Glucose metabolism in different regions of the rat brain under hypokinetic stress influence

Glucose metabolism in rats kept under long term hypokinetic stress was studied in 7 brain regions. Determination was made of the regional levels of glucose, lactate, glutamate, glutamine, aspartate, gamma-aminobutyrate and the incorporation of C-14 from plasma glucose into these metabolites, in glycogen and protein. From the content and activity data the regional glucose flux was approximated quantitatively. Under normal conditions the activity gradient cortex and frontal pole cerebellum, thalamus and mesencephalon, hypothalamus and pons and medulla is identical with that of the regional blood supply (measured with I131 serum albumin as the blood marker). Within the first days of immobilization a functional hypoxia occurred in all brain regions and the utilization of cycle amino acids for protein synthesis was strongly diminished. After the first week of stress the capillary volumes of all regions increased, aerobic glucose metabolism was enhanced (factors 1.3 - 2.0) and the incorporation of glucose C-14 via cycle amino acids into protein was considerably potentiated. The metabolic parameters normalized between the 7th and 11th week of stress. Blood supply and metabolic rate increased most in the hypothalamus

Quasi-hydrostatic intracluster gas under radiative cooling

Quasi-hydrostatic cooling of the intracluster gas is studied. In the quasi-hydrostatic model, work done by gravity on the inflow gas with dP \neq 0, where P is the gas pressure, is taken into account in the thermal balance. The gas flows in from the outer part so as to compensate the pressure loss of the gas undergoing radiative cooling, but the mass flow is so moderate and smooth that the gas is considered to be quasi-hydrostatic. The temperature of the cooling gas decreases toward the cluster center, but, unlike cooling flows with dP = 0, approaches a constant temperature of \sim 1/3 the temperature of the non-cooling ambient gas. This does not mean that gravitational work cancels out radiative cooling, but means that the temperature of the cooling gas appears to approach a constant value toward the cluster center if the gas maintains the quasi-hydrostatic balance. We discuss the mass flow in quasi-hydrostatic cooling, and compare it with the standard isobaric cooling flow model. We also discuss the implication of \dot{M} for the standard cooling flow model.Comment: 5 pages, 1 figure, accepted for publication in A&

Calcitonin receptor-like receptor is expressed on gastrointestinal immune cells

Background/Aims: Pharmacological and morphological studies suggest that the gut mucosal immune system and local neuropeptide-containing neurones interact. We aimed to determine whether gut immune cells are targets for calcitonin gene-related peptide (CGRP), which has potent immune regulatory properties. Methods: Using density gradient centrifugation, rat lamina propria mononuclear cells (LP-MNCs) and intra-epithelial lymphocytes (IELs) were isolated. RT-PCR was employed for the detection of mRNA of rat calcitonin receptor-like receptor (CRLR), which is considered to represent the pharmacologically defined CGRP receptor-1 subtype, as well as mRNA of the receptor activity-modifying proteins, which are essential for CRLR function and determine ligand specificity. A radioreceptor assay was employed for the detection of specific CGRP binding sites. Results: RT-PCR and DNA sequencing showed that LP-MNCs and IELs express CRLR. Incubation of isolated LP-MNCs with radiolabelled alphaCGRP revealed the existence of specific binding sites for CGRP. Conclusion: These novel data indicate that mucosal immune cells of the rat gut are a target for CGRP and provide significant evidence that CGRP functions as an immune regulator in the gut mucosa. Copyright (C) 2002 S. Karger AG, Basel

The Isoelectric Focusing of Creatine Kinase Variants: I. The Heterogeneity of Creatine Kinase in Human Heart Cytosol and Mitochondria

Peer Reviewe

Metabolic costs of bat echolocation in a non-foraging context support a role in communication

The exploitation of information is a key adaptive behavior of social animals, and many animals produce costly signals to communicate with conspecifics. In contrast, bats produce ultrasound for auto-communication, i.e., they emit ultrasound calls and behave in response to the received echo. However, ultrasound echolocation calls produced by non-flying bats looking for food are energetically costly. Thus, if they are produced in a non-foraging or navigational context this indicates an energetic investment, which must be motivated by something. We quantified the costs of the production of such calls, in stationary, non-foraging lesser bulldog bats (Noctilio albiventris) and found metabolic rates to increase by 0.021 ± 0.001 J/pulse (mean ± standard error). From this, we estimated the metabolic rates of N. albiventris when responding with ultrasound echolocation calls to playbacks of echolocation calls from familiar and unfamiliar conspecific as well as heterospecific bats. Lesser bulldog bats adjusted their energetic investment to the social information contained in the presented playback. Our results are consistent with the hypothesis that in addition to orientation and foraging, ultrasound calls in bats may also have function for active communication

Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota

Engineering commensal organisms for challenging applications, such as modulating the gut ecosystem, is hampered by the lack of genetic parts. Here, we describe promoters, ribosome-binding sites, and inducible systems for use in the commensal bacterium Bacteroides thetaiotaomicron, a prevalent and stable resident of the human gut. We achieve up to 10,000-fold range in constitutive gene expression and 100-fold regulation of gene expression with inducible promoters and use these parts to record DNA-encoded memory in the genome. We use CRISPR interference (CRISPRi) for regulated knockdown of recombinant and endogenous gene expression to alter the metabolic capacity of B. thetaiotaomicron and its resistance to antimicrobial peptides. Finally, we show that inducible CRISPRi and recombinase systems can function in B. thetaiotaomicron colonizing the mouse gut. These results provide a blueprint for engineering new chassis and a resource to engineer Bacteroides for surveillance of or therapeutic delivery to the gut microbiome.National Science Foundation (U.S.) (Grant EEC-0540879)National Institutes of Health (U.S.) (Grants P50GM098792, 1DP2OD008435, 1R01EB017755, and GM095765)United States. Defense Advanced Research Projects Agency (Grant CLIO N66001-12-C-4016)United States. Defense Threat Reduction Agency (Grant HDTRA1-14-1-0007)United States. Office of Naval Research (Grant N00014-13-1-0424)Massachusetts Institute of Technology. Center for Microbiome Informatics and TherapeuticsQUALCOMM Inc. (Innovation Fellowship