2,386 research outputs found
NORTHEAST SOYBEAN ACREAGE RESPONSE USING EXPECTED NET RETURNS
Expected prices and expected net returns from cropping activities are used to estimate soybean acreage response in the Northeast. Futures prices and lagged cash prices constitute proxies for price expectations. Expected net returns appear as good or better than expected prices for estimating acreage response. Short-run and long-run elasticities of soybean acreage with respect to expected net returns from soybeans are estimated as 0.5 and 1.6 for the northeast region. Soybean acreage appears less responsive to changes in expected net returns than to expected changes in prices.Crop Production/Industries,
Expert Finding by Capturing Organisational Knowledge from Legacy Documents
Organisations capitalise on their best knowledge through the improvement of shared expertise which leads to a higher level of productivity and competency. The recognition of the need to foster the sharing of expertise has led to the development of expert finder systems that hold pointers to experts who posses specific knowledge in organisations. This paper discusses an approach to locating an expert through the application of information retrieval and analysis processes to an organizationâs existing information resources, with specific reference to the engineering design domain. The approach taken was realised through an expert finder system framework. It enables the relationships of heterogeneous information sources with experts to be factored in modelling individualsâ expertise. These valuable relationships are typically ignored by existing expert finder systems, which only focus on how documents relate to their content. The developed framework also provides an architecture that can be easily adapted to different organisational environments. In addition, it also allows users to access the expertise recognition logic, giving them greater trust in the systems implemented using this framework. The framework were applied to real world application and evaluated within a major engineering company
Role of the Zn\u3csub\u3e1\u3c/sub\u3e and Zn\u3csub\u3e2\u3c/sub\u3e Sites in Metallo-β-lactamase L1
In an effort to probe the role of the Zn(II) sites in metallo-β-lactamase L1, mononuclear metal ion containing and heterobimetallic analogues of the enzyme were generated and characterized using kinetic and spectroscopic studies. Mononuclear Zn(II)-containing L1, which binds Zn(II) in the consensus Zn1 site, was shown to be slightly active; however, this enzyme did not stabilize a nitrocefin-derived reaction intermediate that had been previously detected. Mononuclear Co(II)- and Fe(III)-containing L1 were essentially inactive, and NMR and EPR studies suggest that these metal ions bind to the consensus Zn2 site in L1. Heterobimetallic analogues (ZnCo and ZnFe) analogues of L1 were generated, and stopped-flow kinetic studies revealed that these enzymes rapidly hydrolyze nitrocefin and that there are large amounts of the reaction intermediate formed during the reaction. The heterobimetallic analogues were reacted with nitrocefin, and the reactions were rapidly freeze quenched. EPR studies on these samples demonstrate that Co(II) is 5-coordinate in the resting state, proceeds through a 4-coordinate species during the reaction, and is 5-coordinate in the enzymeâproduct complex. These studies demonstrate that the metal ion in the Zn1 site is essential for catalysis in L1 and that the metal ion in the Zn2 site is crucial for stabilization of the nitrocefin-derived reaction intermediate
Conformational Changes in the Metallo-β-lactamase ImiS During the Catalytic Reaction: An EPR Spectrokinetic Study of Co(II)-Spin Label Interactions
Metallo-β-lactamases are responsible for conferring antibiotic resistance on certain pathogenic bacteria. In consequence, the search for inhibitors that may be useful in combating antibiotic resistance has fueled much study of the active sites of these enzymes. There exists circumstantial evidence that the binding of substrates and inhibitors to metallo-β-lactamases may involve binding to the organic part of the molecule, in addition to or prior to binding to one or more active site metal ions. It has also been postulated that a conformational change may accompany this putative binding. In the present study, electron paramagnetic resonance spectrokinetic study of a spin-labeled variant of the class B2 metallo-β-lactamase ImiS identified movement of a component residue on a conserved ι-helix in a catalytically competent time upon formation of a transient reaction intermediate with the substrate imipenem. In a significant subpopulation of ImiS, this conformational change was not associated with substrate binding to the active site metal ion but, rather, represents a distinct step in the reaction with ImiS. This observation has implications regarding the determinants of substrate specificity in metallo-β-lactamases and the design of potentially clinically useful inhibitors
\u3cem\u3eArabidopsis thaliana\u3c/em\u3e GLX2-1 Contains a Dinuclear Metal Binding Site, but Is Not a Glyoxalase 2
In an effort to probe the structure and function of a predicted mitochondrial glyoxalase 2, GLX2-1, from Arabidopsis thaliana, GLX2-1 was cloned, overexpressed, purified and characterized using metal analyses, kinetics, and UVâvisible, EPR, and 1H-NMR spectroscopies. The purified enzyme was purple and contained substoichiometric amounts of iron and zinc; however, metal-binding studies reveal that GLX2-1 can bind nearly two equivalents of either iron or zinc and that the most stable analogue of GLX2-1 is the iron-containing form. UVâvisible spectra of the purified enzyme suggest the presence of Fe(II) in the protein, but the Fe(II) can be oxidized over time or by the addition of metal ions to the protein. EPR spectra revealed the presence of an anti-ferromagnetically-coupled Fe(III)Fe(II) centre and the presence of a protein-bound high-spin Fe(III) centre, perhaps as part of a FeZn centre. No paramagnetically shifted peaks were observed in 1H-NMR spectra of the GLX2-1 analogues, suggesting low amounts of the paramagnetic, anti-ferromagnetically coupled centre. Steady-state kinetic studies with several thiolester substrates indicate that GLX2-1 is not a GLX2. In contrast with all of the other GLX2 proteins characterized, GLX2-1 contains an arginine in place of one of the metal-binding histidine residues at position 246. In order to evaluate further whether Arg246 binds metal, the R246L mutant was prepared. The metal binding results are very similar to those of native GLX2-1, suggesting that a different amino acid is recruited as a metal-binding ligand. These results demonstrate that Arabidopsis GLX2-1 is a novel member of the metallo-β-lactamase superfamily
