63 research outputs found
Evaluating connectivity models for conservation: insights from African lion dispersal patterns
Context
Evaluating connectivity and identifying corridors for protection is a central challenge in applied ecology and conservation. Rigorous validation and comparison of how approaches perform in capturing biological processes is needed to guide research and conservation action.
Objectives
We aim to compare the ability of connectivity surfaces optimised using home range and dispersal data to accurately capture lion movement during dispersal, using cost-distance and circuit theory approaches.
Methods
We delineate periods of dispersal in African lions (Panthera leo) to obtain movement trajectories of dispersing individuals across the Kavango Zambezi Transfrontier Conservation Area, southern Africa. We use these trajectories to assess comparative measures of connectivity values at dispersal points across surfaces and the ability of models to discriminate between observed and randomised paths.
Results
Encouragingly, results show that on average, all connectivity approaches and resistance surfaces used perform well in predicting movements of an independent set of dispersing lions. Cost-distance approaches were generally more sensitive to resistance input than circuit theory, but differences in performance measures between resistance inputs were small across both approaches.
Conclusions
Findings suggest that home range data can be used to generate resistance surfaces for connectivity maps in this system, with independent dispersal data providing a promising approach to thresholding what is considered as “connected” when delineating corridors. Most dispersers traversed through landscapes that had minimal human settlement and are likely highly connected by dispersal. Research into limiting factors and dispersal abilities will be critical to understanding how populations will respond to increasing habitat fragmentation and human expansion
Collaboration for conservation: assessing countrywide carnivore occupancy dynamics from sparse data
Aim: Assessing the distribution and persistence of species across their range is a crucial component of wildlife conservation. It demands data at adequate spatial scales and over extended periods of time, which may only be obtained through collaborative efforts, and the development of methods that integrate heterogeneous datasets. We aimed to combine existing data on large carnivores to evaluate population dynamics and improve knowledge on their distribution nationwide.
Location: Botswana.
Methods: Between 2010 and 2016, we collated data on African wild dog, cheetah, leopard, brown and spotted hyaena and lion gathered with different survey methods by independent researchers across Botswana. We used a multi-species, multi-method dynamic occupancy model to analyse factors influencing occupancy, persistence and colonization, while accounting for imperfect detection. Lastly, we used the gained knowledge to predict the probability of occurrence of each species countrywide.
Results: Wildlife areas and communal rangelands had similar occupancy probabilities for most species. Large carnivore occupancy was low in commercial farming areas and where livestock density was high, except for brown hyaena. Lion occupancy was negatively associated with human density; lion and spotted hyaena occupancy was high where rainfall was high, while the opposite applied to brown hyaena. Lion and leopard occupancy remained constant countrywide over the study period. African wild dog and cheetah occupancy declined over time in the south and north, respectively, whereas both hyaena species expanded their ranges. Countrywide predictions identified the highest occupancy for leopards and lowest for the two hyaena species.
Main Conclusions: We highlight the necessity of data sharing and propose a generalizable analytical method that addresses the challenges of heterogeneous data common in ecology. Our approach, which enables a comprehensive multi-species assessment at large spatial and temporal scales, supports the development of data-driven conservation guidelines and the implementation of evidence-based management strategies nationally and internationally
The Cryptosporidium parvum Kinome
<p>Abstract</p> <p>Background</p> <p>Hundreds of millions of people are infected with cryptosporidiosis annually, with immunocompromised individuals suffering debilitating symptoms and children in socioeconomically challenged regions at risk of repeated infections. There is currently no effective drug available. In order to facilitate the pursuit of anti-cryptosporidiosis targets and compounds, our study spans the classification of the <it>Cryptosporidium parvum </it>kinome and the structural and biochemical characterization of representatives from the CDPK family and a MAP kinase.</p> <p>Results</p> <p>The <it>C</it>. <it>parvum </it>kinome comprises over 70 members, some of which may be promising drug targets. These <it>C. parvum </it>protein kinases include members in the AGC, Atypical, CaMK, CK1, CMGC, and TKL groups; however, almost 35% could only be classified as OPK (other protein kinases). In addition, about 25% of the kinases identified did not have any known orthologues outside of <it>Cryptosporidium spp</it>. Comparison of specific kinases with their <it>Plasmodium falciparum </it>and <it>Toxoplasma gondii </it>orthologues revealed some distinct characteristics within the <it>C. parvum </it>kinome, including potential targets and opportunities for drug design. Structural and biochemical analysis of 4 representatives of the CaMK group and a MAP kinase confirms features that may be exploited in inhibitor design. Indeed, screening <it>Cp</it>CDPK1 against a library of kinase inhibitors yielded a set of the pyrazolopyrimidine derivatives (PP1-derivatives) with IC<sub>50 </sub>values of < 10 nM. The binding of a PP1-derivative is further described by an inhibitor-bound crystal structure of <it>Cp</it>CDPK1. In addition, structural analysis of <it>Cp</it>CDPK4 identified an unprecedented Zn-finger within the CDPK kinase domain that may have implications for its regulation.</p> <p>Conclusions</p> <p>Identification and comparison of the <it>C. parvum </it>protein kinases against other parasitic kinases shows how orthologue- and family-based research can be used to facilitate characterization of promising drug targets and the search for new drugs.</p
Prevention of recrudescent malaria in nude mice by thymic grafting or by treatment with hyperimmune serum.
