Lebendzellaufnahmen mit einem aufrechten Lichtmikroskop zur Detektion von physiologischen oder tumorösen Zellvorgängen und deren Interaktion mit Bestrahlung und Medikamenten

Es handelt sich um eine experimentelle Arbeit zur Etablierung von kostengünstigen Lebendzellaufnahmen mit einem Lichtmikroskop. Verschiedene Versuchsreihen wurden dafür untersucht. Die Zellen der verschiedenen Versuchsreihen wurden mit Fluoreszenzfarbstoffen beimpft und es wurden Bildaufnahmen über einen Zeitraum von ca. 3-5 Tagen aufgenommen. Es wurden dabei drei Konditionen untersucht: Mitochondrien-, Nekrose- und Apoptoseaktivität. Ausgewertet konnte man schließlich die einzelnen Daten in diversen Diagrammen darstellen

Mental health in Diabetes Prevention and Intevention Programs in American Indian/Alaska Native Communities

American Indian and Alaska Natives youth and adults experience higher rates of type 2 diabetes and mental health problems than the general United States population. Few studies have explored the relationship other than detail the two issues independently. The present review aims to identify programs that seek to prevent/treat type 2 diabetes and mental health disorders in the American Indian and Alaska Native population. Available programs were reviewed for AI/AN adults and youth who suffer with both. As part of the review process, databases were searched for peer reviewed published studies. It was found that very few programs effectively incorporate mental health into the existing diabetes program. Four recommendations for future research are offered based on this literature review

Mental Health in Diabetes Prevention and Intervention Programs in American Indian/Alaska Native Communities

American Indian and Alaska Natives youth and adults experience higher rates of type 2 diabetes and mental health problems than the general United States population. Few studies have explored the relationship other than detail the two issues independently. The present review aims to identify programs that seek to prevent/treat type 2 diabetes and mental health disorders in the American Indian and Alaska Native population. Available programs were reviewed for AI/AN adults and youth who suffer with both. As part of the review process, databases were searched for peer reviewed published studies. It was found that very few programs effectively incorporate mental health into the existing diabetes program. Four recommendations for future research are offered based on this literature review

Stabilize to Act: Learning to Coordinate for Bimanual Manipulation

Key to rich, dexterous manipulation in the real world is the ability to coordinate control across two hands. However, while the promise afforded by bimanual robotic systems is immense, constructing control policies for dual arm autonomous systems brings inherent difficulties. One such difficulty is the high-dimensionality of the bimanual action space, which adds complexity to both model-based and data-driven methods. We counteract this challenge by drawing inspiration from humans to propose a novel role assignment framework: a stabilizing arm holds an object in place to simplify the environment while an acting arm executes the task. We instantiate this framework with BimanUal Dexterity from Stabilization (BUDS), which uses a learned restabilizing classifier to alternate between updating a learned stabilization position to keep the environment unchanged, and accomplishing the task with an acting policy learned from demonstrations. We evaluate BUDS on four bimanual tasks of varying complexities on real-world robots, such as zipping jackets and cutting vegetables. Given only 20 demonstrations, BUDS achieves 76.9% task success across our task suite, and generalizes to out-of-distribution objects within a class with a 52.7% success rate. BUDS is 56.0% more successful than an unstructured baseline that instead learns a BC stabilizing policy due to the precision required of these complex tasks. Supplementary material and videos can be found at https://sites.google.com/view/stabilizetoact .Comment: Conference on Robot Learning, 202

Invasion of the central nervous system by Cryptococcus neoformans requires a secreted fungal metalloprotease.

