247 research outputs found
Gaia view of low-mass star formation
Understanding how young stars and their circumstellar disks form and evolve
is key to explain how planets form. The evolution of the star and the disk is
regulated by different processes, both internal to the system or related to
their environment. The former include accretion of material onto the central
star, wind emission, and photoevaporation of the disk due to high-energy
radiation from the central star. These are best studied spectroscopically, and
the distance to the star is a key parameter in all these studies. Here we
present new estimates of the distance to a complex of nearby star-forming
clouds obtained combining TGAS distances with measurement of extinction on the
line of sight. Furthermore, we show how we plan to study the effects of the
environment on the evolution of disks with Gaia, using a kinematic modelling
code we have developed to model young star-forming regions.Comment: 4 pages, 1 figure. To appear in the Proceedings of IAU Symposium 330:
Astrometry and Astrophysics in the Gaia Sk
Cationic siRNAs Provide Carrier-Free Gene Silencing in Animal Cells
siRNA-mediated gene silencing requires intracellular delivery of the nucleic acid. We have developed a carrierless molecular approach that follows the same cell entry route as cationic supramolecular complexes, yet should avoid the extracellular barriers encountered by nanoparticles. Cationic oligospermine−oligonucleotide conjugates (ZNAs, for Zip Nucleic Acids) were synthesized stepwise on an oligonucleotide synthesizer using a DMT-spermine phosphoramidite derivative. They were shown to enter cells and have access to the cytoplasm, provided their formal charge ratio N/P was >1.5. Cationic siRNAs that fulfilled this condition were shown to achieve selective inhibition of luciferase gene expression in the submicromolar concentration range in constitutively luciferase-expressing cells
Simulation of an SEIR infectious disease model on the dynamic contact network of conference attendees
The spread of infectious diseases crucially depends on the pattern of
contacts among individuals. Knowledge of these patterns is thus essential to
inform models and computational efforts. Few empirical studies are however
available that provide estimates of the number and duration of contacts among
social groups. Moreover, their space and time resolution are limited, so that
data is not explicit at the person-to-person level, and the dynamical aspect of
the contacts is disregarded. Here, we want to assess the role of data-driven
dynamic contact patterns among individuals, and in particular of their temporal
aspects, in shaping the spread of a simulated epidemic in the population.
We consider high resolution data of face-to-face interactions between the
attendees of a conference, obtained from the deployment of an infrastructure
based on Radio Frequency Identification (RFID) devices that assess mutual
face-to-face proximity. The spread of epidemics along these interactions is
simulated through an SEIR model, using both the dynamical network of contacts
defined by the collected data, and two aggregated versions of such network, in
order to assess the role of the data temporal aspects.
We show that, on the timescales considered, an aggregated network taking into
account the daily duration of contacts is a good approximation to the full
resolution network, whereas a homogeneous representation which retains only the
topology of the contact network fails in reproducing the size of the epidemic.
These results have important implications in understanding the level of
detail needed to correctly inform computational models for the study and
management of real epidemics
Lab-on-a-chip for multiplexed biosensing of residual antibiotics in milk
A multiplexed immunoassay-based antibiotic sensing device integrated in a lab-on-a-chip format is described. The approach is multidisciplinary and involves the convergent development of a multi-antibiotic competitive immunoassay based on sensitive wavelength interrogated optical sensor (WIOS) technology and a polymer-based self-contained microfluidic cartridge. Immunoassay solutions are pressure-driven through external and concerted actuation of a single syringe pump and multiposition valve. Moreover, the use of a novel photosensitive material in a one step fabrication process allowed the rapid fabrication of microfluidic components and interconnection port simultaneously. Pre-filled microfluidic cartridges were used as binary response rapid tests for the simultaneous detection of three antibiotic families – sulfonamides, fluoroquinolones and tetracyclines – in raw milk. For test interpretation, any signal lower than the threshold value obtained for the corresponding Maximum Residue Limit (MRL) concentration (100 µg L-1) was considered negative for a given antibiotic. The reliability of the multiplexed detection system was assessed by way of a validation test carried out on a series of six blind milk samples. A test accuracy of 95% was calculated from this experiment. The whole immunoassay procedure is fast (less than 10 minutes) and easy to handle (automated actuation)
High-resolution measurements of face-to-face contact patterns in a primary school
Little quantitative information is available on the mixing patterns of
children in school environments. Describing and understanding contacts between
children at school would help quantify the transmission opportunities of
respiratory infections and identify situations within schools where the risk of
transmission is higher. We report on measurements carried out in a French
school (6-12 years children), where we collected data on the time-resolved
face-to-face proximity of children and teachers using a proximity-sensing
infrastructure based on radio frequency identification devices.
Data on face-to-face interactions were collected on October 1st and 2nd,
2009. We recorded 77,602 contact events between 242 individuals. Each child has
on average 323 contacts per day with 47 other children, leading to an average
daily interaction time of 176 minutes. Most contacts are brief, but long
contacts are also observed. Contacts occur mostly within each class, and each
child spends on average three times more time in contact with classmates than
with children of other classes. We describe the temporal evolution of the
contact network and the trajectories followed by the children in the school,
which constrain the contact patterns. We determine an exposure matrix aimed at
informing mathematical models. This matrix exhibits a class and age structure
which is very different from the homogeneous mixing hypothesis.
The observed properties of the contact patterns between school children are
relevant for modeling the propagation of diseases and for evaluating control
measures. We discuss public health implications related to the management of
schools in case of epidemics and pandemics. Our results can help define a
prioritization of control measures based on preventive measures, case
isolation, classes and school closures, that could reduce the disruption to
education during epidemics
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