Nude mice died when infected with the normally avirulent malarial parasite Plasmodium berghei yoelii. Furthermore, malaria recrudesced in Nu/Nu mice after the termination of acute disease by treatment with clindamycin. Recrudescence was not observed in Nu/Nu mice that had been grafted with thymic tissue or treated with hyperimmune serum. Mice mad B cell deficient by treatment with anti-mu-chain serum also died when infected with P. berghei yoelii. The data suggest that a crucial role of the thymus in preventing recrudescent malaria in this model system is to provide a helper function in the production of protective antibody
Epstein--Barr virus-induced cell fusion
Serological and molecular biological studies have shown an association between Epstein--Barr virus (EBV) and nasopharyngeal carcinoma. Although it has been shown that the epithelioid tumour cells carry EBV genomes, they are apparently devoid of receptors for EBV (H.W., unpublished observations). Other have suggested that fusion of EBV carrying cells with epithelial cells may be the mode of entry of the virus into cells unable to absorb the virus and that this may be mediated by one of the known syncytium-forming viruses which inhabit the respiratory tract (for example, members of the paramyxovirus group). de Thé and colleagues suggested that intercellular bridges could be seen in NPC tumour material. We have developed a technique which permits the preparation of stable monolayers of viable human lymphoblastoid cell lines. Using this technique we have now demonstrated that EBV can induce fusion between EBV-superinfected lymphoblastoid cells and cells devoid of EBV receptors
A satellite perspective on the movement decisions of African elephants in relation to nomadic pastoralists
The African savannah ecosystem is populated by nomadic pastoralists who herd livestock in the day and corral them at night in temporary enclosures, called bomas, to protect them. The number and distribution of bomas on the savannah is important from an ecological perspective and may have a significant impact on wildlife movement. However, no study has yet examined this relationship. Here, using very high-resolution satellite imagery from two time periods, we quanitified changes in boma distribution and density across an area of 3377 km2 in the Laikipia-Samburu ecosystem of northern Kenya between 2011 and 2019. To assess wildlife movement in relation to bomas, we used a GPS data set on African bush elephant Loxodonta africana movement from 27 collared matriarchs representing herds of 9–15, covering 112 467 hourly GPS fixes over 31 months between 2018 and 2020. Our results showed a more than 46% increase in the total number of human-built structures between 2011 and 2019, the majority of which were bomas, representing a 21.9% increase in human-modified land area. Elephants readily adjusted their foraging habits and itineraries in this habitat shared with humans, who were also nomadic in space and time. Assessing the night–day activity ratio, we found elephants move more nocturnally when in closer proximity to bomas, particularly during the dry season. This temporal separation means elephants avoid the times humans are active in and around bomas while still accessing required resources—water and forage. The temporal shift was stronger during the dry season when shared resources are scarce. Using daily travel distance as a metric, we show elephants moved further in closer proximity to bomas which was likely linked to the need to travel between forage patches. Given the rise in human settlements, understanding the consequences of animals' behavioral adjustments is critical to understand the long-term population viability of elephant populations
Tumorh�ufigkeit nach R�ntgen-Ganzbestrahlung wei�er Ratten im Hunger- und im Durstzustand
Der Mechanismus der Blutkörperchensenkung V. Mitteilung: Auto-, Iso-, Homöo- und Heterosedimentation
- …