Bone sialoprotein is predictive of bone metastases in resectable non small cell lung carcinoma: A case–control study and prevalence data

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Cytology of Primary Salivary Gland-Type Tumors of the Lower Respiratory Tract: Report of 15 Cases and Review of the Literature.

Primary pulmonary salivary gland-type tumors are rare neoplasms arising from the seromucinous submucosal glands of the lower respiratory tract (LRT), the most common of which are mucoepidermoid carcinoma (MEC) and adenoid cystic carcinoma. They are morphologically indistinguishable from their salivary gland counterpart and recognizing them is a challenge, especially on cytological specimens. We analyzed 15 cases of histologically proven primary salivary gland tumors of the LRT to identify cytomorphological features and define potential diagnostic clues that might assist cytopathologists in the preoperative diagnosis of these neoplasias. Three out of the four cases of adenoid cystic carcinomas showed the characteristic tridimensional cell clusters and hyaline globules, whereas the last one did not show malignant cells; only two cases of MEC presented the three characteristic cell types (i.e., squamous, intermediate, and mucin secreting) on cytology. Since these neoplasms are rare and do not have a completely specific set of cytological features, it is important for practicing cytopathologists to be aware of the possibility of encountering them, in specimens from patients with LRT masses, in order to render the correct diagnosis

Pulmonary responses to silica dust : the influence of crystalline structures and physico-chemical properties

The inhalation of silica crystalline polymorphs leads to the development of pulmonary nodules that vary in extent and in fibrotic content. In this study, a single protocol was used to test different silica samples (i.e., 2 non-crystalline samples: one vitreous silica, one diatomaceous earth , 4 crystalline samples: one pure a-quartz ground in a wet atmosphere, one of the same quartz heated until it becomes oc-cristobalite and 2 cristobalites obtained by heating diatomaceous earth). Wistar rats received a single intra-tracheal injection of alveolar dust and were followed over a period of 3 months. The results were exploited using macroscopic examination (wet lung weight), histological examination and biochemical dosing (hydroxyproline, lipid, and silica content). The results showed that vitreous silica, diatomaceous earth, wet-ground quartz and both types of cristobalites are responsible to a greater or lesser extent for the pulmonary reaction typical of exposure to silica (i.e., nodules ); with different cellular and fibrotic surface areas for each sample. a-cristobalite (obtained from the wet-ground quartz heated to a very high temperature; 1,300 °C) leads to the formation of only small foreign-body granulomas. All crystalline minerals tested caused alveolar lipoproteinosis. It was thus concluded that the crystalline structure plays a less important role than the surface state in the formation of silicotic nodules

Особливості функціонування оцінних дієслівних лексем у публіцистичному дискурсі

Стаття присвячена дослідженню семантико-стилістичних особливостей оціночної дієслівної лексики в публіцистичному дискурсі, виділенню тематичних груп інгерентно та адгерентно оціночної дієслівної лексики, а також визначенню основних типів сенсибилізації оціночних дієслів-прагмем. Ключові слова: публіцистичний дискурс, дискурс, прагмема, іформема, дієслова інгерентної оцінки, адгерентно оціночні дієслова, дієслівна прагмема, сенсибілізація, лінгвістичні сенсибілізатори.Статья посвящена исследованию семантико-стилистических особенностей оценочной глагольной лексики в публицистическом дискурсе, выделению тематических групп ингерентно и адгерентно оценочной глагольной лексики, а также определению основных типов сенсибилизации оценочных глаголов-прагмем. Ключевые слова: публицистический дискурс, прагмема, информема, глаголы ингерентной оценки, адгерентно оценочные глаголы, глагольная прагмема, сенсибилизация, лингвистические сенсибилизаторы.The article is dedicated to study of the semantic-stylistic features of estimated verbal vocabulary in publicistic discourse, to allocation of thematic groups ingerentno and angerentno estimated verbal vocabulary, and also to definition of the basic types of a sensubulisation of estimated verbs-pragmem. Keywords: Publicistic discourse, pragmems, informem, the ingerentno estimated verbs, the angerentno estimated verbs, verbal pragmems, a sensubulisation, linguistic sensubulisator

BRCA1- A ssociated protein 1 (BAP1) immunohistochemical expression as a diagnostic tool in malignant pleural mesothelioma classification: A large retrospective study

Malignant pleural mesothelioma (MPM) is a highly aggressive disease with limited therapeutic options. Histological subtype remains among the most reliable prognostic factors, because the epithelioid subtype associated with the best prognosis and the sarcomatoid subtype with the worst. The biphasic subtype has an intermediate prognosis, but its definitive histological diagnosis may be challenging owing to the difficulty of assessing the neoplastic nature of the stromal component. Recent data identified BRCA1-associated protein 1 gene (BAP1) as one of the most frequently mutated genes in MPM. Immunohistochemical testing for BRCA1-associated protein 1 (BAP1) has been proposed to be predictive for the detection of BAP1 mutation in neoplastic cells. The aim of the present study was to define the diagnostic usefulness of immunohistochemical determination of BAP1 in MPM, with clinicopathological correlation

Micro-RNA-215 and -375 regulate thymidylate synthase protein expression in pleural mesothelioma and mediate epithelial to mesenchymal transition

The standard front-line treatment for pleural mesothelioma (PM) is pemetrexed-based chemotherapy, whose major target is thymidylate synthase (TS). In several cancer models, miR-215 and miR-375 have been shown to target TS, while information on these miRNAs in PM are still limited although suggest their role in epithelial to mesenchymal transition. Seventy-one consecutive PM tissues (4 biphasic, 7 sarcomatoid, and 60 epithelioid types) and 16 commercial and patient-derived PM cell lines were screened for TS, miR-215, and miR-375 expression. REN and 570B cells were selected for miR-215 and miR-375 transient transfections to test TS modulation. ZEB1 protein expression in tumor samples was also tested. Moreover, genetic profile was investigated by means of BAP1 and p53 immunohistochemistry. Expression of both miR-215 and miR-375 was significantly higher in epithelioid histotype. Furthermore, inverse correlation between TS protein and both miR-215 and miR-375 expression was found. Efficiently transfected REN and 570B cell lines overexpressing miR-215 and miR-375 showed decreased TS protein levels. Epithelioid PM with a mesenchymal component highlighted by reticulin stain showed significantly higher TS and ZEB1 protein and lower miRNA expression. A better survival was recorded for BAP1 lost/TS low cases. Our data indicate that miR-215 and miR-375 are involved in TS regulation as well as in epithelial-to-mesenchymal transition in PM. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00428-022-03321-8