Idealised simulations of cyclones with robust symmetrically-unstable sting jets

Idealised simulations of Shapiro-Keyser cyclones developing a sting jet (SJ) are presented. Thanks to an improved and accurate implementation of thermal wind balance in the initial state, it has been possible to use more realistic environments than in previous idealised studies. As a consequence, this study provides further insight in SJ evolution and dynamics and explores SJ robustness to different environmental conditions, assessed via a wide range of sensitivity experiments. The control simulation contains a cyclone that fits the Shapiro-Keyser conceptual model and develops a SJ whose dynamics are associated with the evolution of mesoscale instabilities along the airstream, including symmetric instability (SI). The SJ undergoes a strong descent while leaving the cloud-head banded tip and markedly accelerating towards the frontal-fracture region, revealed as an area of buckling of the already-sloped moist isentropes. Dry instabilities, generated by vorticity tilting via slantwise frontal motions in the cloud head, exist in similar proportions to moist instabilities at the start of the SJ descent and are then released along the SJ. The observed evolution supports the role of SI in the airstream’s dynamics proposed in a conceptual model outlined in a previous study. Sensitivity experiments illustrate that the SJ is a robust feature of intense Shapiro-Keyser cyclones, highlighting a range of different environmental conditions in which SI contributes to the evolution of this airstream, conditional on the model having adequate resolution. The results reveal that several environmental factors can modulate the strength of the SJ. However, a positive relationship between the strength of the SJ, both in terms of peak speed and amount of descent, and the amount of instability occurring along it can still be identified. In summary, the idealised simulations presented in this study show the robustness of SJ occurrence in intense Shapiro-Keyser cyclones and support and clarify the role of dry instabilities in SJ dynamics

Mechanisms producing different precipitation patterns over north‐eastern Italy: insights from HyMeX‐SOP1 and previous events

During the first HyMeX Special Observation Period (SOP1) field campaign, the target site of north‐eastern Italy (NEI) experienced a large amount of precipitation, locally exceeding the climatological values and distributed among several heavy‐rainfall episodes. In particular, two events that occurred during the last period of the campaign drew our attention. These events had common large‐scale patterns and a similar mesoscale setting, characterised by southerly low‐level flow interacting with the Alpine orography, but the precipitation distribution was very different. During Intensive Observing Period IOP18 (31 October–1 November 2012), convective systems were responsible for intense rainfall mainly located over a flat area of the eastern Po Valley, well upstream of the orography. Conversely, during IOP19 (4/5 November 2012), heavy precipitation affected only the Alpine area. In addition to IOP18 and IOP19, the present study analyses other heavy‐precipitation episodes that display similar characteristics and which occurred over NEI during the autumn of recent years. A high‐resolution (2 km grid spacing) non‐hydrostatic NWP model and available observations are used for this purpose. The two different observed precipitation patterns are explained in terms of interaction between the impinging flow and the Alps. Depending on the thermodynamic profile, convection can be triggered when the impinging flow is forced to rise over a pre‐existing cold‐air layer at the base of the orography. In this situation a persistent blocked‐flow condition and upstream convergence are responsible for heavy rain localized over the plain. Conversely, if convection does not develop, flow‐over conditions are established and heavy rain affects the Alps. Numerical parameters proposed in the literature are used to support the analysis. Finally, the role of evaporative cooling beneath the convective systems is evaluated. It turns out that the stationarity of the systems upstream of the Alps is mainly attributable to persistent blocked‐flow conditions, while convective outflow slightly modifies the location of precipitation

Elasto-viscoplastic modeling of subsidence above gas fields in the Adriatic Sea

Abstract. From the analysis of GPS monitoring data collected above gas fields in the Adriatic Sea, in a few cases subsidence responses have been observed not to directly correlate with the production trend. Such behavior, already described in the literature, may be due to several physical phenomena, ranging from simple delayed aquifer depletion to a much more complex time-dependent mechanical response of subsurface geomaterials to fluid withdrawal. In order to accurately reproduce it and therefore to be able to provide reliable forecasts, in the last years Eni has enriched its 3D finite element geomechanical modeling workflow by adopting an advanced constitutive model (Vermeer and Neher, 1999), which also considers the viscous component of the deformation. While the numerical implementation of such methodology has already been validated at laboratory scale and tested on synthetic hydrocarbon fields, the work herein presents its first application to a real gas field in the Adriatic Sea where the phenomenon has been observed. The results show that the model is capable to reproduce very accurately both GPS data and other available measurements. It is worth remarking that initial runs, characterized by the use of model parameter values directly obtained from the interpretation of mechanical laboratory tests, already provided very good results and only minor tuning operations have been required to perfect the model outcomes. Ongoing R&D projects are focused on a regional scale characterization of the Adriatic Sea basin in the framework of the Vermeer and Neher model approach