Spectroscopic Studies on \u3cem\u3eArabidopsis\u3c/em\u3e ETHE1, a Glyoxalase II-like Protein
ETHE1 (ethylmalonic encephalopathy protein 1) is a β-lactamase fold-containing protein that is essential for the survival of a range of organisms. In spite of the apparent importance of this enzyme, very little is known about its function or biochemical properties. In this study Arabidopsis ETHE1 was over-expressed and purified and shown to bind tightly to 1.2 ¹ 0.2 equivalents of iron. 1H NMR and EPR studies demonstrate that the predominant oxidation state of Fe in ETHE1 is Fe(II), and NMR studies confirm that two histidines are bound to Fe(II). EPR studies show that there is no antiferromagnetically coupled Fe(III)Fe(II) center in ETHE1. Gel filtration studies reveal that ETHE1 is a dimer in solution, which is consistent with previous crystallographic studies. Although very similar in terms of amino acid sequence to glyoxalase II, ETHE1 exhibits no thioester hydrolase activity, and activity screening assays reveal that ETHE1 exhibits low level esterase activity. Taken together, ETHE1 is a novel, mononuclear Fe(II)-containing member of the β-lactamase fold superfamily
Impact reactivity of materials at very high oxygen pressure
The requirements for impact testing of materials in an oxygen atmosphere at pressures from 82.7 MPa (12,000 psi) to 172 MPa (25,000 psi) were evaluated. The impact tester system was evaluated for potential pressure increases from 69 MPa (10,000 psi) to 82.7 MPa (12,000 psi). The low pressure oxygen and nitrogen systems, the impact tower, the impact test cell, and the high pressure oxygen system were evaluated individually. Although the structural integrity of the impact test cell and the compressor were sufficient for operation at 82.7 MPa (12,000 psi), studies revealed possible material incompatibility at that pressure and above. It was recommended that if a component should be replaced for 82.7 MPa (12,000 psi) operation the replacement should meet the final objectives of 172 MPa (25,000 psi). Recommended changes in the system include; use of Monel 400 for pressures above 82.7 MPa (12,000 psi), use of bellows to replace the seal in the impact tester, use of a sapphire window attached to a fiber optic for event sensing, and use of a three diaphragm compressor
Geology of Northeastern Victoria Land, Antarctica
The northeastern corner of Victoria Land-an area of 50,000 square kilometers east of the Rennick Glacier and north of the Newnes Ice Shelf-was studied geologically during a helicopter-supported reconnaissance in 1964,extending and refining previous work by New Zealand geologists. The oldest rocks of the region are the plutonic rocks of the Freyberg Mountains and other ranges in the southwest part of the region. These schists, gneisses, migmatites, and granitic rocks are presumed to be correlative with the late Precambrian and early Paleozoic terrane of the Transantarctic Mountains. Lying east of these plutonic rocks are low-grade metamorphosed clastic sedimentary rocks presumed to be of middle Paleozoic age; the steep contact with the plutonic rocks appears to be a sheared unconformity. The basal (?) formation, polymict conglomerate and black slate, forms a northwest-trending outcrop belt 10km wide of steeply dipping rocks. Further northeast and presumably next higher stratigraphically is quartzite and quartz conglomerate, forming an outcrop belt 8-15km wide. The remaining metasedimentary rocks belong to the Robertson Bay Formation of metasiltstone and metagraywacke, tightly folded about northwest-trending axes and forming an outcrop belt 150km wide, from the Millen Range to Robertson Bay. Numerous stocks and small batholiths of hornblende-biotite granodiorite intrude and bake the Robertson Bay Formation. The continental Beacon Sandstone, intruded by thick dikes and sills of Jurassic (?) diabase, overlies the older rocks of the southwestern part of the region, and has been brocken by normal faults and folded gently. The Beacon is in turn overlain by plateau basalts. Late Cenozoic basalt-trachyte volcanism along the Ross Sea coast has formed large, north-trending Adare, Hallett, and Daniell Peninsulas and similar Coulman Island. Each of these masses consists largely of volcanic rocks extruded beneath ice sheets at least as thick as 1500m; the continental ice cap was much more extensive at times past than it is now. The volcanic rocks are chaotic palagonite breccias, flow breccias, pillow lavas, and complexes intrusive into them. Subaerial calderas, flows, and cones formed along the crestal rift zones. The pre-Tertiary rocks are upraised along faults at the edge of the Ross Sea. Peaks reaching altitudes of 4000m near the coast attest to the magnitude of late Cenozoic uplift
Motion of the Zinc Ions in Catalysis by a Dizinc Metallo-β-Lactamase
We report rapid-freeze-quench X-ray absorption spectroscopy of a dizinc metallo-β-lactamase (MβL) reaction intermediate. The Zn(II) ions in the dinuclear active site of the S. maltophilia Class B3 MβL move away from each other, by âź0.3 Ă
after 10 ms of reaction with nitrocefin, from 3.4 to 3.7 Ă
. Together with our previous characterization of the resting enzyme and its nitrocefin product complex, where the Zn(II) ion separation relaxes to 3.6 Ă
, these data indicate a scissoring motion of the active site that accompanies the ring-opening step. The average Zn(II) coordination number of 4.5 in the resting enzyme appears to be maintained throughout the reaction with nitrocefin. This is the first direct structural information available on early stage dizinc metallo-β-lactamase catalysis
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