A structural comparison of the A and B subunits of Griffonia simplicifolia I isolectins

A structural comparison between the A and B subunits of the five tetrameric Griffonia simplicifolia I isolectins (A4, A3B, A2B2, AB3, B4) was undertaken to determine the extent of homology between the subunits. The first 25 N-terminal amino acids of both A and B subunits were determined following the enzymatic removal of N-terminal pyroglutamate blocking groups with pyroglutamate aminopeptidase. Although 21 amino acids were common to both subunits, there were four unique amino acids in the N-terminal sequence of A and B. Residues 8, 9, 17, and 19 were asparagine, leucine, lysine, and asparagine in subunit A and threonine, phenylalanine, glutamic acid, and serine in subunit B. The last six C-terminal amino acids, released by digestion with carboxypeptidase Y, were the same for both subunits: Arg---(Phe, Val)---Leu---Thr---Ser---COOH. Subunit B, which contains one methionyl residue, was cleaved by cyanogen bromide into two fragments, a large (Mr = 31,000) and a small (Mr = 2700) polypeptide. Failure of the small fragment to undergo manual Edman degradation indicated an N-terminal blocking group, presumably pyroglutamate. Both subunits were digested with trypsin and the tryptic peptides were analyzed using reverse-phase HPLC. Tryptic glycopeptides were identified by labeling the carbohydrate moiety of the A and B subunit using sodium [3H] borohydride. Cysteine-containing tryptic peptides were similarly identified by using [1-14C]iodoacetamide. Approximately 30% of the tryptic peptides were common to both subunits. Thus, although the N- and C-terminal regions of A and B are similar, the subunits each possess unique sequences.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/24903/1/0000330.pd

Att hantera sexualiteten i det tjugonde århundradet : en studie av sexualrådgivningen på ungdomsmottagningen och förmedlingen av normer från personal till ungdom

The subject of this paper is the mediation of norms about sexuality, from personnel working at the Swedish “ungdomsmottagning” (youth centre for service and counselling about sexual health), to the visiting teenagers. The purpose of the empirical study is to investigate how the staff understands the part of their work that implies giving advice about sexuality; how they work, which norms they have about youth sexuality and how they see their roll as advisers. To answer these questions, I have interviewed six staff members working at different youth centres. Three of them work as social counsellors and three as midwifes. The main area of work at the centre consists of individual appointments, but another important task is meeting with school classes visiting for purposes of study. Young people turn to midwifes to test themselves for sexually transmitted deceases, for counselling about contraceptives, etc. They turn to the social counsellors to talk about problems, relationships, their identity etc. As advisors the staff tries to act on how the teenagers themselves view their situation. It does occur though, that they express their own opinions and mediate norms. These norms are, for example, that the purpose with having sex should be to experience pleasure, that sex should be mutual and that young people should take responsibility for their sexuality. In this way, the workers influence how youth manage their sexuality. They play a part in young people’s socialisation. The motif of their actions is to foster the physical and mental health of the youth

Future possibilities for mobile control of electrical devices at home

Målen med denna undersökning har varit att studera vilka tekniker som idag finns för att möjliggöra mobil styrning av hemmet och därigenom underlätta elanvändarens roll i en uppgradering av det befintliga elnätet, vilken/vilka tekniker som bör få störst betydelse för framtidens smarta hem, samt hur en implementering av en mobil applikation för fjärrstyrning av hemmet skulle kunna tas fram. Undersökningen har i första hand genomförts genom en omfattande förstudie och informationsinhämtning, genom jämförelser av för- och nackdelar mellan olika nätverkstekniker, samt genom utredande av lämplig utvecklingsmiljö baserat på tillgång till öppet utvecklar API, produkttillgänglighet och support, samt initialkostnad för utvecklingsverktyg och system för testning. Undersökningen har visat att det idag inte finns någon klar vinnare bland nätverksteknikerna på området, även om Zigbee, Z-Wave och Wi-Fi är de starkaste kandidaterna till att vinna stora marknadsandelar. Det är således inte uppenbart att någon teknik kommer att få mycket större utbredning än de övriga då teknikerna istället kan komma att komplettera varandra då de är starka på olika områden. Undersökningen har också visat att som utvecklingsmiljö är det svenska företaget Telldus produktlösning (Tellstick) en relativt billig och enkel produkt att använda för att komma igång med styrning av hemmet, samt att de tillhandahåller två öppna APIer och uppmuntrar tredjepartsutveckling av applikationer för deras system.The objectives of this study has been to examine the techniques available for enabling home automation via mobile control (for instance via a smartphone) in order to facilitate the user's role in contributing to the upgrade of the present power-grid, but also to treat the matter of which of the techniques that will probably be of most importance for the future of the smart home, and how an implementation of a mobile application for remote home controlling could be developed. The investigation was primarily conducted through a comprehensive feasibility study of information gathering, comparison of the pros and cons of different networking techniques, and investigation of suitable development environments based on the availability of open developer APIs, availability of product solutions and support, and the cost of development tools for both development and system testing. The investigation has shown that there is currently no clear winner among the networking techniques in this area, although Zigbee, Z-Wave and Wi-Fi are the strongest candidates to gain large market shares. There is also no certainty that any technology will play a much greater role than the others since the techniques may complement each other because of their strength in different areas. The investigation also showed that as for a development environment the product solution developed by the Swedish company Telldus (called Tellstick) is relatively cheap and simple to use to get started with home automation, and that they encourage third party development by offering two open APIs for application development