The crisis and employment in Italy

The fall in employment and the increase in unemployment rates in Italy in 2009 were fairly modest, given the sharp drop in GDP and compared with the recession of the early 1990s. This work shows that these data should be interpreted with caution, however. Firstly, employment trends as measured by Italian labour force survey may understate the decline in total employment if, as seems plausible, a lag exists between the entry of immigrants into the country and their registration. Secondly, the rise in the unemployment rate has been curbed by extensive recourse to temporary income support schemes to reduce working hours (such as the Cassa integrazione guadagni or Wage Supplementation Fund) in the northern regions, and by the sharp drop in participation in the South (the discouragement effect). The results of the Bank of Italy’s Survey of Industrial and Service Firms conducted in September 2009 show that the largest employment cuts occurred in the firms most exposed to international markets. Based on estimated labour input elasticity and on the available GDP forecast for 2010-11, we calculate that Italian employment is likely to remain well below its pre-crisis level in the coming quarters.crisis, employment,unemployment

Different lineages of freshwater jellyfishes (Cnidaria, Olindiidae, Craspedacusta) invading Europe: another piece of the puzzle from Sicily, Italy

Correctly identifying and mapping the distribution patterns of passively dispersing freshwater organisms is an important task to understand the mechanisms through which these organisms move across the world and to counteract the introduction of invasive alien species in a timely way; moreover, in the light of the growing evidence of the pervasiveness of cryptic species and cryptic biological invasions among all animal groups, the use of molecular identification tools is strongly advisable. In this context, we present the first record of the freshwater jellyfish Craspedacusta sowerbii Lankester, 1880 in the Mediterranean island of Sicily, Italy. This invasive species, native to China, was found during a routine survey campaign aimed at assessing the freshwater species richness in the Sicilian region. Individuals were collected in November 2017 and identified to species level through the sequencing of fragments of both mitochondrial and nuclear genes, and their comparison to the sequences available on public databases. The analysis of molecular data proved that the Sicilian population belongs to C. sowerbii s.s., i.e. one of the two major Craspedacusta lineages which have been discovered to have invaded Europe and which were until recently erroneously jointly reported under the binomen Craspedacusta sowerbii. In order to get a clearer picture of the history and patterns of freshwater jellyfish invasion worldwide, the need for molecular identification of all the non-native Craspedacusta populations currently reported as C. sowerbii, and the opportunity of monitoring human-impacted ecosystems is stressed

Indol-3-ylglyoxylamide as Privileged Scaffold in Medicinal Chemistry

In recent years, indolylglyoxylamide-based derivatives have received much attention due to their application in drug design and discovery, leading to the development of a wide array of compounds that have shown a variety of pharmacological activities. Combining the indole nucleus, already validated as a "privileged structure," with the glyoxylamide function allowed for an excellent template to be obtained that is suitable to a great number of structural modifications aimed at permitting interaction with specific molecular targets and producing desirable therapeutic effects. The present review provides insight into how medicinal chemists have elegantly exploited the indolylglyoxylamide moiety to obtain potentially useful drugs, with a particular focus on compounds exhibiting activity in in vivo models or reaching clinical trials. All in all, this information provides exciting new perspectives on existing data that can be useful in further design of indolylglyoxylamide-based molecules with interesting pharmacological profiles. The aim of this report is to present an update of collection data dealing with the employment of this moiety in the rational design of compounds that are able to interact with a specific target, referring to the last 20 years

Essential Principles and Recent Progress in the Development of TSPO PET Ligands for Neuroinflammation Imaging

The translocator protein 18kDa (TSPO) is expressed in the outer mitochondrial membrane and is implicated in several functions, including cholesterol transport and stereoidogenesis. Under normal physiological conditions, TSPO is present in very low concentrations in the human brain but is markedly upregulated in response to brain injury and inflammation. This upregulation is strongly associated with activated microglia. Therefore, TSPO is particulary suited for assessing active gliosis associated with brain lesions following injury or disease. For over three decades, TSPO has been studied as a biomarker. Numerous radioligands for positron emission tomography (PET) that target TSPO have been developed for imaging inflammatory progression in the brain. Although [¹¹C]PK11195, the prototypical first-generation PET radioligand, is still widely used for in vivo studies, mainly now as its single more potent R-enantiomer, it has severe limitations, including low sensitivity and poor amenability to quantification. Second-generation radioligands are characterized by higher TSPO specific signals but suffer from other drawbacks, such as sensitivity to the TSPO single nucleotide polymorphism (SNP) rs6971. Therefore, their applications in human studies have a burden of needing to genotype subjects. Consequently, recent efforts are focused on developing improved radioligands that combine the optimal features of the second-generation with the ability to overcome the differences in binding affinities across the population. This review presents essential principles in the design and development of TSPO PET ligands and discusses prominent examples among the main chemotypes

