Conflito escolar, diálogo e mediação de conflitos: interseções e contribuições pós-pandemia

Este trabalho, que se propõe analítico e interdisciplinar, dispõe sobre a possibilidade da utilização da mediação de conflito

Stress and coping among patients' relatives in the transoperative of cardiac surgery

Objective: to evaluate stress and coping among relatives of patients in the perioperative cardiac surgery. Method: it is a quantitative, analytical and cross-sectional study developed in a postage hospital IV, with 53 relatives waiting in the waiting room of the surgical center. Data were collected between March and April 2013 through a socio-demographic questionnaire and Symptom Inventory of Stress and Coping Inventory of Jalowiec. A Research Ethics Committee, approved the research project, number 198.527. Results: 60% are women, aged between 18 and 58 years old, married, with children, catholic, most of them daughters of patients. As for the stages of stress, 60% were in the intermediate phase and the majority used the sustaintive coping style. Conclusion: the results may support health professionals - researchers and managers - and mobilize integrated actions towards qualified assistance in the perioperative, with emphasis on care to patients and families

Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs by using peptides selected from hypothetical proteins identified by an immunoproteomic approach

In Brazil, the percentage of infected dogs living in areas where canine visceral leishmaniasis (CVL) is endemic ranges from 10 to 62%; however, the prevalence of infection in dogs is probably higher than figures reported from serological studies. In addition, problems with the occurrence of false-positive or false-negative results in the serodiagnosis of CVL have been reported. The present work analyzed the potential of synthetic peptides mapped from hypothetical proteins for improvement of the serodiagnosis of Leishmania infantum infection in dogs. From 26 identified leishmanial proteins, eight were selected, considering that no homologies between these proteins and others from trypanosomatide sequence databases were encountered. The sequences of these proteins were mapped to identify linear B-cell epitopes, and 17 peptides were synthesized and tested in enzyme-linked immunosorbent assays (ELISAs) for the serodiagnosis of L. infantum infection in dogs. Of these, three exhibited sensitivity and specificity values higher than 75% and 90%, respectively, to differentiate L. infantum-infected animals from Trypanosoma cruziinfected animals and healthy animals. Soluble Leishmania antigen (SLA) showed poor sensitivity (4%) and specificity (36%) to differentiate L. infantum-infected dogs from healthy and T. cruzi-infected dogs. Lastly, the three selected peptides were combined in different mixtures and higher sensitivity and specificity values were obtained, even when sera from T. cruzi-infected dogs were used. The study’s findings suggest that these three peptides can constitute a potential tool for more sensitive and specific serodiagnosis of L. infantum infection in dogsThis work was supported by grants from the Pró-Reitoria de Pesquisa from UFMG (Edital 07/2012), Instituto Nacional de Ciência e Tecnologia em Nano-biofarmacêutica (INCT-NANOBIOFAR, Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) (CBB-APQ-02364-08, CBB-APQ-00356-10, CBB-APQ-00496-11, and CBB-APQ-00819-12), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (APQ-472090/2011-9), and the Instituto Nacional de Ciência e Tecnologia em Vacinas (INCT-V). E.A.F.C. and A.P.F. are CNPq grant recipients. M.A.C.-F. is a FAPEMIG/CAPES grant recipient. This study was supported in Spain, in part, by grants from the Ministerio de Ciencia e Innovación (FIS/PI1100095)

Subtractive phage display selection from canine visceral leishmaniasis identifies novel epitopes that mimic leishmania infantum antigens with potential serodiagnosis applications

Visceral leishmaniasis (VL) is a zoonotic disease that is endemic to Brazil, where dogs are the main domestic parasite reservoirs, and the percentages of infected dogs living in regions where canine VL (CVL) is endemic have ranged from 10% to 62%. Despite technological advances, some problems have been reported with CVL serodiagnosis. The present study describes a sequential subtractive selection through phage display technology from polyclonal antibodies of negative and positive sera that resulted in the identification of potential bacteriophage-fused peptides that were highly sensitive and specific to antibodies of CVL. A negative selection was performed in which phage clones were adhered to purified IgGs from healthy and Trypanosoma cruzi-infected dogs to eliminate cross-reactive phages. The remaining supernatant nonadhered phages were submitted to positive selection against IgG from the blood serum of dogs that were infected with Leishmania infantum. Phage clones that adhered to purified IgGs from the CVL-infected serum samples were selected. Eighteen clones were identified and their reactivities tested by a phage enzyme-linked immunosorbent assay (phage-ELISA) against the serum samples from infected dogs (n 31) compared to those from vaccinated dogs (n 21), experimentally infected dogs with cross-reactive parasites (n 23), and healthy controls (n 17). Eight clones presented sensitivity, specificity, and positive and negative predictive values of 100%, and they showed no crossreactivity with T. cruzi- or Ehrlichia canis-infected dogs or with dogs vaccinated with two different commercial CVL vaccines in Brazil. Our study identified eight mimotopes of L. infantum antigens with 100% accuracy for CVL serodiagnosis. The use of these mimotopes by phage-ELISA proved to be an excellent assay that was reproducible, simple, fast, and inexpensive, and it can be applied in CVL-monitoring programsThis work was supported by grants from the Pró-Reitoria de Pesquisa of UFMG (supported 03/2013), the Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica (INCT Nano-Biofar), Rede Nanobiotec/Brasil-UFU (CAPES), PRONEX-FAPEMIG (APQ-01019- 09), FAPEMIG (APQ-00496-11 and APQ-00819-12), and CNPq (APQ- 472090/2011-9 and APQ-482976/2012-8). E.A.F.C. and L.R.G. are recipients of grants from CNPq. M.A.C.-F. is the recipient of a grant from FAPEMIG/CAPE

