Les automates d’immuno-analyse pour la recherche et le dosage d’immunoglobulines E spécifiques unitaires

The detection and assay of specific IgE is done by immunological techniques using allergenic extracts assubstrates as well as molecular allergens used as specific and/or predictive markers of clinical allergy. InFrance, four automated immunoassay instruments share the market for performing this assay routinelyin the medical laboratory: the Phadia TM from Thermo Fisher Scientific® , the Immulite TM from Siemens® ,the NOVEOS TM from HYCOR Biomedical® and the iSYS TM from IDS® . The purpose of this article is todescribe the characteristics of routinely available immunoassay instruments for the determination ofspecific IgE and to show that although the different techniques are equivalent to each other in termsof repeatability, reproducibility and linearity, the specific IgE results obtained are correlated but notquantitatively identical. Therefore, when monitoring specific IgE levels, it is essential to use the sametechnique on the same instrument.La détection et le dosage des IgE spécifiques se fait par technique immunologique en utilisant commesubstrat des extraits allergéniques ainsi que des allergènes moléculaires utilisés comme marqueurs spé-cifiques et/ou prédictifs d’allergie clinique. En France, quatre automates d’immuno-analyse se partagentle marché pour effectuer ce dosage en routine au laboratoire d’analyses médicales : le Phadia TM dela société Thermo Fisher Scientific® , l’Immulite TM de la société Siemens® , le NOVEOS TM de la sociétéHYCOR Biomedical® et l’iSYS TM de la société IDS® . Cet article a pour but de décrire les caractéristiquesdes automates d’immuno-analyse disponibles en routine pour le dosage des IgE spécifiques unitaires etde montrer que bien que les différentes techniques soient équivalentes entre elles en termes de répétabi-lité, reproductibilité et linéarité, les résultats d’IgE spécifiques obtenus sont corrélés mais non identiquesquantitativement. De ce fait, dans le cadre d’un suivi du taux d’IgE spécifiques, il est indispensabled’utiliser la même technique sur le même automat

Apomorphine-induced inhibition of substantia nigra dopamine neurons: Effects of unilateral injection through the internal carotid artery

International audiencePossible indirect components in the inhibition of firing of A9 dopamine neurons induced by systemic apomorphine were studied using unilateral drug administration through the internal carotid artery, known to irrigate only the ipsilateral mid- and forebrain. When compared to intravenous injection, unilateral intracarotid administration inhibited ipsilateral neurons with a marked decrease of both the latency (less than 1 s) and the dose required for complete inhibition, whereas contralateral neurons were not affected. This suggests a first-pass central effect of apomorphine, presumably associated with brain extraction. Thus, peripheral and hindbrain targets do not seem to contribute to the inhibitory effect of low doses of systemic apomorphine. An intranigral possible mode of action is discussed in view of the particular arrangement of dopaminergic dendrites within the zona reticulata

The importance of EN ISO 15189 accreditation of allergen-specific IgE determination for reliable in vitro allergy diagnosis

International audienceBackground: Allergen-specific serum immunoglobulin E detection and quantification have become an important step in allergy diagnosis and follow-up. In line with the current trend of laboratory test accreditation to international standards, we set out to design and assess an accreditation procedure for allergen-specific serum IgE.Methods: Method validation according to the accreditation procedure under the EN ISO 15189 standard was carried out for allergen-specific immunoglobulin E determination using the fluoroimmunoenzymatic method ImmunoCAP(®) (ThermoFisher). Data were produced by 25 hospital laboratories in France. A total of 29 allergen specificities including mixes, extracts, and molecular allergens were assayed. Allergen-specific serum immunoglobulin E concentrations ranged from 0.1 to 100 kUA /l.Results: Repeatability, reproducibility, and accuracy results fulfilled method validation criteria for automated laboratory tests and proved similar irrespective of the allergen specificity, allergen-specific serum immunoglobulin E concentration, or individual laboratory.Conclusion: Allergen-specific serum immunoglobulin E determination with the fluoroimmunoenzymatic method ImmunoCAP(®) is a highly repeatable, reproducible, and accurate method which may be considered as a single analyte assay in view of the EN ISO 15189 accreditation procedure

Pru p 7 et allergie à la pêche : distribution géographique, implications cliniques et lien avec la pollinose au cyprès

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