The Fluctuating Pressure Field in a Supersonic Turbulent Boundary Layer

The fluctuating pressure field in a supersonic turbulent boundary laye

RFX2 is a candidate downstream amplifier of A-MYB regulation in mouse spermatogenesis

<p>Abstract</p> <p>Background</p> <p>Mammalian spermatogenesis involves formation of haploid cells from the male germline and then a complex morphological transformation to generate motile sperm. Focusing on meiotic prophase, some tissue-specific transcription factors are known (A-MYB) or suspected (RFX2) to play important roles in modulating gene expression in pachytene spermatocytes. The current work was initiated to identify both downstream and upstream regulatory connections for <it>Rfx2</it>.</p> <p>Results</p> <p>Searches of pachytene up-regulated genes identified high affinity RFX binding sites (X boxes) in promoter regions of several new genes: <it>Adam5</it>, <it>Pdcl2</it>, and <it>Spag6</it>. We confirmed a strong promoter-region X-box for <it>Alf</it>, a germ cell-specific variant of general transcription factor TFIIA. Using <it>Alf </it>as an example of a target gene, we showed that its promoter is stimulated by RFX2 in transfected cells and used ChIP analysis to show that the promoter is occupied by RFX2 in vivo. Turning to upstream regulation of the <it>Rfx2 </it>promoter, we identified a cluster of three binding sites (MBS) for the MYB family of transcription factors. Because testis is one of the few sites of <it>A-myb </it>expression, and because spermatogenesis arrests in pachytene in <it>A-myb </it>knockout mice, the MBS cluster implicates <it>Rfx2 </it>as an <it>A-myb </it>target. Electrophoretic gel-shift, ChIP, and co-transfection assays all support a role for these MYB sites in <it>Rfx2 </it>expression. Further, <it>Rfx2 </it>expression was virtually eliminated in <it>A-myb </it>knockout testes. Immunohistology on testis sections showed that A-MYB expression is up-regulated only after pachytene spermatocytes have clearly moved away from the tubule wall, which correlates with onset of RFX2 expression, whereas B-MYB expression, by contrast, is prevalent only in earlier spermatocytes and spermatogonia.</p> <p>Conclusion</p> <p>With an expanding list of likely target genes, RFX2 is potentially an important transcriptional regulator in pachytene spermatocytes. <it>Rfx2 </it>itself is a good candidate to be regulated by A-MYB, which is essential for meiotic progression. If <it>Alf </it>is a genuine RFX2 target, then <it>A-myb</it>, <it>Rfx2</it>, and <it>Alf </it>may form part of a transcriptional network that is vital for completion of meiosis and preparation for post-meiotic differentiation.</p

Evaluation of nonmetallic thermal protection materials for the manned space shuttle. Volume 1, task 1: Assessment of technical risks associated with utilization of nonmetallic thermal protection system

Technical problems of design and flight qualification of the proposed classes of surface insulation materials and leading edge materials were reviewed. A screening test plan, a preliminary design data test plan and a design data test plan were outlined. This program defined the apparent critical differences between the surface insulators and the leading edge materials, structuring specialized screening test plans for each of these two classes of materials. Unique testing techniques were shown to be important in evaluating the structural interaction aspects of the surface insulators and a separate task was defined to validate the test plan. In addition, a compilation was made of available information on proposed material (including metallic TPS), previous shuttle programs, pertinent test procedures, and other national programs of merit. This material was collected and summarized in an informally structured workbook

Switchable Adhesion of Soft Composites Induced by a Magnetic Field

Switchable adhesives have the potential to improve the manufacturing and recycling of parts and to enable new modes of motility for soft robots. Here, we demonstrate magnetically-switchable adhesion of a two-phase composite to non-magnetic objects. The composite's continuous phase is a silicone elastomer, and the dispersed phase is a magneto-rheological fluid. The composite is simple to prepare, and to mould to different shapes. When a magnetic field is applied, the magneto-rheological fluid develops a yield stress, which dramatically enhances the composite's adhesive properties. We demonstrate up to a nine-fold increase of the pull-off force of non-magnetic objects in the presence of a 250 mT field

Review of world experience and properties of materials for encapsulation of terrestrial photovoltaic arrays

Published and unpublished information relating to encapsulation systems and materials properties was collected by searching the literature and appropriate data bases (over 1,300 documents were selected and reviewed) and by personal contacts including site and company visits. A data tabulation summarizing world experience with terrestrial photovoltaic arrays (50 installations) is presented in the report. Based on criteria of properties, processability, availability, and cost, candidate materials were identified which have potential for use in encapsulation systems for arrays with a lifetime of over 20 years high reliability, an efficiency greater than 10 percent, a total price less than $500/kW, and a production capacity of 500,000 kW/yr. The recommended materials (all commercially available) include, depending upon the device design, various borosilicate and soda-lime glasses and numerous polymerics suitable for specific encapsulation system functions

Oxygen and hydrogen ion abundance in the near-Earth magnetosphere: Statistical results on the response to the geomagnetic and solar wind activity conditions