The role of mesoscale instabilities in the sting-jet dynamics of windstorm Tini

Sting jets (SJ) occur as an additional region of low-level strong winds in some Shapiro-Keyser-type extra-tropical cyclones. While SJs are widely accepted as being distinct from the warm and cold conveyor belts, the mechanisms responsible for their occurrence are still not fully understood. Here we determine the relative importance of the release of mesoscale instabilities and synoptic-scale cyclone dynamics, so addressing an area of current debate. Numerical weather prediction simulations of a SJ-containing windstorm are analysed and Lagrangian trajectories used to assess the evolution of, and mesoscale atmospheric instabilities (e.g. symmetric and inertial instabilities) in, the descending airstream. The SJ undergoes a two-stage descent: cooling via sublimation followed by a large acceleration accompanied by instability release. Combined tilting and stretching of vorticity play a major role in the local onset of instability on the airstream. Vorticity and frontogenesis fields have a narrow slantwise banded structure in the cloud head and around the SJ; the descending SJ modifies the widespread frontolysis expected from the large-scale dynamics alone in the frontal-fracture region. A coarser-resolution simulation also generates strong winds in the frontal-fracture region, although these are significantly weaker than in the higher-resolution simulation. The SJ airstream in the coarser-resolution simulation undergoes a weaker descent without instability generation and descends in a widespread frontolytic region. Hence, while the SJ undergoes a process of destabilisation that enhances its descent and acceleration in the higher-resolution simulation, enhancing the strong winds already generated by the synoptic-scale cyclone dynamics, this destabilisation does not occur in the SJ produced by a coarser-resolution simulation, resulting in weaker winds. This analysis reveals the synergy between the paradigms of SJ occurrence through the release of mesoscale instabilities and synoptic-scale cyclone dynamics and demonstrates that the current debate may in part be a consequence of the model resolutions used by different studies

Caveolin-3 Null Mice Show a Loss of Caveolae, Changes in the Microdomain Distribution of the Dystrophin-Glycoprotein Complex, and T-tubule Abnormalities

Caveolin-3, a muscle-specific caveolin-related protein, is the principal structural protein of caveolae membrane domains in striated muscle cells. Recently, we identified a novel autosomal dominant form of limb-girdle muscular dystrophy (LGMD-1C) in humans that is due to mutations within the coding sequence of the human caveolin-3 gene (3p25). These LGMD-1C mutations lead to an approximately 95% reduction in caveolin-3 protein expression, i.e. a caveolin-3 deficiency. Here, we created a caveolin-3 null (CAV3 -/-) mouse model, using standard homologous recombination techniques, to mimic a caveolin-3 deficiency. We show that these mice lack caveolin-3 protein expression and sarcolemmal caveolae membranes. In addition, analysis of skeletal muscle tissue from these caveolin-3 null mice reveals: (i) mild myopathic changes; (ii) an exclusion of the dystrophin-glycoprotein complex from lipid raft domains; and (iii) abnormalities in the organization of the T-tubule system, with dilated and longitudinally oriented T-tubules. These results have clear mechanistic implications for understanding the pathogenesis of LGMD-1C at a molecular level

The role of boundary layer processes in summer-time Arctic cyclones

Arctic cyclones are the most energetic weather systems in the Arctic, producing strong winds and precipitation that present major weather hazards. In summer, when the sea ice cover is reduced and more mobile, Arctic cyclones can have large impacts on ocean waves and sea ice. While the development of mid-latitude cyclones is known to be dependent on boundary layer (BL) turbulent fluxes, the dynamics of summer-time Arctic cyclones and their dependence on surface exchange processes have not been investigated. The purpose of this study is to characterise the BL processes acting in summer-time Arctic cyclones and understand their influence on cyclone evolution. The study focuses on two cyclone case studies, each characterised by a different structure during growth in the Arctic: (A) low-level-dominant vorticity (warm-core) structure and (B) upper-level-dominant vorticity (cold-core) structure, linked with a tropopause polar vortex. A potential vorticity (PV) framework is used to diagnose the BL processes in model runs from the ECMWF Integrated Forecasting System model. Both cyclones are associated with frictional Ekman pumping and downward sensible heat fluxes over sea ice. However, a third process, the frictional baroclinic generation of PV, acts differently in A and B due to differences in their low-level temperature structures. Positive PV is generated in Cyclone A near the bent-back warm front, like in typical mid-latitude cyclones. However, the same process produces negative PV tendencies in B, shown to be a consequence of the vertically aligned axisymmetric cold-core structure. This frictional process also acts to cool the lower troposphere, reducing the warm-core anomaly in A and amplifying the cold-core anomaly in B. Both cyclones attain a vertically aligned cold-core structure that persists for several days after maximum intensity, which is consistent with cooling from frictional Ekman pumping, frictional baroclinic PV generation, and downward sensible heat fluxes. This may help to explain the longevity of isolated cold-core Arctic cyclones with columnar vorticity structure.</p

Mutated CaV2.1 channels dysregulate CASK/P2X3 signaling in mouse trigeminal sensory neurons of R192Q Cacna1a knock-in mice