First assessment of entomopathogenic fungi and nematodes in hot springs in central Italy and first record of Pristionchus uniformis for the country

Geothermal soils in Central Italy are characterized by high temperatures (up to 60°C), low pH values, and sparse vegetation. The biodiversity of entomopathogenic soil fungi and nematodes has never been assessed in these areas. Soil samples (N = 12) separated from each other by at least 100 meters were collected in the areas of Le Biancane Natural Park (Grosseto) and Sasso Pisano (Pisa). The Galleria bait method was used to assess the presence of entomopathogenic fungi and nematodes. A total of nine species of fungi and one nematode were isolated and molecularly identified. All these species came from samples collected in the immediate surroundings of geothermal spring, apart from one Beauveria pseudobassiana collected at about 16 meters from the nearest spring. Moreover, Pristionchus uniformis previously reported only from North America and few European countries is recorded for the first time in Italy.&nbsp

Tetrahydroquinazole-based secondary sulphonamides as carbonic anhydrase inhibitors: synthesis, biological evaluation against isoforms I, II, IV, and IX, and computational studies

A library of variously decorated N-phenyl secondary sulphonamides featuring the bicyclic tetrahydroquinazole scaffold was synthesised and biologically evaluated for their inhibitory activity against human carbonic anhydrase (hCA) I, II, IV, and IX. Of note, several compounds were identified showing submicromolar potency and excellent selectivity for the tumour-related hCA IX isoform. Structure–activity relationship data attained for various substitutions were rationalised by molecular modelling studies in terms of both inhibitory activity and selectivity

Exploiting the 4-Phenylquinazoline Scaffold for the Development of High Affinity Fluorescent Probes for the Translocator Protein (TSPO)

The quinazoline class was exploited to search for a new Translocator Protein (TSPO) fluorescent probe endowed with improved affinity and Residence Time (RT). Computational studies on an "in-house" collection of quinazoline derivatives, featuring highly steric demanding groups at the amide nitrogen, suggested that, despite their molecular extension, these ligands are still easily lodged in the TSPO binding site. Binding assays supported this hypothesis, highlighting a low nanomolar/subnanomolar affinity of these ligands, together with a higher RT of the representative compound 11 with respect to our previously reported indole-based fluorescent probe. Thanks to the amenability of the amide nitrogen atom to be substituted with bulky groups, we developed quinazoline-based imaging tools by fluorescently labeling the scaffold at this position. Probes with relevant TSPO affinity, favorable spectroscopic properties, and improved RT were identified. Results from fluorescence microscopy showed that these probes specifically labeled the TSPO at mitochondrial level in U343 cell line

Beavers are not alone: parasitic assessment of released Eurasian beavers in Central Italy

The Eurasian beaver Castor fiber L., 1758, absent in Italy for over 500 years, has established reproductive populations in Central Italy since 2019, most likely following unauthorized releases. Besides positive effects on local biodiversity, beavers may host a plethora of infective agents, including parasites. Therefore, an assessment of parasitic load of released beavers is pivotal to predict potential risks for other species and human health, following beaver releases. Microscopical and molecular analyses on collected beaver feces confirmed the presence of Giardia duodenalis, Trichuris spp., and Travassosius rufus, a species of nematode recorded in Italy for the first time. The necropsy carried out on a road-killed adult female beaver provided us the first records for Italy of the beaver beetle Platypsyllus castoris, and of the ectoparasite "fur mites," belonging to the genus Schizocarpus. The bacterium Bordetella bronchiseptica was isolated for the first time from the bronchi of C. fiber, and it may represent a threat to wild and domestic animals and human health. The dipteran species Chrysomya albiceps was also recorded for the first time on a beaver; this blowfly can cause myiasis in livestock and humans. Although the acquired data represent a first assessment for Italy, the high number of pathogens found in these few samples should be evaluated in terms of disease risk analysis

Inhibition studies on carbonic anhydrase isoforms I, II, IV and IX with N-arylsubstituted secondary sulfonamides featuring a bicyclic tetrahydroindazole scaffold

Carbonic Anhydrases (CAs) are pharmaceutically relevant targets for the treatment of several disease conditions. The ubiquitous localization of these enzymes and the high homology shared by the different isoforms represent substantial impediments for the discovery of potential drugs devoid of off-target side effects. As a consequence, substantial efforts are still needed to allow for the full realization of the pharmacological potential of CA modulators. In this contribution, starting from our previous studies, we describe the synthesis of a set of new bicyclic tetrahydroindazoles featuring a secondary sulfonamide. Biological evaluation of the inhibitory activity against the hCA I, II, IV, and IX isoforms allowed drawing a structure-activity relationship profile that was rationalized through theoretical studies. This allowed dissecting the new molecules into the single portions influencing the zinc chelation properties and the selectivity profile thereby offering a new platform for the discovery of new isotype selective CA inhibitors