The composition of ions plays a crucial role for the fundamental plasma properties in the terrestrial magnetosphere. We investigate the oxygen-to-hydrogen ratio in the near-Earth magnetosphere from -10 RE<XGSE}< 10 RE. The results are based on seven years of ion flux measurements in the energy range ~10 keV to ~955 keV from the RAPID and CIS instruments on board the Cluster satellites. We find that (1) hydrogen ions at ~10 keV show only a slight correlation with the geomagnetic conditions and interplanetary magnetic field changes. They are best correlated with the solar wind dynamic pressure and density, which is an expected effect of the magnetospheric compression; (2) ~10 keV O+ ion intensities are more strongly affected during disturbed phase of a geomagnetic storm or substorm than >274 keV O+ ion intensities, relative to the corresponding hydrogen intensities; (3) In contrast to ~10 keV ions, the >274 keV O+ ions show the strongest acceleration during growth phase and not during the expansion phase itself. This suggests a connection between the energy input to the magnetosphere and the effective energization of energetic ions during growth phase; (4) The ratio between quiet and disturbed times for the intensities of ion ionospheric outflow is similar to those observed in the near-Earth magnetosphere at >274 keV. Therefore, the increase of the energetic ion intensity during disturbed time is more likely due to the intensification than to the more effective acceleration of the ionospheric source. In conclusion, the energization process in the near-Earth magnetosphere is mass dependent and it is more effective for the heavier ions

U/Pb zircon, strontium, and oxygen isotopic and geochronological study of the southernmost Sierra Nevada Batholith, California

The southernmost Sierra Nevada offers a view into the deep levels of the Mesozoic batholithic belt which constitutes much of the range to the north, and represents one of the major tectonic features of western North America. The main crystalline rocks of the study area are (1) the intrusive suite of Bear Valley, a middle Cretaceous tonalite batholith complex with coeval gabbroic intrusives, and (2) the gneiss complex of the Tehachapi Mountains, which consists of Early Cretaceous orthogneiss and subordinate paragneiss, with local domains having granulite facies metamorphic assemblages. The orthogneisses are dominantly tonalitic in composition, with significant layers of granodioritic to granitic and lesser dioritic to gabbroic gneiss. Quartz-rich and psammitic metasedimentary rocks with subordinate marble constitute the main framework assemblage into which the plutonic rocks were emplaced. Field relations demonstrate assimilation of metasedimentary material into the orthogneiss and tonalite batholith magmas, and magma mixing between mafic, tonalitic, and granitic materials. Significant domains of both homogenization and inhomogenization are recognized isotopically within the mixed rocks. U/Pb zircon studies have resolved two major igneous suites and a third suite of postdeformational intrusives, all lying between 90 and 120 Ma. The first suite (gneiss complex of the Tehachapi Mountains) was emplaced at ∼115 Ma, and exhibits penetrative high-temperature deformation developed at or near solidus conditions. A number of discordance patterns, along with the physical properties of the zircon, suggest minor inheritance of Proterozoic zircon and limited open system behavior in response to a major 100 Ma plutonic event. The 100 ± 3 Ma intrusive suite of Bear Valley crosscuts the older suite, but also exhibits significant synplutonic deformation. Mainly concordant zircon ages indicate the igneous crystallization age, but some discordances occur due to inheritance or entrainment of Proterozoic zircon. The high-temperature deformation fabrics in these suites and within the metasedimentary framework rocks were crosscut by the granodiorite of Claraville (90 Ma) and pegmatite dikes (∼95 Ma). The granodiorite of Claraville shows strong inheritance of Proterozoic zircon and high initial ^(87)Sr/^(86)Sr and δ^(18)O. Zircon populations from paragneiss and quartzite samples are dominated by Proterozoic detrital grains. Strontium and oxygen isotopic data on the zircon geochronology sample suite suggest simple twocomponent mixing of mantle-derived gabbroic to tonalitic magmas with partial to complete melt products from the metasedimentary framework rocks. Sedimentary admixtures for some granitic rocks may be as high as 45%, but for the tonalitic batholithic complex are no higher than about 15%. Modeled values of 10–20% metasediment are typical for the orthogneisses. Initial ^(87)Sr/^(86)Sr correlates directly with δ^(18)O, and generally correlates inversely with Sr content. Some subtle complexities in the Sr and O isotopic data suggest the involvement of a third cryptic component. Such a component could be early Phanerozoic ensimatic accretionary terranes that were structurally beneath the observed metasedimentary sequence, or altered oceanic crust and sediments introduced into the mantle magma source area by subduction. One of the initial aims of this study was to seek out remnants of Proterozoic sialic crystalline rocks within the gneiss complex of the Tehachapi Mountains. No such remnants were found, and our studies strongly suggest that sialic components within this link of the Mesozoic batholithic belt were introduced into mantle-derived magraatic systems by anatexis of continent-derived sedimentary rocks

Gamma-ray signatures of annihilation to charged leptons in dark matter substructure

Due to their higher concentrations and small internal velocities, Milky Way subhalos can be at least as important as the smooth halo in accounting for the GeV positron excess via dark matter annihilation. After showing how this can be achieved in various scenarios, including in Sommerfeld models, we demonstrate that, in this case, the diffuse inverse-Compton emission resulting from electrons and positrons produced in substructure leads to a nearly-isotropic signal close to the level of the isotropic GeV gamma-ray background seen by Fermi. Moreover, we show that HESS cosmic-ray electron measurements can be used to constrain multi-TeV internal bremsstrahlung gamma rays arising from annihilation to charged leptons.Comment: 8 pages, 4 figures; minor updates to match published versio

Methodology for designing accelerated aging tests for predicting life of photovoltaic arrays

A methodology for designing aging tests in which life prediction was paramount was developed. The methodology builds upon experience with regard to aging behavior in those material classes which are expected to be utilized as encapsulant elements, viz., glasses and polymers, and upon experience with the design of aging tests. The experiences were reviewed, and results are discussed in detail