presents for the first time a study on the odorants and OBPs of the malaria vector mosquito An. funestus, which may provide insight into the An. funestus olfactory research, assist in a comparative study between major malaria mosquitoes An. gambiae and An. funestus olfactory system, and help developing new mosquito control strategies to reduce malaria transmission

Formation of Nanopillar Arrays in Ultrathin Viscous Films: The Critical Role of Thermocapillary Stresses

Experiments by several groups during the past decade have shown that a molten polymer nanofilm subject to a large transverse thermal gradient undergoes spontaneous formation of periodic nanopillar arrays. The prevailing explanation is that coherent reflections of acoustic phonons within the film cause a periodic modulation of the radiation pressure which enhances pillar growth. By exploring a deformational instability of particular relevance to nanofilms, we demonstrate that thermocapillary forces play a crucial role in the formation process. Analytic and numerical predictions show good agreement with the pillar spacings obtained in experiment. Simulations of the interface equation further determine the rate of pillar growth of importance to technological applications.Comment: 5 pages, 4 figure

Association of PET-measured myocardial flow reserve with echocardiography-estimated pulmonary artery systolic pressure in patients with hypertrophic cardiomyopathy

BackgroundPulmonary hypertension (PH) is a known complication of HCM and is a strong predictor of mortality. We aim to investigate the relationship between microvascular dysfunction measured by quantitative PET and PH in HCM patients.MethodsEighty-nine symptomatic HCM patients were included in the study. Each patient underwent two 20-min 13N-NH3 dynamic PET scans for rest and stress conditions, respectively. A 2-tissue irreversible compartmental model was used to fit the segments time activity curves for estimating segmental and global myocardial blood flow (MBF) and myocardial flow reserve (MFR). Echocardiographic derived PASP was utilized to estimate PH.ResultsPatients were categorized into two groups across PASP: PH (PASP > 36 mmHg) and no-PH (PASP ≤ 36 mmHg). patients with PH had larger left atrium, ratio of higher inflow early diastole (E) and atrial contraction (A) waves, E/A, and ratio of inflow and peak early diastolic waves, E/e', significantly reduced global stress MBF (1.85 ± 0.52 vs. 2.13 ± 0.56 ml/min/g; p = 0.024) and MFR (2.21 ± 0.57 vs. 2.62 ± 0.75; p = 0.005), while the MBFs at rest between the two groups were similar. There were significant negative correlations between global stress MBF/MFR and PASP (stress MBF: r = -0.23, p = 0.03; MFR: r = -0.32, p = 0.002); for regional MBF and MFR measurements, the highest linear correlation coefficients were observed in the septal wall (stress MBF: r = -0.27, p = 0.01; MFR: r = -0.31, p = 0.003). Global MFR was identified to be independent predictor for PH in multivariate regression analysis.ConclusionEchocardiography-derived PASP is negatively correlated with global MFR measured by 13N-NH3 dynamic PET. Global MFR is suggested to be an index of PH in HCM patients.</div

Extrusion of the C-terminal helix in navel orangeworm moth pheromone-binding protein (AtraPBP1) controls pheromone binding

The navel orangeworm, Amyelois transitella (Walker), is an agricultural insect pest that can be controlled by disrupting male–female communication with sex pheromones, a technique known as mating disruption. Insect pheromone-binding proteins (PBPs) provide fast transport of hydrophobic pheromones through aqueous sensillar lymph and promote sensitive delivery of pheromones to receptors. Here we present a mutational analysis on a PBP from A. transitella (AtraPBP1) to evaluate how the C-terminal helix in this protein controls pheromone binding as a function of pH. Pheromone binds tightly to AtraPBP1 at neutral pH, but the binding is much weaker at pH below 5. Deletion of the entire C-terminal helix (residues 129–142) causes more than 100-fold increase in pheromone-binding affinity at pH 5 and only a 1.5-fold increase at pH 7. A similar pH-dependent increase in pheromone binding is also seen for the H80A/H95A double mutant that promotes extrusion of the C-terminal helix by disabling salt bridges at each end of the helix. The single mutants (H80A and H95A) also exhibit pheromone binding at pH below 5, but with ∼2-fold weaker affinity. NMR and circular dichroism data demonstrate a large overall structural change in each of these mutants at pH 4.5, indicating an extrusion of the C-terminal helix that profoundly affects the overall structure of the low pH form. Our results confirm that sequestration of the C-terminal helix at low pH as seen in the recent NMR structure may serve to block pheromone binding. We propose that extrusion of these C-terminal residues at neutral pH (or by the mutations in this study) exposes a hydrophobic cleft that promotes high affinity pheromone binding

Orientation dynamics of weakly Brownian particles in periodic viscous flows

Evolution equations for the orientation distribution of axisymmetric particles in periodic flows are derived in the regime of small but non-zero Brownian rotations. The equations are based on a multiple time scale approach that allows fast computation of the relaxation processes leading to statistical equilibrium. The approach has been applied to the calculation of the effective viscosity of a thin disk suspension in gravity waves.Comment: 16 pages, 7 eps figures include

Integrative Approaches to Environmental Sustainability at Universities: an overview of challenges and priorities

The principles of sustainable development are becoming extremely relevant for organisations. In the case of universities, these institutions can act as agents in promoting these principles within society. The literature contains a wide range of studies which show how universities may play a critical role in disseminating sustainability principles on the one hand, and their translation into practice, on the other. At present, many Higher Education Institutions are becoming more aware of their impact on the environment, and trying to understand the environmental needs and implications of their operations. Going further, some universities are incorporating sustainability principles into their activities. One of the questions that universities are now facing is how education for sustainable development can be translated into practice so that it can be effective in transforming society. This paper will discuss the need for and the usefulness of integrative approaches to implement sustainable development in higher education. In addition to a theoretical review of the state of the art, the paper will use case studies from the Hamburg University of Applied Sciences (Germany) and Bournemouth University (UK), to illustrate the effectiveness of integration of sustainable development principles in university research and teaching activities, and the many benefits integrative approaches may bring about

The scenario of two-dimensional instabilities of the cylinder wake under EHD forcing: A linear stability analysis

We propose to study the stability properties of an air flow wake forced by a dielectric barrier discharge (DBD) actuator, which is a type of electrohydrodynamic (EHD) actuator. These actuators add momentum to the flow around a cylinder in regions close to the wall and, in our case, are symmetrically disposed near the boundary layer separation point. Since the forcing frequencies, typical of DBD, are much higher than the natural shedding frequency of the flow, we will be considering the forcing actuation as stationary. In the first part, the flow around a circular cylinder modified by EHD actuators will be experimentally studied by means of particle image velocimetry (PIV). In the second part, the EHD actuators have been numerically implemented as a boundary condition on the cylinder surface. Using this boundary condition, the computationally obtained base flow is then compared with the experimental one in order to relate the control parameters from both methodologies. After validating the obtained agreement, we study the Hopf bifurcation that appears once the flow starts the vortex shedding through experimental and computational approaches. For the base flow derived from experimentally obtained snapshots, we monitor the evolution of the velocity amplitude oscillations. As to the computationally obtained base flow, its stability is analyzed by solving a global eigenvalue problem obtained from the linearized Navier–Stokes equations. Finally, the critical parameters obtained from both approaches are compared