UnlabelledCryptococcus spp. cause life-threatening fungal infection of the central nervous system (CNS), predominantly in patients with a compromised immune system. Why Cryptococcus neoformans has this remarkable tropism for the CNS is not clear. Recent research on cerebral pathogenesis of C. neoformans revealed a predominantly transcellular migration of cryptococci across the brain endothelium; however, the identities of key fungal virulence factors that function specifically to invade the CNS remain unresolved. Here we found that a novel, secreted metalloprotease (Mpr1) that we identified in the extracellular proteome of C. neoformans (CnMpr1) is required for establishing fungal disease in the CNS. Mpr1 belongs to a poorly characterized M36 class of fungalysins that are expressed in only some fungal species. A strain of C. neoformans lacking the gene encoding Mpr1 (mpr1Δ) failed to breach the endothelium in an in vitro model of the human blood-brain barrier (BBB). A mammalian host infected with the mpr1Δ null strain demonstrated significant improvement in survival due to a reduced brain fungal burden and lacked the brain pathology commonly associated with cryptococcal disease. The in vivo studies further indicate that Mpr1 is not required for fungal dissemination and Mpr1 likely targets the brain endothelium specifically. Remarkably, the sole expression of CnMPR1 in Saccharomyces cerevisiae resulted in a robust migration of yeast cells across the brain endothelium, demonstrating Mpr1's specific activity in breaching the BBB and suggesting that Mpr1 may function independently of the hyaluronic acid-CD44 pathway. This distinct role for Mpr1 may develop into innovative treatment options and facilitate a brain-specific drug delivery platform.ImportanceCryptococcus neoformans is a medically relevant fungal pathogen causing significant morbidity and mortality, particularly in immunocompromised individuals. An intriguing feature is its strong neurotropism, and consequently the hallmark of cryptococcal disease is a brain infection, cryptococcal meningoencephalitis. For C. neoformans to penetrate the central nervous system (CNS), it first breaches the blood-brain barrier via a transcellular pathway; however, the identities of fungal factors required for this transmigration remain largely unknown. In an effort to identify extracellular fungal proteins that could mediate interactions with the brain endothelium, we undertook a proteomic analysis of the extracellular proteome and identified a secreted metalloprotease (Mpr1) belonging to the M36 class of fungalysins. Here we found that Mpr1 promotes migration of C. neoformans across the brain endothelium and into the CNS by facilitating attachment of cryptococci to the endothelium surface, thus underscoring the critical role of M36 proteases in fungal pathogenesis

Prospectus, January 25, 1989

https://spark.parkland.edu/prospectus_1989/1000/thumbnail.jp

An Intermediate Level of BMP Signaling Directly Specifies Cranial Neural Crest Progenitor Cells in Zebrafish

The specification of the neural crest progenitor cell (NCPC) population in the early vertebrate embryo requires an elaborate network of signaling pathways, one of which is the Bone Morphogenetic Protein (BMP) pathway. Based on alterations in neural crest gene expression in zebrafish BMP pathway component mutants, we previously proposed a model in which the gastrula BMP morphogen gradient establishes an intermediate level of BMP activity establishing the future NCPC domain. Here, we tested this model and show that an intermediate level of BMP signaling acts directly to specify the NCPC. We quantified the effects of reducing BMP signaling on the number of neural crest cells and show that neural crest cells are significantly increased when BMP signaling is reduced and that this increase is not due to an increase in cell proliferation. In contrast, when BMP signaling is eliminated, NCPC fail to be specified. We modulated BMP signaling levels in BMP pathway mutants with expanded or no NCPCs to demonstrate that an intermediate level of BMP signaling specifies the NCPC. We further investigated the ability of Smad5 to act in a graded fashion by injecting smad5 antisense morpholinos and show that increasing doses first expand the NCPCs and then cause a loss of NCPCs, consistent with Smad5 acting directly in neural crest progenitor specification. Using Western blot analysis, we show that P-Smad5 levels are dose-dependently reduced in smad5 morphants, consistent with an intermediate level of BMP signaling acting through Smad5 to specify the neural crest progenitors. Finally, we performed chimeric analysis to demonstrate for the first time that BMP signal reception is required directly by NCPCs for their specification. Together these results add substantial evidence to a model in which graded BMP signaling acts as a morphogen to pattern the ectoderm, with an intermediate level acting in neural crest specification

Reviving Traditional Native American Food with the Hunt. Fish. Gather. Program

In the United States, high incidences of cancer, cardiovascular disease, diabetes, depression, anxiety, and stress affect large portions of the population. One way to prevent, manage, and protect against these health-related difficulties is to maintain a healthy diet. To accomplish this, people can turn to traditional Native American foods, which are minimally processed, locally produced, nutrient-dense, and low in trans-fat. This study examined health awareness outcomes from the Hunt. Fish. Gather. (H.F.G.) program, a Native American cuisine program seeking to increase awareness of traditional Native foods with a focus on mind, body, spirit, and environment. Upon completion of the program, surveys were administered to participants to measure whether awareness and understanding of traditional Native foods changed. Findings of the survey suggest that the program significantly increased participant awareness and understanding of traditional Native foods. Literature suggests that increasing these may lead to better food habits and improved well-being

Prospectus, February 15, 1989

https://spark.parkland.edu/prospectus_1989/1002/thumbnail.jp