Background: ATP-gated P2X3 receptors of sensory ganglion neurons are important transducers of pain as they adapt their expression and function in response to acute and chronic nociceptive signals. The present study investigated the role of calcium/calmodulin-dependent serine protein kinase (CASK) in controlling P2X3 receptor expression and function in trigeminal ganglia from Cacna1a R192Q-mutated knock-in (KI) mice, a genetic model for familial hemiplegic migraine type-1.Results: KI ganglion neurons showed more abundant CASK/P2X3 receptor complex at membrane level, a result that likely originated from gain-of-function effects of R192Q-mutated CaV2.1 channels and downstream enhanced CaMKII activity. The selective CaV2.1 channel blocker \u3c9-Agatoxin IVA and the CaMKII inhibitor KN-93 were sufficient to return CASK/P2X3 co-expression to WT levels. After CASK silencing, P2X3 receptor expression was decreased in both WT and KI ganglia, supporting the role of CASK in P2X3 receptor stabilization. This process was functionally observed as reduced P2X3 receptor currents.Conclusions: We propose that, in trigeminal sensory neurons, the CASK/P2X3 complex has a dynamic nature depending on intracellular calcium and related signaling, that are enhanced in a transgenic mouse model of genetic hemiplegic migraine. \ua9 2013 Gnanasekaran et al.; licensee BioMed Central Ltd

Serum amyloid A primes microglia for ATP-dependent interleukin-1\u3b2 release

Acute-phase response is a systemic reaction to environmental/inflammatory insults and involves production of acute-phase proteins, including serum amyloid A (SAA). Interleukin-1\u3b2 (IL-1\u3b2), a master regulator of neuroinflammation produced by activated inflammatory cells of the myeloid lineage, in particular microglia, plays a key role in the pathogenesis of acute and chronic diseases of the peripheral nervous system and CNS. IL-1\u3b2 release is promoted by ATP acting at the purinergic P2X7 receptor (P2X7R) in cells primed with toll-like receptor (TLR) ligands

The steroid-hormone ecdysone coordinates parallel pupariation neuromotor and morphogenetic subprograms via epidermis-to-neuron Dilp8-Lgr3 signal induction

Funding Information: We thank Drs. Carlos Ribeiro, Christen Mirth, Elio Sucena, Filip Port, Frank Schnorrer, Julien Colombani, Maria Dominguez, Maria Luisa Vasconcelos, Pierre Leopold, Simon Bullock, Rita Teodoro, Gerald Rubin, Melissa Harrison, Kate O’Connor-Giles, Jill Wildonger, Mariana Melani, Pablo Wappner, and Christian Wegener for fly stocks and reagents. We thank Ryohei Yagi and Konrad Basler for the LHV2 plasmid and Brain McCabe for the mhc-Gateway destination plasmid. We thank Carlos Ribeiro and Dennis Goldschmidt for help in designing and constructing one of the pupariation arenas and Mariana Melani, Pablo Wappner, Arash Bashirullah, and Filip Port for sharing resources and unpublished data. We thank Arash Bashirullah, Fillip Port, and Carlos Ribeiro for discussions and/or comments on the manuscript, and Jim Truman for discussions on Fraenkel’s pupariation factors. Stocks obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537) were used in this study. Work in the Integrative Biomedicine Laboratory was supported by the European Commission FP7 (PCIG13-GA-2013-618847), by the FCT (IF/00022/2012; Congento LISBOA-01-0145-FEDER-022170, cofinanced by FCT/Lisboa2020; UID/Multi/04462/2019; PTDC/BEXBCM/1370/2014; PTDC/MED-NEU/30753/2017; PTDC/BIA-BID/31071/2017; FCT SFRH/BPD/94112/ 2013; SFRH/BD/94931/2013), the MIT Portugal Program (MIT-EXPL/BIO/0097/2017), and FAPESP (16/09659-3, 16/10342-4, and 17/17904-0). AG is a CONICET researcher, YV holds a CONICET postdoctoral fellowship and FPS and MJD hold a PhD fellowship from CONICET. Work in the Garelli lab was supported by ANPCyT (Agencia Nacional para la Promoción de la Ciencia y la Tecnología, PICT 2014-2900 and PICT 2017-0254) and CONICET (PIP11220150100182CO). Publisher Copyright: © 2021, The Author(s). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.Innate behaviors consist of a succession of genetically-hardwired motor and physiological subprograms that can be coupled to drastic morphogenetic changes. How these integrative responses are orchestrated is not completely understood. Here, we provide insight into these mechanisms by studying pupariation, a multi-step innate behavior of Drosophila larvae that is critical for survival during metamorphosis. We find that the steroid-hormone ecdysone triggers parallel pupariation neuromotor and morphogenetic subprograms, which include the induction of the relaxin-peptide hormone, Dilp8, in the epidermis. Dilp8 acts on six Lgr3-positive thoracic interneurons to couple both subprograms in time and to instruct neuromotor subprogram switching during behavior. Our work reveals that interorgan feedback gates progression between subunits of an innate behavior and points to an ancestral neuromodulatory function of relaxin signaling.publishersversionpublishe