Deforestation, degradation and violence in the “Gurupi Mosaic” – The most threatened region of Amazonia

O "Mosaico Gurupi" está localizado entre o oeste do Maranhão e o leste do Pará, na Área de Endemismo Belém, que embora seja a região mais desmatada do Bioma Amazônico no Brasil, preserva uma diversidade cultural e biológica superlativa. O mosaico engloba seis Terras Indígenas (Alto Turiaçu, Awá, Caru, Arariboia, Rio Pindaré e Alto Rio Guamá) e uma Unidade de Conservação (Reserva Biológica do Gurupi). Essas áreas protegidas conservam os principais remanescentes florestais da região e garantem a manutenção de serviços ecossistêmicos essenciais aos dois estados, principalmente de regulação hidrológica. No entanto, essa região vive sob ameaças constantes de desmatamento e de degradação pela extração ilegal de madeira, e por incêndios criminosos. Os povos indígenas e lideranças comunitárias da região são vitimados pela violência associada a tais crimes. Para promover a conservação e a restauração dessas áreas, uma rede formada por diversas instituições indígenas e não indígenas vem trabalhando em conjunto para o reconhecimento do "Mosaico Gurupi" pelo Ministério do Meio Ambiente. A proposta aqui apresentada inclui o mosaico em questão e o Corredor Ecológico da Amazônia Maranhense, que irá conectar os principais remanescentes florestais da região, por meio da restauração das matas ciliares ao longo dos rios Buriticupu, Pindaré e Zutiua. A conformação do mosaico visa integrar esforços para a proteção territorial, a restauração florestal e o fortalecimento da cultura e educação indígena; o que poderá converter a região mais ameaçada da Amazônia em um exemplo de conservação e sustentabilidade econômica e social por meio da promoção da restauração florestal.The "Gurupi Mosaic" is located between west Maranhão and east Pará states, in the Belém Endemism Area, which although being the most deforested region of the Amazonian biome in Brazil, preserves superlative biological and cultural diversity. The Mosaic includes six indigenous lands (Alto Turiaçu, Awá, Caru, Araribóia, Rio Pindaré, Alto Rio Guamá) and a conservation unit (Gurupi Biological Reserve). These protected areas conserve the region’s main forest remnants and guarantee the maintenance of essential ecosystem services. However, this region is under constant threats of deforestation and degradation by illegal logging and arson. Indigenous peoples living in the region are victims of the violence associated with such crimes. To promote the conservation and restoration of these areas, several indigenous and non-indigenous institutions have been working together to formalize the "Gurupi Mosaic" with the Ministry of the Environment. The proposal presented here includes the Mosaic and one ecological corridor in the state of Maranhão, which will connect the region’s last forest remnants through the restoration of the riparian forests along the main rivers. The conformation of the Mosaic aims to integrate efforts to protect territories, restore forests and strengthen indigenous culture and education. This could turn the most threatened region of Amazonia into an example of conservation and economic and social sustainability

Acinic cell carcinoma of the oral and maxillofacial region: an international multicenter study

Abstract The aim of this study was to describe the prevalence, clinicopathological, and prognostic features of acinic cell carcinoma (AciCC) of the oral and maxillofacial region. AciCC cases were retrospectively retrieved from 11 pathology centers of three different countries. Medical records were examined to extract demographic, clinical, pathologic, and follow-up information. A total of 75 cases were included. Females (65.33%) with a mean age of 45.51 years were mostly affected. The lesions usually presented as an asymptomatic (64.28%) nodule (95.66%) in the parotid gland (70.68%). The association of two histopathological patterns was the most common finding (48.93%) and the tumors presented mainly conventional histopathological grades (86.11%). Surgical treatment was performed in the majority of the cases (59.19%). Local recurrence was observed in 20% of the informed cases, regional metastasis in 30.43%, and distant metastasis in 12.50%. The statistical analysis showed that the cases with a solid histopathological pattern (p=0.01), high-grade transformation (p=0.008), recurrence (p=0.007), and regional metastasis (p=0.03) were associated with poor survival. In conclusion, high histopathological transformation, presence of nodal metastasis, and recurrence were prognostic factors for AciCC of the oral and maxillofacial region